##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146686_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4383607 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.096540588606594 33.0 31.0 34.0 30.0 34.0 2 32.19554353298551 33.0 31.0 34.0 30.0 34.0 3 32.35028117255949 34.0 31.0 34.0 30.0 34.0 4 35.946170585091224 37.0 35.0 37.0 35.0 37.0 5 35.849922677831294 37.0 35.0 37.0 35.0 37.0 6 35.88169423034501 37.0 35.0 37.0 35.0 37.0 7 35.80714306734158 37.0 35.0 37.0 35.0 37.0 8 35.81307197474591 37.0 35.0 37.0 35.0 37.0 9 37.53046566446308 39.0 37.0 39.0 35.0 39.0 10-11 37.47100253284567 39.0 37.0 39.0 34.0 39.0 12-13 37.447625437225554 39.0 37.0 39.0 34.0 39.0 14-15 38.67270857081851 40.0 38.0 41.0 34.0 41.0 16-17 38.6560220840965 40.0 38.0 41.0 34.0 41.0 18-19 38.70977621853419 40.0 38.0 41.0 34.0 41.0 20-21 38.70460273925103 40.0 38.0 41.0 34.0 41.0 22-23 38.666869885918146 40.0 38.0 41.0 34.0 41.0 24-25 38.59502813550576 40.0 38.0 41.0 34.0 41.0 26-27 38.46032376989999 40.0 38.0 41.0 34.0 41.0 28-29 38.399942672780654 40.0 38.0 41.0 34.0 41.0 30-31 38.28423693091101 40.0 38.0 41.0 34.0 41.0 32-33 38.16288800524317 40.0 37.0 41.0 33.0 41.0 34-35 38.07366296750598 40.0 37.0 41.0 33.0 41.0 36-37 38.065656433161095 40.0 37.0 41.0 33.0 41.0 38-39 37.98060535536146 40.0 37.0 41.0 33.0 41.0 40-41 37.87630392049287 40.0 37.0 41.0 33.0 41.0 42-43 37.7441256709372 40.0 37.0 41.0 33.0 41.0 44-45 37.56590234936663 40.0 36.5 41.0 32.0 41.0 46-47 37.34940335664214 40.0 36.0 41.0 31.5 41.0 48-49 37.1180836922653 39.5 36.0 41.0 31.0 41.0 50-51 36.922020838090646 39.0 35.0 41.0 31.0 41.0 52-53 36.73701873822174 39.0 35.0 41.0 31.0 41.0 54-55 36.53800933797213 39.0 35.0 41.0 31.0 41.0 56-57 36.328544506841055 38.0 35.0 40.5 30.0 41.0 58-59 36.05606672769708 38.0 35.0 40.0 30.0 41.0 60-61 35.86917828172096 37.0 35.0 40.0 30.0 41.0 62-63 35.64377897014947 37.0 35.0 40.0 30.0 41.0 64-65 35.375544956470776 36.0 34.0 39.5 30.0 41.0 66-67 35.10102947184818 36.0 34.0 39.0 29.0 41.0 68-69 34.81633663784186 35.0 34.0 39.0 29.0 41.0 70-71 34.557059175240845 35.0 34.0 38.0 29.0 40.0 72-73 34.29053767821796 35.0 34.0 37.0 29.0 40.0 74-75 34.015733846578854 35.0 34.0 37.0 29.0 39.0 76-77 33.76971202026094 35.0 34.0 36.0 29.0 39.0 78-79 33.546783504999425 35.0 33.5 36.0 29.0 38.5 80-81 33.4127952163595 35.0 33.0 35.5 29.0 37.0 82-83 33.27144278672792 35.0 33.0 35.0 29.0 37.0 84-85 33.156077974143216 35.0 33.0 35.0 29.0 36.5 86-87 33.029099552035575 35.0 33.0 35.0 29.0 36.0 88-89 32.937595455067026 35.0 33.0 35.0 29.0 36.0 90-91 32.89135716317635 35.0 33.0 35.0 29.0 36.0 92-93 32.844366294697494 35.0 33.0 35.0 29.0 35.5 94-95 32.79864196311394 35.0 33.0 35.0 29.0 35.0 96-97 32.718561563570816 35.0 33.0 35.0 29.0 35.0 98-99 32.63975979142291 35.0 33.0 35.0 28.0 35.0 100 31.404347835013496 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4419220799719028 1101 2 0.4772057732332655 1101 3 0.41385612829206764 1101 4 0.26342438580990546 1101 5 0.28789651920688897 1101 6 0.305808798283131 1101 7 0.3468888000235779 1101 8 0.30701440746317843 1101 9 0.4005086616338005 1101 10-11 0.40998174428648326 1101 12-13 0.4091701259473979 1101 14-15 0.5919418001510763 1101 16-17 0.603930216108644 1101 18-19 0.5509486968772705 1101 20-21 0.5637551347426779 1101 22-23 0.5721920562776006 1101 24-25 0.5217226146014511 1101 26-27 0.601258167592583 1101 28-29 0.6372434889731196 1101 30-31 0.6508140523270711 1101 32-33 0.6617059533974086 1101 34-35 0.673345494702879 1101 36-37 0.6067075219706979 1101 38-39 0.592472524339172 1101 40-41 0.5564108019327918 1101 42-43 0.5763309342125993 1101 44-45 0.6419618875745385 