FastQCFastQC Report
Mon 16 Jan 2017
ERR1146683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324254
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC61600.040.9940894
CTGTGCG86050.031.5211339
TAGTACT86950.030.716464
ACTGTGC94200.030.0353058
TAGGCAT81600.029.8343125
GTACTGT93150.028.6577386
TACTGTG102650.028.1119447
CTAGGCA93950.027.2767184
CTAACGC30650.027.0006733
TCTAACG30800.026.8719752
TAACGCC30950.026.7389564
CTAGTAC105350.026.6906433
CGCCTAC31100.026.4459467
AGGCATC75500.025.4596736
GTATCAA157050.025.0625441
GTCTAAC34150.024.7867411
CAACGCA143050.024.3119775
CAGTACG8850.023.9026539
AAGCTAA31200.023.8056149
CGCAAGT54650.023.73533476-77
Produced by FastQC (version 0.11.5)