##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6213233
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.115132009374186	33.0	31.0	34.0	30.0	34.0
2	32.20436317131516	33.0	31.0	34.0	30.0	34.0
3	32.36321541458368	34.0	31.0	34.0	30.0	34.0
4	35.96465736919893	37.0	35.0	37.0	35.0	37.0
5	35.86618576834315	37.0	35.0	37.0	35.0	37.0
6	35.90056110884623	37.0	35.0	37.0	35.0	37.0
7	35.82496696969195	37.0	35.0	37.0	35.0	37.0
8	35.82384098584424	37.0	35.0	37.0	35.0	37.0
9	37.54628902537536	39.0	37.0	39.0	35.0	39.0
10-11	37.48435701670934	39.0	37.0	39.0	34.5	39.0
12-13	37.44817287553839	39.0	37.0	39.0	34.0	39.0
14-15	38.671903580631856	40.0	38.0	41.0	34.0	41.0
16-17	38.669174808026675	40.0	38.0	41.0	34.0	41.0
18-19	38.71147299642553	40.0	38.0	41.0	34.0	41.0
20-21	38.703668846798436	40.0	38.0	41.0	34.0	41.0
22-23	38.65984142233198	40.0	38.0	41.0	34.0	41.0
24-25	38.58273188853532	40.0	38.0	41.0	34.0	41.0
26-27	38.44350928091704	40.0	38.0	41.0	34.0	41.0
28-29	38.38740636316069	40.0	38.0	41.0	34.0	41.0
30-31	38.27275123916969	40.0	38.0	41.0	34.0	41.0
32-33	38.15210664077784	40.0	37.0	41.0	33.0	41.0
34-35	38.059116083365936	40.0	37.0	41.0	33.0	41.0
36-37	38.050347460009945	40.0	37.0	41.0	33.0	41.0
38-39	37.95227854806025	40.0	37.0	41.0	33.0	41.0
40-41	37.84019865342246	40.0	37.0	41.0	33.0	41.0
42-43	37.69969185124717	40.0	37.0	41.0	33.0	41.0
44-45	37.49037811715736	40.0	36.0	41.0	32.0	41.0
46-47	37.26450005979174	40.0	36.0	41.0	31.5	41.0
48-49	37.01281748165569	39.0	35.0	41.0	31.0	41.0
50-51	36.794119180787206	39.0	35.0	41.0	31.0	41.0
52-53	36.58338130889345	39.0	35.0	41.0	31.0	41.0
54-55	36.37083849905516	38.0	35.0	40.5	30.0	41.0
56-57	36.12601112818399	38.0	35.0	40.0	30.0	41.0
58-59	35.820690532609994	37.0	35.0	40.0	30.0	41.0
60-61	35.628135867430046	37.0	35.0	40.0	30.0	41.0
62-63	35.39353071098412	36.0	34.5	39.5	30.0	41.0
64-65	35.12922080984248	36.0	34.0	39.0	29.0	41.0
66-67	34.87319636974824	35.0	34.0	39.0	29.0	41.0
68-69	34.61494820812289	35.0	34.0	38.5	29.0	41.0
70-71	34.41215346342234	35.0	34.0	37.0	29.0	40.0
72-73	34.18385468563629	35.0	34.0	37.0	29.0	40.0
74-75	33.94077769174277	35.0	34.0	36.0	29.0	39.0
76-77	33.72409693954822	35.0	34.0	36.0	29.0	39.0
78-79	33.51984739667738	35.0	33.0	36.0	29.0	39.0
80-81	33.41480844191744	35.0	33.0	35.0	29.0	37.5
82-83	33.273646344825636	35.0	33.0	35.0	29.0	37.0
84-85	33.17070983817925	35.0	33.0	35.0	29.0	37.0
86-87	33.05645112616894	35.0	33.0	35.0	29.0	36.0
88-89	32.97560247619879	35.0	33.0	35.0	29.0	36.0
90-91	32.92590081524385	35.0	33.0	35.0	29.0	36.0
92-93	32.869240136334824	35.0	33.0	35.0	29.0	36.0
94-95	32.83717510674394	35.0	33.0	35.0	29.0	35.0
96-97	32.75610378686909	35.0	33.0	35.0	29.0	35.0
98-99	32.68823791092335	35.0	33.0	35.0	29.0	35.0
100	31.442256229566798	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3689584654323852
1101	2	0.4428353460397858
1101	3	0.37164146551435095
1101	4	0.2661539707654299
1101	5	0.26763400206552035
1101	6	0.29305087032559385
1101	7	0.32477201377174936
1101	8	0.3203906711835671
1101	9	0.37232288436649696
1101	10-11	0.36579278976804375
1101	12-13	0.35472556302358527
1101	14-15	0.5337471423339792
1101	16-17	0.5414913266241896
1101	18-19	0.5256596412494403
1101	20-21	0.5464354881895161
1101	22-23	0.5251684347563526
1101	24-25	0.4933353299496588
1101	26-27	0.5487061976457568
1101	28-29	0.603623159096621