1101 46-47 0.6112481458641028 1101 48-49 0.5539703849062292 1101 50-51 0.6090035175429875 1101 52-53 0.6908569062094969 1101 54-55 0.7209983546741014 1101 56-57 0.6773708452742113 1101 58-59 0.6110783753472973 1101 60-61 0.6633868506727438 1101 62-63 0.6346386758722531 1101 64-65 0.6438515924697725 1101 66-67 0.6025754994283474 1101 68-69 0.5641511367754077 1101 70-71 0.5566500183148975 1101 72-73 0.6154591884876623 1101 74-75 0.5893343688740771 1101 76-77 0.5005445260228711 1101 78-79 0.5153477118700067 1101 80-81 0.4804948161931435 1101 82-83 0.5272843032807941 1101 84-85 0.5008935149025859 1101 86-87 0.4844765863957221 1101 88-89 0.4628236820314129 1101 90-91 0.46109156243026916 1101 92-93 0.4811302578322554 1101 94-95 0.5277924826737035 1101 96-97 0.5288749074995565 1101 98-99 0.5275435043600467 1101 100 0.8328871500794861 1102 1 0.32197006345531065 1102 2 0.36703065170736693 1102 3 0.2782205101475199 1102 4 0.21467738320470886 1102 5 0.2253919863657856 1102 6 0.1832623665470905 1102 7 0.24776168508857666 1102 8 0.2679448633280046 1102 9 0.2836034007011605 1102 10-11 0.29093254960504566 1102 12-13 0.29881121701855307 1102 14-15 0.4429955298470887 1102 16-17 0.3718513110417234 1102 18-19 0.3499522554292156 1102 20-21 0.37305257525750335 1102 22-23 0.4406377825262524 1102 24-25 0.41997654704844223 1102 26-27 0.5138732988968329 1102 28-29 0.45157793119859235 1102 30-31 0.47041532703634914 1102 32-33 0.5338861401516013 1102 34-35 0.4898932789128807 1102 36-37 0.5669105211843615 1102 38-39 0.5805994834702801 1102 40-41 0.5544817134130682 1102 42-43 0.4993038648290664 1102 44-45 0.5391113511035002 1102 46-47 0.5819883991403856 1102 48-49 0.5937580626250565 1102 50-51 0.5465275544701171 1102 52-53 0.5149950257959617 1102 54-55 0.46314471413634095 1102 56-57 0.4405164991575532 1102 58-59 0.4109918052317667 1102 60-61 0.41824873028551934 1102 62-63 0.4192235528500845 1102 64-65 0.4167310722037101 1102 66-67 0.4300671921748247 1102 68-69 0.4364165609612485 1102 70-71 0.37197690965643204 1102 72-73 0.34053747079654784 1102 74-75 0.4271098626591936 1102 76-77 0.3771395141247993 1102 78-79 0.3631985531996378 1102 80-81 0.3359953917066747 1102 82-83 0.31879314509499324 1102 84-85 0.3450147145514393 1102 86-87 0.3440294789603442 1102 88-89 0.3645285364783035 1102 90-91 0.3653959083066951 1102 92-93 0.3542943284917328 1102 94-95 0.35457749548233863 1102 96-97 0.3616263975316514 1102 98-99 0.35543976389960363 1102 100 0.6109263900126933 1103 1 0.3041138485140209 1103 2 0.38759369154206524 1103 3 0.29705312847189447 1103 4 0.24258431079166343 1103 5 0.2566800505747935 1103 6 0.2796656043002699 1103 7 0.2913129750551704 1103 8 0.2691342941714794 1103 9 0.2942574159219973 1103 10-11 0.3399534159475408 1103 12-13 0.3054875636823766 1103 14-15 0.49584131249787333 1103 16-17 0.46928109895224424 1103 18-19 0.43115546094754365 1103 20-21 0.3966681830033849 1103 22-23 0.41348536847925743 1103 24-25 0.4200765103218487 1103 26-27 0.4769550895517227 1103 28-29 0.5008807974582936 1103 30-31 0.4715462991813908 1103 32-33 0.46467715129552545 1103 34-35 0.5115311991659652 1103 36-37 0.4318563956956609 1103 38-39 0.4741830743448219 1103 40-41 0.45841133794748856 1103 42-43 0.46946989193629207 1103 44-45 0.4937959515630652 1103 46-47 0.47624811825679814 1103 48-49 0.47785108747848426 1103 50-51 0.5148896188041903 1103 52-53 0.4684002504843434 1103 54-55 0.4078727854088413 1103 56-57 0.41785824892878765 1103 58-59 0.47757774898634153 1103 60-61 0.4642834140184533 1103 62-63 0.4197415893952581 1103 64-65 0.421211199328809 1103 66-67 0.4329624390312645 1103 68-69 0.3917676566379953 1103 70-71 