##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146684_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6213233
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649698796101806	33.0	31.0	34.0	30.0	34.0
2	31.6660365062762	33.0	31.0	34.0	28.0	34.0
3	31.814315832031408	33.0	31.0	34.0	30.0	34.0
4	35.26464644091087	37.0	35.0	37.0	32.0	37.0
5	35.40254067407419	37.0	35.0	37.0	33.0	37.0
6	35.48581020541158	37.0	35.0	37.0	33.0	37.0
7	35.41050947228279	37.0	35.0	37.0	33.0	37.0
8	35.39408034432316	37.0	35.0	37.0	33.0	37.0
9	36.96654881605116	39.0	37.0	39.0	33.0	39.0
10-11	36.815836055077924	39.0	37.0	39.0	32.0	39.0
12-13	36.77132074396695	39.0	37.0	39.0	32.0	39.0
14-15	37.98911041964787	40.0	38.0	41.0	32.0	41.0
16-17	38.014034319974805	40.0	38.0	41.0	32.0	41.0
18-19	37.97058753470215	40.0	38.0	41.0	32.0	41.0
20-21	38.02383412951035	40.0	38.0	41.0	32.0	41.0
22-23	37.942781238044674	40.0	38.0	41.0	32.0	41.0
24-25	37.88574241461732	40.0	38.0	41.0	32.0	41.0
26-27	37.913713359856295	40.0	38.0	41.0	32.0	41.0
28-29	37.886996592595196	40.0	37.0	41.0	32.0	41.0
30-31	37.739737830530416	40.0	37.0	41.0	32.0	41.0
32-33	37.73888432962356	40.0	37.0	41.0	32.0	41.0
34-35	37.67555868257315	40.0	37.0	41.0	31.5	41.0
36-37	37.55275771888806	40.0	37.0	41.0	31.0	41.0
38-39	37.4416079358363	40.0	37.0	41.0	31.0	41.0
40-41	37.34785481246237	40.0	36.0	41.0	31.0	41.0
42-43	37.17679266172699	40.0	36.0	41.0	30.5	41.0
44-45	36.99890668835371	39.0	35.5	41.0	30.0	41.0
46-47	36.982166369746636	39.0	35.0	41.0	30.0	41.0
48-49	36.817883700804394	39.0	35.0	41.0	30.0	41.0
50-51	36.61314922521013	39.0	35.0	41.0	30.0	41.0
52-53	36.37211979656968	38.5	35.0	40.5	30.0	41.0
54-55	36.127629770201764	38.0	35.0	40.0	29.0	41.0
56-57	35.85414026803759	37.5	34.5	40.0	29.0	41.0
58-59	35.511255975753684	37.0	34.0	40.0	28.0	41.0
60-61	35.238859704762405	36.5	34.0	40.0	28.0	41.0
62-63	34.9062673168703	36.0	34.0	39.0	28.0	41.0
64-65	34.73347965543864	35.5	34.0	39.0	28.0	41.0
66-67	34.46850037653505	35.0	33.5	39.0	28.0	41.0
68-69	34.327214752770416	35.0	33.0	38.0	28.0	41.0
70-71	34.25679569718373	35.0	34.0	37.5	28.0	40.0
72-73	34.0389863055192	35.0	33.5	37.0	28.0	40.0
74-75	33.79526866286843	35.0	33.0	36.5	28.0	39.0
76-77	33.55610565385203	35.0	33.0	36.0	27.0	39.0
78-79	33.321274367145094	35.0	33.0	36.0	27.0	39.0
80-81	33.17131620526705	35.0	33.0	35.5	27.0	37.5
82-83	32.93053149624358	35.0	33.0	35.0	26.0	37.0
84-85	32.78714471193982	35.0	33.0	35.0	26.0	37.0
86-87	32.64457328415014	35.0	33.0	35.0	26.0	36.0
88-89	32.54699123628552	35.0	33.0	35.0	26.0	36.0
90-91	32.42154857221675	35.0	33.0	35.0	26.0	36.0
92-93	32.28516860706817	35.0	33.0	35.0	25.0	36.0
94-95	32.24859111190583	35.0	33.0	35.0	25.0	35.0
96-97	32.155082064361665	35.0	33.0	35.0	25.0	35.0
98-99	32.081702794664224	35.0	32.0	35.0	25.0	35.0
100	31.137167719285596	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3597994949257348
1101	2	0.42901379286690045
1101	3	0.4797217177648392
1101	4	0.5073261485325489
1101	5	0.41835138181044584
1101	6	0.4198179037413894
1101	7	0.38691396577236503
1101	8	0.41323211353550704