1101	30-31	0.6049339278660568
1101	32-33	0.6088976433564781
1101	34-35	0.6188005361334277
1101	36-37	0.5167058345214244
1101	38-39	0.5303199428259617
1101	40-41	0.5619884452370414
1101	42-43	0.5419130944040376
1101	44-45	0.5023931577090437
1101	46-47	0.5452862047524647
1101	48-49	0.4326320163265507
1101	50-51	0.4235620341769675
1101	52-53	0.4780698282205904
1101	54-55	0.5142785806292665
1101	56-57	0.5382305531323794
1101	58-59	0.44045569454791433
1101	60-61	0.4766728574663688
1101	62-63	0.49333730144540056
1101	64-65	0.4628468914109831
1101	66-67	0.43012149110335685
1101	68-69	0.40219354052327105
1101	70-71	0.42625382799140255
1101	72-73	0.48567221078906186
1101	74-75	0.4893764605328741
1101	76-77	0.40852474639547864
1101	78-79	0.389721161137885
1101	80-81	0.44603363737878254
1101	82-83	0.4026036129729249
1101	84-85	0.3984274653525546
1101	86-87	0.36802871880075827
1101	88-89	0.40574570396739773
1101	90-91	0.40945155097020347
1101	92-93	0.3821812896039347
1101	94-95	0.41809640276124327
1101	96-97	0.3884851202701398
1101	98-99	0.3818789831548344
1101	100	0.78795902812778
1102	1	0.3215773977018017
1102	2	0.37402790422533627
1102	3	0.28782779087241295
1102	4	0.24298522074405327
1102	5	0.23434401914165193
1102	6	0.19062058075766686
1102	7	0.26780265475693454
1102	8	0.24230569788986145
1102	9	0.32612180390447065
1102	10-11	0.28076782556370716
1102	12-13	0.33483496184322803
1102	14-15	0.4354997286876596
1102	16-17	0.42397937083213577
1102	18-19	0.40967856367685584
1102	20-21	0.4102263525512413
1102	22-23	0.45197646130132796
1102	24-25	0.4480998969635408
1102	26-27	0.45088612149394436
1102	28-29	0.4802117537986277
1102	30-31	0.5360261581867292
1102	32-33	0.5583049129096196
1102	34-35	0.5465465394002322
1102	36-37	0.5089438393219083
1102	38-39	0.5731201652446174
1102	40-41	0.5382966087542229
1102	42-43	0.5256781906227346
1102	44-45	0.5433434264035171
1102	46-47	0.5660336782665638
1102	48-49	0.5998564489050437
1102	50-51	0.5995232803845738
1102	52-53	0.5649071544494362
1102	54-55	0.586108766145685
1102	56-57	0.5259763432435918
1102	58-59	0.519758746804456
1102	60-61	0.5318909853760445
1102	62-63	0.6473081928121047
1102	64-65	0.5666693633264188
1102	66-67	0.57926486876692
1102	68-69	0.5821006915250564
1102	70-71	0.5404697009254065
1102	72-73	0.5216008889270114
1102	74-75	0.5566665897709555
1102	76-77	0.4540427421223683
1102	78-79	0.4975182612217992
1102	80-81	0.4600070655602124
1102	82-83	0.458780625273576
1102	84-85	0.45540982422710385
1102	86-87	0.4924061926227594
1102	88-89	0.48612162622718813
1102	90-91	0.4754562467090082
1102	92-93	0.44600361415253786
1102	94-95	0.4540587515885335
1102	96-97	0.4555194101633262
1102	98-99	0.4670405906216999
1102	100	0.7469608652132251
1103	1	0.2869449582691459
1103	2	0.3405490885687712
1103	3	0.26172656445884
1103	4	0.24526271545039435
1103	5	0.212759909115249
1103	6	0.22019169951161643
1103	7	0.21413417961629477
1103	8	0.18699983926904196
1103	9	0.2653950402109615
1103	10-11	0.31640924807200577
1103	12-13	0.3101928365587483
1103	14-15	0.40846235974767353
1103	16-17	0.3616956895639021
1103	18-19	0.31905357121022604
1103	20-21	0.3690073308780981
1103	22-23	0.35759428073112076
1103	24-25	0.3641504027048228
1103	26-27	0.3912317861236758
1103	28-29	0.4102347542075506
1103	30-31	0.35268111614639963
1103	32-33	0.3746569056968738
