##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146682_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4834952
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36807252688341	31.0	31.0	34.0	28.0	34.0
2	31.502541493690114	33.0	31.0	34.0	28.0	34.0
3	31.6260695038958	33.0	31.0	34.0	28.0	34.0
4	35.039809909178004	37.0	35.0	37.0	32.0	37.0
5	35.2027331398533	37.0	35.0	37.0	32.0	37.0
6	35.272596294647805	37.0	35.0	37.0	32.0	37.0
7	35.20377037869249	37.0	35.0	37.0	32.0	37.0
8	35.16271019857074	37.0	35.0	37.0	32.0	37.0
9	36.67113696268339	39.0	37.0	39.0	32.0	39.0
10-11	36.51354077558578	39.0	37.0	39.0	32.0	39.0
12-13	36.43810321177956	39.0	37.0	39.0	32.0	39.0
14-15	37.63504860027565	40.0	38.0	41.0	31.5	41.0
16-17	37.65834469504558	40.0	38.0	41.0	31.5	41.0
18-19	37.63789692224452	40.0	37.5	41.0	31.0	41.0
20-21	37.70722656605484	40.0	38.0	41.0	31.0	41.0
22-23	37.65957924711559	40.0	37.0	41.0	31.0	41.0
24-25	37.62926157281396	40.0	37.0	41.0	31.0	41.0
26-27	37.67394536698606	40.0	37.0	41.0	31.0	41.0
28-29	37.66268920560121	40.0	37.0	41.0	31.0	41.0
30-31	37.535325686790685	40.0	37.0	41.0	31.0	41.0
32-33	37.526533872518286	40.0	37.0	41.0	31.0	41.0
34-35	37.46025369021244	40.0	37.0	41.0	31.0	41.0
36-37	37.34707748908366	40.0	36.0	41.0	30.0	41.0
38-39	37.24256476589633	40.0	36.0	41.0	30.0	41.0
40-41	37.165656556673156	40.0	36.0	41.0	30.0	41.0
42-43	37.006066968193274	40.0	36.0	41.0	30.0	41.0
44-45	36.86327134168033	39.5	35.0	41.0	30.0	41.0
46-47	36.86118093830094	39.0	35.0	41.0	30.0	41.0
48-49	36.73302640853518	39.0	35.0	41.0	30.0	41.0
50-51	36.569472251224006	39.0	35.0	41.0	29.5	41.0
52-53	36.36779413735648	39.0	35.0	41.0	29.0	41.0
54-55	36.15903446404432	38.5	35.0	40.5	29.0	41.0
56-57	35.92605531554398	38.0	34.5	40.0	28.5	41.0
58-59	35.64882567603567	37.5	34.0	40.0	28.0	41.0
60-61	35.39564984305946	37.0	34.0	40.0	28.0	41.0
62-63	35.09386370330047	36.5	34.0	40.0	28.0	41.0
64-65	34.94143437204754	36.0	34.0	39.5	28.0	41.0
66-67	34.67888853912096	35.5	33.5	39.0	27.5	41.0
68-69	34.54843791623991	35.0	33.5	39.0	28.0	41.0
70-71	34.43002991549864	35.0	34.0	39.0	28.0	41.0
72-73	34.192425178161024	35.0	33.0	38.0	27.5	40.5
74-75	33.92802296692915	35.0	33.0	37.0	27.0	40.0
76-77	33.65454744948864	35.0	33.0	37.0	27.0	39.0
78-79	33.39679752766936	35.0	33.0	36.0	26.0	39.0
80-81	33.19086280484274	35.0	33.0	36.0	26.0	38.5
82-83	32.93701664463266	35.0	33.0	36.0	26.0	37.0
84-85	32.7480868476047	35.0	33.0	35.0	26.0	37.0
86-87	32.56946232351427	35.0	33.0	35.0	26.0	36.5
88-89	32.43171845346138	35.0	33.0	35.0	25.5	36.0
90-91	32.28467852421286	35.0	32.0	35.0	25.0	36.0
92-93	32.140723630762	35.0	32.0	35.0	25.0	36.0
94-95	32.06226587151227	35.0	32.0	35.0	25.0	35.5
96-97	31.957907958548503	35.0	32.0	35.0	25.0	35.0
98-99	31.86527611856333	35.0	32.0	35.0	25.0	35.0
100	30.93117201577182	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3661441535736323
1101	2	0.4798247257885073
1101	3	0.49756698191470505
1101	4	0.5875496527823785
1101	5	0.4762502586788031
1101	6	0.4515314776285777
1101	7	0.4803939266689028
1101	8	0.4785902924175005
1101	9	0.6372739984756777
1101	10-11	0.6655315133581823
1101	12-13	0.5460598879139056
