##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146686_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4383607
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49597306510369	31.0	31.0	34.0	28.0	34.0
2	31.512528837553184	31.0	31.0	34.0	28.0	34.0
3	31.675729827057946	33.0	31.0	34.0	28.0	34.0
4	35.11873372772696	37.0	35.0	37.0	32.0	37.0
5	35.29554154831854	37.0	35.0	37.0	32.0	37.0
6	35.36184835912526	37.0	35.0	37.0	32.0	37.0
7	35.32278691953909	37.0	35.0	37.0	32.0	37.0
8	35.31764435087361	37.0	35.0	37.0	32.0	37.0
9	36.86993154267707	39.0	37.0	39.0	32.0	39.0
10-11	36.700426155903116	39.0	37.0	39.0	32.0	39.0
12-13	36.66166412728148	39.0	37.0	39.0	32.0	39.0
14-15	37.86009261322924	40.0	38.0	41.0	32.0	41.0
16-17	37.86805683082448	40.0	38.0	41.0	32.0	41.0
18-19	37.817109061099686	40.0	38.0	41.0	32.0	41.0
20-21	37.878502452432436	40.0	38.0	41.0	32.0	41.0
22-23	37.79523757490122	40.0	37.5	41.0	32.0	41.0
24-25	37.746697183392584	40.0	37.0	41.0	32.0	41.0
26-27	37.780176233864026	40.0	37.0	41.0	32.0	41.0
28-29	37.74659612506322	40.0	37.0	41.0	32.0	41.0
30-31	37.59482898444135	40.0	37.0	41.0	31.0	41.0
32-33	37.595428148554376	40.0	37.0	41.0	31.0	41.0
34-35	37.52660947936254	40.0	37.0	41.0	31.0	41.0
36-37	37.401490256768	40.0	37.0	41.0	31.0	41.0
38-39	37.29669493638458	40.0	36.0	41.0	30.0	41.0
40-41	37.21034789386914	40.0	36.0	41.0	30.0	41.0
42-43	37.028163108599834	40.0	36.0	41.0	30.0	41.0
44-45	36.874277621146234	39.0	35.0	41.0	30.0	41.0
46-47	36.870454057583174	39.0	35.0	41.0	30.0	41.0
48-49	36.72473444813826	39.0	35.0	41.0	30.0	41.0
50-51	36.54612491493877	39.0	35.0	41.0	29.5	41.0
52-53	36.33672475657603	39.0	35.0	40.5	29.0	41.0
54-55	36.09777530239367	38.0	35.0	40.0	29.0	41.0
56-57	35.85526633660362	38.0	34.0	40.0	28.5	41.0
58-59	35.55559428571037	37.0	34.0	40.0	28.0	41.0
60-61	35.28025379556151	37.0	34.0	40.0	28.0	41.0
62-63	34.95591347490776	36.0	34.0	39.5	27.5	41.0
64-65	34.788389903565715	36.0	34.0	39.0	28.0	41.0
66-67	34.504559829382515	35.0	33.0	39.0	27.5	41.0
68-69	34.35907746748283	35.0	33.0	39.0	27.0	41.0
70-71	34.232627377408605	35.0	33.0	37.5	28.0	40.0
72-73	33.98901464022664	35.0	33.0	37.0	27.5	40.0
74-75	33.71871474792334	35.0	33.0	37.0	27.0	39.0
76-77	33.45407446424828	35.0	33.0	36.0	27.0	39.0
78-79	33.205687804586496	35.0	33.0	36.0	26.0	39.0
80-81	33.021475579357364	35.0	33.0	35.5	26.0	37.0
82-83	32.79483208690925	35.0	33.0	35.0	26.0	37.0
84-85	32.63786580320727	35.0	33.0	35.0	26.0	36.5
86-87	32.48648168049736	35.0	33.0	35.0	26.0	36.0
88-89	32.37492970971166	35.0	33.0	35.0	25.0	36.0
90-91	32.255593510093405	35.0	32.5	35.0	25.0	36.0
92-93	32.13789785443814	35.0	32.0	35.0	25.0	35.5
94-95	32.07831279126984	35.0	32.0	35.0	25.0	35.0
96-97	31.99790674665863	35.0	32.0	35.0	25.0	35.0
98-99	31.92141323343995	35.0	32.0	35.0	25.0	35.0
100	30.98083222332659	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4405060184199243
1101	2	0.46329026195834544
1101	3	0.5076720864680659
1101	4	0.5513692275363056
1101	5	0.45658232482591643
1101	6	0.455063188158114
1101	7	0.4346495725432078
1101	8	0.49198938179537066
1101	9	0.5740102340540076
1101	10-11	0.6404009333407501
1101	12-13	0.5609590046502788
1101	14-15	0.7397331904534852
1101	16-17	0.7417957003188462
