##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146683_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4324254
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643862270810178	31.0	31.0	34.0	30.0	34.0
2	31.730628219341416	33.0	31.0	34.0	30.0	34.0
3	31.833077335420167	34.0	31.0	34.0	30.0	34.0
4	35.26937062438978	37.0	35.0	37.0	32.0	37.0
5	35.43883453654665	37.0	35.0	37.0	33.0	37.0
6	35.501206219616144	37.0	35.0	37.0	33.0	37.0
7	35.4880476493749	37.0	35.0	37.0	33.0	37.0
8	35.47334546028055	37.0	35.0	37.0	33.0	37.0
9	37.064310745853504	39.0	37.0	39.0	33.0	39.0
10-11	36.91148172609657	39.0	37.0	39.0	32.5	39.0
12-13	36.86599249257792	39.0	37.0	39.0	32.5	39.0
14-15	38.088454216611694	40.0	38.0	41.0	32.5	41.0
16-17	38.091491966013095	40.0	38.0	41.0	33.0	41.0
18-19	38.01975334936384	40.0	38.0	41.0	32.0	41.0
20-21	38.065320977907405	40.0	38.0	41.0	32.5	41.0
22-23	37.95673519640613	40.0	38.0	41.0	32.0	41.0
24-25	37.87953829724156	40.0	37.5	41.0	32.0	41.0
26-27	37.89885538638572	40.0	38.0	41.0	32.0	41.0
28-29	37.846616086844115	40.0	37.0	41.0	32.0	41.0
30-31	37.68332641884589	40.0	37.0	41.0	32.0	41.0
32-33	37.67794710948987	40.0	37.0	41.0	32.0	41.0
34-35	37.59806812920795	40.0	37.0	41.0	31.0	41.0
36-37	37.46540177797141	40.0	37.0	41.0	31.0	41.0
38-39	37.34964608924453	40.0	36.0	41.0	31.0	41.0
40-41	37.24618997866453	40.0	36.0	41.0	31.0	41.0
42-43	37.04979147848392	39.5	36.0	41.0	30.0	41.0
44-45	36.86130324444402	39.0	35.0	41.0	30.0	41.0
46-47	36.83162217113056	39.0	35.0	41.0	30.0	41.0
48-49	36.63209284190984	39.0	35.0	41.0	30.0	41.0
50-51	36.39955354148947	39.0	35.0	40.5	29.5	41.0
52-53	36.1365164950995	38.0	35.0	40.0	29.0	41.0
54-55	35.84395655296844	38.0	34.5	40.0	29.0	41.0
56-57	35.54169910000661	37.0	34.0	40.0	28.5	41.0
58-59	35.163135421739796	36.5	34.0	40.0	28.0	41.0
60-61	34.85871088978584	36.0	34.0	39.0	28.0	41.0
62-63	34.48851640074797	35.0	33.0	39.0	27.0	41.0
64-65	34.29243841827978	35.0	33.0	38.5	27.0	40.0
66-67	34.00299092051485	35.0	33.0	37.5	27.0	40.0
68-69	33.84030447795158	35.0	33.0	37.0	27.0	40.0
70-71	33.751875583626685	35.0	33.0	36.5	27.0	39.0
72-73	33.52182931437423	35.0	33.0	36.0	27.0	39.0
74-75	33.290333546549306	35.0	33.0	36.0	27.0	38.5
76-77	33.06435248715732	35.0	33.0	35.0	26.5	37.0
78-79	32.847508148226254	35.0	33.0	35.0	26.0	37.0
80-81	32.722003957214355	35.0	33.0	35.0	26.0	36.5
82-83	32.52757677971738	35.0	33.0	35.0	26.0	36.0
84-85	32.429368857611045	35.0	33.0	35.0	26.0	36.0
86-87	32.327599974469585	35.0	33.0	35.0	25.0	36.0
88-89	32.261614835761264	35.0	33.0	35.0	25.0	35.0
90-91	32.16025793119461	35.0	32.5	35.0	25.0	35.0
92-93	32.03907425419506	35.0	32.0	35.0	25.0	35.0
94-95	32.01642456710452	35.0	32.0	35.0	25.0	35.0
96-97	31.933476618163503	35.0	32.0	35.0	25.0	35.0
98-99	31.864618613985208	35.0	32.0	35.0	25.0	35.0
100	30.882344792882193	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3448206567965002
1101	2	0.44984238839289503
1101	3	0.4965977259838965
1101	4	0.5254440435146819
1101	5	0.3744096844390228
1101	6	0.38026361048653
1101	7	0.4118827443166424
1101	8	0.4332686365744536
1101	9	0.6088816964554127
1101	10-11	0.6207255583564333
1101	12-13	0.4643838292474456