1101	14-15	0.7432163149406534
1101	16-17	0.6974021876618579
1101	18-19	0.6760176195050249
1101	20-21	0.7072189248939793
1101	22-23	0.6692057988889033
1101	24-25	0.744463437919265
1101	26-27	0.6873454706511524
1101	28-29	0.7221989819565877
1101	30-31	0.6886138081924287
1101	32-33	0.6873067319523614
1101	34-35	0.760974567625432
1101	36-37	0.6938623947983231
1101	38-39	0.7243805869617219
1101	40-41	0.6325059352747715
1101	42-43	0.7022450701131007
1101	44-45	0.7027721371765168
1101	46-47	0.639644512926651
1101	48-49	0.6317962047534706
1101	50-51	0.6299060154959975
1101	52-53	0.6464524577744086
1101	54-55	0.6651828993817972
1101	56-57	0.5576305923092306
1101	58-59	0.574909597221577
1101	60-61	0.583125601343383
1101	62-63	0.5918928598695601
1101	64-65	0.6437068635502001
1101	66-67	0.6580250753479007
1101	68-69	0.6452588204495129
1101	70-71	0.6868764478414349
1101	72-73	0.613378897785644
1101	74-75	0.5546656343569296
1101	76-77	0.5570354628628778
1101	78-79	0.531893781553805
1101	80-81	0.46664998092278864
1101	82-83	0.5328309550738695
1101	84-85	0.48463792405043193
1101	86-87	0.4974199722135495
1101	88-89	0.5176941510577322
1101	90-91	0.5110277066089708
1101	92-93	0.5379448738221839
1101	94-95	0.5649229941170049
1101	96-97	0.546899763464264
1101	98-99	0.5353290193638287
1101	100	0.5614644893832121
1102	1	0.3293732144521222
1102	2	0.39808583312758117
1102	3	0.4130189194096623
1102	4	0.5229706476933842
1102	5	0.3742599241942557
1102	6	0.4384169126311406
1102	7	0.38034501571529944
1102	8	0.4504399159601107
1102	9	0.5794528741439962
1102	10-11	0.6598457591910787
1102	12-13	0.5728212708202989
1102	14-15	0.7411529024046075
1102	16-17	0.6875891073052145
1102	18-19	0.659687629696613
1102	20-21	0.6602227883203682
1102	22-23	0.7605000178940529
1102	24-25	0.7459572028363795
1102	26-27	0.7327049860157473
1102	28-29	0.6839698761666355
1102	30-31	0.7374258147394812
1102	32-33	0.6534120840474102
1102	34-35	0.6970701138921456
1102	36-37	0.7486496879349147
1102	38-39	0.676156069705641
1102	40-41	0.6042120123071086
1102	42-43	0.6219250489489241
1102	44-45	0.6509838144799502
1102	46-47	0.611197805868656
1102	48-49	0.5697294999703573
1102	50-51	0.5862895000643746
1102	52-53	0.5078260036710418
1102	54-55	0.46174304014095924
1102	56-57	0.4728979011185075
1102	58-59	0.4460564555508526
1102	60-61	0.4850668641385454
1102	62-63	0.5371606045564619
1102	64-65	0.5025902150388006
1102	66-67	0.48990709376955266
1102	68-69	0.4194536925430654
1102	70-71	0.4459235607289216
1102	72-73	0.3919350517300515
1102	74-75	0.36206438759564463
1102	76-77	0.37388864764862006
1102	78-79	0.3695066168923802
1102	80-81	0.4275951281345485
1102	82-83	0.3903642772565519
1102	84-85	0.41602524500376603
1102	86-87	0.431529438766475
1102	88-89	0.4145108072182708
1102	90-91	0.41776960833833243
1102	92-93	0.3884216776156961
1102	94-95	0.47092312856776175
1102	96-97	0.46196333493963593
1102	98-99	0.4196167844490475
1102	100	0.5430811944830651
1103	1	0.27128091210271776
1103	2	0.37790789908286726
1103	3	0.37434770020467667
1103	4	0.45936544768721177
1103	5	0.27741091087330005
1103	6	0.36525848610901335
1103	7	0.33592977402227575
1103	8	0.39967337046479656