1103	34-35	0.38003922280115177
1103	36-37	0.3665441308363526
1103	38-39	0.3359300785629671
1103	40-41	0.35044244362858734
1103	42-43	0.3215937719660644
1103	44-45	0.379130675919761
1103	46-47	0.30781837195625883
1103	48-49	0.30928526531653233
1103	50-51	0.31334013404585903
1103	52-53	0.2624761883472573
1103	54-55	0.2255470831673918
1103	56-57	0.17553926822873933
1103	58-59	0.24366617980123806
1103	60-61	0.30188076156232313
1103	62-63	0.3110496747119029
1103	64-65	0.27051716756005106
1103	66-67	0.2778528963993807
1103	68-69	0.2866417116911464
1103	70-71	0.3386690891459878
1103	72-73	0.30759323007504236
1103	74-75	0.2075683928187928
1103	76-77	0.2601163573874956
1103	78-79	0.23697613011363217
1103	80-81	0.24487938017556132
1103	82-83	0.2914669494572877
1103	84-85	0.2673385711729992
1103	86-87	0.30570229535521065
1103	88-89	0.3106787751001505
1103	90-91	0.3293817699435664
1103	92-93	0.22063404764706718
1103	94-95	0.2217231430400659
1103	96-97	0.2436353178617523
1103	98-99	0.27225525581845034
1103	100	0.5372873332306902
1104	1	0.2079157799052922
1104	2	0.226018778855277
1104	3	0.16805321292918762
1104	4	0.16509967319842644
1104	5	0.14376311820534937
1104	6	0.1429945739094336
1104	7	0.18749495398576244
1104	8	0.18632537463420817
1104	9	0.21496530270290037
1104	10-11	0.2245290750758926
1104	12-13	0.23996554416872584
1104	14-15	0.2600294037371782
1104	16-17	0.25533509222366746
1104	18-19	0.24503697636311017
1104	20-21	0.31962539242470456
1104	22-23	0.2907699983023022
1104	24-25	0.35136219994355145
1104	26-27	0.3510854101494161
1104	28-29	0.33631789141836066
1104	30-31	0.2980123471339766
1104	32-33	0.297333100396024
1104	34-35	0.3584117426028328
1104	36-37	0.3220734321407619
1104	38-39	0.3154136405665895
1104	40-41	0.3634642140989044
1104	42-43	0.4166392612597889
1104	44-45	0.3748423908328036
1104	46-47	0.355283417707291
1104	48-49	0.34122730958003444
1104	50-51	0.33698352985603464
1104	52-53	0.30651314606950564
1104	54-55	0.33384578319515157
1104	56-57	0.27357581670786857
1104	58-59	0.23881205872690714
1104	60-61	0.2542184436087851
1104	62-63	0.27018860604582784
1104	64-65	0.2580391850074264
1104	66-67	0.30139617334338453
1104	68-69	0.31596835203851725
1104	70-71	0.25735380961450716
1104	72-73	0.20264145281366552
1104	74-75	0.27082643220902014
1104	76-77	0.21119813754606298
1104	78-79	0.21498079527888336
1104	80-81	0.2016190689315991
1104	82-83	0.20096877783537082
1104	84-85	0.20969131424297416
1104	86-87	0.19074494934453412
1104	88-89	0.154418496477021
1104	90-91	0.18756710292292667
1104	92-93	0.16830346997861056
1104	94-95	0.16870146798245855
1104	96-97	0.16957901070197323
1104	98-99	0.1698150889287433
1104	100	0.41028593904485433
1105	1	0.2235557317076271
1105	2	0.1872877225268681
1105	3	0.1603220325196446
1105	4	0.18095358498383973
1105	5	0.1630525051571894
1105	6	0.12289557399794404
1105	7	0.18519755852570796
1105	8	0.16863282046203665
1105	9	0.17962817965776878
1105	10-11	0.20570819140176155
1105	12-13	0.1950920008600434
1105	14-15	0.2880686944631847
1105	16-17	0.3013930102756248
1105	18-19	0.2331333494027774
1105	20-21	0.29166864095096656
1105	22-23	0.29551540407291554
1105	24-25	0.2766203999902501
1105	26-27	0.31558553412845214
1105	28-29	0.27749824900935494
1105	30-31	0.3000818067507538
1105	32-33	0.3188301797929043
1105	34-35	0.35596081687339165
1105	36-37	0.35720903961729533