1101	14-15	0.6773387918434395
1101	16-17	0.6803776155282009
1101	18-19	0.6748796914957182
1101	20-21	0.6449466640095807
1101	22-23	0.6641785362091426
1101	24-25	0.7153231516637888
1101	26-27	0.7033294518645121
1101	28-29	0.6868107972624742
1101	30-31	0.6935186860253495
1101	32-33	0.6366395745400197
1101	34-35	0.6722412172256895
1101	36-37	0.6909895115163707
1101	38-39	0.6529848900905719
1101	40-41	0.6692276489007938
1101	42-43	0.6560354795374863
1101	44-45	0.7137646294771969
1101	46-47	0.6783994700440843
1101	48-49	0.5950469514592314
1101	50-51	0.5431594043871897
1101	52-53	0.5763944404510895
1101	54-55	0.5407611265162586
1101	56-57	0.5283705600303392
1101	58-59	0.5375406851189695
1101	60-61	0.5630638714403702
1101	62-63	0.5769605780838845
1101	64-65	0.5593749459212063
1101	66-67	0.5566343899860513
1101	68-69	0.5362631099499708
1101	70-71	0.5567028439707755
1101	72-73	0.5080691386366212
1101	74-75	0.530919447424516
1101	76-77	0.551137593405997
1101	78-79	0.5393504011576624
1101	80-81	0.4756672904108683
1101	82-83	0.49819191255203066
1101	84-85	0.497533307592704
1101	86-87	0.48788743028916315
1101	88-89	0.5008128655450506
1101	90-91	0.4902517028925715
1101	92-93	0.5030281988961818
1101	94-95	0.526896693205039
1101	96-97	0.45793520669588617
1101	98-99	0.4576813311758734
1101	100	0.6019848655657043
1102	1	0.2469739231266992
1102	2	0.26717969500148797
1102	3	0.2946736514020927
1102	4	0.4050425000352007
1102	5	0.2516270740296349
1102	6	0.2664053928842094
1102	7	0.30156865192233795
1102	8	0.30151079846590534
1102	9	0.4519103315124724
1102	10-11	0.5065465940318674
1102	12-13	0.4071427843324713
1102	14-15	0.6023904237478064
1102	16-17	0.5700459598815613
1102	18-19	0.5656732432896305
1102	20-21	0.4942933458011254
1102	22-23	0.5590692817663054
1102	24-25	0.6128960608977181
1102	26-27	0.6240256870103735
1102	28-29	0.6176348847703608
1102	30-31	0.6058345834350902
1102	32-33	0.5405443550486311
1102	34-35	0.5646392271874774
1102	36-37	0.6219832032366597
1102	38-39	0.6331833954172197
1102	40-41	0.5412699363434186
1102	42-43	0.5828854029953234
1102	44-45	0.6004720877152039
1102	46-47	0.5493609218932392
1102	48-49	0.5144572145428441
1102	50-51	0.4974136240600018
1102	52-53	0.4156182838123783
1102	54-55	0.4359871389102352
1102	56-57	0.48281096177090177
1102	58-59	0.43530881888718653
1102	60-61	0.37843603559824146
1102	62-63	0.36611208171274967
1102	64-65	0.3702578152581495
1102	66-67	0.44650842147122205
1102	68-69	0.36437318227231685
1102	70-71	0.34620422149781405
1102	72-73	0.3142895731039559
1102	74-75	0.36106143733520923
1102	76-77	0.38317795062980053
1102	78-79	0.34201181353297017
1102	80-81	0.38687421044822656
1102	82-83	0.36804464786918345
1102	84-85	0.4006040944553959
1102	86-87	0.35801758384300086
1102	88-89	0.30398691621648055
1102	90-91	0.30132252221539346
1102	92-93	0.3640903525218917
1102	94-95	0.36702466729257566
1102	96-97	0.4567466730071956
1102	98-99	0.37936299230016957
1102	100	0.39474834188400365
1103	1	0.18581549491401006
1103	2	0.2594459336527919
1103	3	0.2835094482737617
1103	4	0.39650254911204996
1103	5	0.18609602726019858
1103	6	0.30246283101811855
1103	7	0.21321136427677345