1101	9	0.572068109998952
1101	10-11	0.5876483914626078
1101	12-13	0.5153117426268636
1101	14-15	0.6692870034735776
1101	16-17	0.6518687973725505
1101	18-19	0.6854261499844085
1101	20-21	0.676972388266158
1101	22-23	0.6785174873889019
1101	24-25	0.7049347979284519
1101	26-27	0.6477887096001851
1101	28-29	0.6364711150489057
1101	30-31	0.6568912611084556
1101	32-33	0.6468609470830557
1101	34-35	0.6646866528939626
1101	36-37	0.6615891825124436
1101	38-39	0.6294763772308514
1101	40-41	0.6530832059847711
1101	42-43	0.6049171303305414
1101	44-45	0.6041002817219763
1101	46-47	0.5839018247998666
1101	48-49	0.5282975338333031
1101	50-51	0.5088028268004408
1101	52-53	0.49602249923923125
1101	54-55	0.5599180107813098
1101	56-57	0.4731941194253224
1101	58-59	0.46560287157060287
1101	60-61	0.4348608009055255
1101	62-63	0.4528678550628129
1101	64-65	0.4939643252709942
1101	66-67	0.5152813159632146
1101	68-69	0.4955394844667538
1101	70-71	0.5026595268723923
1101	72-73	0.490880066631064
1101	74-75	0.4570291102380821
1101	76-77	0.48390994857884806
1101	78-79	0.4941260129371372
1101	80-81	0.4755942260477113
1101	82-83	0.42923182067785604
1101	84-85	0.42953405841174686
1101	86-87	0.4895971131461536
1101	88-89	0.5008258194312205
1101	90-91	0.4536305654538566
1101	92-93	0.4649717903075157
1101	94-95	0.5352895894075971
1101	96-97	0.5364739476707427
1101	98-99	0.49873736338130215
1101	100	0.6679236791057228
1102	1	0.27993219875708775
1102	2	0.32704378780642784
1102	3	0.36562473828487896
1102	4	0.45565517971932223
1102	5	0.3663113906629789
1102	6	0.32941295811053806
1102	7	0.35087490724642123
1102	8	0.3724605534391827
1102	9	0.4846190272127373
1102	10-11	0.6234454920728396
1102	12-13	0.5519192160725481
1102	14-15	0.7152763362202492
1102	16-17	0.6389105819217136
1102	18-19	0.6425359022212973
1102	20-21	0.6285573580072779
1102	22-23	0.637010395754892
1102	24-25	0.7127248849301395
1102	26-27	0.614538686771489
1102	28-29	0.6358605821625503
1102	30-31	0.653528047395767
1102	32-33	0.6070427845948103
1102	34-35	0.6423843803658542
1102	36-37	0.6919887360962065
1102	38-39	0.6438033147559992
1102	40-41	0.5972501110260566
1102	42-43	0.6884657683583981
1102	44-45	0.6425346038779693
1102	46-47	0.5815697052253626
1102	48-49	0.5426538657781421
1102	50-51	0.5530540072482282
1102	52-53	0.5132141558555716
1102	54-55	0.5094954627088057
1102	56-57	0.5398667684835843
1102	58-59	0.5173298924106362
1102	60-61	0.49528274108985215
1102	62-63	0.5354118591932959
1102	64-65	0.5309187160262567
1102	66-67	0.549150634741622
1102	68-69	0.4133720025895826
1102	70-71	0.39413110573167387
1102	72-73	0.46717784868131673
1102	74-75	0.3912393463908046
1102	76-77	0.44899723316341067
1102	78-79	0.41767028522262706
1102	80-81	0.3924843727592844
1102	82-83	0.3970515363072451
1102	84-85	0.41328435215858406
1102	86-87	0.4202815715506816
1102	88-89	0.40031879858592134
1102	90-91	0.4071909744073352
1102	92-93	0.3982632074264316
1102	94-95	0.44500628720112445
1102	96-97	0.4902498016681136
1102	98-99	0.4199828709955611
1102	100	0.5340884915380109
1103	1	0.2624901162558082
1103	2	0.299950338875572
