##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4834952
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14067316490422	33.0	31.0	34.0	30.0	34.0
2	32.20285723622489	33.0	31.0	34.0	30.0	34.0
3	32.35269140210699	34.0	31.0	34.0	30.0	34.0
4	35.96335434147019	37.0	35.0	37.0	35.0	37.0
5	35.8754897670132	37.0	35.0	37.0	35.0	37.0
6	35.92097729201862	37.0	35.0	37.0	35.0	37.0
7	35.840612068123946	37.0	35.0	37.0	35.0	37.0
8	35.850082482721646	37.0	35.0	37.0	35.0	37.0
9	37.56252864557911	39.0	37.0	39.0	35.0	39.0
10-11	37.50682954039668	39.0	37.0	39.0	34.5	39.0
12-13	37.48001138377382	39.0	37.0	39.0	34.0	39.0
14-15	38.70276292298249	40.0	38.0	41.0	34.0	41.0
16-17	38.70482602516013	40.0	38.0	41.0	34.0	41.0
18-19	38.771499592963906	40.0	38.0	41.0	34.0	41.0
20-21	38.770186343111575	40.0	38.0	41.0	34.0	41.0
22-23	38.73335764243368	40.0	38.0	41.0	34.0	41.0
24-25	38.66395995244627	40.0	38.0	41.0	34.0	41.0
26-27	38.52164375158223	40.0	38.0	41.0	34.0	41.0
28-29	38.4586943158898	40.0	38.0	41.0	34.0	41.0
30-31	38.32048839368002	40.0	38.0	41.0	34.0	41.0
32-33	38.166101959233515	40.0	37.0	41.0	33.5	41.0
34-35	38.05782446237315	40.0	37.0	41.0	33.0	41.0
36-37	38.04508762444798	40.0	37.0	41.0	33.0	41.0
38-39	37.9501714805028	40.0	37.0	41.0	33.0	41.0
40-41	37.83550457171033	40.0	37.0	41.0	33.0	41.0
42-43	37.69656348191254	40.0	37.0	41.0	33.0	41.0
44-45	37.49067767373906	40.0	36.0	41.0	32.0	41.0
46-47	37.22820805666737	40.0	36.0	41.0	31.0	41.0
48-49	36.95715159116368	39.5	35.5	41.0	31.0	41.0
50-51	36.755840078660555	39.0	35.0	41.0	30.5	41.0
52-53	36.5808524055668	39.0	35.0	41.0	30.0	41.0
54-55	36.40380256101818	39.0	35.0	41.0	30.0	41.0
56-57	36.19217584786777	38.5	35.0	41.0	30.0	41.0
58-59	35.93004687533609	38.0	35.0	40.0	29.0	41.0
60-61	35.75454616715946	37.0	35.0	40.0	29.0	41.0
62-63	35.546592086126196	37.0	35.0	40.0	29.0	41.0
64-65	35.29989894418807	36.5	34.5	40.0	29.0	41.0
66-67	35.04477417769608	36.0	34.0	39.0	29.0	41.0
68-69	34.78286981959697	35.5	34.0	39.0	29.0	41.0
70-71	34.54388471695272	35.0	34.0	39.0	29.0	41.0
72-73	34.29298956845901	35.0	34.0	38.0	29.0	40.0
74-75	34.025937382625514	35.0	34.0	37.0	28.5	39.5
76-77	33.76917899081521	35.0	34.0	37.0	28.0	39.0
78-79	33.53750750783048	35.0	33.5	36.0	28.0	39.0
80-81	33.39299180219369	35.0	33.0	36.0	28.0	38.5
82-83	33.23032172811643	35.0	33.0	36.0	28.0	37.0
84-85	33.091213108217	35.0	33.0	35.0	28.0	37.0
86-87	32.93759524396519	35.0	33.0	35.0	28.0	36.5
88-89	32.83063503008923	35.0	33.0	35.0	27.5	36.0
90-91	32.760678906429675	35.0	33.0	35.0	28.0	36.0
92-93	32.694618891769764	35.0	33.0	35.0	27.0	36.0
94-95	32.64282799498319	35.0	33.0	35.0	27.0	35.5
96-97	32.5526163444849	35.0	33.0	35.0	27.0	35.0
98-99	32.47557421459406	35.0	33.0	35.0	27.0	35.0
100	31.270135049944653	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3582310593462026
1101	2	0.41298999255518964
1101	3	0.37336565312055825
1101	4	0.26495409322386365
1101	5	0.2297934530149135
1101	6	0.26317090040453195
1101	7	0.2834023741594933
1101	8	0.26167752029537894
1101	9	0.31992157657425224
1101	10-11	0.3547297601792607
1101	12-13	0.38588164034894135
1101	14-15	0.502946225433476
1101	16-17	0.5260548160980179