1103	9	0.44740481745604654
1103	10-11	0.5525117392871124
1103	12-13	0.5647157911479752
1103	14-15	0.6533746457036429
1103	16-17	0.6204530754707136
1103	18-19	0.6163678617494455
1103	20-21	0.5664733937199884
1103	22-23	0.6288255206784967
1103	24-25	0.6490496793221752
1103	26-27	0.6107605854066094
1103	28-29	0.5715400202839547
1103	30-31	0.5693651213806561
1103	32-33	0.5416489946902487
1103	34-35	0.6247527725370077
1103	36-37	0.605933266603131
1103	38-39	0.6232679305701723
1103	40-41	0.6195750978079317
1103	42-43	0.6267710616200404
1103	44-45	0.5567832143463392
1103	46-47	0.5320169937712365
1103	48-49	0.5367875321860893
1103	50-51	0.5091877999810421
1103	52-53	0.5144615753426081
1103	54-55	0.4915181038492733
1103	56-57	0.4908962962935277
1103	58-59	0.5459248809702046
1103	60-61	0.5765134848819216
1103	62-63	0.48506449898071224
1103	64-65	0.4474518436713524
1103	66-67	0.4933917261461147
1103	68-69	0.4917419838287316
1103	70-71	0.47020706782899424
1103	72-73	0.4558554194755047
1103	74-75	0.4503851096936202
1103	76-77	0.3536548186458859
1103	78-79	0.41648652045890344
1103	80-81	0.3545766212538055
1103	82-83	0.3760661118996609
1103	84-85	0.43422062115487847
1103	86-87	0.3982769778620039
1103	88-89	0.41660814386816725
1103	90-91	0.45090674374742434
1103	92-93	0.4064335499088969
1103	94-95	0.3360900012608994
1103	96-97	0.3784335871126494
1103	98-99	0.4060318037650603
1103	100	0.6168531169202573
1104	1	0.22797780008444235
1104	2	0.35625379932675116
1104	3	0.32073609802884206
1104	4	0.5109286397965178
1104	5	0.3885539759528598
1104	6	0.3762436307511834
1104	7	0.3845569306772276
1104	8	0.4498991564706074
1104	9	0.5062553821580877
1104	10-11	0.5745492066166449
1104	12-13	0.4951519870095922
1104	14-15	0.6143890549985471
1104	16-17	0.5204255265130584
1104	18-19	0.5452472933546986
1104	20-21	0.5437174779651173
1104	22-23	0.5864588277400813
1104	24-25	0.5804799643874503
1104	26-27	0.5464840927754651
1104	28-29	0.49698292087558826
1104	30-31	0.46492659953815263
1104	32-33	0.5333585408372272
1104	34-35	0.6080444640207716
1104	36-37	0.500636496982132
1104	38-39	0.5056374479571559
1104	40-41	0.5295053932705471
1104	42-43	0.5348519996642693
1104	44-45	0.4765155719623735
1104	46-47	0.4750578116780275
1104	48-49	0.4650903159186939
1104	50-51	0.3890058768894704
1104	52-53	0.45756836168065007
1104	54-55	0.5297634819721964
1104	56-57	0.5253405871051413
1104	58-59	0.46746570038011726
1104	60-61	0.4483569950620563
1104	62-63	0.4637540274221408
1104	64-65	0.41720731469742134
1104	66-67	0.4391247800372824
1104	68-69	0.4853781425907684
1104	70-71	0.5002974154957016
1104	72-73	0.45531812852726006
1104	74-75	0.4324340295768536
1104	76-77	0.42245542753709486
1104	78-79	0.38214771509889545
1104	80-81	0.39150013147592944
1104	82-83	0.3748225220308612
1104	84-85	0.4491088876025202
1104	86-87	0.43967597964232397
1104	88-89	0.3875093149754818
1104	90-91	0.40445558474782217
1104	92-93	0.4207662655742155
1104	94-95	0.520423266411143
1104	96-97	0.49685444570978277
1104	98-99	0.38671748872933165
1104	100	0.48839707865817417
1105	1	0.08971516272806923
1105	2	0.23254180956271497
1105	3	0.18796752320517385
1105	4	0.21390347530955722