1101	18-19	0.7546970012269938
1101	20-21	0.7593981554255862
1101	22-23	0.7299002156132701
1101	24-25	0.7970925340835819
1101	26-27	0.7633512417159309
1101	28-29	0.7701922946177078
1101	30-31	0.8209976721541423
1101	32-33	0.7918153449835827
1101	34-35	0.7866101978504574
1101	36-37	0.7981769070698519
1101	38-39	0.7249896893216743
1101	40-41	0.7207372305611486
1101	42-43	0.7004859172379057
1101	44-45	0.7408386104362776
1101	46-47	0.7157957550975311
1101	48-49	0.7261811222651957
1101	50-51	0.7232823237926027
1101	52-53	0.6382505895869954
1101	54-55	0.6671591720233607
1101	56-57	0.6880122612210116
1101	58-59	0.6981480973311491
1101	60-61	0.6503619505058538
1101	62-63	0.6065351230807536
1101	64-65	0.6367679927386476
1101	66-67	0.7062884475904809
1101	68-69	0.6793265114572122
1101	70-71	0.63853538128118
1101	72-73	0.6212361581034216
1101	74-75	0.5746043526183655
1101	76-77	0.6300256332313765
1101	78-79	0.6523521296321135
1101	80-81	0.5936535215375756
1101	82-83	0.5655492128474293
1101	84-85	0.5334333589074305
1101	86-87	0.566272263765697
1101	88-89	0.5888392453485736
1101	90-91	0.6066589252692083
1101	92-93	0.49310179543618204
1101	94-95	0.5398821681924062
1101	96-97	0.542556979797638
1101	98-99	0.5039863704027283
1101	100	0.5884054050008132
1102	1	0.28880872824099413
1102	2	0.29856979040102516
1102	3	0.31703457396772095
1102	4	0.4421791767478922
1102	5	0.3351574924295022
1102	6	0.38022429419650905
1102	7	0.33399001413401663
1102	8	0.40270971402145506
1102	9	0.509719876210653
1102	10-11	0.6151824932969987
1102	12-13	0.5081416537238468
1102	14-15	0.5807456332596175
1102	16-17	0.5771417056646868
1102	18-19	0.6384491881440866
1102	20-21	0.619179563238518
1102	22-23	0.6156249748665061
1102	24-25	0.6603060747695579
1102	26-27	0.6556136018727727
1102	28-29	0.6592756460442786
1102	30-31	0.6388380882352109
1102	32-33	0.5619883610977325
1102	34-35	0.6588006228198893
1102	36-37	0.6941521359654601
1102	38-39	0.7104933488830554
1102	40-41	0.5864016512646231
1102	42-43	0.7069869265314495
1102	44-45	0.693605519563711
1102	46-47	0.65080882683894
1102	48-49	0.6340938098504623
1102	50-51	0.6097091443474625
1102	52-53	0.6250294503150116
1102	54-55	0.606197206478214
1102	56-57	0.5046864502113664
1102	58-59	0.5418738530658587
1102	60-61	0.5037675526742049
1102	62-63	0.49112505624979264
1102	64-65	0.5248273224524169
1102	66-67	0.5401061562252849
1102	68-69	0.46215923800946257
1102	70-71	0.40478922003992324
1102	72-73	0.44881587662228384
1102	74-75	0.4633610106041033
1102	76-77	0.47750153567571374
1102	78-79	0.4733534087798503
1102	80-81	0.4822840464274236
1102	82-83	0.47907513063193363
1102	84-85	0.5472828471911413
1102	86-87	0.5236626279235637
1102	88-89	0.5537329486517208
1102	90-91	0.5185053324792861
1102	92-93	0.4711842217627904
1102	94-95	0.5542432812545641
1102	96-97	0.5983177747086827
1102	98-99	0.5043102469044598
1102	100	0.5643335592690981
1103	1	0.2836890233220082
1103	2	0.2869603993138732
1103	3	0.308242870126751
1103	4	0.40943614048896393
1103	5	0.25814900127603124
1103	6	0.35440404489603594
1103	7	0.35783455759249705
1103	8	0.3384900264529236
1103	9	0.4054144756397662
1103	10-11	0.500356618750466
1103	12-13	0.5152329910915228
1103	14-15	0.6138073850213033
1103	16-17	0.5997599926024364
1103	18-19	0.6255574488095519