1101	18-19	0.4747513490736921
1101	20-21	0.48445664230872865
1101	22-23	0.47901393151990845
1101	24-25	0.5039349940425879
1101	26-27	0.600524090789115
1101	28-29	0.5956237224412249
1101	30-31	0.6314859879157098
1101	32-33	0.5962384907951375
1101	34-35	0.6308654896804953
1101	36-37	0.5975434771722945
1101	38-39	0.6299605694504322
1101	40-41	0.58980818060828
1101	42-43	0.5512144799009917
1101	44-45	0.5970104151225115
1101	46-47	0.561952450464581
1101	48-49	0.5055161220900786
1101	50-51	0.5434510361570659
1101	52-53	0.5699288721388172
1101	54-55	0.6046350268071521
1101	56-57	0.558881580868615
1101	58-59	0.5091029165940313
1101	60-61	0.5422751390792726
1101	62-63	0.5769882536698958
1101	64-65	0.522914040387505
1101	66-67	0.46957638288659354
1101	68-69	0.45948508509369645
1101	70-71	0.49241106015597325
1101	72-73	0.5562955833352135
1101	74-75	0.4859741161952442
1101	76-77	0.46941293094312186
1101	78-79	0.4162732605259549
1101	80-81	0.45324688101744215
1101	82-83	0.43102323229077655
1101	84-85	0.3889491500500952
1101	86-87	0.3718801536825751
1101	88-89	0.4267637635844679
1101	90-91	0.41552423940118643
1101	92-93	0.45200240337172914
1101	94-95	0.4447263456612518
1101	96-97	0.3921392685332208
1101	98-99	0.504522303978348
1101	100	0.7426352370823928
1102	1	0.3389414604547838
1102	2	0.3661707220744006
1102	3	0.27803244642181113
1102	4	0.1971913282733837
1102	5	0.1708068051474001
1102	6	0.17208843661239115
1102	7	0.26111839998046804
1102	8	0.2823392733006358
1102	9	0.2857642580328914
1102	10-11	0.274015009749462
1102	12-13	0.32121544082458797
1102	14-15	0.4605252060338998
1102	16-17	0.41609171252451205
1102	18-19	0.3951298667172338
1102	20-21	0.410094132247373
1102	22-23	0.4638270662430628
1102	24-25	0.42795106648639347
1102	26-27	0.4629352239924671
1102	28-29	0.5234569955616735
1102	30-31	0.5243584904952883
1102	32-33	0.5603321683665285
1102	34-35	0.5364034803867526
1102	36-37	0.48812820842631766
1102	38-39	0.5211009075798927
1102	40-41	0.4454705288086984
1102	42-43	0.4213282893456949
1102	44-45	0.4290617828752943
1102	46-47	0.45065368925526883
1102	48-49	0.46236876604717736
1102	50-51	0.4334353814372065
1102	52-53	0.46819419264644324
1102	54-55	0.47548102556872607
1102	56-57	0.4690921608194003
1102	58-59	0.4624058525844106
1102	60-61	0.43983530224689105
1102	62-63	0.46810514328497277
1102	64-65	0.3998463374351715
1102	66-67	0.4146165257031811
1102	68-69	0.47776009135057507
1102	70-71	0.3923536774810472
1102	72-73	0.34372020179809226
1102	74-75	0.3641056629282815
1102	76-77	0.3449833042368553
1102	78-79	0.4111300179954185
1102	80-81	0.39668767441684594
1102	82-83	0.29073418396669837
1102	84-85	0.33699228170129913
1102	86-87	0.3724277332238586
1102	88-89	0.33399996567019485
1102	90-91	0.2827917868558174
1102	92-93	0.33528831176954554
1102	94-95	0.3115567106931678
1102	96-97	0.3331116992260803
1102	98-99	0.4028018165102054
1102	100	0.6326357788565353
1103	1	0.26235124388760767
1103	2	0.3481561069735122
1103	3	0.2468964762594794
1103	4	0.1947872801152215
1103	5	0.19451424973434683
1103	6	0.2101313432734031
1103	7	0.22146941083996552
1103	8	0.16134106844548768
1103	9	0.16514807799346443
