FastQCFastQC Report
Mon 16 Jan 2017
ERR1146683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324254
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC65450.040.30320794
GTACTAG68950.034.8492471
TAGTACT83900.033.4409034
CTGTGCG84750.033.3219269
ACTGTGC92000.031.4114258
TAGGCAT80500.031.2888345
GCATCGT72050.030.5219468
GTACTGT93450.030.2703576
TACTGTG101900.029.1436047
AGGCATC77100.029.0712956
CTAGTAC103900.028.5426653
TACCCCG4000.028.1949735
CTAGGCA92950.027.9602724
TCTAACG31250.027.8229032
AAGCTAA27400.027.7817619
CGTTAAC9150.027.7510531
CTAACGC32400.026.5452483
GTCTAAC33600.026.3102741
TAACGCC33200.025.6215934
CGCCTAC33000.025.4882167
Produced by FastQC (version 0.11.5)