1103	20-21	0.5644953591091095
1103	22-23	0.5890487257879755
1103	24-25	0.5884526830201011
1103	26-27	0.5532380539463304
1103	28-29	0.5227228420964778
1103	30-31	0.5026223851058731
1103	32-33	0.5116278437723807
1103	34-35	0.5443966747549993
1103	36-37	0.5836059115386618
1103	38-39	0.6464542117202328
1103	40-41	0.5765815257072333
1103	42-43	0.5929720976183788
1103	44-45	0.45075432480442856
1103	46-47	0.4462025015857449
1103	48-49	0.4527789273007059
1103	50-51	0.4264296752185004
1103	52-53	0.43395978163822235
1103	54-55	0.48680505804287577
1103	56-57	0.45983679365347285
1103	58-59	0.43609955384363985
1103	60-61	0.39089866837883847
1103	62-63	0.38508752774705357
1103	64-65	0.3259302432295428
1103	66-67	0.3898744021648852
1103	68-69	0.36847306367880606
1103	70-71	0.383580547367238
1103	72-73	0.3843162808790481
1103	74-75	0.33024763683921776
1103	76-77	0.3084276403579551
1103	78-79	0.3935139092831932
1103	80-81	0.25007971092777836
1103	82-83	0.3399859237590377
1103	84-85	0.3468496032816333
1103	86-87	0.3985876004226583
1103	88-89	0.4527081709709293
1103	90-91	0.4223029570524659
1103	92-93	0.3803282300874784
1103	94-95	0.318565937600205
1103	96-97	0.42442742208933737
1103	98-99	0.44945947401661357
1103	100	0.4134320622860521
1104	1	0.2426422065703875
1104	2	0.3142997673411223
1104	3	0.2849899387388568
1104	4	0.38345273716247164
1104	5	0.25701413645395377
1104	6	0.25767734896686534
1104	7	0.2568158157295457
1104	8	0.33119483326328236
1104	9	0.3929088295269807
1104	10-11	0.5276465143654789
1104	12-13	0.3904367025329165
1104	14-15	0.5155703827755787
1104	16-17	0.485124809197508
1104	18-19	0.43509700965866926
1104	20-21	0.44793665035906827
1104	22-23	0.4560297837784617
1104	24-25	0.46347623947973915
1104	26-27	0.39040962406681956
1104	28-29	0.42786231275140807
1104	30-31	0.41307553650109696
1104	32-33	0.41520929249490734
1104	34-35	0.41467595767841914
1104	36-37	0.3851790034500411
1104	38-39	0.417190833625952
1104	40-41	0.36375001566353404
1104	42-43	0.44818340201072004
1104	44-45	0.4015594350968783
1104	46-47	0.3781101506208131
1104	48-49	0.25637339939851955
1104	50-51	0.22364946414236897
1104	52-53	0.27813022340814086
1104	54-55	0.3851161432383208
1104	56-57	0.31536146179335134
1104	58-59	0.23045498242655782
1104	60-61	0.2946384211265425
1104	62-63	0.31953084949459054
1104	64-65	0.25362804388459637
1104	66-67	0.23163033888000228
1104	68-69	0.18561333358633192
1104	70-71	0.2368655309282488
1104	72-73	0.2876763989029385
1104	74-75	0.24756423268176775
1104	76-77	0.26279289212560286
1104	78-79	0.20743899815144573
1104	80-81	0.2637740798542012
1104	82-83	0.27598621592581907
1104	84-85	0.2884185809553941
1104	86-87	0.2532328573237379
1104	88-89	0.2288166402478211
1104	90-91	0.26555646651613785
1104	92-93	0.2597603324459783
1104	94-95	0.3269929436590786
1104	96-97	0.28556174627977526
1104	98-99	0.21806062655711145
1104	100	0.36040461225267606
1105	1	0.0881841845851028
1105	2	0.20250575372589097
1105	3	0.19178567579005446
1105	4	0.2304472253882892
1105	5	0.13463252915340007
1105	6	0.1804348642184621
1105	7	0.21500117030285537
1105	8	0.17302218532679348
1105	9	0.3384570059610965
1105	10-11	0.3376745611682921
1105	12-13	0.19036535801264876
1105	14-15	0.3843755254426142