1105	5	0.16709061250825386
1105	6	0.2343306127836513
1105	7	0.21373317830096283
1105	8	0.2706970343295083
1105	9	0.37864609647858316
1105	10-11	0.34204041059109613
1105	12-13	0.30263317985406957
1105	14-15	0.33779947198009097
1105	16-17	0.3328071625313811
1105	18-19	0.34012812680206395
1105	20-21	0.30327861015710056
1105	22-23	0.3630984700737443
1105	24-25	0.30815616259330625
1105	26-27	0.3915120995099315
1105	28-29	0.39515934148482046
1105	30-31	0.41879607464328217
1105	32-33	0.4121373845738958
1105	34-35	0.3990098489801994
1105	36-37	0.40492932654739633
1105	38-39	0.39122310318411024
1105	40-41	0.38986877753243476
1105	42-43	0.38571052837417596
1105	44-45	0.40065449630268546
1105	46-47	0.39472006057118847
1105	48-49	0.29597562686621615
1105	50-51	0.33206283313759144
1105	52-53	0.37387222888966676
1105	54-55	0.33433103573977263
1105	56-57	0.3943689336894849
1105	58-59	0.4391170267943636
1105	60-61	0.45522819732809694
1105	62-63	0.40170652794037665
1105	64-65	0.37762147484753683
1105	66-67	0.34580439340086144
1105	68-69	0.2762713387738671
1105	70-71	0.31208484570153416
1105	72-73	0.3263980823345065
1105	74-75	0.3445231920072658
1105	76-77	0.2758477108226387
1105	78-79	0.3559413291283633
1105	80-81	0.334715723430584
1105	82-83	0.32693361672738064
1105	84-85	0.21799200261108354
1105	86-87	0.2116217937461613
1105	88-89	0.2633007730914443
1105	90-91	0.29778265695560435
1105	92-93	0.3287010088263713
1105	94-95	0.25508110343197643
1105	96-97	0.20726599889026076
1105	98-99	0.2311052530414628
1105	100	0.3075308210392471
1106	1	0.09238747196584995
1106	2	0.21581946238879723
1106	3	0.1818464792749026
1106	4	0.26148515683726004
1106	5	0.23960135946818184
1106	6	0.20254080197982915
1106	7	0.22921255210678737
1106	8	0.19614278676490216
1106	9	0.33945686284123866
1106	10-11	0.3455794962011325
1106	12-13	0.24053998422306933
1106	14-15	0.3611847927924714
1106	16-17	0.340082015542599
1106	18-19	0.35455613728471036
1106	20-21	0.3404072645018772
1106	22-23	0.3605271914270958
1106	24-25	0.40579733297934695
1106	26-27	0.3742066861073283
1106	28-29	0.3671224207193333
1106	30-31	0.43266457570810246
1106	32-33	0.3229629275614627
1106	34-35	0.32899775009665433
1106	36-37	0.33230979634438995
1106	38-39	0.3199709227947025
1106	40-41	0.32410151928099395
1106	42-43	0.298888254087494
1106	44-45	0.3377219398565714
1106	46-47	0.23604637512342208
1106	48-49	0.19096810987028334
1106	50-51	0.20491196665392408
1106	52-53	0.1845292984881297
1106	54-55	0.2624818659916599
1106	56-57	0.2874568397440811
1106	58-59	0.273956314231782
1106	60-61	0.23333206602271872
1106	62-63	0.255045954635456
1106	64-65	0.2131101303884364
1106	66-67	0.19447957154177686
1106	68-69	0.1804902950415439
1106	70-71	0.23213455901075264
1106	72-73	0.24652681643842556
1106	74-75	0.2573825315998022
1106	76-77	0.2858925723077945
1106	78-79	0.2899657161586049
1106	80-81	0.2351892786162182
1106	82-83	0.2769275701862597
1106	84-85	0.20610959560919184
1106	86-87	0.21041995689856208
1106	88-89	0.35229263845000247
1106	90-91	0.27302817561952963
1106	92-93	0.2447680213433472
1106	94-95	0.26010040059131967
1106	96-97	0.27687056494558604
1106	98-99	0.3047978750887239