1105	38-39	0.32082956999339274
1105	40-41	0.35657583931696735
1105	42-43	0.35989418895864844
1105	44-45	0.4314664845941252
1105	46-47	0.4218811538130609
1105	48-49	0.3894177998284789
1105	50-51	0.4013798411001659
1105	52-53	0.39964042833484825
1105	54-55	0.37733488822939165
1105	56-57	0.33614072306426834
1105	58-59	0.3689547754013489
1105	60-61	0.37400077117440134
1105	62-63	0.3696661470994158
1105	64-65	0.31871584715182877
1105	66-67	0.3428625505433587
1105	68-69	0.3112886452892738
1105	70-71	0.3299730201285982
1105	72-73	0.2837890973627992
1105	74-75	0.3293064510056425
1105	76-77	0.34140711477196817
1105	78-79	0.29807777738018615
1105	80-81	0.23844147878781285
1105	82-83	0.24929060940633718
1105	84-85	0.27278327615177034
1105	86-87	0.32721673980809385
1105	88-89	0.27710709963267277
1105	90-91	0.3053245075820996
1105	92-93	0.26172442681273367
1105	94-95	0.31050854874766287
1105	96-97	0.26754856551568906
1105	98-99	0.23655861299498326
1105	100	0.3956072133283932
1106	1	0.17270450126803638
1106	2	0.18226781398824698
1106	3	0.14751783431990617
1106	4	0.11769303146240873
1106	5	0.11318560782564902
1106	6	0.1359568564624567
1106	7	0.13230116555000393
1106	8	0.0910467528184924
1106	9	0.13334052564394483
1106	10-11	0.16101439252395977
1106	12-13	0.11911909810320509
1106	14-15	0.17480244317277283
1106	16-17	0.12374738876159341
1106	18-19	0.15633190284250986
1106	20-21	0.15374418617292207
1106	22-23	0.1821886241409416
1106	24-25	0.1435126604008019
1106	26-27	0.17944891392897233
1106	28-29	0.12745100742580462
1106	30-31	0.18311262065675038
1106	32-33	0.1839103639967945
1106	34-35	0.2384054800482147
1106	36-37	0.1849640443704601
1106	38-39	0.22409251170394384
1106	40-41	0.15407820893901913
1106	42-43	0.15358896353588136
1106	44-45	0.16489128007787457
1106	46-47	0.15946224548238064
1106	48-49	0.21312851423355283
1106	50-51	0.18535289889202033
1106	52-53	0.13261630692850446
1106	54-55	0.17697207611698218
1106	56-57	0.17637272786200242
1106	58-59	0.0916104625870986
1106	60-61	0.1530576291066339
1106	62-63	0.15783640664845677
1106	64-65	0.12192987624271012
1106	66-67	0.14387654454248633
1106	68-69	0.12073572032622337
1106	70-71	0.12140815932590243
1106	72-73	0.06729214143359741
1106	74-75	0.09861836010870917
1106	76-77	0.14959465794039772
1106	78-79	0.13951358963370808
1106	80-81	0.12449003306306139
1106	82-83	0.10293088035555797
1106	84-85	0.1225980000223359
1106	86-87	0.05538711308042821
1106	88-89	0.06521049452821615
1106	90-91	0.07488151358668915
1106	92-93	0.1371487307333581
1106	94-95	0.10873107691389805
1106	96-97	0.030720182781585947
1106	98-99	0.1036550531310354
1106	100	0.272984633545704
1107	1	0.09494118253306993
1107	2	0.12794023672104515
1107	3	0.1242111655892657
1107	4	0.07655737487470304
1107	5	0.023755468723983597
1107	6	0.028729865356986295
1107	7	0.10033026143623403
1107	8	0.05936821204031162
1107	9	0.024265252465667686
1107	10-11	0.0982270812926842
1107	12-13	-0.005733367511901122
1107	14-15	0.14061660595616132
1107	16-17	0.06980582990451722
1107	18-19	0.08849524566760891
1107	20-21	0.11049541491055948
1107	22-23	0.08433822688910197
1107	24-25	0.0709754936161957
1107	26-27	0.1809845183841432
1107	28-29	0.1047678295333867
1107	30-31	0.0960601229998943
1107	32-33	0.1739044743841518
1107	34-35	0.14865647023713535
1107	36-37	0.08971286950625057
1107	38-39	0.13346851741970056