1103	8	0.23948065596028556
1103	9	0.35505120454917716
1103	10-11	0.47926404798365496
1103	12-13	0.43722874686050517
1103	14-15	0.49572258128750946
1103	16-17	0.4767040545112806
1103	18-19	0.5386111798313991
1103	20-21	0.5035157725654074
1103	22-23	0.5356855274127668
1103	24-25	0.5326437325567284
1103	26-27	0.47048038542214954
1103	28-29	0.47361226299271664
1103	30-31	0.46895875320228697
1103	32-33	0.4456478622528053
1103	34-35	0.43966068802262726
1103	36-37	0.4000306914888867
1103	38-39	0.4757299232172727
1103	40-41	0.4137141105035198
1103	42-43	0.44946711549760465
1103	44-45	0.36829559153672164
1103	46-47	0.3612795106230422
1103	48-49	0.3366689448012181
1103	50-51	0.3218351321728363
1103	52-53	0.3351250265584724
1103	54-55	0.3263285286529083
1103	56-57	0.32170010921457504
1103	58-59	0.30751850593216545
1103	60-61	0.31094121954278364
1103	62-63	0.31598408290689406
1103	64-65	0.23787136471426606
1103	66-67	0.2446545032831935
1103	68-69	0.2790253209349913
1103	70-71	0.25338001532168875
1103	72-73	0.16939032764563677
1103	74-75	0.19310308142956956
1103	76-77	0.20396604357671322
1103	78-79	0.22041738912179198
1103	80-81	0.198921762834658
1103	82-83	0.2940383945010723
1103	84-85	0.3183686004901105
1103	86-87	0.294155785661971
1103	88-89	0.3539953870386867
1103	90-91	0.27935271409867113
1103	92-93	0.29934632601233346
1103	94-95	0.2386779476007419
1103	96-97	0.3247455668341601
1103	98-99	0.30028142523495305
1103	100	0.3968756959425228
1104	1	0.2583692719770774
1104	2	0.28918561659432385
1104	3	0.23023789605247202
1104	4	0.3430901184114248
1104	5	0.2642925773058522
1104	6	0.2477759705665079
1104	7	0.2614366077293582
1104	8	0.3224621886653978
1104	9	0.33551678640991867
1104	10-11	0.44304396887240927
1104	12-13	0.3277588122017505
1104	14-15	0.4563793026275178
1104	16-17	0.4999067261779615
1104	18-19	0.44567652010289294
1104	20-21	0.39191811484015204
1104	22-23	0.4788487086903359
1104	24-25	0.4802541906532909
1104	26-27	0.38182199130633876
1104	28-29	0.4126881084960772
1104	30-31	0.41049333761979057
1104	32-33	0.47397044648876374
1104	34-35	0.4501296939937873
1104	36-37	0.32792884981159176
1104	38-39	0.3794232989168549
1104	40-41	0.4431225277636486
1104	42-43	0.4195010137744575
1104	44-45	0.28445515813622535
1104	46-47	0.3023819659197997
1104	48-49	0.30093187761911366
1104	50-51	0.20647227154301362
1104	52-53	0.3112115362157297
1104	54-55	0.31003953428790965
1104	56-57	0.2967366508713667
1104	58-59	0.34252636389778957
1104	60-61	0.30037048959377444
1104	62-63	0.32016707725052385
1104	64-65	0.2557649718987136
1104	66-67	0.28591460368027555
1104	68-69	0.26807600956703936
1104	70-71	0.31367747571992055
1104	72-73	0.26920776856793793
1104	74-75	0.2297331377351597
1104	76-77	0.19042694160970086
1104	78-79	0.29762103042102694
1104	80-81	0.3074264092567347
1104	82-83	0.2899567355049939
1104	84-85	0.2882381220348691
1104	86-87	0.27063790707256885
1104	88-89	0.32208996466071227
1104	90-91	0.3407244180107014
1104	92-93	0.31127300091518606
1104	94-95	0.38591733317070265
1104	96-97	0.3423464660136162
1104	98-99	0.36197338826344705
1104	100	0.38416693034474036
1105	1	0.07640437971891245
1105	2	0.15920278053443582
1105	3	0.15663535046464006
