FastQCFastQC Report
Mon 16 Jan 2017
ERR1146686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383607
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64770.14775503369713572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61240.13970230451771792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77850.043.022371
GTATCAA127700.036.6157881
GGGCACC45050.034.34354894
TAGTACT54500.030.6390324
CAACGCA149200.030.2712975
ATCAACG150000.030.1666473
CTGTGCG55550.029.9550449
GTACTAG39900.029.9456521
TATCAAC168200.027.8272322
GTACTGT61800.027.5290746
CTAGTAC64350.027.0455843
AACGCAG173350.026.7315776
TAGGCAT49450.026.7064695
CTAACGC17550.026.2658923
ACTGTGC69950.025.4640778
TCTAACG18750.025.3392092
TCAACGC182600.024.9354824
GTGGTAT25300.024.914791
TAACGCC19100.024.8731734
ACGCAGA181550.024.7734897
Produced by FastQC (version 0.11.5)