FastQCFastQC Report
Mon 16 Jan 2017
ERR1146681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4892049
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC52550.038.1084194
GTACTAG50850.036.4288181
CAACGCA90800.034.2112545
GTATCAA100600.034.116521
GGTATCA63300.033.4975241
ATCAACG95250.032.5155833
CTGTGCG67850.031.5135389
TAGGCAT65850.030.9019225
TAGTACT70250.030.8404924
GCATCGT57400.030.0462158
ACTGTGC78100.028.641228
CTAGGCA73350.028.6400724
AACGCAG116050.028.629836
CTAGTAC78850.028.0730953
TATCAAC121650.028.0478842
GTACTGT77100.028.038066
TACTGTG85750.027.2917167
ACGCAGA112500.027.2352097
CGCAGAG112750.027.174828
TATTCTC25950.026.621555
Produced by FastQC (version 0.11.5)