1105	16-17	0.30058022251122907
1105	18-19	0.3421662909978025
1105	20-21	0.3196391422589002
1105	22-23	0.341102765433817
1105	24-25	0.326824166841142
1105	26-27	0.32438359842572595
1105	28-29	0.31030815790368393
1105	30-31	0.3743986165357782
1105	32-33	0.34200737319282126
1105	34-35	0.39107144968428287
1105	36-37	0.32731109105433376
1105	38-39	0.3115176177878709
1105	40-41	0.32547315314361214
1105	42-43	0.27615447149671724
1105	44-45	0.2811628218188176
1105	46-47	0.3434089966796847
1105	48-49	0.23484582905770424
1105	50-51	0.25576334963693625
1105	52-53	0.3062213076690483
1105	54-55	0.33355464927516465
1105	56-57	0.32559695526067856
1105	58-59	0.3414315963876433
1105	60-61	0.3582075024861169
1105	62-63	0.27036093488649016
1105	64-65	0.3103364879635606
1105	66-67	0.2600950862855598
1105	68-69	0.21150806042794557
1105	70-71	0.2294260999475668
1105	72-73	0.28039351427804604
1105	74-75	0.22315074613085528
1105	76-77	0.21929470337455825
1105	78-79	0.29815215297997355
1105	80-81	0.18295407937179675
1105	82-83	0.19032944446242794
1105	84-85	0.14497786219838105
1105	86-87	0.15747276059397564
1105	88-89	0.1644017119066845
1105	90-91	0.17585519566313224
1105	92-93	0.28218843158007445
1105	94-95	0.22783506322936375
1105	96-97	0.1738800344518907
1105	98-99	0.17839624994537928
1105	100	0.24473608247865997
1106	1	0.10322063632607481
1106	2	0.1588963998238988
1106	3	0.11855141480215536
1106	4	0.3178267054206785
1106	5	0.17421231933933257
1106	6	0.1923594510713187
1106	7	0.20554262236954202
1106	8	0.19601370881037639
1106	9	0.29245025371790234
1106	10-11	0.3521141921247377
1106	12-13	0.26298055666941167
1106	14-15	0.38425956951414975
1106	16-17	0.2826156693722339
1106	18-19	0.3299112583093873
1106	20-21	0.3226519852585952
1106	22-23	0.40488213186176836
1106	24-25	0.4285242159391558
1106	26-27	0.37014934702941815
1106	28-29	0.3758580775853986
1106	30-31	0.45432119262905246
1106	32-33	0.3500612764200284
1106	34-35	0.34881311801162695
1106	36-37	0.3781773058784452
1106	38-39	0.3765709047968997
1106	40-41	0.3894567038717085
1106	42-43	0.3960446546635339
1106	44-45	0.42324872378897993
1106	46-47	0.3956613870390342
1106	48-49	0.30009282815660043
1106	50-51	0.27784549255960656
1106	52-53	0.2849544739182619
1106	54-55	0.2908452866455349
1106	56-57	0.38861952599170735
1106	58-59	0.361238758727346
1106	60-61	0.27903554586572454
1106	62-63	0.33448120746003696
1106	64-65	0.2888347434956344
1106	66-67	0.3255835531332636
1106	68-69	0.33888512423580863
1106	70-71	0.24331082276113847
1106	72-73	0.1627830241888404
1106	74-75	0.18895592117824833
1106	76-77	0.18058491585880176
1106	78-79	0.16451714620885838
1106	80-81	0.2096053547453991
1106	82-83	0.27413515183130954
1106	84-85	0.23704102638195934
1106	86-87	0.21732554797385717
1106	88-89	0.2701485205660461
1106	90-91	0.28523327894066597
1106	92-93	0.2560796166994024
1106	94-95	0.26161355832181243
1106	96-97	0.2268457370349708
1106	98-99	0.2348408683871881
1106	100	0.2513284816579926
1107	1	0.1002850924661729
1107	2	0.1051887296530083
1107	3	0.1356072420135277
1107	4	0.19794513226786847
1107	5	0.08967719193417167
1107	6	0.1916934594543349
1107	7	0.14366215020743311
1107	8	0.14800685818067194
1107	9	0.1712895870458766
1107	10-11	0.23952931742559969
1107	12-13	0.20405800297516663