1103	10-11	0.2732267851386183
1103	12-13	0.2731625800497426
1103	14-15	0.3869050583636877
1103	16-17	0.3429408218892931
1103	18-19	0.3849522368416842
1103	20-21	0.34606076264762464
1103	22-23	0.3461674962070518
1103	24-25	0.3499181239627589
1103	26-27	0.444396286119499
1103	28-29	0.47466839712711106
1103	30-31	0.4036778225820683
1103	32-33	0.4422827095844468
1103	34-35	0.4487531059552339
1103	36-37	0.42985365971888
1103	38-39	0.39400224280966256
1103	40-41	0.44639117362328307
1103	42-43	0.42882454678004933
1103	44-45	0.44548507745169275
1103	46-47	0.4796127631201017
1103	48-49	0.4949257110631393
1103	50-51	0.47396889416413046
1103	52-53	0.477755602023862
1103	54-55	0.4464838531027482
1103	56-57	0.4241675041063857
1103	58-59	0.41531188102239014
1103	60-61	0.4337104759937347
1103	62-63	0.48255067304698684
1103	64-65	0.5094327947520512
1103	66-67	0.5203782151531655
1103	68-69	0.49773105731878076
1103	70-71	0.51533702812808
1103	72-73	0.46786409725312694
1103	74-75	0.43024436979305136
1103	76-77	0.4020062578558239
1103	78-79	0.39676892297109845
1103	80-81	0.38701070262378323
1103	82-83	0.37894647859396
1103	84-85	0.37795585938393117
1103	86-87	0.36962549499801867
1103	88-89	0.3038183194494124
1103	90-91	0.33179997392718974
1103	92-93	0.3300609577065501
1103	94-95	0.3329435303923418
1103	96-97	0.40935170207933425
1103	98-99	0.4117983533008953
1103	100	0.6437575997761229
1104	1	0.2415355666931589
1104	2	0.26866424515831255
1104	3	0.2260027256000825
1104	4	0.20811389937960456
1104	5	0.14488524811572034
1104	6	0.1782911200412869
1104	7	0.2437432064945071
1104	8	0.22585084757834295
1104	9	0.2800176256652662
1104	10-11	0.2634195376548263
1104	12-13	0.2541009142330992
1104	14-15	0.3356020488779805
1104	16-17	0.34813345442246657
1104	18-19	0.34598145501323074
1104	20-21	0.3751136173160816
1104	22-23	0.3768671868998723
1104	24-25	0.38279554668385884
1104	26-27	0.37456528181777315
1104	28-29	0.3842350168198365
1104	30-31	0.41132407695999973
1104	32-33	0.42875412036769234
1104	34-35	0.44933219084421694
1104	36-37	0.4201937716754074
1104	38-39	0.4149714164563818
1104	40-41	0.43930573540603035
1104	42-43	0.443387017941653
1104	44-45	0.4139658999920215
1104	46-47	0.4949459629334001
1104	48-49	0.5138704623552215
1104	50-51	0.4779456779013245
1104	52-53	0.45463625953728126
1104	54-55	0.41998339948862906
1104	56-57	0.39009336405696615
1104	58-59	0.4720741628687364
1104	60-61	0.41283832050099534
1104	62-63	0.42978838885255755
1104	64-65	0.41740709109604524
1104	66-67	0.35423804185140995
1104	68-69	0.43877751533522513
1104	70-71	0.4425259441935552
1104	72-73	0.3656111343526831
1104	74-75	0.39587239788590267
1104	76-77	0.37533895756491376
1104	78-79	0.3769836856582671
1104	80-81	0.32446572788754935
1104	82-83	0.3391564056714955
1104	84-85	0.3175263571345539
1104	86-87	0.3487360936123025
1104	88-89	0.3447083761612717
1104	90-91	0.34502817864503044
1104	92-93	0.35134970957938094
1104	94-95	0.3052114140587179
1104	96-97	0.32244166037486366
1104	98-99	0.2915028421231085
1104	100	0.5553279182904234
1105	1	0.22548557709262695
1105	2	0.1820675677003507
1105	3	0.163340778492298
1105	4	0.1925511794377286