1107	40-41	0.10557064995585108
1107	42-43	0.10759164756186834
1107	44-45	0.09497233841550212
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2216	74-75	-0.7790209736912814
2216	76-77	-0.8108412856237024
2216	78-79	-0.7661684029470592
2216	80-81	-0.8193855391648057
2216	82-83	-0.8266546710002487
2216	84-85	-0.8651222309735616
2216	86-87	-0.8173001176475907
2216	88-89	-0.7438005049631329
2216	90-91	-0.8031980579778732
2216	92-93	-0.8002014057562405
2216	94-95	-0.8209339854152873
2216	96-97	-0.8698969901934781
2216	98-99	-0.8409118813858427
2216	100	-0.28564646025262874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	13.0
13	61.0
14	286.0
15	860.0
16	1985.0
17	4186.0
18	7455.0
19	11767.0
20	16915.0
21	23695.0
22	32757.0
23	38320.0
24	39900.0
25	41833.0
26	48595.0
27	59685.0
28	75866.0
29	96255.0
30	121020.0
31	151234.0
32	191377.0
33	245728.0
34	351213.0
35	514586.0
36	865013.0
37	1617471.0
38	1240484.0
39	414054.0
40	615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12664582190946	21.596341228471555	12.556377653952458	25.720635295666522
2	14.688902513242565	20.677718059899906	38.77254624415575	25.860833182701775
3	17.579881520618976	26.905702715478398	29.06794256709832	26.446473196804305
4	11.92521056295851	16.909986368941933	38.899966204254525	32.26483686384503
5	12.696287845597142	38.830653819076375	35.05786791507147	13.41519042025501
6	30.06781176100221	38.687983333625716	17.40801763463088	13.836187270741188
7	26.30284934014976	32.00392774898974	23.946393082629513	17.746829828230997
8	26.950429832584742	34.00946013130362	19.48319015237317	19.556919883738466
9	26.478324569511557	13.993954516111017	21.38612860647589	38.14159230790154
10-11	26.193480914042656	25.475947868042294	26.944571368883157	21.385999849031897
12-13	26.218000860746145	23.245433039969328	27.540480659446143	22.99608543983838
14-15	23.5095054659568	24.155173423970115	25.199110329335245	27.13621078073784
16-17	22.19897435039053	26.678043459821964	26.563682707537282	24.559299482250225
18-19	21.53579600940091	27.166726149976	28.849965657945088	22.447512182678004
20-21	23.21775153128814	26.407701111482538	27.921100013471246	22.453447343758075
22-23	23.70734366472334	25.897057135954825	27.69697354018431	22.698625659137523
24-25	23.242312013729407	25.865881739828524	28.56398593131788	22.327820315124185
26-27	23.158160976741094	26.09633342255151	27.33197032849082	23.413535272216574
28-29	22.20192772426207	26.77798337837966	27.97183044640367	23.048258450954602
30-31	23.123428656224547	26.359449259346945	27.95847186158961	22.5586502228389
32-33	23.654021988230603	26.41677046394365	27.073996742114776	22.85521080571097
34-35	23.41202237224968	26.387502287456467	28.012461467323053	22.188013872970803
36-37	23.177595303443475	25.668778235099182	27.504231693870164	23.649394767587182
38-39	23.281076051710922	25.81194041813658	28.339682416545457	22.567301113607037
40-41	22.71962921718854	25.03208072190436	28.886813032764103	23.361477028142996
42-43	22.973989547792588	25.244144232157396	27.93611635037669	23.845749869673323
44-45	23.261617582987796	26.14612231667475	27.702936619309142	22.889323481028313
46-47	23.224149166786436	26.078428090496526	26.978152919744037	23.719269822973
48-49	24.260807537718286	25.928932650682825	26.893873125311735	22.916386686287154
50-51	23.313925294609103	25.691584397366075	27.01397967853451	23.980510629490315