1106	100	0.274220593890238
1107	1	0.09085325017140633
1107	2	0.1445989465916817
1107	3	0.11532570303176826
1107	4	0.2342579284218118
1107	5	0.1148685773200242
1107	6	0.19566765653340923
1107	7	0.14146956256632848
1107	8	0.2042051686688069
1107	9	0.21162374624920943
1107	10-11	0.2670573762161439
1107	12-13	0.20175539229225592
1107	14-15	0.30933638810870434
1107	16-17	0.2502282511187346
1107	18-19	0.1648499342521248
1107	20-21	0.22988356267511278
1107	22-23	0.3234074647824272
1107	24-25	0.3524616862468264
1107	26-27	0.25355546619647384
1107	28-29	0.30008106044337524
1107	30-31	0.401402738845384
1107	32-33	0.36768211690319674
1107	34-35	0.34750338339210884
1107	36-37	0.3281787020547853
1107	38-39	0.3626823725737296
1107	40-41	0.31475792271031366
1107	42-43	0.3714870500701579
1107	44-45	0.36856289703073486
1107	46-47	0.29398650413551053
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2216	100	-0.7885802852286368
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	2.0
2	9.0
3	31.0
4	38.0
5	28.0
6	49.0
7	178.0
8	448.0
9	835.0
10	1241.0
11	2369.0
12	3996.0
13	5900.0
14	8434.0
15	11057.0
16	13521.0
17	15969.0
18	18182.0
19	20778.0
20	23652.0
21	27059.0
22	31128.0
23	36112.0
24	41092.0
25	48172.0
26	54026.0
27	59516.0
28	68159.0
29	83063.0
30	97889.0
31	112755.0
32	140483.0
33	183066.0
34	262150.0
35	380257.0
36	605351.0
37	1078215.0
38	1016449.0
39	382337.0
40	956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7922468033202	20.54728325417213	12.72492442762736	23.935545514880314
2	15.64053271511705	19.724818463088837	40.74280688559896	23.89184193619515
3	18.557910110821	26.32241284768209	29.07391851945775	26.045758522039165
4	12.159018089587038	16.728679361273382	39.81196377531961	31.30033877381997
5	13.27666619163673	38.352683046995075	35.07023996733669	13.3004107940315
6	30.257209268270284	38.230539391555176	18.12527379520801	13.386977544966532
7	26.9558760720221	31.870346987169345	23.549480289685114	17.624296651123444
8	25.889855520895427	35.27731574229465	20.110132896618452	18.722695840191474
9	26.047498151470812	15.395316369954031	21.26717580934554	37.29000966922962
10-11	25.41243336902842	25.935967048143898	27.918042289111582	20.733557293716096
12-13	25.640674564312338	23.88240150918015	27.662694265475313	22.814229661032197
14-15	23.053294613826488	25.230206882416127	25.06889169048373	26.647606813273654
16-17	22.289205841656447	27.76321477249929	26.23618128907761	23.711398096766654
18-19	21.801760976058226	28.26035541142683	27.719038473689757	22.218845138825188
20-21	23.43647360940362	27.430120639417037	26.812404134601227	22.321001616578116
22-23	23.772076256559355	27.0639098436386	26.531208886077838	22.6328050137242
24-25	23.201158661313908	27.157515476050126	27.189284554560704	22.45204130807526
26-27	23.076372578392164	27.44783447680178	26.327741798305755	23.1480511465003
28-29	22.290095298432114	27.86200637280924	26.885535878258267	22.962362450500382
30-31	23.014237767858535	27.43376724753897	26.89304211843871	22.65895286616378
32-33	23.32801445229849	27.704813375325916	26.010103908989734	22.95706826338586
34-35	23.375318706397955	27.451409878300538	26.83313963264919	22.34013178265232