0.4332143779169897 1103 72-73 0.42737489484427726 1103 74-75 0.3437560706162657 1103 76-77 0.3852147411118878 1103 78-79 0.3542144640940421 1103 80-81 0.35298716653477413 1103 82-83 0.36657206684796506 1103 84-85 0.35684175695084264 1103 86-87 0.3160257257137431 1103 88-89 0.347926871627422 1103 90-91 0.36736886185592255 1103 92-93 0.36338151848448064 1103 94-95 0.3545114404558589 1103 96-97 0.33375414883359156 1103 98-99 0.36237488112966076 1103 100 0.6044368139362319 1104 1 0.23433620405329236 1104 2 0.3005369415302468 1104 3 0.2564151964628394 1104 4 0.21371434993770322 1104 5 0.18197278414690032 1104 6 0.1856818189410845 1104 7 0.20677618917494556 1104 8 0.21845241096371382 1104 9 0.24622742327848357 1104 10-11 0.26577626603653925 1104 12-13 0.3300317404178372 1104 14-15 0.37591919782179417 1104 16-17 0.3478841857640447 1104 18-19 0.3018587729678188 1104 20-21 0.3525838909379502 1104 22-23 0.3756425892919921 1104 24-25 0.3454362329767875 1104 26-27 0.3918852506117432 1104 28-29 0.42605265830935224 1104 30-31 0.39021133242921024 1104 32-33 0.3763017396537762 1104 34-35 0.46608546683730623 1104 36-37 0.383745295481539 1104 38-39 0.3146895441258195 1104 40-41 0.4404153440587777 1104 42-43 0.4339170006700286 1104 44-45 0.39073859495637464 1104 46-47 0.3760021872793544 1104 48-49 0.336975341660704 1104 50-51 0.42449112147292567 1104 52-53 0.4109978388568436 1104 54-55 0.35885192113067177 1104 56-57 0.3323122540501231 1104 58-59 0.33156550239717575 1104 60-61 0.3226855094738923 1104 62-63 0.38599959615509505 1104 64-65 0.32825475515856795 1104 66-67 0.29828963351103965 1104 68-69 0.3051784965795292 1104 70-71 0.3297169758726284 1104 72-73 0.2829685653300089 1104 74-75 0.3104448643685771 1104 76-77 0.2842179143221628 1104 78-79 0.2789753756461977 1104 80-81 0.2764822229353783 1104 82-83 0.22480120121052494 1104 84-85 0.31861335454403417 1104 86-87 0.34303998382196 1104 88-89 0.28784234484373883 1104 90-91 0.3034080600099358 1104 92-93 0.25482901817595405 1104 94-95 0.25475507862166324 1104 96-97 0.2531427513746394 1104 98-99 0.2617276952917891 1104 100 0.5062691602663065 1105 1 0.22084595031173393 1105 2 0.20718459020373814 1105 3 0.13440625652753369 1105 4 0.1642666497590639 1105 5 0.17151889535913512 1105 6 0.18760234546792276 1105 7 0.2524377148088064 1105 8 0.18088369511664126 1105 9 0.21884243266377013 1105 10-11 0.1935199560591201 1105 12-13 0.22532927426075844 1105 14-15 0.30169516629162985 1105 16-17 0.28180575262218355 1105 18-19 0.27836375153286497 1105 20-21 0.3335096259811223 1105 22-23 0.3171022922266573 1105 24-25 0.29341832783060084 1105 26-27 0.3466072721550475 1105 28-29 0.3136898579623093 1105 30-31 0.33633327449209816 1105 32-33 0.32887461109724825 1105 34-35 0.2524021867921036 1105 36-37 0.2914253583970918 1105 38-39 0.2541766080786374 1105 40-41 0.30114670738969096 1105 42-43 0.29753628406341903 1105 44-45 0.25567018036330325 1105 46-47 0.26689572078385737 1105 48-49 0.29138714718848746 1105 50-51 0.29714522196093895 1105 52-53 0.3345880961414096 1105 54-55 0.32966411494847847 1105 56-57 0.27227852146990017 1105 58-59 0.31107356721449264 1105 60-61 0.3006522307232018 1105 62-63 0.2782895442981044 1105 64-65 0.2732710624599264 1105 66-67 0.2549484582715422 1105 68-69 0.17208608940725867 1105 70-71 0.24361881597214108 1105 72-73 0.18199797300673737 1105 74-75 0.19605803790545906 1105 76-77 0.18277452348335288 1105 78-79 0.19267151968595897 1105 80-81 0.12239785180116769 1105 82-83 0.18028198206582147 1105 84-85 0.15026850045288143 1105 86-87 0.20507248660234012 1105 88-89 0.21501504758497703 1105 90-91 0.22868183603171133 1105 92-93 0.1798925126353197 1105 94-95 