52-53	22.822433988875034	26.342485466101138	26.676675411979563	24.158405133044262
54-55	22.835583342842607	26.30883953651827	26.515921743156905	24.339655377482224
56-57	24.188518280257636	25.65480000508592	26.17135877569697	23.985322938959474
58-59	22.717518251170908	26.25339165295869	27.348144037446538	23.680946058423867
60-61	22.866388561317432	26.92785704318509	26.439890472480272	23.76586392301721
62-63	23.49195660294729	26.038376478075104	25.88297429051832	24.58669262845929
64-65	22.482426004451632	26.396115964510237	26.46954799747024	24.651910033567894
66-67	23.328981056126672	25.853701521231535	26.434857274143976	24.38246014849782
68-69	22.66549475933061	25.70441990506392	27.00732452814823	24.622760807457244
70-71	22.630851676714215	25.530569270289256	26.84795625319833	24.990622799798203
72-73	23.246754678557238	25.409291021465783	27.431191604795167	23.912762695181808
74-75	23.301299175152817	25.906409269988632	26.853560352806273	23.93873120205227
76-77	23.055501660690574	25.860685793223283	26.259850253374715	24.82396229271143
78-79	22.8942719174613	25.917698859096017	26.36822130064052	24.819807922802163
80-81	22.408921410157966	26.495586114346587	27.090308700800374	24.005183774695073
82-83	23.382738101081998	25.163694971683825	28.281958844936284	23.171608082297894
84-85	23.568865033711113	25.78810419631776	26.624343558337504	24.018687211633623
86-87	21.947036269201558	26.471830365930266	27.229189698825074	24.351943666043105
88-89	23.068819405291897	25.180336871319646	26.95303717082556	24.797806552562893
90-91	22.594299940143884	25.219913690666356	27.03306796960616	25.152718399583595
92-93	23.08097893640879	25.433224538658056	26.841943638682146	24.643852886251004
94-95	21.795601420387744	25.4701054990212	27.625070555055636	25.10922252553542
96-97	22.213073290507534	25.308498812132108	27.08057946643881	25.39784843092155
98-99	21.47256508809504	25.98795989141241	26.97268877571467	25.566786244777877
100	22.355525493606468	25.163558475859183	25.997374947660003	26.48354108287435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	242.0
1	167.0
2	130.0
3	156.5
4	108.5
5	94.5
6	203.0
7	407.0
8	614.0
9	703.5
10	544.0
11	375.0
12	408.5
13	509.0
14	604.0
15	811.0
16	1087.0
17	1423.0
18	1977.0
19	2752.0
20	3623.5
21	4566.5
22	5664.0
23	6822.0
24	7961.5
25	9210.5
26	11480.5
27	14006.0
28	17793.5
29	26959.5
30	41822.0
31	55171.0
32	74994.0
33	95564.0
34	108139.0
35	123588.0
36	136233.0
37	160951.5
38	174969.5
39	167200.5
40	156097.0
41	144216.5
42	143825.5
43	154395.5
44	175418.5
45	218680.0
46	258901.0
47	289428.0
48	314940.5
49	309832.5
50	291789.0
51	300189.0
52	310060.5
53	300218.0
54	289460.0
55	249450.5
56	214889.5
57	178416.5
58	138601.5
59	137344.5
60	115372.0
61	76537.5
62	55861.0
63	46863.5
64	34420.0
65	16280.0
66	8822.0
67	6057.5
68	4319.5
69	3235.5
70	2437.0
71	1863.5
72	1408.0
73	1219.0
74	954.0
75	676.0
76	436.5
77	213.5
78	100.5
79	55.5
80	23.0
81	5.5
82	2.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008079529610429868
3	0.0
4	0.08612263534942276
5	0.004683552025169505
6	6.437872199545711E-5
7	8.047340249432139E-5
8	0.0
9	0.0
10-11	0.0
12-13	3.057989294784213E-4
14-15	0.009608524257821974
16-17	0.0
18-19	8.852074274375353E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.4142020748296416E-5
60-61	0.0
62-63	0.0