1105	5	0.19822307734918354
1105	6	0.16058608499417915
1105	7	0.25089845391022436
1105	8	0.23346880690701255
1105	9	0.1864741117498525
1105	10-11	0.2150019077518266
1105	12-13	0.23484223523712444
1105	14-15	0.2947369245806968
1105	16-17	0.32507953160573777
1105	18-19	0.2848160459322244
1105	20-21	0.34299486243557453
1105	22-23	0.3067341046273384
1105	24-25	0.31341225453881094
1105	26-27	0.36323571245379327
1105	28-29	0.31406675649292737
1105	30-31	0.36499791479997157
1105	32-33	0.31435052419006126
1105	34-35	0.2914078708889676
1105	36-37	0.3139113212031077
1105	38-39	0.38271027246752
1105	40-41	0.38795374811903116
1105	42-43	0.26461065197156586
1105	44-45	0.28970201154679387
1105	46-47	0.29233754181483107
1105	48-49	0.29901664206158074
1105	50-51	0.3423752933770601
1105	52-53	0.29788090660389344
1105	54-55	0.2583842144832289
1105	56-57	0.15497845973933977
1105	58-59	0.23752706478698826
1105	60-61	0.29622383079693293
1105	62-63	0.318062943129938
1105	64-65	0.2609128016607585
1105	66-67	0.2718671293209596
1105	68-69	0.2739437819947028
1105	70-71	0.3054317882021351
1105	72-73	0.22396212131474158
1105	74-75	0.3125771153133954
1105	76-77	0.27137140450474107
1105	78-79	0.22030346293270497
1105	80-81	0.13910302950150566
1105	82-83	0.16909279799460109
1105	84-85	0.1645808543826135
1105	86-87	0.19040955810122284
1105	88-89	0.1505261757583085
1105	90-91	0.1603804130489337
1105	92-93	0.15378176576516012
1105	94-95	0.11446285383444632
1105	96-97	0.20731744097442117
1105	98-99	0.22467741329583646
1105	100	0.3544618875557468
1106	1	0.13024416012654427
1106	2	0.18243401674720872
1106	3	0.12861232585007798
1106	4	0.11495001660322401
1106	5	0.06886392685279219
1106	6	0.13161110213395233
1106	7	0.15618305701514146
1106	8	0.049023583603300835
1106	9	0.08350516425005594
1106	10-11	0.1469199730850903
1106	12-13	0.07934416611331585
1106	14-15	0.16099023894926745
1106	16-17	0.14426699707589563
1106	18-19	0.16545199755293538
1106	20-21	0.16533556525935467
1106	22-23	0.1849788729365187
1106	24-25	0.15067973057647777
1106	26-27	0.1843157215621929
1106	28-29	0.18506637271028836
1106	30-31	0.19417050590202223
1106	32-33	0.21594594612427187
1106	34-35	0.20310785500218742
1106	36-37	0.1595733810658544
1106	38-39	0.19507357594177677
1106	40-41	0.1987268262212254
1106	42-43	0.17564236979346504
1106	44-45	0.11571339289690741
1106	46-47	0.16894570947800247
1106	48-49	0.20372582611148715
1106	50-51	0.15519687351132916
1106	52-53	0.16251970044173447
1106	54-55	0.08735398847549902
1106	56-57	0.14795228930029936
1106	58-59	0.08668988649282028
1106	60-61	0.08135600586531666
1106	62-63	0.1576518637711004
1106	64-65	0.16779630572442983
1106	66-67	0.14288540640006886
1106	68-69	0.13337275857028175
1106	70-71	0.20048506184203774
1106	72-73	0.1723183490261988
1106	74-75	0.06653336303759261
1106	76-77	0.13524859258308197
1106	78-79	0.13567560226787378
1106	80-81	0.12300556189560297
1106	82-83	0.15138278757527956
1106	84-85	0.12179944441220414
1106	86-87	0.11100722216917802
1106	88-89	0.16792245867988953
1106	90-91	0.14215750581183784
1106	92-93	0.17657310334918463
1106	94-95	0.21464906515053883
1106	96-97	0.10620611501973087
