FastQCFastQC Report
Mon 16 Jan 2017
ERR1146685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889200
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC106010.15387853451779598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC79550.042.9367494
GTACTAG91500.037.103961
CAACGCA125250.033.5430225
TACCCCG3800.033.3904765
TAGGCAT113050.032.7565465
GCATCGT96950.032.5238088
ATCAACG135000.031.0885963
GTATCAA154950.030.8930171
CTGTGCG118300.030.4275889
TATTCTC34400.030.0543025
CTAGGCA127500.029.6364464
ACTGTGC125600.029.5946718
TAGTACT122700.029.4552044
GTACTGT128050.028.7348446
TCTAACG44800.028.4300652
GTGGTAT20000.027.7431761
TACTGTG145450.027.5265987
AGGGCTA80550.027.4788025
GGTATCA103050.027.423991
TAACGCC46450.027.4197734
Produced by FastQC (version 0.11.5)