1103	3	0.29537596008644584
1103	4	0.3871904811219906
1103	5	0.2526971227967749
1103	6	0.24780845120781692
1103	7	0.2664567707743686
1103	8	0.2676769175297693
1103	9	0.3584486143307828
1103	10-11	0.45862487001379293
1103	12-13	0.3801028308441232
1103	14-15	0.5103055365132363
1103	16-17	0.48761222228682044
1103	18-19	0.5094386779144031
1103	20-21	0.5115844125427813
1103	22-23	0.5132349220217023
1103	24-25	0.5151498941192685
1103	26-27	0.4517727048939477
1103	28-29	0.48764690115694975
1103	30-31	0.5022463912242188
1103	32-33	0.5157480612778755
1103	34-35	0.49649157993284376
1103	36-37	0.5025436501434726
1103	38-39	0.5558243335873243
1103	40-41	0.5329383451014067
1103	42-43	0.5187832267827588
1103	44-45	0.43614523415743633
1103	46-47	0.39333402487675073
1103	48-49	0.4457199377769854
1103	50-51	0.4202813680921409
1103	52-53	0.42967606288738125
1103	54-55	0.4073551800072934
1103	56-57	0.3746552485876862
1103	58-59	0.4310426491748487
1103	60-61	0.4262281748780907
1103	62-63	0.38835950979058964
1103	64-65	0.3154873930168023
1103	66-67	0.3685714210566786
1103	68-69	0.39444963036536507
1103	70-71	0.3724806195803154
1103	72-73	0.3191193899853033
1103	74-75	0.32534598515788105
1103	76-77	0.31047810144914223
1103	78-79	0.3521293778509076
1103	80-81	0.2633754381382829
1103	82-83	0.3139938154056807
1103	84-85	0.28981066997809535
1103	86-87	0.31944531992204617
1103	88-89	0.39133609723305796
1103	90-91	0.3540488581946235
1103	92-93	0.33426536257287154
1103	94-95	0.3332495231231718
1103	96-97	0.33856165437718744
1103	98-99	0.3336053732314639
1103	100	0.47157082221735536
1104	1	0.1553991604900382
1104	2	0.25713711348924306
1104	3	0.23148877709353144
1104	4	0.3463404364907703
1104	5	0.19008589125220254
1104	6	0.1954206903878557
1104	7	0.17892799707639995
1104	8	0.2543340962200915
1104	9	0.28115452879639236
1104	10-11	0.40632182019842844
1104	12-13	0.272492456079803
1104	14-15	0.4432869474117638
1104	16-17	0.4166765550729039
1104	18-19	0.4035069219764722
1104	20-21	0.35582376122991377
1104	22-23	0.4068550487375049
1104	24-25	0.3995664345037824
1104	26-27	0.3665937267871655
1104	28-29	0.3688948373738228
1104	30-31	0.32750667126565247
1104	32-33	0.3669611112595632
1104	34-35	0.3769065890724761
1104	36-37	0.37406464848007204
1104	38-39	0.3540788871906315
1104	40-41	0.40831356521141515
1104	42-43	0.43729891205030214
1104	44-45	0.3611500789306419
1104	46-47	0.34354671138761717
1104	48-49	0.32410452599575734
1104	50-51	0.276777122678304
1104	52-53	0.3233319693653982
1104	54-55	0.391525052777034
1104	56-57	0.38255316370416637
1104	58-59	0.37150035420803107
1104	60-61	0.36999128525988567
1104	62-63	0.416547902423126
1104	64-65	0.33215728093652785
1104	66-67	0.28282770291919945
1104	68-69	0.31081279224422786
1104	70-71	0.31347321594167
1104	72-73	0.33722311158342677
1104	74-75	0.24814711891862373
1104	76-77	0.22601942475365888
1104	78-79	0.27987063305138093
1104	80-81	0.3110936498825225
1104	82-83	0.3088973589688635
1104	84-85	0.3429042269003446
1104	86-87	0.28409136910780575
1104	88-89	0.2737411703004895
1104	90-91	0.33541785249706635