36-37	23.063904585820772	27.117813708781398	26.582613229149764	23.235668476248065
38-39	23.16204386567474	27.21100487049352	27.064116478161793	22.562834785669946
40-41	22.8114009327893	26.53368467286961	27.633011727141188	23.0219026671999
42-43	22.805046953054806	26.749097351012622	27.045131259937566	23.400724435995002
44-45	23.036959031833216	27.554093584339384	26.84544071057263	22.56350667325478
46-47	22.891904602962796	27.36526591215088	26.45817962766	23.284649857226327
48-49	23.4805825202549	26.914575253232798	26.74111254450865	22.86372968200365
50-51	23.131997951613435	26.886966483683395	26.676056311805723	23.304979252897443
52-53	22.673247806240834	27.419806972364096	26.51737422502623	23.389570996368835
54-55	22.64731107967651	27.272724451812593	26.56382200991224	23.516142458598658
56-57	23.57555289810598	26.792376994871343	26.40645036240676	23.225619744615926
58-59	22.66610206551759	27.113773820145298	27.067663806908058	23.15246030742905
60-61	22.688601413710956	27.660037485339995	26.502131240475972	23.14922986047307
62-63	23.04470111551108	27.08273001520775	26.18520983887062	23.687359030410544
64-65	22.363271119506543	27.195653295860033	26.618339845549855	23.82273573908357
66-67	22.9777903500475	26.89691158287002	26.573791609802715	23.551506457279768
68-69	22.516091040602912	26.94661361108227	26.759569096432067	23.777726251882754
70-71	22.458180038549976	26.62333370944917	26.920241137481852	23.998245114518998
72-73	22.88022187152595	26.81344405259727	27.217549163984472	23.0887849118923
74-75	23.090834643134954	26.975631949761535	26.84191272785868	23.09162067924483
76-77	22.757162970315317	26.90984530853123	26.62530294422537	23.707688776928073
78-79	22.65767558958565	26.83573598386629	26.708479953400133	23.798108473147934
80-81	22.30912792582775	27.192037932141844	27.20245282657856	23.296381315451846
82-83	22.979946895439202	26.279724974884232	28.08427479097086	22.656053338705703
84-85	23.146470076281165	26.796713357917472	26.91092566994886	23.1458908958525
86-87	22.06462058030422	27.30608235857351	27.34788622109734	23.281410840024932
88-89	23.010122388293713	26.25530790887472	27.139147520204133	23.595422182627434
90-91	22.6644116649589	26.3575699474411	27.270982163773567	23.70703622382643
92-93	22.878198633097295	26.44633945574383	27.285163439122485	23.390298472036392
94-95	21.922394618182015	26.408084103368353	27.872825209333314	23.796696069116322
96-97	22.206797122126563	26.356939666116624	27.466512634005053	23.96975057775176
98-99	21.61882024517137	27.017821506109428	27.3464803776857	24.016877871033508
100	22.378677348093564	26.38236685318105	26.639027175054697	24.59992862367069
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1123.0
1	782.0
2	544.0
3	638.0
4	620.0
5	544.5
6	476.5
7	486.0
8	515.0
9	510.0
10	441.0
11	405.5
12	419.5
13	452.0
14	503.5
15	714.5
16	872.0
17	1008.5
18	1345.0
19	1761.0
20	2309.0
21	3010.0
22	4248.5
23	6043.0
24	7813.0
25	10004.5
26	13534.0
27	17491.0
28	22441.0
29	31475.5
30	43832.0
31	55811.0
32	71508.5
33	86537.5
34	96659.0
35	108295.0
36	119622.0
37	139607.5
38	148982.5
39	141994.0
40	135780.0
41	128394.5
42	128345.0
43	136882.0