0.17786717739294744 1105 96-97 0.19789472227408567 1105 98-99 0.24371366493222268 1105 100 0.4237359889491472 1106 1 0.17147799937366415 1106 2 0.2279201139854905 1106 3 0.14868416323646727 1106 4 0.14661811936598212 1106 5 0.16882439565196705 1106 6 0.162939409621373 1106 7 0.1703229480950199 1106 8 0.11265712608619793 1106 9 0.14464508533651355 1106 10-11 0.17750889854747953 1106 12-13 0.15205555208044785 1106 14-15 0.2544513128067152 1106 16-17 0.1946294810703435 1106 18-19 0.20698608252644135 1106 20-21 0.20461153313007685 1106 22-23 0.25329493643666723 1106 24-25 0.22030084388765658 1106 26-27 0.21214876884762646 1106 28-29 0.2268111280852878 1106 30-31 0.2778227507656865 1106 32-33 0.288992566741868 1106 34-35 0.2791736902799542 1106 36-37 0.23162746681954616 1106 38-39 0.3462966435042176 1106 40-41 0.24968868178166304 1106 42-43 0.29085109872088566 1106 44-45 0.2371658323848962 1106 46-47 0.269202998615377 1106 48-49 0.37266270905053034 1106 50-51 0.3487221428564595 1106 52-53 0.25195269397294595 1106 54-55 0.31662920009957674 1106 56-57 0.3639593428758019 1106 58-59 0.30461816358611316 1106 60-61 0.2731913820928824 1106 62-63 0.32439273607785424 1106 64-65 0.28823666017810723 1106 66-67 0.3586118325942991 1106 68-69 0.29375703623286853 1106 70-71 0.29011935673090505 1106 72-73 0.22161042934862962 1106 74-75 0.2055753254183017 1106 76-77 0.20297271577516796 1106 78-79 0.17139679607674196 1106 80-81 0.19455886593763694 1106 82-83 0.11402243923444644 1106 84-85 0.10436155551388993 1106 86-87 0.11010454548910076 1106 88-89 0.19047268495960878 1106 90-91 0.17694990262344845 1106 92-93 0.16009658412120586 1106 94-95 0.15835387955421965 1106 96-97 0.061116957594499866 1106 98-99 0.18662763551049721 1106 100 0.37453949665406583 1107 1 0.10237829289746259 1107 2 0.17445738812958922 1107 3 0.14880608249881533 1107 4 0.1169522360223425 1107 5 0.052217279322022137 1107 6 0.035938264446116364 1107 7 0.09802019061633871 1107 8 0.12305750734387289 1107 9 0.0688055164046304 1107 10-11 0.13937660236074834 1107 12-13 0.024578058324514984 1107 14-15 0.16667511403473867 1107 16-17 0.16173857208295317 1107 18-19 0.07730247830507153 1107 20-21 0.14516944621486516 1107 22-23 0.09691033146566497 1107 24-25 0.07068098165407122 1107 26-27 0.16529838665120877 1107 28-29 0.15892501469867426 1107 30-31 0.1166005838016062 1107 32-33 0.1848663525899994 1107 34-35 0.14930296045977087 1107 36-37 0.03536782838661878 1107 38-39 0.161642380134424 1107 40-41 0.16611367444933478 1107 42-43 0.22169613802090993 1107 44-45 0.2654983208473851 1107 46-47 0.31540351726396665 1107 48-49 0.3161932687888793 1107 50-51 0.1964046816606384 1107 52-53 0.25576970562971724 1107 54-55 0.2870549208948674 1107 56-57 0.2052343445003686 1107 58-59 0.18679407797705494 1107 60-61 0.22939986290217718 1107 62-63 0.2908145548920942 1107 64-65 0.20409994666498932 1107 66-67 0.1869177138599909 1107 68-69 0.1386649236656723 1107 70-71 0.14212512387432952 1107 72-73 0.15722596563645652 1107 74-75 0.1620237744862365 1107 76-77 0.15586960167676267 1107 78-79 0.1565989103101515 1107 80-81 0.0650102975337461 1107 82-83 0.21178607716971243 1107 84-85 0.1531682336779454 1107 86-87 0.12752384832455022 1107 88-89 0.12646695442489886 1107 90-91 0.08521611566199994 1107 92-93 0.030406109883500676 1107 94-95 0.04637196642501351 1107 96-97 0.0765357093228829 1107 98-99 0.15602299502150885 1107 100 0.1456975588370284 1108 1 0.049809206734458655 1108 2 0.0948214513284995 1108 3 0.086475340744947 1108 4 0.057874044482765896 1108 5 0.07907929886311393 1108 6 0.12333215589573143 1108 7 0.09355819241027774 1108 8 0.047986794546929445 1108 9 