1107	14-15	0.28958741539840815
1107	16-17	0.21578820903233975
1107	18-19	0.20349420568661714
1107	20-21	0.16896730846768548
1107	22-23	0.2588054452574653
1107	24-25	0.31059878582554745
1107	26-27	0.15777154450389475
1107	28-29	0.2510976399954785
1107	30-31	0.36157580752723106
1107	32-33	0.3256712244671789
1107	34-35	0.30993201357274813
1107	36-37	0.3169308632881851
1107	38-39	0.3589281264474806
1107	40-41	0.26444241682576575
1107	42-43	0.3192344253598023
1107	44-45	0.38992589781623366
1107	46-47	0.31880570269576936
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2216	94-95	-0.9486635614545307
2216	96-97	-0.9963384523220356
2216	98-99	-1.026224230406303
2216	100	-0.6883344368469757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10.0
3	19.0
4	34.0
5	26.0
6	56.0
7	191.0
8	545.0
9	913.0
10	1258.0
11	2055.0
12	3430.0
13	4810.0
14	6658.0
15	9017.0
16	11112.0
17	13039.0
18	15011.0
19	17461.0
20	20243.0
21	23191.0
22	26545.0
23	30603.0
24	35876.0
25	40963.0
26	46350.0
27	52144.0
28	61505.0
29	74769.0
30	87402.0
31	105232.0
32	131580.0
33	171926.0
34	243852.0
35	359236.0
36	594735.0
37	1041202.0
38	902650.0
39	247766.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89230594348369	19.47705140829671	12.260035800323491	24.370606847896102
2	16.258696388667403	19.58537945855677	39.23422729756336	24.92169685521247
3	19.08021804685899	25.296661539605747	28.679586373485776	26.943534040049478
4	12.81420551843654	15.944819284914805	38.24356097990554	32.99741421674312
5	13.865284922084095	37.84124993954525	34.34986280194295	13.943602336427704
6	31.734287880194668	36.762654411041744	17.36883972687647	14.134217981887124
7	28.031435529219873	31.31612895866958	22.55747877018187	18.094956741928677
8	26.87580927595637	34.33102591781817	19.55012241225601	19.24304239396945
9	26.517285163334307	14.969342451591002	20.52481689648612	37.98855548858857
10-11	26.225528188713838	25.17840687409257	27.075938565871642	21.52012637132195
12-13	26.28159320776901	23.020081798130825	27.00545088946941	23.69287410463075
14-15	23.75377058552063	24.341905505585245	24.342464406562364	27.56185950233176
16-17	23.298447891748904	26.94478903801591	25.440906143965236	24.315856926269955
18-19	22.802683948889722	27.337910150720617	26.80629655340283	23.05310934698683
20-21	24.365818688670064	26.601567445674924	25.93017327221109	23.10244059344392
22-23	24.73658384353532	26.14021532280399	25.766158449369208	23.357042384291482
24-25	24.210452475217032	26.207427533132353	26.28061014404975	23.30150984760086
26-27	23.993329011022805	26.437620709494652	25.495236865506637	24.073813413975905
28-29	23.282785946075055	26.751382388895305	26.192335730496875	23.77349593453276
30-31	23.82932475306207	26.588297319722255	26.11470257373918	23.4676753534765
32-33	24.056267003973332	26.711929607271877	25.357292326954006	23.874511061800785
34-35	24.13762856552978	26.622432253936097	26.031370160364002	23.208569020170117
36-37	23.968757001077282	26.11688713453521	25.697050638635133	24.217305225752376
38-39	23.952403315241686	26.363355443994013	26.111640452391168	23.572600788373133
40-41	23.714924527499107	25.672593220050917	26.709299950049637	23.903182302400346
42-43	23.69797219038506	25.883836352910993	26.140933093359642	24.277258363344302