64-65	7.242606224488926E-5
66-67	1.6899414523807493E-4
68-69	0.0
70-71	3.9431967222217486E-4
72-73	3.1384626972785345E-4
74-75	3.46035630725582E-4
76-77	7.242606224488926E-5
78-79	3.2189360997728556E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06169091035214678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6213233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.52831507544837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43972365307688	42.6359778710095
2	16.572670913285243	20.06231693362096
3	6.324127693832393	11.483663808889673
4	2.8020962163158676	6.784246506115542
5	1.4276542013672604	4.320675165957258
6	0.8042757809504478	2.92088747261526
7	0.48583553115336175	2.0584764273148948
8	0.317342516328736	1.536656625214508
9	0.2028938485704305	1.1052740513827195
>10	0.6101763842796579	5.308251307763215
>50	0.0077380237743760695	0.32324946435323765
>100	0.004680122292655303	0.5800229656524374
>500	4.1321836018083023E-4	0.1799820995038294
>1k	2.8925276531089516E-4	0.30653976243717485
>5k	8.264364723163316E-5	0.3937795381698056
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8463	0.1362092810618884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7281	0.11718536871223081	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	8.047340249432139E-6	0.0
22-23	0.0	0.0	0.0	6.437872199545713E-5	0.0
24-25	0.0	0.0	0.0	1.207101037414821E-4	0.0
26-27	0.0	0.0	0.0	5.150297759636569E-4	0.0
28-29	0.0	0.0	0.0	0.0026556222823126063	0.0
30-31	0.0	0.0	0.0	0.010630536469499855	0.0
32-33	0.0	0.0	0.0	0.026862021752604482	0.0
34-35	0.0	0.0	0.0	0.052170906837068556	0.0
36-37	0.0	0.0	0.0	0.09762228456586128	0.0
38-39	0.0	0.0	0.0	0.16534870010508218	0.0
40-41	0.0	0.0	0.0	0.23989121283557208	0.0
42-43	0.0	0.0	0.0	0.3175882829438394	0.0
44-45	0.0	0.0	0.0	0.41239399842239943	0.0
46-47	0.0	0.0	0.0	0.5331926229066253	0.0
48-49	0.0	0.0	0.0	0.6703756321386949	0.0
50-51	0.0	0.0	0.0	0.8170786448858429	0.0
52-53	0.0	0.0	0.0	0.9650853267533988	0.0
54-55	0.0	0.0	0.0	1.1248411253851254	0.0
56-57	0.0	0.0	0.0	1.322258476384195	0.0
58-59	0.0	0.0	0.0	1.5628015237799708	0.0
60-61	0.0	0.0	0.0	1.8146188948652013	0.0
62-63	0.0	0.0	0.0	2.0808812416981626	0.0
64-65	0.0	0.0	0.0	2.3590616994405327	0.0
66-67	0.0	0.0	0.0	2.6769557813138505	0.0
68-69	0.0	0.0	0.0	3.033613901168683	0.0
70-71	0.0	0.0	0.0	3.4262355845982277	0.0
72-73	0.0	0.0	0.0	3.8394745537468173	0.0
74-75	0.0	0.0	0.0	4.248939320318424	0.0
76-77	0.0	0.0	0.0	4.717680795167347	0.0
78-79	0.0	0.0	0.0	5.223319325059917	0.0
80-81	0.0	0.0	0.0	5.772164024751687	0.0
82-83	0.0	0.0	0.0	6.345979621237446	0.0
84-85	0.0	0.0	0.0	6.90229225268069	0.0
86-87	0.0	0.0	0.0	7.499968213006015	0.0
88	0.0	0.0	0.0	7.979179277519449	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	7400	0.0	41.24149	94
GTACTAG	7780	0.0	33.441616	1
GTATCAA	21065	0.0	31.826958	1
CAGTACG	1085	0.0	31.192436	9
CTAACGC	3865	0.0	30.793837	3
TAGGCAT	9610	0.0	30.517704	5
CTGTGCG	10515	0.0	30.442787	9
TAACGCC	3960	0.0	30.055098	4
TCTAACG	3970	0.0	29.982773	2
TAGTACT	10795	0.0	29.807545	4
ACTGTGC	11210	0.0	29.347363	8
CAACGCA	21845	0.0	28.743856	5
CGCCTAC	4115	0.0	28.552216	7
GTACTGT	11555	0.0	28.22664	6
TACCCCG	500	0.0	28.199533	5
ATCAACG	22495	0.0	27.876415	3
CTAGGCA	10970	0.0	27.616714	4
GGTATCA	14000	0.0	27.455906	1
TACTGTG	12975	0.0	27.093426	7
TATTCTC	2905	0.0	26.69491	5
>>END_MODULE