1104	92-93	0.33394321577682007
1104	94-95	0.3593519233843523
1104	96-97	0.31136220147875804
1104	98-99	0.29138944423605295
1104	100	0.3472921712343009
1105	1	0.19196367488709143
1105	2	0.27564426065566394
1105	3	0.25997930546476056
1105	4	0.31340754709670193
1105	5	0.1866064806096901
1105	6	0.17585692508329487
1105	7	0.23301523398735213
1105	8	0.25050236230976
1105	9	0.3324231909088482
1105	10-11	0.36003233053762784
1105	12-13	0.3208807920991319
1105	14-15	0.43218110157489775
1105	16-17	0.40465939126455197
1105	18-19	0.4245874254495803
1105	20-21	0.3787071279801495
1105	22-23	0.3863565627020549
1105	24-25	0.38233980283968805
1105	26-27	0.35306912125298595
1105	28-29	0.4472142193122792
1105	30-31	0.40643033332490575
1105	32-33	0.394589872436363
1105	34-35	0.4051475031354812
1105	36-37	0.42398225216274454
1105	38-39	0.39948094376114796
1105	40-41	0.3627734856774225
1105	42-43	0.38082188812624196
1105	44-45	0.3582367643623243
1105	46-47	0.4126362855116241
1105	48-49	0.26800403919794036
1105	50-51	0.3319312837898849
1105	52-53	0.36800297614166766
1105	54-55	0.3845614329620801
1105	56-57	0.36940870837372586
1105	58-59	0.40593126410073666
1105	60-61	0.43488177702163
1105	62-63	0.3562177780849396
1105	64-65	0.36070313420238875
1105	66-67	0.36088122020873925
1105	68-69	0.34894839411680323
1105	70-71	0.35155691882041396
1105	72-73	0.3493783437314235
1105	74-75	0.3240608889048673
1105	76-77	0.325569437221894
1105	78-79	0.3754275029024754
1105	80-81	0.2793273931494724
1105	82-83	0.35171947968812844
1105	84-85	0.2888278745980415
1105	86-87	0.2999854501472399
1105	88-89	0.3005896542112012
1105	90-91	0.28573862684866214
1105	92-93	0.3175380515966566
1105	94-95	0.264621143714443
1105	96-97	0.2586972579116349
1105	98-99	0.25809421478475514
1105	100	0.40159694150167624
1106	1	0.07840047815786733
1106	2	0.1766990093689671
1106	3	0.12038491698971399
1106	4	0.18664395414624835
1106	5	0.12902580205762604
1106	6	0.1755407743335553
1106	7	0.15251782687839466
1106	8	0.11127393074426095
1106	9	0.17788700090832776
1106	10-11	0.2445371839692001
1106	12-13	0.18339984655263208
1106	14-15	0.28209933684935606
1106	16-17	0.2322109937881791
1106	18-19	0.24091712690969302
1106	20-21	0.20091315559309209
1106	22-23	0.24396887489616148
1106	24-25	0.2777349727097942
1106	26-27	0.271308004685217
1106	28-29	0.24642160278536096
1106	30-31	0.3002919097989434
1106	32-33	0.23720303407382914
1106	34-35	0.21593848576041808
1106	36-37	0.2567420216897318
1106	38-39	0.2779495273537975
1106	40-41	0.2536075624671099
1106	42-43	0.17160731919786087
1106	44-45	0.21537801843779647
1106	46-47	0.2002967281876593
1106	48-49	0.12039249521627227
1106	50-51	0.1639139751049541
1106	52-53	0.14883384779124498
1106	54-55	0.22564524842028533
1106	56-57	0.21450871988530906
1106	58-59	0.18552850494160822
1106	60-61	0.17714347566698052
1106	62-63	0.22601658533648816
1106	64-65	0.20937719025264556
1106	66-67	0.16714515711412048
1106	68-69	0.15258336184724186
1106	70-71	0.1858547030265214
1106	72-73	0.0815970456891364
1106	74-75	0.07992014495958699
1106	76-77	0.09258835984920921
