FastQCFastQC Report
Mon 16 Jan 2017
ERR1146685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889200
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144760.21012599430993437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119750.17382279509957616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75510.10960634035882251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC79400.045.1299194
GTATCAA234200.040.011991
GTGGTAT26700.039.9946861
GGTATCA133300.039.3488851
CAACGCA265150.033.837755
ATCAACG270000.033.2407463
GTACTAG85400.032.6100541
CTGTGCG119800.031.3499669
TAGTACT123950.030.7393474
TATCAAC306250.030.5524732
CAGTACG9900.030.387289
ACTGTGC127900.030.205678
TAGGCAT111450.029.8566025
AACGCAG318350.029.4516416
GTACTGT131550.029.1878876
CTAACGC42850.028.2122973
TCTAACG43650.028.128332
TACTGTG147250.028.0871897
ACGCAGA322100.027.810777
TAACGCC43650.027.8029964
Produced by FastQC (version 0.11.5)