0.08334259789393172 1108 10-11 0.054900408118825794 1108 12-13 0.09336893165458093 1108 14-15 0.11371252436962465 1108 16-17 0.13079946919222607 1108 18-19 0.05057593249585324 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-0.3956233063017507 2215 7 -0.4718269449506636 2215 8 -0.3887300886482876 2215 9 -0.4877312715844795 2215 10-11 -0.5079079408332348 2215 12-13 -0.5248604855402732 2215 14-15 -0.7109276835516809 2215 16-17 -0.6553725914981641 2215 18-19 -0.6326756156004691 2215 20-21 -0.6010988944450872 2215 22-23 -0.6051814346927316 2215 24-25 -0.6016772289488799 2215 26-27 -0.654808167458377 2215 28-29 -0.6383504381064355 2215 30-31 -0.6638025317822596 2215 32-33 -0.6854438902640112 2215 34-35 -0.6225679153284744 2215 36-37 -0.6861236300521867 2215 38-39 -0.8077167478561478 2215 40-41 -0.872507022975995 2215 42-43 -0.8093167793284479 2215 44-45 -0.8294725947715946 2215 46-47 -0.8764602037438536 2215 48-49 -0.7647550026430849 2215 50-51 -0.7753131651516796 2215 52-53 -0.818735311956921 2215 54-55 -0.8190695330536286 2215 56-57 -0.8882642969262236 2215 58-59 -0.8865728577780914 2215 60-61 -0.8190219596725612 2215 62-63 -0.8578365051615435 2215 64-65 -0.8231848980559278 2215 66-67 -0.8840185662021227 2215 68-69 -0.7893001074767056 2215 70-71 -0.800978160613262 2215 72-73 -0.7528075946364297 2215 74-75 -0.7768661370545118 2215 76-77 -0.6867162925686543 2215 78-79 -0.7323803285872188 2215 80-81 -0.7753884321300006 2215 82-83 -0.6989813202458777 2215 84-85 -0.6729960737025067 2215 86-87 -0.6337309082224394 2215 88-89 -0.6535616436223393 2215 90-91 -0.653021192688044 2215 92-93 -0.6280498646702171 2215 94-95 -0.6142482137032914 2215 96-97 -0.6230654807682896 2215 98-99 -0.6994349587485722 2215 100 -0.23799259758898827 2216 1 -0.40077159030561305 2216 2 -0.24157809824856358 2216 3 -0.2668089379931047 2216 4 -0.38586008095983004 2216 5 -0.42183007010846296 2216 6 -0.41963576309253625 2216 7 -0.4443765835261999 2216 8 -0.4246782181015618 2216 9 -0.5099714394801254 2216 10-11 -0.5455629724875024 2216 12-13 -0.49673525178120315 2216 14-15 -0.7466420539272036 2216 16-17 -0.6365156088577066 2216 18-19 -0.5771219806007082 2216 20-21 -0.6746140172746209 2216 22-23 -0.6418934624730568 2216 24-25 -0.698264043514591 2216 26-27 -0.771144595103415 2216 28-29 -0.6474620377191442 2216 30-31 -0.6987405257053183 2216 32-33 -0.6556245616674943 2216 34-35 -0.6694863912245523 2216 36-37 -0.8307872790537729 2216 38-39 -0.9891670300000612 2216 40-41 -1.0417183712088018 2216 42-43 -0.9854507215679646 2216 44-45 -0.9891875627801809 2216 46-47 -0.9492976227907874 2216 48-49 -0.9773808440440632 2216 50-51 -0.9416130934567803 2216 52-53 -0.9698622019155252 2216 54-55 -0.9257617854744424 2216 56-57 -0.855373406971836 2216 58-59 -0.871364788493743 2216 60-61 -0.8708546440116791 2216 62-63 -0.9738711531295863 2216 64-65 -0.8208916227317218 2216 66-67 -0.9455762866520772 2216 68-69 -0.8523829577518427 2216 70-71 -0.8656285795095258 2216 72-73 -0.7968342402740021 2216 74-75 -0.7399184076914196 2216 76-77 -0.8321085784927504 2216 78-79 -0.8346532174859362 2216 80-81 -0.8796924898715233 2216 82-83 -0.8080199870263272 2216 84-85 -0.8032948652141698 2216 86-87 -0.8295789387462378 2216 88-89 -0.7758770687715071 2216 90-91 -0.7894535590094591 2216 92-93 -0.8030386178954387 2216 94-95 -0.7996417151745945 2216 96-97 -0.8430151257705596 2216 98-99 -0.8791570040601684 2216 100 -0.24071427757794694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 11.0 13 56.0 14 228.0 15 544.0 16 1354.0 17 2826.0 18 5024.0 19 7768.0 20 11125.0 21 16049.0 22 22435.0 23 27047.0 24 28429.0 25 30028.0 26 34369.0 27 42406.0 28 53718.0 