1106	78-79	0.09631509462379029
1106	80-81	0.09256903147041129
1106	82-83	0.14723191219682974
1106	84-85	0.12827815941686538
1106	86-87	0.117478680130084
1106	88-89	0.20293105431667158
1106	90-91	0.14160099288726968
1106	92-93	0.21719530352206107
1106	94-95	0.17343539505313288
1106	96-97	0.17902631741537078
1106	98-99	0.1457267372323372
1106	100	0.15335860811882895
1107	1	0.025818999889796856
1107	2	0.07780393222409643
1107	3	0.05528020727574656
1107	4	0.10630192139119998
1107	5	0.04121501824393192
1107	6	0.13641918412167797
1107	7	0.05945353682196952
1107	8	0.06880857676995333
1107	9	0.13601121986123843
1107	10-11	0.23440248628146776
1107	12-13	0.1484760903936575
1107	14-15	0.2059622193271764
1107	16-17	0.13146976362960316
1107	18-19	0.10468882802085488
1107	20-21	0.1471911368404335
1107	22-23	0.2043491789587364
1107	24-25	0.2248041817161166
1107	26-27	0.1863406851541498
1107	28-29	0.19464108007665004
1107	30-31	0.24362839594336094
1107	32-33	0.27056351977219606
1107	34-35	0.19620087876637626
1107	36-37	0.20241496786523072
1107	38-39	0.23089594517070822
1107	40-41	0.15995772822788012
1107	42-43	0.19745580805994223
1107	44-45	0.2062128719114753
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2216	84-85	-0.8888614506310653
2216	86-87	-0.8884045289780467
2216	88-89	-0.9410708574944664
2216	90-91	-1.031379806087152
2216	92-93	-0.9625425280153301
2216	94-95	-0.962595747218149
2216	96-97	-0.9642158627349602
2216	98-99	-1.0086205004477193
2216	100	-0.606553779827351
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	18.0
3	25.0
4	53.0
5	40.0
6	74.0
7	287.0
8	659.0
9	1057.0
10	1515.0
11	2223.0
12	3824.0
13	5699.0
14	8241.0
15	11245.0
16	13967.0
17	16467.0
18	19241.0
19	21910.0
20	25432.0
21	29563.0
22	34540.0
23	40520.0
24	48064.0
25	56226.0
26	63700.0
27	72882.0
28	84726.0
29	103392.0
30	121656.0
31	145440.0
32	182276.0
33	238928.0
34	352390.0
35	519648.0
36	872003.0
37	1541047.0
38	1172442.0
39	401475.0
40	338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66364324590519	20.438889061559674	11.432495285953292	24.46497240658184
2	16.279743174139718	19.85790557191076	39.22026728818224	24.642083965767284
3	19.030430919591293	26.561077524320915	28.365412473683193	26.043079082404603
4	12.345838781892178	16.089570122388956	40.12764597147885	31.43694512424002
5	13.233084328398087	38.69088278827155	34.854311832809806	13.221721050520555
6	30.073224493146324	38.328897801977604	17.89231919408796	13.70555851078812
7	27.08140966760827	31.507982786917037	23.75575409761924	17.654853447855455
8	26.884579783938868	34.187478845268316	19.714334872703038	19.213606498089778
9	26.60114482162559	14.471266393538269	21.251369062133087	37.67621972270305
10-11	26.34496167231124	25.26978258778994	27.10224485862553	21.28301088127329
12-13	26.24300191149042	23.215873200579185	27.164087124305787	23.37703776362461
14-15	23.603614101420554	24.33727578848835	24.547368207585826	27.51174190250527
16-17	22.64303449006232	26.941362138049573	25.853403453987568	24.56219991790054
18-19	21.890794413029365	28.03385312196284	27.41519598521487	22.660156479792928