1105	4	0.26113796936152767
1105	5	0.133552702085332
1105	6	0.0912886420180925
1105	7	0.10341790128438788
1105	8	0.15984455662963626
1105	9	0.26764373020603216
1105	10-11	0.2356113809720881
1105	12-13	0.1954576706165838
1105	14-15	0.2772781398280415
1105	16-17	0.24470444744216735
1105	18-19	0.32229724868140863
1105	20-21	0.2239865081018877
1105	22-23	0.2413499462597244
1105	24-25	0.24006278587823005
1105	26-27	0.289756453692803
1105	28-29	0.2929065395935311
1105	30-31	0.33578763953766355
1105	32-33	0.2823852035625265
1105	34-35	0.2900180638142018
1105	36-37	0.31046882705899037
1105	38-39	0.30393428743651896
1105	40-41	0.2738515440488811
1105	42-43	0.32629346053623465
1105	44-45	0.3153874484919683
1105	46-47	0.2838302018951069
1105	48-49	0.18909296606615555
1105	50-51	0.2630521568801285
1105	52-53	0.2089448126635105
1105	54-55	0.2834173965655822
1105	56-57	0.3240958885724865
1105	58-59	0.34038072925068263
1105	60-61	0.32976612283985673
1105	62-63	0.3096698898934349
1105	64-65	0.32984942842524134
1105	66-67	0.23413433525097105
1105	68-69	0.24861849500920385
1105	70-71	0.20672277031260222
1105	72-73	0.21189719952642605
1105	74-75	0.17411264785828706
1105	76-77	0.18537181078714582
1105	78-79	0.22803243300155884
1105	80-81	0.1316493639088634
1105	82-83	0.1611157526324689
1105	84-85	0.07288050123490564
1105	86-87	0.08451254817745024
1105	88-89	0.12191479224770063
1105	90-91	0.08935237082688019
1105	92-93	0.12374897541934615
1105	94-95	0.21451683671350708
1105	96-97	0.0666533276667991
1105	98-99	0.11347095889139425
1105	100	0.14969612151000078
1106	1	0.029837152513984222
1106	2	0.11040652457652556
1106	3	0.08605406536699789
1106	4	0.23414039616999105
1106	5	0.02796863840046626
1106	6	0.08887254373928499
1106	7	0.05073122745402259
1106	8	0.08289786174844238
1106	9	0.2365591714411579
1106	10-11	0.23158016007493387
1106	12-13	0.11280363612697641
1106	14-15	0.20439556316904373
1106	16-17	0.15315516177047783
1106	18-19	0.1712636753286958
1106	20-21	0.23369486737897205
1106	22-23	0.20884434672687036
1106	24-25	0.20935590324582876
1106	26-27	0.2359563414510859
1106	28-29	0.23608247060452925
1106	30-31	0.286781978501665
1106	32-33	0.21613394848628786
1106	34-35	0.19853474593642773
1106	36-37	0.1643685291502166
1106	38-39	0.2106482950853632
1106	40-41	0.25030085611016517
1106	42-43	0.17851651211733355
1106	44-45	0.1489929391693039
1106	46-47	0.11740223871369437
1106	48-49	0.037346312274465276
1106	50-51	0.13201367663167218
1106	52-53	0.04591189052942468
1106	54-55	0.10917035759074167
1106	56-57	0.09015560206547946
1106	58-59	0.0911998540932899
1106	60-61	0.13106808518508473
1106	62-63	0.1311400025870597
1106	64-65	0.06991814030129717
1106	66-67	0.06347540504576443
1106	68-69	0.09826175320084474
1106	70-71	0.1046861884376753
1106	72-73	0.11041149943709172
1106	74-75	0.14000706342358882
1106	76-77	0.10610920605957119
1106	78-79	0.09303091341722336
1106	80-81	0.1312570942261999
1106	82-83	0.17012678755782673
1106	84-85	0.1623654921271651
1106	86-87	0.18340600088998826
1106	88-89	0.1894408186696026
1106	90-91	0.1841340412981296
1106	92-93	0.15974099883214876
1106	94-95	0.13540870100467117