29 67355.0 30 84623.0 31 105883.0 32 133608.0 33 170612.0 34 237956.0 35 350760.0 36 585431.0 37 1102308.0 38 991998.0 39 269332.0 40 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.09271360320394 20.54725708759932 13.523497886557806 25.836531422638938 2 14.681837698191258 20.477584522591147 38.63101167029333 26.20956610892426 3 17.57260630343915 25.595998911398766 29.4398653893928 27.391529395769282 4 12.320629305244871 16.86271187872558 37.02698389177126 33.789674924258286 5 13.341483746245512 37.97956298030441 34.47987392486868 14.199079348581403 6 31.666935471177048 37.24829803401628 16.851989697069104 14.23277679773757 7 27.36010033748471 31.830894473636544 22.56783570421247 18.24116948466628 8 26.91110311667994 34.273145380048895 19.273990574428776 19.54176092884239 9 26.315452092306636 14.476822397628256 20.53589658014507 38.671828929920046 10-11 26.113574962354065 25.30889516327536 26.8830326258718 21.69449724849878 12-13 26.294963634895353 23.070819906387403 27.242062342395446 23.392154116321798 14-15 23.61683519220764 24.15334267133772 24.911482311805162 27.318339824649478 16-17 22.820271069007784 26.726483464416408 26.070938841004683 24.382306625571136 18-19 22.40361410465318 26.526786298850514 28.23138865449266 22.838210942003645 20-21 23.757216374551824 25.86916436623995 27.54876292514361 22.82485633406462 22-23 24.038902209983696 25.379978177788292 27.5032638646667 23.077855747561312 24-25 23.593036510800353 25.46533026341093 27.976002410800056 22.96563081498866 26-27 23.450368611967267 25.702737494487987 27.186561204049543 23.660332689495203 28-29 22.7931244748902 26.02752710268051 27.83745212561254 23.341896296816753 30-31 23.440080280919343 25.779055923580742 27.717014778012718 23.063849017487197 32-33 23.651835121168478 25.932536835532932 27.0724086351719 23.343219408126686 34-35 23.64517394009089 25.92278459268817 27.58667690785237 22.845364559368576 36-37 23.55872686579796 25.37844975610268 27.23515132629362 23.82767205180574 38-39 23.518314940185103 25.542971803813618 27.759046374367045 23.179666881634233 40-41 23.225280003430964 25.016327877932486 28.17759438745307 23.580797731183477 42-43 23.401755677459224 25.160421543263347 27.489382602044387 23.948440177233042 44-45 23.617092043150766 25.73354545697185 27.262480418522923 23.386882081354464 46-47 23.642436468415166 25.708007127463755 26.65910744279768 23.990448961323406 48-49 24.23272661075685 25.677780877710983 26.627603706262903 23.46188880526927 50-51 23.656203669717655 25.623818467303295 26.638063129290558 24.08191473368849 52-53 23.37676484228627 25.989647338367693 26.40651865005234 24.22706916929369 54-55 23.373263159767742 25.9509235202882 26.331603175193397 24.344210144750658 56-57 24.11703421406162 25.608899246670607 26.076664719259735 24.197401820008043 58-59 23.183011522589904 25.890679284283387 26.849878062732135 24.076431130394575 60-61 23.370400220640217 26.25855830597953 26.330530998787072 24.040510474593184 62-63 23.69091252933942 25.79867447059009 26.014010836281624 24.49640216378886 64-65 23.04046555289539 25.859971416220212 26.428179546286675 24.671383484597722 66-67 23.603734188520264 25.67564854755323 26.32657428147576 24.394042982450735 68-69 23.221150983653416 25.458600645541445 26.714746554606744 24.605501816198398 70-71 23.098251741751028 25.376657473671987 26.59576057668695 24.92933020789004 72-73 23.532984578407582 25.378228458201193 26.79351691785325 24.295270045537976 74-75 23.572206391085114 25.759614179289787 26.482126923123868 24.186052506501234 76-77 23.40278538187791 25.74697737019801 26.028389907838307 24.821847340085775 78-79 23.36488231779016 