FastQCFastQC Report
Mon 16 Jan 2017
ERR1146684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6213233
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84630.1362092810618884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72810.11718536871223081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC74000.041.2414994
GTACTAG77800.033.4416161
GTATCAA210650.031.8269581
CAGTACG10850.031.1924369
CTAACGC38650.030.7938373
TAGGCAT96100.030.5177045
CTGTGCG105150.030.4427879
TAACGCC39600.030.0550984
TCTAACG39700.029.9827732
TAGTACT107950.029.8075454
ACTGTGC112100.029.3473638
CAACGCA218450.028.7438565
CGCCTAC41150.028.5522167
GTACTGT115550.028.226646
TACCCCG5000.028.1995335
ATCAACG224950.027.8764153
CTAGGCA109700.027.6167144
GGTATCA140000.027.4559061
TACTGTG129750.027.0934267
TATTCTC29050.026.694915
Produced by FastQC (version 0.11.5)