1106	96-97	0.22536822853986394
1106	98-99	0.15574611167287955
1106	100	0.15260911780603692
1107	1	0.10377190355396948
1107	2	0.12888225317309931
1107	3	0.15291522591058282
1107	4	0.20043344614283143
1107	5	0.16446415093133027
1107	6	0.18483181090615375
1107	7	0.17139678483459164
1107	8	0.15397016926101514
1107	9	0.18461731402225467
1107	10-11	0.2482909887963558
1107	12-13	0.14167866012939356
1107	14-15	0.2610597623760782
1107	16-17	0.2055081831474368
1107	18-19	0.1438925763002885
1107	20-21	0.1832700931324922
1107	22-23	0.2719887326137638
1107	24-25	0.31644700628117306
1107	26-27	0.19627320181452035
1107	28-29	0.24932396066461138
1107	30-31	0.37161491815977854
1107	32-33	0.29507454933293786
1107	34-35	0.23585744040234147
1107	36-37	0.21943392396874373
1107	38-39	0.23646578682817676
1107	40-41	0.2301348105913661
1107	42-43	0.3023657229704497
1107	44-45	0.31835282493805295
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2216	86-87	-1.0383584245351933
2216	88-89	-1.042265534355586
2216	90-91	-1.088270210725014
2216	92-93	-1.0154324887238104
2216	94-95	-1.0425784314580682
2216	96-97	-1.0800397953653231
2216	98-99	-1.0757737831437062
2216	100	-0.5833098661931153
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	2.0
2	9.0
3	16.0
4	43.0
5	24.0
6	39.0
7	165.0
8	498.0
9	867.0
10	1061.0
11	1607.0
12	2555.0
13	3989.0
14	5930.0
15	8352.0
16	10470.0
17	12158.0
18	13998.0
19	15999.0
20	17980.0
21	20802.0
22	24627.0
23	28536.0
24	33568.0
25	39099.0
26	45032.0
27	52220.0
28	61243.0
29	73905.0
30	89592.0
31	108296.0
32	136033.0
33	177889.0
34	256700.0
35	385127.0
36	666710.0
37	1159444.0
38	745571.0
39	124045.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34923998886553	18.88665443540559	11.191904691496173	24.57220088423271
2	17.02122738873169	19.284834622487367	37.862806566764455	25.83113142201649
3	20.2185901035456	24.610882973025227	27.55332464363125	27.617202279797926
4	13.216002279458461	15.32482514361097	38.175101079273624	33.28407149765694
5	14.132955543036548	38.16565419977188	33.62171757677681	14.079672680414756
6	32.11467153483859	36.33620265014424	16.887234508024946	14.661891306992231
7	28.596219198166605	30.674399862171516	22.020482330318913	18.708898609342967
8	27.945027252572107	33.191132929511014	18.747258150044203	20.116581667872676
9	26.641203879821994	14.449036924355587	20.17252132697257	38.737237868849846
10-11	27.652189557953566	24.277171722404155	25.694291398536016	22.37634732110626
12-13	27.34740878833895	21.946981915788005	26.014758888928846	24.6908504069442
14-15	24.728105872755897	23.261469192038614	23.434990712256873	28.575434222948616
16-17	24.060588891651104	25.988760991854033	24.486191167203373	25.46445894929149
18-19	23.19179941915019	27.033115978133665	25.981309988953978	23.793774613762164
20-21	25.29135093940291	26.243235902423322	25.043721864077124	23.421691294096643
22-23	25.770528918032333	25.481412190217622	24.9946637590031	23.75339513274694
24-25	25.318038486411886	25.2396078212359	25.73105115476233	23.711302537589887
26-27	25.199856617562038	25.672162993455284	24.279526375430727	24.848454013551954
28-29	23.925885137897794	26.194730337515193	25.408341951752885	24.471042572834126
