FastQCFastQC Report
Mon 16 Jan 2017
ERR1146684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6213233
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC76850.040.4677694
GTACTAG79450.034.267041
CTGTGCG105000.030.9243769
TAGTACT107050.030.9077974
CTAACGC39850.030.5464323
TAGGCAT100750.030.4120855
GCATCGT84450.029.9917148
TATTCGC6000.029.7607969
TCTAACG41350.029.6656652
TAACGCC42050.029.1713564
GTACTGT115450.029.061636
ACTGTGC114700.028.9646438
ACGTTAA9000.028.7165578
CGCCTAC42400.028.2609357
CAACGCA127950.028.0605855
CTAGGCA113050.027.8123464
CTAGTAC127300.027.5791763
TACTGTG128300.027.0663667
GTACATG255600.026.159571
TACATGG253900.025.93372
Produced by FastQC (version 0.11.5)