25.76853100295385 26.050559054513673 24.816027624742322 80-81 23.080604625369016 26.175236511849715 26.40178510527974 24.34237375750153 82-83 23.626102887416685 25.377468828752214 27.156186674581 23.8402416092501 84-85 23.7238876568999 25.741176159267926 26.169111418975287 24.365824764856885 86-87 22.71960054813308 26.14620790595507 26.552882135647653 24.581309410264197 88-89 23.43266629513093 25.21903765551976 26.40437429723969 24.94392175210962 90-91 23.13203487447666 25.403634495519327 26.34522209678012 25.119108533223894 92-93 23.335691361018448 25.51309914415229 26.290803441093146 24.86040605373611 94-95 22.512989873407903 25.479622146784596 26.860083488323657 25.14730449148384 96-97 22.890886432109447 25.349101778512534 26.491722455959216 25.268289333418803 98-99 22.405224738440285 25.782169797611875 26.354232941046043 25.4583725229018 100 22.95782720955231 25.197117488437513 25.785234216048664 26.05982108596151 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 237.0 1 167.5 2 124.0 3 153.0 4 111.0 5 85.5 6 179.0 7 412.5 8 688.5 9 802.5 10 653.0 11 495.5 12 495.0 13 541.5 14 667.0 15 855.0 16 1027.0 17 1275.0 18 1616.0 19 2029.0 20 2598.5 21 3198.5 22 3870.5 23 4724.0 24 5598.5 25 6624.0 26 8214.5 27 10113.5 28 12451.0 29 16942.0 30 24469.5 31 31984.5 32 42762.5 33 54369.0 34 62400.5 35 71660.0 36 82271.0 37 97728.5 38 108127.0 39 111739.5 40 111431.5 41 108876.5 42 111665.0 43 120676.5 44 134607.5 45 158789.5 46 183030.5 47 198044.0 48 208089.5 49 207442.5 50 200439.0 51 204953.0 52 210003.5 53 205197.0 54 200522.0 55 181305.5 56 160813.5 57 136924.0 58 113811.0 59 106956.5 60 89871.5 61 67146.0 62 51668.5 63 41443.5 64 30436.0 65 17961.5 66 12177.0 67 9256.5 68 6833.5 69 5107.5 70 3882.5 71 2942.0 72 2153.5 73 1710.0 74 1256.5 75 868.5 76 531.0 77 238.5 78 114.5 79 53.5 80 18.5 81 7.5 82 4.0 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007778982011845495 3 0.0 4 0.08942407474027668 5 0.00431151788926334 6 0.0 7 2.281226396435629E-5 8 0.0 9 0.0 10-11 0.0 12-13 2.0531037567920663E-4 14-15 0.010117239068192016 16-17 0.0 18-19 9.124905585742516E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.1406131982178146E-5 60-61 0.0 62-63 0.0 64-65 4.562452792871258E-5 66-67 9.124905585742516E-5 68-69 0.0 70-71 3.5359009144752255E-4 72-73 2.9655943153663185E-4 74-75 3.764023554118788E-4 76-77 1.5968584775049406E-4 78-79 1.1406131982178146E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.06487807871462929 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4383607.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.095641526939126 #Duplication Level Percentage of deduplicated Percentage of total 1 72.72222629872115 45.157332952863335 2 16.278381683279346 20.216331072872123 3 5.434931729070681 10.124567171152814 4 2.2524276160420857 5.5946375124450975 5 1.1845538794363508 3.6777816533412353 6 0.6323799804112132 2.3560824343456495 7 0.39757597884693363 1.7281414823540786 8 0.25726987903307763 1.2780270547293586 9 0.177476270465356 0.9918452583319356 >10 0.6344682811459995 6.32511963596009 >50 0.019220023327185413 0.817076736395719 >100 0.008282767487012038 0.8889678689028948 >500 5.907826714294666E-4 0.24855774634129157 >1k 1.0741503106542397E-4 0.17528773832855377 >5k 1.0741503106542397E-4 0.42024368163586484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6477 0.14775503369713572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6124 0.13970230451771792 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.2812263964356294E-5 0.0 0.0 0.0 0.0 7 6.843679189306888E-5 0.0 0.0 0.0 0.0 8 6.843679189306888E-5 0.0 0.0 0.0 0.0 9 6.843679189306888E-5 0.0 0.0 0.0 