44-45	23.91073997899947	26.427792073736423	26.05810375504307	23.60336419222104
46-47	23.882956125488214	26.32040618615694	25.688404127207654	24.108233561147188
48-49	24.33360040769626	26.09520738991682	25.857704011557324	23.713488190829597
50-51	23.844871828365083	25.967309362511237	26.066560654118486	24.12125815500519
52-53	23.65025145350164	26.377540775626805	25.827857032027236	24.144350738844317
54-55	23.558418981909558	26.23654579332974	25.89029323488144	24.314741989879263
56-57	24.33899790038553	25.93015316747463	25.597431865200303	24.13341706693954
58-59	23.424862489511668	26.174832685828104	26.335458854089723	24.064845970570502
60-61	23.597037358149404	26.561447439345415	25.82881816600263	24.012697036502555
62-63	23.8280254973248	26.22658734581354	25.531022205268318	24.414364951593342
64-65	23.27261440168355	26.238595593845893	25.940189401635067	24.548600602835492
66-67	23.86321764943565	26.04722054972762	25.840364845413205	24.249196955423525
68-69	23.3982573273331	25.973563335342437	26.18292222763154	24.44525710969293
70-71	23.28361780906971	25.785478020418008	26.15096121281103	24.77994295770125
72-73	23.753757782205252	25.89820034828142	26.39981593511827	23.948225934395058
74-75	23.879903716910103	26.08788746545979	26.124721987197923	23.90748683043218
76-77	23.70271768944437	26.01045115120883	25.772118179297166	24.514712980049637
78-79	23.74491168078688	26.045041863275074	25.68806669650871	24.521979759429332
80-81	23.42084465886074	26.344735745805657	26.18567263936616	24.048746955967445
82-83	23.976604118028384	25.595610922321598	26.974001875968657	23.453783083681365
84-85	24.006137609811415	25.93233685580849	26.10688069590302	23.954644838477076
86-87	23.072583956926447	26.365897061507575	26.4874634969885	24.074055484577478
88-89	23.932117554675134	25.37111698295376	26.264471554694296	24.432293907676808
90-91	23.66991286051968	25.624736127206965	26.15586407623675	24.549486936036608
92-93	23.865609791487387	25.589370501611135	26.37820550844171	24.166814198459768
94-95	23.05873509179479	25.58632714181666	26.91951750771254	24.435420258676004
96-97	23.432304382117085	25.49040073681312	26.548360433391032	24.528934447678765
98-99	22.913603371041575	25.914254719423983	26.40428315745406	24.76785875208038
100	23.65084785225476	25.353299409027276	25.77593902316021	25.21991371555775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	203.0
2	152.5
3	202.0
4	205.5
5	185.0
6	181.5
7	186.5
8	221.0
9	241.5
10	217.0
11	201.0
12	197.0
13	197.0
14	201.5
15	241.0
16	276.0
17	318.0
18	385.0
19	480.5
20	620.0
21	857.0
22	1174.5
23	1634.0
24	2354.5
25	3299.0
26	4901.5
27	6926.0
28	9462.0
29	14354.0
30	21980.0
31	30087.5
32	40968.0
33	52245.5
34	61036.5
35	71389.5
36	82754.0
37	98569.5
38	109290.0
39	113084.0
40	113140.5
41	110259.0
42	112757.5
43	122527.5
44	137157.0
45	161081.5
46	184782.0
47	200078.0
48	209089.5
49	208695.0
50	202342.0
51	207255.0
52	213022.5
53	207551.0
54	200372.5
55	180022.0
56	161407.0
57	138153.0
58	114718.5
59	106916.0
60	89926.5
61	68093.0
62	53353.0
63	43729.0
64	32401.5
65	19546.0
66	13448.5
67	10174.5
68	7610.5
69	5635.0
70	4274.0
71	3319.0
72	2493.5
73	1962.0
74	1487.5
75	1074.5
76	736.5
77	404.5
78	230.0
79	156.5
80	118.0
81	83.0
82	63.5
83	53.0