20-21	23.768356664811737	27.13068887110409	26.2633863778931	22.837568086191073
22-23	24.399968936889174	26.663673019716626	25.957220624135154	22.97913741925905
24-25	23.84482948429396	26.61689268184651	26.887504120291922	22.650773713567606
26-27	23.73306898464286	26.79637682628988	25.639102875484106	23.831451313583152
28-29	22.628467038737483	27.49079949621558	26.39161593278582	23.489117532261115
30-31	23.478662068279515	27.141450623001838	26.416499662074234	22.963387646644414
32-33	23.934840864524194	27.23505682012976	25.335767749781862	23.49433456556418
34-35	23.865594314898203	27.01121082837291	26.526470015906938	22.596724840821945
36-37	23.50306214286502	26.459856702160216	25.98169289496098	24.055388260013782
38-39	23.765082862185867	26.61978101501309	26.67303358235501	22.942102540446037
40-41	23.226445709528754	25.63956568073681	27.444019643805927	23.68996896592851
42-43	23.26480616404073	25.94899823392214	26.644105168838188	24.14209043319895
44-45	23.495959040665547	26.842683090752356	26.575354373741856	23.08600349484024
46-47	23.412098617433553	26.669737551988206	26.035178244287987	23.882985586290253
48-49	24.270059494416184	26.353140792451295	26.186622429189654	23.190177283942866
50-51	23.546077975747696	25.969159276130128	26.45895252032683	24.02581022779535
52-53	23.1294931252447	26.705077440808587	26.145014450122606	24.020414983824107
54-55	23.03145830635297	26.554602206877746	26.177562602024008	24.23637688474528
56-57	24.341251907099352	25.94804448068787	25.767990800207457	23.94271281200532
58-59	22.986009909881922	26.478614683437367	26.907779439208408	23.627595967472303
60-61	23.013830009940843	27.20289898115039	26.046096169802524	23.73717483910624
62-63	23.553912033038998	26.435601107888772	25.53291030701306	24.477576552059165
64-65	22.616714779048433	26.740761988095684	26.148950718691783	24.4935725141641
66-67	23.54153128530747	26.20073372739537	26.061270470989502	24.196464516307657
68-69	22.76610682693083	26.201277050734205	26.573164036363643	24.45945208597132
70-71	22.780162325976985	26.022472734811558	26.452464023600754	24.74490091561071
72-73	23.50574773697645	25.987830423651143	27.044688739496124	23.461733099876287
74-75	23.64302363230337	26.298171865283003	26.47084696270097	23.587957539712654
76-77	23.387764579194386	26.221000864777942	26.027023405341826	24.364211150685847
78-79	23.347842579017012	26.244876136734863	25.934009719635913	24.473271564612205
80-81	22.831393836533707	26.701885906852645	26.793618718580024	23.673101538033624
82-83	23.798654221354198	25.499166282314455	27.998548810530842	22.703630685800498
84-85	23.93903130679266	26.0739383753781	26.54597364003647	23.441056677792773
86-87	22.433470266212293	26.84524787270884	27.043745954325964	23.677535906752905
88-89	23.858501196977624	25.35137928875401	26.729324028354036	24.060795485914326
90-91	23.333222533953908	25.58368670421129	26.70550225157485	24.377588510259958
92-93	23.807889289797806	25.757447842427638	26.79615274450023	23.63851012327433
94-95	22.508002460091518	25.839089347645988	27.578488653302703	24.074419538959795