1106	98-99	0.0859749459959005
1106	100	0.2676899527675367
1107	1	0.09349411130394714
1107	2	0.15528903316831588
1107	3	0.17745129281340155
1107	4	0.1459957379838812
1107	5	0.015102884146763529
1107	6	0.016670958136792535
1107	7	0.144810463195725
1107	8	0.11526031840057982
1107	9	0.10006134574462777
1107	10-11	0.15425409971570048
1107	12-13	0.09411582672638019
1107	14-15	0.20180887217886578
1107	16-17	0.18740363518314496
1107	18-19	0.16432341947427176
1107	20-21	0.17038815471545377
1107	22-23	0.1581887984088226
1107	24-25	0.14596446088557258
1107	26-27	0.15873770716952862
1107	28-29	0.180856987496405
1107	30-31	0.1609198857607197
1107	32-33	0.27036459279543124
1107	34-35	0.1863790683646016
1107	36-37	0.13377842926188066
1107	38-39	0.1645902791188263
1107	40-41	0.1682030818271727
1107	42-43	0.1951955735508335
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2216	62-63	-0.8480378633665424
2216	64-65	-0.7780613912172711
2216	66-67	-0.8389553756795536
2216	68-69	-0.8044125511137352
2216	70-71	-0.7912861271275418
2216	72-73	-0.7888758700568985
2216	74-75	-0.650217618365005
2216	76-77	-0.7715616744103428
2216	78-79	-0.7626275825359059
2216	80-81	-0.7838038247582162
2216	82-83	-0.7281466938748409
2216	84-85	-0.7504486894187394
2216	86-87	-0.7690893656747306
2216	88-89	-0.7210842979014771
2216	90-91	-0.7012680156795454
2216	92-93	-0.7012155777840299
2216	94-95	-0.7113243925933972
2216	96-97	-0.6741392920120823
2216	98-99	-0.6880037443525353
2216	100	-0.16649698132919966
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	18.0
13	71.0
14	283.0
15	826.0
16	2020.0
17	4134.0
18	7452.0
19	11375.0
20	16675.0
21	23875.0
22	33132.0
23	39406.0
24	39964.0
25	38369.0
26	38721.0
27	45092.0
28	56092.0
29	70858.0
30	88169.0
31	110521.0
32	140504.0
33	179931.0
34	254614.0
35	374590.0
36	600129.0
37	1142146.0
38	1107778.0
39	407008.0
40	1193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.40010159356287	21.516015050407947	14.03889842132869	25.044984934700487
2	14.105767243888167	20.848376814034346	39.9895585919571	25.056297350120392
3	17.24461794036425	26.688765472749264	29.936491613567206	26.13012497331928
4	11.76919806120045	17.640517217465757	38.441727331782396	32.1485573895514
5	12.582333949913657	38.507768064035005	35.12861418763526	13.781283798416085
6	30.002792169515715	38.86840608486127	17.401193393933752	13.727608351689263
7	26.007740722808038	32.596972584405755	23.53944167766816	17.85584501511805
8	26.152689830219618	34.98611775256507	19.62050502259381	19.240687394621496
9	26.006111332646114	14.661676062140844	21.256529537418363	38.07568306779467
10-11	25.329651669758046	26.084292046746278	27.55946698126476	21.02658930223092
12-13	25.905624239067365	23.870903425141808	28.01556834693968	22.20790398885115
14-15	23.049486403890214	24.822476085634275	26.111319509536635	26.016718000938877
16-17	21.636016241733113	27.297871829958186	27.26535858060225	23.80075334770645
18-19	21.146560062312957	26.90969161104992	30.083654441478654	21.86009388515847
20-21	22.551992243149467	26.172731394231008	29.522950796615977	21.75232556600355
22-23	22.81064010563083	25.78633665856455	29.322690276966558	22.08033295883806