30-31	24.55465966948152	26.00145998247697	25.54846763703582	23.895412711005683
32-33	24.9318308463729	25.871160791089427	24.50285170871833	24.69415665381934
34-35	25.012097877815425	26.051746424780802	25.28090491779167	23.655250779612107
36-37	24.50707251450749	25.153909567237715	24.523932346512794	25.815085571741996
38-39	24.752580119845728	25.645311820106286	25.07419387552034	24.527914184527642
40-41	24.485208072192748	24.51353845299932	26.05434113941323	24.946912335394707
42-43	24.272974603867	24.69223063777014	25.582562816127535	25.452231942235322
44-45	24.976491159045132	25.075152540024593	25.470896743017924	24.477459557912358
46-47	24.554636636231	25.472760554336876	24.710542725739433	25.262060083692695
48-49	25.533639094393056	25.38188039003907	24.80925207662045	24.275228438947423
50-51	24.463596842383183	24.863693940754032	25.693679157422988	24.97903005943979
52-53	24.679587117977263	25.083353149030387	25.130367239610173	25.106692493382177
54-55	24.11551068168839	24.928419052756066	25.34934671037582	25.606723555179723
56-57	25.50725596360332	24.725080352943504	24.564345554721243	25.203318128731933
58-59	24.0323294487773	25.360978755928226	25.600345646808126	25.00634614848634
60-61	24.11215082967074	25.605529249384624	25.183110416723288	25.099209504221353
62-63	24.644444261990056	25.136469778210373	24.51456886073335	25.704517099066216
64-65	23.8632270243273	25.43686767326659	25.21079147830357	25.489113824102542
66-67	24.697032529698873	25.174268309013982	24.907098147883882	25.221601013403262
68-69	24.179702076087235	24.844858035577282	25.65830181080191	25.317138077533574
70-71	23.84025963798836	24.818643221292795	25.2556016704031	26.085495470315745
72-73	24.560561792633596	24.54392161841941	25.975936388510952	24.919580200436044
74-75	24.815738141395247	24.721780089357868	25.48289234916639	24.979589420080494
76-77	24.568659571500827	24.734798575971215	24.696255992140227	26.000285860387727
78-79	24.839451835550665	24.823215750852423	24.419059821992843	25.91827259160407
80-81	24.23110351695756	25.341875676778308	25.389218357766353	25.037802448497775
82-83	25.032996442374117	24.508221762920073	26.581533879546736	23.877247915159074
84-85	24.698596084987024	24.68884763884077	25.660049595653415	24.952506680518788
86-87	23.518959442254385	25.313916927912373	26.095214086568987	25.071909543264255
88-89	24.976991159447827	23.938352612114834	25.63277925169781	25.451876976739534
90-91	24.33335384065992	24.486048619016582	25.120453560387983	26.060143979935507
92-93	24.78975387969225	24.388098969879003	25.774475636473976	25.04767151395477
94-95	23.888674256147226	24.561038140475887	26.431168359014677	25.11911924436221
96-97	24.085813952896736	24.40393550238805	25.991143477559998	25.51910706715522
98-99	23.656726057306376	24.580675533112487	25.78677073182465	25.97582767775649
100	24.369104390100045	23.937390468908415	25.124516811247517	26.568988329744027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	53.0
2	37.0
3	57.0
4	72.0
5	66.0
6	65.0
7	65.0
8	73.0
9	82.0
10	75.0
11	72.0
12	76.5
13	82.5
14	98.0
15	118.5
16	123.5
17	144.0
18	173.0
19	223.0
20	298.5
21	366.5
22	490.0
23	664.0
24	864.5
25	1184.0