96-97	22.915274682168793	25.688188282847925	27.079534298793124	24.31700273619016
98-99	22.223873867855936	26.39497838204222	26.83895897070803	24.542188779393815
100	23.25386900487791	25.51218024536373	25.85795818521847	25.37599256453989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	310.0
1	217.5
2	150.0
3	184.5
4	190.0
5	181.5
6	179.5
7	171.5
8	177.5
9	184.5
10	170.0
11	177.0
12	182.0
13	180.5
14	214.0
15	272.5
16	308.0
17	365.5
18	464.0
19	552.5
20	695.5
21	938.0
22	1309.0
23	1952.0
24	2810.0
25	3946.5
26	6127.0
27	8716.0
28	12688.5
29	22066.0
30	37062.5
31	51178.5
32	71349.0
33	92720.0
34	107561.5
35	124024.5
36	138285.5
37	164474.0
38	178518.5
39	170775.0
40	159162.0
41	146297.5
42	146555.5
43	158852.5
44	180714.5
45	222327.0
46	260981.0
47	292093.5
48	315675.0
49	310434.0
50	293326.5
51	304513.0
52	316285.5
53	305118.0
54	289507.0
55	247827.0
56	216613.5
57	178987.0
58	138108.5
59	135531.5
60	114169.0
61	78137.0
62	58825.5
63	49809.5
64	36742.0
65	17816.0
66	9828.5
67	6855.0
68	4891.0
69	3624.5
70	2800.0
71	2187.5
72	1680.5
73	1417.5
74	1117.0
75	846.0
76	570.5
77	310.5
78	197.5
79	129.5
80	84.0
81	69.5
82	53.5
83	34.5
84	25.5
85	25.5
86	26.5
87	24.5
88	19.5
89	18.0
90	12.0
91	12.0
92	13.0
93	12.5
94	10.0
95	7.0
96	7.5
97	5.5
98	7.0
99	9.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05794084979591141
2	0.007451837070974161
3	0.007612783875962804
4	0.009351009369840145
5	0.003814439278230834
6	0.002382012713831913
7	8.369233859409424E-4
8	0.0013519531619045995
9	6.115978589568426E-4
10-11	5.71361157709682E-4
12-13	2.9775158922898913E-4
14-15	2.414202074829642E-4
16-17	6.035505187074105E-4
18-19	0.0015853260291381315
20-21	9.73728170181289E-4
22-23	0.001215148377664253
24-25	0.0013036691204080066
26-27	0.0036534924732421915
28-29	0.004152427568706984
30-31	0.005311244564625213
32-33	0.006148167950566154
34-35	0.0035730190707478696
36-37	0.008184145033672485
38-39	0.006228641353060476
40-41	0.006180357311563882
42-43	0.014155271498751135
44-45	0.019603320847616693
46-47	0.01813065758197061
48-49	0.021011605391267316
50-51	0.01805823151972572
52-53	0.024480009038772566
54-55	0.018565213955439946
56-57	0.014791011378456273
58-59	0.016899414523807492
60-61	0.01579692890963529
62-63	0.011201897627209538
64-65	0.011185802946710673
66-67	0.013583910341041451
68-69	0.0052146764816320265
70-71	0.008835979593876489
72-73	0.008481896622901476
74-75	0.011242134328456698
76-77	0.010550063067005535
78-79	0.012191720477889691
80-81	0.010059175311790174
82-83	0.0064137301787974155
84-85	0.010743199232991907
86-87	0.009310772668592985
88-89	0.008079529610429868
90-91	0.012932075780837448
92-93	0.0087635535316316
94-95	0.0073391743074821105
96-97	0.009334914689341282
98-99	0.009238346606348096
100	0.2559537039734386
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6213233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.24772792747455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36043910569354	45.76633365269495
2	15.61397301196199	19.750966338550043
3	5.794740099419462	10.99512435655527
4	2.67511874067141	6.767807291346947