24-25	22.296798396343956	25.963990955856435	29.888724851870297	21.85048579592931
26-27	22.26296145235775	26.20832637014804	29.10644200811094	22.422270169383275
28-29	21.567866651002948	26.590801728745188	29.55403693769866	22.287294682553206
30-31	22.321741767033053	26.132886117587102	29.504160537684758	22.041211577695083
32-33	22.6863058826644	26.458618410275843	28.760368251846142	22.09470745521362
34-35	22.60623269889753	26.359661895299062	29.316495799751475	21.717609606051933
36-37	22.406468564734457	25.899409135809414	29.117755460654003	22.576366838802123
38-39	22.50798973805738	25.96178824526076	29.635185623352623	21.89503639332924
40-41	22.15421166538985	25.4657026584752	29.981993616482644	22.3980920596523
42-43	22.335619877922262	25.590553949656584	29.22478858114827	22.849037591272882
44-45	22.541537123843213	26.451555258459646	28.830503384521705	22.17640423317543
46-47	22.607473662613405	26.328038003272837	28.107404168645317	22.957084165468448
48-49	23.328804505194675	26.155699167230615	27.953173061490578	22.56232326608413
50-51	22.779791815927027	26.30000256465835	27.701805519475688	23.218400099938943
52-53	22.27771857921237	26.815261040854182	27.50029369474609	23.40672668518736
54-55	22.382972985047214	26.759055725889315	27.381419712129514	23.47655157693396
56-57	23.248038450019774	26.313322241875408	27.201903969263814	23.236735338841005
58-59	22.346281824514495	26.57911598708736	27.893410317206875	23.181191871191274
60-61	22.51437035982984	27.067000871983836	27.29814070542996	23.120488062756362
62-63	22.82352544554734	26.46289973509561	27.00871694279488	23.704857876562166
64-65	22.11644838446073	26.565916207182326	27.405376975020836	23.912258433336113
66-67	22.68982142611535	26.28338830292917	27.345623647670898	23.681166623284586
68-69	22.330097589386614	26.161800572167003	27.64010894006807	23.86799289837831
70-71	22.329795981344493	25.904763165430367	27.643600713185755	24.121840140039385
72-73	22.77554920593555	25.996699024909333	27.862753067000902	23.364998702154217
74-75	22.921066935377787	26.411250458124336	27.40368427105076	23.263998335447116
76-77	22.606050596857937	26.38998234622962	27.043816118261542	23.960150938650898
78-79	22.420679917885423	26.450978877450993	27.214533589426903	23.913807615236678
80-81	22.14358074289052	26.859160132303277	27.55477200187303	23.442487122933176
82-83	22.784269626668475	25.98861374425227	28.39448044158453	22.832636187494725
84-85	23.016009259243937	26.516819608550406	27.127570242682864	23.339600889522792
86-87	21.905450147178296	26.971994758169267	27.544006641637807	23.57854845301463
88-89	22.62596402197995	26.055853294924127	27.411709568161175	23.90647311493475
90-91	22.301576106650074	26.12925629871817	27.54935312698037	24.019814467651386
92-93	22.570461919787416	26.35143016931709	27.325110983521654	23.75299692737384
94-95	21.608218654497502	26.30026109876582	27.892169353491	24.199350893245683
96-97	21.972234677821	26.191594042712318	27.45126528660471	24.384905992861977
98-99	21.399674702044614	26.863875794423603	27.337086283379858	24.399363220151926