26	1595.5
27	2053.5
28	2772.0
29	4054.0
30	6094.0
31	8314.0
32	11450.5
33	15111.5
34	18891.0
35	23834.5
36	30623.0
37	42376.0
38	54305.5
39	66260.5
40	75161.5
41	82570.0
42	93682.0
43	109605.5
44	134131.5
45	170130.0
46	205617.5
47	236866.5
48	263302.0
49	268396.5
50	255261.5
51	260242.5
52	269422.5
53	260550.0
54	244764.0
55	209735.5
56	183279.0
57	151046.5
58	117372.5
59	114040.5
60	95248.5
61	64922.5
62	48516.5
63	41229.5
64	30607.5
65	15094.5
66	8354.5
67	5679.5
68	4052.0
69	2991.0
70	2326.0
71	1755.0
72	1309.0
73	1067.0
74	803.0
75	596.0
76	406.0
77	188.5
78	96.5
79	70.0
80	54.5
81	40.0
82	37.5
83	32.0
84	29.0
85	25.5
86	19.5
87	18.5
88	11.0
89	11.5
90	11.5
91	10.0
92	8.0
93	7.0
94	8.5
95	6.0
96	6.0
97	6.0
98	5.5
99	5.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05876158061020467
2	0.008302010011437811
3	0.008371386139667097
4	0.009712657952099945
5	0.003746310924381408
6	0.002590042120559986
7	9.018896669807094E-4
8	0.0017344032057321332
9	8.093881626749954E-4
10-11	5.78134401910711E-4
12-13	2.890672009553555E-4
14-15	1.5031494449678487E-4
16-17	6.706359062164249E-4
18-19	0.0016765897655410622
20-21	0.0010290792354010656
22-23	0.0011331434277449938
24-25	0.0012140822440124932
26-27	0.0035613079157699803
28-29	0.004208818445909976
30-31	0.0053072738095403275
32-33	0.006186038100444609
34-35	0.0034341183473496238
36-37	0.008498575708087453
38-39	0.006440417237285322
40-41	0.006336353044941394
42-43	0.014869616817143489
44-45	0.020361893635295246
46-47	0.018916557630518464
48-49	0.021818792328110233
50-51	0.018581239677410256
52-53	0.025021656914695573
54-55	0.01912468601520632
56-57	0.01555181541139813
58-59	0.017702475386505972
60-61	0.01622245131761455
62-63	0.011435498469793866
64-65	0.01134299696548815
66-67	0.013990852526239207
68-69	0.005330399185616756
70-71	0.008961083229616022
72-73	0.008810768285119237
74-75	0.011932694055437077
76-77	0.010753299875539225
78-79	0.012753644906150286
80-81	0.010637672995157084
82-83	0.007006988951157819
84-85	0.011285183525297079
86-87	0.009689532576023518
88-89	0.008614202588469594
90-91	0.01378272414155135
92-93	0.009088272798036378
94-95	0.007642936793259601
96-97	0.010059538593246374
98-99	0.009816722144443873
100	0.25393975469526076
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4324254.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.29307346039431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34949778329485	47.15864649264406
2	15.352429912618803	19.741098083351115
3	5.444639415103703	10.501578056418621
4	2.475601715839483	6.366561719005843
5	1.2662442216085907	4.0705366379340475
6	0.7451684260453219	2.874550101365897
7	0.42251068164521177	1.9015157204971815
8	0.27046932668358487	1.3911443431400887
9	0.1770583770365174	1.0245264517428305
>10	0.4907977739534647	4.501426993751663
>50	0.004470095844259067	0.18673675448013105
>100	0.0010073541281722996	0.09722609758771493
>500	0.0	0.0
>1k	1.049161980203296E-4	0.18445254808081554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6250752152856883E-5
2	0.0	0.0	0.0	0.0	4.6250752152856883E-5
3	0.0	0.0	0.0	0.0	4.6250752152856883E-5
4	0.0	0.0	0.0	0.0	4.6250752152856883E-5