5	1.3440162782047251	4.250298794699471
6	0.7589866368886466	2.8802508186313225
7	0.4520741816183153	2.0014865389421646
8	0.27652472767981684	1.3991648593209636
9	0.18248667871578553	1.0387681025224018
>10	0.5370848704393144	4.809196276349565
>50	0.0035995628226654186	0.14845159199862826
>100	8.796876129500838E-4	0.07586272617487042
>500	3.8209183095187286E-5	0.012421550246020293
>1k	3.820908823309131E-5	0.10386710196736895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	8.047340249432139E-6	0.0
22-23	0.0	0.0	0.0	6.437872199545713E-5	0.0
24-25	0.0	0.0	0.0	1.3680478424034636E-4	0.0
26-27	0.0	0.0	0.0	5.230771162130891E-4	0.0
28-29	0.0	0.0	0.0	0.0026475749420631738	0.0
30-31	0.0	0.0	0.0	0.010582252428003263	0.0
32-33	0.0	8.047340249432139E-6	0.0	0.026870069092853912	0.0
34-35	0.0	1.6094680498864278E-5	0.0	0.05214676481632026	0.0
36-37	0.0	1.6094680498864278E-5	0.0	0.0974291483998749	0.0
38-39	0.0	1.6094680498864278E-5	0.0	0.16471296022537701	0.0
40-41	0.0	1.6094680498864278E-5	0.0	0.23903014742888284	0.0
42-43	0.0	1.6094680498864278E-5	0.0	0.31642946594792115	0.0
44-45	0.0	1.6094680498864278E-5	0.0	0.41138003355097097	0.0
46-47	0.0	1.6094680498864278E-5	0.0	0.5322591314376911	0.0
48-49	0.0	1.6094680498864278E-5	0.0	0.6690236789767903	0.0
50-51	0.0	1.6094680498864278E-5	0.0	0.8156381709811945	0.0
52-53	0.0	1.6094680498864278E-5	0.0	0.9630010656287958	0.0
54-55	0.0	1.6094680498864278E-5	0.0	1.1224510653310442	0.0
56-57	0.0	1.6094680498864278E-5	0.0	1.3191280610271656	0.0
58-59	0.0	1.6094680498864278E-5	0.0	1.558093829734053	0.0
60-61	0.0	1.6094680498864278E-5	0.0	1.8085994843586262	0.0
62-63	0.0	1.6094680498864278E-5	0.0	2.0735823040919277	0.0
64-65	0.0	1.6094680498864278E-5	0.0	2.3497589741121896	0.0
66-67	0.0	1.6094680498864278E-5	0.0	2.666035540595371	0.0
68-69	0.0	1.6094680498864278E-5	0.0	3.0205289259231063	0.0
70-71	0.0	1.6094680498864278E-5	0.0	3.4117503721492497	0.0
72-73	0.0	1.6094680498864278E-5	0.0	3.8234040152687014	0.0
74-75	0.0	1.6094680498864278E-5	0.0	4.230406295723981	0.0
76-77	0.0	1.6094680498864278E-5	0.0	4.6959207871328825	0.0
78-79	0.0	1.6094680498864278E-5	0.0	5.197777067108219	0.0
80-81	0.0	1.6094680498864278E-5	0.0	5.742606144015523	0.0
82-83	0.0	1.6094680498864278E-5	0.0	6.311070259235409	0.0
84-85	0.0	1.6094680498864278E-5	0.0	6.8624740131265	0.0
86-87	0.0	1.6094680498864278E-5	0.0	7.454146657625748	0.0
88	0.0	1.6094680498864278E-5	0.0	7.929044347765487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	7685	0.0	40.46776	94
GTACTAG	7945	0.0	34.26704	1
CTGTGCG	10500	0.0	30.924376	9
TAGTACT	10705	0.0	30.907797	4
CTAACGC	3985	0.0	30.546432	3
TAGGCAT	10075	0.0	30.412085	5
GCATCGT	8445	0.0	29.991714	8
TATTCGC	600	0.0	29.760796	9
TCTAACG	4135	0.0	29.665665	2
TAACGCC	4205	0.0	29.171356	4
GTACTGT	11545	0.0	29.06163	6
ACTGTGC	11470	0.0	28.964643	8
ACGTTAA	900	0.0	28.716557	8
CGCCTAC	4240	0.0	28.260935	7
CAACGCA	12795	0.0	28.060585	5
CTAGGCA	11305	0.0	27.812346	4
CTAGTAC	12730	0.0	27.579176	3
TACTGTG	12830	0.0	27.066366	7
GTACATG	25560	0.0	26.15957	1
TACATGG	25390	0.0	25.9337	2
>>END_MODULE