100	22.04130087041898	26.283768135798358	26.626703734893603	25.04822725888906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	745.0
1	503.5
2	392.5
3	509.0
4	352.0
5	274.5
6	566.0
7	1348.0
8	2270.0
9	2498.5
10	1881.0
11	1271.5
12	1152.5
13	1257.5
14	1510.5
15	1996.0
16	2531.0
17	3146.5
18	4209.5
19	5555.5
20	7000.5
21	8621.5
22	10642.0
23	12881.5
24	14904.5
25	16984.0
26	20098.0
27	23488.5
28	27911.5
29	36567.0
30	47992.5
31	58247.5
32	72235.0
33	85952.5
34	95002.0
35	105751.0
36	116004.0
37	135041.5
38	145339.5
39	139283.5
40	132819.5
41	126327.0
42	126474.5
43	133908.5
44	145576.0
45	170014.5
46	192943.5
47	209423.5
48	221351.5
49	214845.5
50	203598.5
51	206002.0
52	205836.0
53	197847.5
54	193090.0
55	171035.0
56	148561.5
57	125088.5
58	101436.0
59	97303.5
60	81650.5
61	58234.0
62	44720.0
63	36532.0
64	26795.0
65	14831.0
66	9289.0
67	6953.0
68	5170.5
69	3716.5
70	2830.5
71	2105.5
72	1446.0
73	1133.5
74	892.5
75	698.5
76	487.5
77	226.5
78	109.5
79	53.0
80	21.0
81	13.0
82	7.0
83	3.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007342368652263767
3	0.0
4	0.08963894574341172
5	0.004570883020141668
6	4.13654571958522E-5
7	6.204818579377831E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.7921683607200237E-4
14-15	0.009565761976540821
16-17	0.0
18-19	5.170682149481526E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	6.204818579377831E-5
66-67	1.3443773588651966E-4
68-69	0.0
70-71	5.480923078450417E-4
72-73	3.5160638616474376E-4
74-75	4.1365457195852206E-4
76-77	1.5512046448444574E-4
78-79	1.034136429896305E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06496445052608589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4834952.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.42135000199187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92647162058041	46.25095281059158
2	16.13246146984633	20.462849705455447
3	5.575180504716895	10.607564223417956
4	2.356218863566807	5.97738324910264
5	1.1674488037362727	3.7020589595582436
6	0.6320788005266689	2.4052374502224647
7	0.3603414578776629	1.5997339204201089
8	0.23322508268542522	1.1833159678588678
9	0.15631539714543136	0.8922360161754687
>10	0.4455763177630842	4.143077355063332
>50	0.008421472316760518	0.35868773581939695
>100	0.0049726867485414255	0.6371718990983299
>500	6.897441947042073E-4	0.32341772788683765
>1k	4.5982945761858157E-4	0.450247633775895
>5k	4.5982945761858105E-5	0.23788263156357103
>10k+	9.196589152371621E-5	0.7681827139899503
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14228	0.29427386249129256	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12113	0.25052989150667887	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8157	0.1687090171732832	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0341364298963051E-4
2	0.0	0.0	0.0	0.0	1.0341364298963051E-4
3	0.0	0.0	0.0	0.0	1.0341364298963051E-4
4	0.0	0.0	0.0	2.06827285979261E-5	1.2409637158755662E-4
5	0.0	0.0	0.0	2.06827285979261E-5	1.654618287834088E-4
6	0.0	0.0	0.0	2.06827285979261E-5	1.8614455738133492E-4
7	0.0	0.0	0.0	2.06827285979261E-5	1.8614455738133492E-4
8	0.0	0.0	0.0	2.06827285979261E-5	2.0682728597926103E-4
9	0.0	0.0	0.0	4.13654571958522E-5	2.0682728597926103E-4
