FastQCFastQC Report
Mon 16 Jan 2017
ERR1146682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834952
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG46800.035.16261
GGGCACC47950.033.9960194
GTATCAA94950.032.9295231
CAACGCA87250.031.8320945
GCATCGT49150.031.0721658
TAGGCAT58150.030.7906175
ATCAACG92100.030.3627363
GGTATCA63150.029.111341
CTAGGCA67250.027.9541824
CTGTGCG63300.027.7638449
TAGTACT63550.027.2891434
TATCAAC115750.026.7579292
AACGCAG111800.026.2284416
ACGCAGA108400.025.3598847
ACTGTGC73400.025.1598598
GTACATG191400.024.8597851
CGCAGAG109100.024.8520818
CGACGAG29800.024.8370924-25
CTAGTAC72850.024.644353
CATCGTC63200.024.4619229
Produced by FastQC (version 0.11.5)