84	45.0
85	41.0
86	29.0
87	23.0
88	17.5
89	16.5
90	16.5
91	11.5
92	10.0
93	10.0
94	9.0
95	10.0
96	9.5
97	5.5
98	6.5
99	9.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06024718912986497
2	0.007345548996522727
3	0.007505234844273221
4	0.009330215961421725
5	0.003718399026190076
6	0.002189977340578204
7	7.299924468594013E-4
8	0.0013459235738970214
9	4.790575432514822E-4
10-11	3.9921461937623515E-4
12-13	2.395287716257411E-4
14-15	9.124905585742516E-5
16-17	4.790575432514822E-4
18-19	0.0015056094216475153
20-21	9.581150865029643E-4
22-23	0.0011178009342534584
24-25	0.0011862377261465274
26-27	0.003399027330689088
28-29	0.003706992894207898
30-31	0.004973073544229672
32-33	0.0058513457068573894
34-35	0.003239341482938594
36-37	0.00805272917941777
38-39	0.006307590986144515
40-41	0.005828533442893033
42-43	0.014291883373669217
44-45	0.019641359273310767
46-47	0.017998876267877117
48-49	0.021272436146762243
50-51	0.018078719191752364
52-53	0.02479693092925529
54-55	0.01878589937464741
56-57	0.015044688084492976
58-59	0.017325914480928604
60-61	0.015843117323245445
62-63	0.0109612928348732
64-65	0.011120978682623694
66-67	0.013687358378613776
68-69	0.005132759391980166
70-71	0.008406319270865294
72-73	0.00844053766681183
74-75	0.011189415474516762
76-77	0.01034536170783558
78-79	0.01206768763714448
80-81	0.010060208408281125
82-83	0.006193529666322733
84-85	0.010664733403336568
86-87	0.00928459143349301
88-89	0.00805272917941777
90-91	0.013151270175451403
92-93	0.008463349930776184
94-95	0.007402579656433618
96-97	0.009261779169528656
98-99	0.008942407474027667
100	0.25832607713237066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4383607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.53646978980952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44699046577225	48.04545951136547
2	15.186591661005771	19.60178027881344
3	5.1951667954940985	10.058331748512792
4	2.164313732794274	5.587086713285902
5	1.0581164277409159	3.414354943650139
6	0.5791578858184839	2.242608324099272
7	0.3602769204502357	1.627570041482157
8	0.23856422733107716	1.2316874440065049
9	0.1643757363494168	0.9547406768782648
>10	0.585653005579404	6.023570390463671
>50	0.015491459092781124	0.6795479124510875
>100	0.005301682571269774	0.5332620149912579
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.562452792871259E-5
2	2.2812263964356294E-5	0.0	0.0	0.0	4.562452792871259E-5
3	2.2812263964356294E-5	0.0	0.0	0.0	4.562452792871259E-5
4	2.2812263964356294E-5	0.0	0.0	0.0	4.562452792871259E-5
5	2.2812263964356294E-5	0.0	0.0	0.0	6.843679189306888E-5
6	2.2812263964356294E-5	0.0	0.0	0.0	6.843679189306888E-5
7	2.2812263964356294E-5	0.0	0.0	0.0	6.843679189306888E-5
8	6.843679189306888E-5	0.0	0.0	0.0	6.843679189306888E-5
9	6.843679189306888E-5	0.0	0.0	0.0	6.843679189306888E-5
10-11	6.843679189306888E-5	0.0	0.0	0.0	6.843679189306888E-5
12-13	1.0265518783960333E-4	0.0	0.0	1.1406131982178147E-5	1.8249811171485036E-4
14-15	1.3687358378613776E-4	0.0	0.0	2.2812263964356294E-5	2.0531037567920665E-4
16-17	1.3687358378613776E-4	0.0	0.0	2.2812263964356294E-5	2.2812263964356292E-4
18-19	1.3687358378613776E-4	0.0	0.0	2.2812263964356294E-5	2.737471675722755E-4
20-21	1.3687358378613776E-4	0.0	0.0	2.2812263964356294E-5	3.0796556351880995E-4