10-11	0.0	0.0	0.0	6.204818579377831E-5	2.0682728597926103E-4
12-13	0.0	0.0	0.0	6.204818579377831E-5	5.377509435460787E-4
14-15	0.0	0.0	0.0	6.204818579377831E-5	6.204818579377831E-4
16-17	0.0	0.0	0.0	7.238955009274137E-5	6.825300437315613E-4
18-19	0.0	0.0	0.0	8.27309143917044E-5	7.445782295253397E-4
20-21	0.0	0.0	0.0	1.0341364298963051E-4	8.479918725149702E-4
22-23	0.0	0.0	0.0	2.275100145771871E-4	8.996986940097854E-4
24-25	0.0	0.0	0.0	3.7228911476266984E-4	9.100400583087484E-4
26-27	0.0	0.0	0.0	7.445782295253396E-4	9.410641512056376E-4
28-29	0.0	0.0	0.0	0.002450903338854243	9.720882441025268E-4
30-31	0.0	0.0	0.0	0.009059035125891632	9.8242960840149E-4
32-33	0.0	0.0	0.0	0.02190300958520374	0.001013453701298379
34-35	0.0	0.0	0.0	0.04144818811024391	0.0010341364298963051
36-37	0.0	0.0	0.0	0.07511967026766761	0.0010341364298963051
38-39	0.0	0.0	0.0	0.12347588972961883	0.0010444777941952682
40-41	0.0	0.0	0.0	0.17656845404049515	0.0010755018870921573
42-43	0.0	0.0	0.0	0.23137768482499932	0.0010961846156900834
44-45	0.0	0.0	0.0	0.3010060906499175	0.0011065259799890464
46-47	0.0	0.0	0.0	0.38930065903446404	0.0011272087085869725
48-49	0.0	0.0	0.0	0.4906667118928999	0.0011685741657828247
50-51	0.0	0.0	0.0	0.5982996315165072	0.0013236946302672705
52-53	0.0	0.0	0.0	0.7021372704423953	0.0014891564590506794
54-55	0.0	0.0	0.0	0.8192532211281518	0.0015098391876486054
56-57	0.0	0.0	0.0	0.9665142487453857	0.0015305219162465315
58-59	0.0	0.0	0.0	1.1451199515527766	0.0015512046448444576
60-61	0.0	0.0	0.0	1.3328363963075538	0.0015718873734423837
62-63	0.0	0.0	0.0	1.5299841653029853	0.0016753010164320143
64-65	0.0	0.0	0.0	1.7373388608614935	0.0016856423807309773
66-67	0.0	0.0	0.0	1.9752109224662417	0.0016959837450299404
68-69	0.0	0.0	0.0	2.2492777591173603	0.0016959837450299404
70-71	0.0	0.0	0.0	2.5509767211753083	0.0016959837450299404
72-73	1.034136429896305E-5	0.0	0.0	2.867743051016846	0.0017373492022257925
74-75	2.06827285979261E-5	0.0	0.0	3.1812208270113125	0.0017890560237206077
76-77	2.06827285979261E-5	0.0	0.0	3.5431996015679164	0.001840762845215423
78-79	2.06827285979261E-5	0.0	0.0	3.9435862031308684	0.001851104209514386
80-81	2.06827285979261E-5	0.0	0.0	4.3790920778531	0.0019131523953081642
82-83	2.06827285979261E-5	0.0	0.0	4.838724355484811	0.0020786142240915733
84-85	2.06827285979261E-5	0.0	0.0	5.290569585799403	0.0020889555883905364
86-87	3.102409289688915E-5	0.0	0.0	5.770367523814094	0.0021820278670812037
88	4.13654571958522E-5	0.0	0.0	6.159564769205568	0.002275100145771871
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10045	0.0	47.862415	1
GTATCAA	17875	0.0	41.608253	1
GGGCACC	4335	0.0	35.04138	94
GTGGTAT	2730	0.0	34.11899	1
CAACGCA	21835	0.0	32.652893	5
ATCAACG	21995	0.0	32.464596	3
TATCAAC	23890	0.0	31.033712	2
AACGCAG	24660	0.0	29.63556	6
GTACTAG	4360	0.0	29.239939	1
TCAACGC	25465	0.0	28.243986	4
CTGTGCG	6040	0.0	27.237661	9
ACGCAGA	26075	0.0	27.198235	7
TAGGCAT	5485	0.0	26.991262	5
CGCAGAG	26400	0.0	26.845884	8
TAGTACT	6105	0.0	26.659538	4
CTAACGC	1895	0.0	25.815844	3
TAACGCC	1980	0.0	25.182734	4
CTAGTAC	6925	0.0	25.065062	3
TCTAACG	2005	0.0	24.870537	2
CAGTACG	800	0.0	24.677319	9
>>END_MODULE