44	148884.0
45	172922.0
46	196278.0
47	212293.0
48	222569.5
49	217602.5
50	207363.5
51	210468.0
52	211778.5
53	202647.5
54	193902.0
55	171430.5
56	151649.0
57	127992.5
58	103386.0
59	97757.0
60	82219.0
61	59705.5
62	46525.0
63	38522.5
64	28630.5
65	16327.5
66	10250.5
67	7597.0
68	5657.5
69	4164.0
70	3244.5
71	2396.5
72	1619.5
73	1282.0
74	1089.0
75	849.5
76	606.0
77	357.0
78	222.0
79	135.0
80	77.0
81	50.0
82	44.0
83	37.5
84	28.5
85	22.5
86	19.0
87	12.5
88	11.5
89	13.5
90	12.0
91	12.0
92	10.5
93	8.0
94	9.0
95	7.5
96	4.0
97	3.5
98	3.5
99	3.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05851143920353294
2	0.008231725981974589
3	0.008252408710572515
4	0.009472689697850154
5	0.003495381133049511
6	0.0027714856321220975
7	9.720882441025268E-4
8	0.0013650600874631227
9	5.584336721440047E-4
10-11	4.0331320765955896E-4
12-13	1.8614455738133492E-4
14-15	1.5512046448444574E-4
16-17	4.550200291543742E-4
18-19	0.0015822287377413467
20-21	9.92770972700453E-4
22-23	0.0013030119016693444
24-25	0.0012823291730714183
26-27	0.0035884534117401785
28-29	0.003981425255100774
30-31	0.004974196227801228
32-33	0.0059669672005016805
34-35	0.003578112047441215
36-37	0.008448894632252811
38-39	0.006804617708717688
40-41	0.006463352686851907
42-43	0.014653713211630642
44-45	0.02050692540484373
46-47	0.01866616255962831
48-49	0.021923692313801667
50-51	0.01868684528822623
52-53	0.025646583461428365
54-55	0.019234937596071276
56-57	0.015367267348259094
58-59	0.017807829322814373
60-61	0.016453110599650215
62-63	0.011913251672405435
64-65	0.011923593036704398
66-67	0.014053914082290784
68-69	0.005667067635831751
70-71	0.009276203776169858
72-73	0.0091521074045823
74-75	0.01175813120792099
76-77	0.010806725692416388
78-79	0.012482026708848402
80-81	0.01095150479260187
82-83	0.007011444994696948
84-85	0.0110549184355915
86-87	0.009731223805324232
88-89	0.008707428739726889
90-91	0.013681624967528115
92-93	0.009214155590376078
94-95	0.0078077300457171026
96-97	0.009917368362705566
98-99	0.009431324240654302
100	0.261119448548817
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4834952.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.41257160523276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50810008086542	49.48274531790322
2	15.249135531372172	20.254686107903062
3	5.227111317275581	10.41437713941261
4	2.2666997066916474	6.021494263128764
5	1.07021539275215	3.5537878202087225
6	0.5820022240124633	2.3191358625979457
7	0.3321951707265482	1.5443354893952579
8	0.20640720154977937	1.0966426442208344
9	0.14055183453285475	0.8400967897644036
>10	0.4108148724391296	3.9321701137003457
>50	0.0058725810679410755	0.25599989719198174
>100	8.05788488214213E-4	0.10101116144522763
>500	0.0	0.0
>1k	8.829822602396905E-5	0.18351739312762536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.06827285979261E-5	6.204818579377831E-5
2	0.0	0.0	0.0	2.06827285979261E-5	6.204818579377831E-5
3	0.0	0.0	0.0	2.06827285979261E-5	6.204818579377831E-5
4	0.0	0.0	0.0	2.06827285979261E-5	6.204818579377831E-5
5	0.0	0.0	0.0	2.06827285979261E-5	1.0341364298963051E-4
6	0.0	0.0	0.0	2.06827285979261E-5	1.2409637158755662E-4