0.0 10-11 6.843679189306888E-5 0.0 0.0 0.0 0.0 12-13 6.843679189306888E-5 0.0 0.0 1.1406131982178147E-5 1.5968584775049406E-4 14-15 6.843679189306888E-5 0.0 0.0 2.2812263964356294E-5 2.1671650766138482E-4 16-17 6.843679189306888E-5 0.0 0.0 2.2812263964356294E-5 2.5093490360791925E-4 18-19 6.843679189306888E-5 0.0 0.0 2.2812263964356294E-5 2.5093490360791925E-4 20-21 6.843679189306888E-5 0.0 0.0 2.2812263964356294E-5 2.851532995544537E-4 22-23 6.843679189306888E-5 0.0 0.0 4.562452792871259E-5 2.9655943153663185E-4 24-25 1.3687358378613776E-4 0.0 0.0 1.8249811171485036E-4 2.9655943153663185E-4 26-27 1.3687358378613776E-4 0.0 0.0 5.018698072158385E-4 3.193716955009881E-4 28-29 1.7109197973267222E-4 0.0 0.0 0.002703253279776221 3.193716955009881E-4 30-31 1.8249811171485036E-4 0.0 0.0 0.01034536170783558 3.193716955009881E-4 32-33 1.939042436970285E-4 0.0 0.0 0.024089750746360244 3.421839594653444E-4 34-35 2.167165076613848E-4 0.0 0.0 0.045898275096284864 3.421839594653444E-4 36-37 2.5093490360791925E-4 0.0 0.0 0.0822039931955579 3.421839594653444E-4 38-39 2.737471675722755E-4 0.0 0.0 0.13812825830417735 3.764023554118788E-4 40-41 2.737471675722755E-4 0.0 0.0 0.1995844974241532 4.106207513584133E-4 42-43 3.0796556351880995E-4 0.0 0.0 0.262318223326133 4.106207513584133E-4 44-45 3.9921461937623515E-4 0.0 0.0 0.34197864908966524 4.2202688334059147E-4 46-47 4.5624527928712585E-4 0.0 0.0 0.442614951568423 4.334330153227696E-4 48-49 5.018698072158385E-4 0.0 0.0 0.5601437355127866 4.5624527928712585E-4 50-51 5.018698072158385E-4 0.0 0.0 0.6832387118644532 4.790575432514822E-4 52-53 5.018698072158385E-4 0.0 0.0 0.8035049674845396 5.246820711801947E-4 54-55 5.018698072158385E-4 0.0 0.0 0.9400477734431942 5.246820711801947E-4 56-57 5.246820711801947E-4 0.0 0.0 1.11215489892228 5.360882031623729E-4 58-59 5.246820711801947E-4 0.0 0.0 1.3161079448956077 5.703065991089074E-4 60-61 5.246820711801947E-4 0.0 0.0 1.5355961426286617 5.703065991089074E-4 62-63 5.246820711801947E-4 0.0 0.0 1.7630572266172584 5.931188630732637E-4 64-65 5.246820711801947E-4 0.0 0.0 1.9994721242118647 6.159311270376199E-4 66-67 5.47494335144551E-4 0.0 0.0 2.277165813449974 6.159311270376199E-4 68-69 5.47494335144551E-4 0.0 0.0 2.596469072159069 6.159311270376199E-4 70-71 5.817127310910855E-4 0.0 0.0 2.948234182489443 6.159311270376199E-4 72-73 6.159311270376199E-4 0.0 0.0 3.315215072884043 6.729617869485106E-4 74-75 6.273372590197981E-4 0.0 0.0 3.675158379845638 7.071801828950451E-4 76-77 6.387433910019762E-4 0.0 0.0 4.087478644869396 7.299924468594014E-4 78-79 6.387433910019762E-4 0.0 0.0 4.545925307629083 7.642108428059359E-4 80-81 6.387433910019762E-4 0.0 0.0 5.048011831352582 7.870231067702921E-4 82-83 7.071801828950451E-4 0.0 0.0 5.577781037396829 8.896782946098955E-4 84-85 7.299924468594014E-4 0.0 0.0 6.096600356738184 8.896782946098955E-4 86-87 7.528047108237577E-4 0.0 0.0 6.647653405061175 9.35302822538608E-4 88 7.528047108237577E-4 0.0 0.0 7.09488784008238 9.809273504673207E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7785 0.0 43.02237 1 GTATCAA 12770 0.0 36.615788 1 GGGCACC 4505 0.0 34.343548 94 TAGTACT 5450 0.0 30.639032 4 CAACGCA 14920 0.0 30.271297 5 ATCAACG 15000 0.0 30.166647 3 CTGTGCG 5555 0.0 29.955044 9 GTACTAG 3990 0.0 29.945652 1 TATCAAC 16820 0.0 27.827232 2 GTACTGT 6180 0.0 27.529074 6 CTAGTAC 6435 0.0 27.045584 3 AACGCAG 17335 0.0 26.731577 6 TAGGCAT 4945 0.0 26.706469 5 CTAACGC 1755 0.0 26.265892 3 ACTGTGC 6995 0.0 25.464077 8 TCTAACG 1875 0.0 25.339209 2 TCAACGC 18260 0.0 24.935482 4 GTGGTAT 2530 0.0 24.91479 1 TAACGCC 1910 0.0 24.873173 4 ACGCAGA 18155 0.0 24.773489 7 >>END_MODULE