FastQCFastQC Report
Mon 16 Jan 2017
ERR1146682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834952
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142280.29427386249129256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121130.25052989150667887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81570.1687090171732832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.047.8624151
GTATCAA178750.041.6082531
GGGCACC43350.035.0413894
GTGGTAT27300.034.118991
CAACGCA218350.032.6528935
ATCAACG219950.032.4645963
TATCAAC238900.031.0337122
AACGCAG246600.029.635566
GTACTAG43600.029.2399391
TCAACGC254650.028.2439864
CTGTGCG60400.027.2376619
ACGCAGA260750.027.1982357
TAGGCAT54850.026.9912625
CGCAGAG264000.026.8458848
TAGTACT61050.026.6595384
CTAACGC18950.025.8158443
TAACGCC19800.025.1827344
CTAGTAC69250.025.0650623
TCTAACG20050.024.8705372
CAGTACG8000.024.6773199
Produced by FastQC (version 0.11.5)