7	0.0	0.0	0.0	2.06827285979261E-5	1.2409637158755662E-4
8	0.0	0.0	0.0	2.06827285979261E-5	1.2409637158755662E-4
9	0.0	0.0	0.0	2.06827285979261E-5	1.2409637158755662E-4
10-11	0.0	0.0	0.0	6.204818579377831E-5	1.447791001854827E-4
12-13	0.0	0.0	0.0	6.204818579377831E-5	3.826304790616329E-4
14-15	0.0	0.0	0.0	6.204818579377831E-5	4.860441220512634E-4
16-17	0.0	0.0	0.0	7.238955009274137E-5	5.480923078450417E-4
18-19	0.0	0.0	0.0	8.27309143917044E-5	5.791164007419308E-4
20-21	0.0	0.0	0.0	8.27309143917044E-5	6.1014049363882E-4
22-23	0.0	0.0	0.0	1.8614455738133492E-4	7.238955009274135E-4
24-25	0.0	0.0	0.0	3.4126502186578066E-4	7.859436867211918E-4
26-27	0.0	0.0	0.0	7.032127723294874E-4	8.169677796180811E-4
28-29	0.0	0.0	0.0	0.002409537881658391	8.479918725149702E-4
30-31	0.0	0.0	0.0	0.008955621482902002	8.686746011128963E-4
32-33	0.0	0.0	0.0	0.021716865027822406	9.92770972700453E-4
34-35	0.0	0.0	0.0	0.04110692308837813	0.001013453701298379
36-37	0.0	0.0	0.0	0.07472669842430701	0.001013453701298379
38-39	0.0	0.0	0.0	0.12288643196457794	0.0010341364298963051
40-41	0.0	0.0	0.0	0.17594797218255737	0.0010548191584942312
42-43	0.0	0.0	0.0	0.23066413068837086	0.0010755018870921573
44-45	0.0	0.0	0.0	0.29996161285572226	0.0011375500728859355
46-47	0.0	0.0	0.0	0.38789423348980506	0.0011892568943807507
48-49	0.0	0.0	0.0	0.4887845835904886	0.0012513050801745292
50-51	0.0	0.0	0.0	0.5957763386275603	0.0013030119016693444
52-53	0.0	0.0	0.0	0.6989107647811188	0.0014788150947517163
54-55	0.0	0.0	0.0	0.8155406713448241	0.0015615460091434206
56-57	0.0	0.0	0.0	0.9619020002680483	0.0015822287377413467
58-59	0.0	0.0	0.0	1.1389461570662955	0.0015925701020403098
60-61	0.0	0.0	0.0	1.3250079835332387	0.0016132528306382358
62-63	0.0	0.0	0.0	1.5208320578984031	0.0016959837450299404
64-65	0.0	0.0	0.0	1.7269044242838398	0.0016959837450299404
66-67	0.0	0.0	0.0	1.9629046989504757	0.0017063251093289034
68-69	0.0	0.0	0.0	2.234686094091524	0.0017166664736278665
70-71	0.0	0.0	0.0	2.5343064419253802	0.0017373492022257925
72-73	0.0	0.0	0.0	2.849356105293289	0.001871786938112312
74-75	0.0	0.0	0.0	3.160651853420675	0.001975200581101943
76-77	0.0	0.0	0.0	3.519838459616559	0.002026907402596758
78-79	0.0	0.0	0.0	3.9167607041393584	0.002047590131194684
80-81	0.0	0.0	0.0	4.347830133577334	0.0020786142240915733
82-83	0.0	0.0	0.0	4.803098355474884	0.00220271059567913
84-85	0.0	0.0	0.0	5.250827722798489	0.002213051959978093
86-87	0.0	0.0	0.0	5.724875862262955	0.002254417417173945
88	0.0	0.0	0.0	6.1087679877690615	0.0023164656029677233
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4680	0.0	35.1626	1
GGGCACC	4795	0.0	33.99601	94
GTATCAA	9495	0.0	32.929523	1
CAACGCA	8725	0.0	31.832094	5
GCATCGT	4915	0.0	31.072165	8
TAGGCAT	5815	0.0	30.790617	5
ATCAACG	9210	0.0	30.362736	3
GGTATCA	6315	0.0	29.11134	1
CTAGGCA	6725	0.0	27.954182	4
CTGTGCG	6330	0.0	27.763844	9
TAGTACT	6355	0.0	27.289143	4
TATCAAC	11575	0.0	26.757929	2
AACGCAG	11180	0.0	26.228441	6
ACGCAGA	10840	0.0	25.359884	7
ACTGTGC	7340	0.0	25.159859	8
GTACATG	19140	0.0	24.859785	1
CGCAGAG	10910	0.0	24.852081	8
CGACGAG	2980	0.0	24.83709	24-25
CTAGTAC	7285	0.0	24.64435	3
CATCGTC	6320	0.0	24.461922	9
>>END_MODULE