5	0.0	0.0	0.0	0.0	4.6250752152856883E-5
6	0.0	0.0	0.0	0.0	4.6250752152856883E-5
7	0.0	0.0	0.0	0.0	4.6250752152856883E-5
8	0.0	0.0	0.0	0.0	4.6250752152856883E-5
9	0.0	0.0	0.0	0.0	4.6250752152856883E-5
10-11	0.0	0.0	0.0	0.0	8.093881626749955E-5
12-13	0.0	0.0	0.0	0.0	1.8500300861142753E-4
14-15	0.0	0.0	0.0	2.3125376076428442E-5	2.0812838468785598E-4
16-17	0.0	0.0	0.0	2.3125376076428442E-5	2.890672009553555E-4
18-19	0.0	0.0	0.0	2.3125376076428442E-5	3.237552650699982E-4
20-21	0.0	0.0	0.0	1.3875225645857065E-4	4.0469408133749776E-4
22-23	0.0	0.0	0.0	1.734403205732133E-4	4.1625676937571195E-4
24-25	0.0	0.0	0.0	2.5437913684071286E-4	4.1625676937571195E-4
26-27	0.0	0.0	0.0	7.284493464074959E-4	4.856328976049973E-4
28-29	0.0	0.0	0.0	0.003538182539693552	4.856328976049973E-4
30-31	0.0	0.0	0.0	0.012638018025768144	4.856328976049973E-4
32-33	0.0	0.0	0.0	0.0307336248055734	4.856328976049973E-4
34-35	0.0	0.0	0.0	0.06100474208961823	5.781344019107111E-4
36-37	0.0	0.0	0.0	0.11573094457448614	5.781344019107111E-4
38-39	0.0	0.0	0.0	0.19947949403527176	5.781344019107111E-4
40-41	0.0	0.0	0.0	0.2918769341486416	5.781344019107111E-4
42-43	0.0	0.0	0.0	0.3859625267155907	5.781344019107111E-4
44-45	0.0	0.0	0.0	0.5043528895388661	6.012597779871396E-4
46-47	0.0	0.0	0.0	0.6545868952193835	6.475105301399964E-4
48-49	0.0	0.0	0.0	0.8250787303428522	6.706359062164249E-4
50-51	0.0	0.0	0.0	1.0116311391514006	6.937612822928533E-4
52-53	0.0	0.0	0.0	1.1964607074422549	7.400120344457101E-4
54-55	0.0	0.0	0.0	1.4007849677655382	7.631374105221386E-4
56-57	0.0	0.0	0.0	1.6511518518569908	7.978254746367813E-4
58-59	0.0	0.0	0.0	1.9512498572008028	8.325135387514239E-4
60-61	0.0	0.0	0.0	2.260748327919683	8.325135387514239E-4
62-63	0.0	0.0	0.0	2.5901461847523297	8.556389148278524E-4
64-65	0.0	0.0	0.0	2.9331417627179164	8.787642909042808E-4
66-67	0.0	0.0	0.0	3.3272444218124098	9.250150430571377E-4
68-69	0.0	0.0	0.0	3.7696097407784093	9.712657952099946E-4
70-71	0.0	0.0	0.0	4.260526786816871	9.712657952099946E-4
72-73	0.0	0.0	0.0	4.7587977024476364	0.0010059538593246372
74-75	0.0	0.0	0.0	5.24878973344304	0.0010637672995157084
76-77	0.0	0.0	0.0	5.8075681955777805	0.0011100180516685652
78-79	0.0	0.0	0.0	6.420864731812701	0.0011215807397067794
80-81	0.0	0.0	0.0	7.071774692235932	0.001144706115783208
82-83	0.0	0.0	0.0	7.7439137478973255	0.0012372076200889218
84-85	0.0	0.0	0.0	8.398662520749244	0.001248770308127136
86-87	0.0	0.0	0.0	9.086734960527295	0.0012950210602799927
88	0.0	0.0	0.0	9.650196311317513	0.0013412718124328497
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6545	0.0	40.303207	94
GTACTAG	6895	0.0	34.849247	1
TAGTACT	8390	0.0	33.440903	4
CTGTGCG	8475	0.0	33.321926	9
ACTGTGC	9200	0.0	31.411425	8
TAGGCAT	8050	0.0	31.288834	5
GCATCGT	7205	0.0	30.521946	8
GTACTGT	9345	0.0	30.270357	6
TACTGTG	10190	0.0	29.143604	7
AGGCATC	7710	0.0	29.071295	6
CTAGTAC	10390	0.0	28.542665	3
TACCCCG	400	0.0	28.194973	5
CTAGGCA	9295	0.0	27.960272	4
TCTAACG	3125	0.0	27.822903	2
AAGCTAA	2740	0.0	27.781761	9
CGTTAAC	915	0.0	27.751053	1
CTAACGC	3240	0.0	26.545248	3
GTCTAAC	3360	0.0	26.310274	1
TAACGCC	3320	0.0	25.621593	4
CGCCTAC	3300	0.0	25.488216	7
>>END_MODULE