22-23	1.3687358378613776E-4	0.0	0.0	4.562452792871259E-5	3.193716955009881E-4
24-25	1.8249811171485036E-4	0.0	0.0	1.8249811171485036E-4	3.307778274831663E-4
26-27	1.8249811171485036E-4	0.0	0.0	5.018698072158385E-4	3.421839594653444E-4
28-29	2.395287716257411E-4	0.0	0.0	0.0026690348838296863	3.421839594653444E-4
30-31	2.737471675722755E-4	0.0	0.0	0.010333955575853402	3.5359009144752255E-4
32-33	2.737471675722755E-4	0.0	0.0	0.02394147103059193	3.87808487394057E-4
34-35	2.737471675722755E-4	0.0	0.0	0.045635934060694765	4.5624527928712585E-4
36-37	2.737471675722755E-4	0.0	0.0	0.08178196631221732	4.5624527928712585E-4
38-39	3.193716955009881E-4	0.0	0.0	0.13756935783705063	4.790575432514822E-4
40-41	3.193716955009881E-4	0.0	0.0	0.19869481912954332	4.790575432514822E-4
42-43	3.193716955009881E-4	0.0	0.0	0.26122323465584396	4.790575432514822E-4
44-45	3.421839594653444E-4	0.0	0.0	0.34073538070360776	4.904636752336603E-4
46-47	3.9921461937623515E-4	0.0	0.0	0.44113215441073983	5.132759391980166E-4
48-49	4.106207513584133E-4	0.0	0.0	0.5584670341114064	5.246820711801947E-4
50-51	4.2202688334059147E-4	0.0	0.0	0.6810145161279284	6.159311270376199E-4
52-53	4.334330153227696E-4	0.0	0.0	0.8004481241133159	7.071801828950451E-4
54-55	4.334330153227696E-4	0.0	0.0	0.9365574970566477	7.299924468594014E-4
56-57	4.790575432514822E-4	0.0	0.0	1.1078091626370703	7.413985788415796E-4
58-59	4.790575432514822E-4	0.0	0.0	1.3108383119198415	7.528047108237577E-4
60-61	4.904636752336603E-4	0.0	0.0	1.5286155898555687	7.528047108237577E-4
62-63	5.018698072158385E-4	0.0	0.0	1.7547535625342325	8.326476346990047E-4
64-65	5.018698072158385E-4	0.0	0.0	1.9899822224026926	8.896782946098955E-4
66-67	6.159311270376199E-4	0.0	0.0	2.265565777224099	8.896782946098955E-4
68-69	6.159311270376199E-4	0.0	0.0	2.582713276988562	8.896782946098955E-4
70-71	6.159311270376199E-4	0.0	0.0	2.9319462260188924	9.010844265920736E-4
72-73	6.159311270376199E-4	0.0	0.0	3.2970907291643616	0.001003739614431677
74-75	6.273372590197981E-4	0.0	0.0	3.6543535951101456	0.0011063948022712804
76-77	6.387433910019762E-4	0.0	0.0	4.0630124917676245	0.0011178009342534584
78-79	6.387433910019762E-4	0.0	0.0	4.517923253612835	0.0011292070662356365
80-81	7.984292387524703E-4	0.0	0.0	5.01647387642186	0.0011406131982178147
82-83	7.984292387524703E-4	0.0	0.0	5.541429694769627	0.0011406131982178147
84-85	7.984292387524703E-4	0.0	0.0	6.0556295306582	0.0011406131982178147
86-87	8.098353707346485E-4	0.0	0.0	6.601196229497763	0.0012090499901108837
88	8.212415027168266E-4	0.0	0.0	7.044221801817544	0.0012546745180395961
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6015	0.0	32.828766	1
GTACTAG	3975	0.0	32.05332	1
GTATCAA	9000	0.0	31.291414	1
GGGCACC	4775	0.0	30.005993	94
CAACGCA	8675	0.0	28.926085	5
TAGTACT	5365	0.0	28.906584	4
CTGTGCG	5635	0.0	28.352608	9
ATCAACG	8910	0.0	28.165415	3
TAGGCAT	4820	0.0	27.980326	5
CTAGTAC	6190	0.0	25.889061	3
GTACTGT	6145	0.0	25.617449	6
TATCAAC	10975	0.0	25.521383	2
GCATCGT	4410	0.0	25.253279	8
ACTGTGC	6755	0.0	24.834238	8
CTAGGCA	5670	0.0	24.533598	4
AACGCAG	10875	0.0	24.413616	6
GTACATG	18105	0.0	24.254326	1
TCTAACG	1920	0.0	24.2324	2
CTAACGC	1915	0.0	24.049711	3
TACATGG	18210	0.0	23.794876	2
>>END_MODULE