##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146681_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4892049
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31408291290623	31.0	31.0	34.0	28.0	34.0
2	31.378876417631957	31.0	31.0	34.0	28.0	34.0
3	31.491237311809428	33.0	31.0	34.0	28.0	34.0
4	34.91083776961351	37.0	35.0	37.0	32.0	37.0
5	35.05874307473208	37.0	35.0	37.0	32.0	37.0
6	35.176613521246416	37.0	35.0	37.0	32.0	37.0
7	35.08160793156405	37.0	35.0	37.0	32.0	37.0
8	35.06870168307799	37.0	35.0	37.0	32.0	37.0
9	36.54343364099583	39.0	37.0	39.0	32.0	39.0
10-11	36.37755713403524	39.0	37.0	39.0	31.5	39.0
12-13	36.30695338497223	39.0	37.0	39.0	31.0	39.0
14-15	37.47108972130083	40.0	37.0	41.0	31.0	41.0
16-17	37.500640222532525	40.0	37.0	41.0	31.0	41.0
18-19	37.47298800563935	40.0	37.0	41.0	31.0	41.0
20-21	37.564818545357994	40.0	37.0	41.0	31.0	41.0
22-23	37.506381068546126	40.0	37.0	41.0	31.0	41.0
24-25	37.47406689916638	40.0	37.0	41.0	31.0	41.0
26-27	37.52350743011773	40.0	37.0	41.0	31.0	41.0
28-29	37.52525639052266	40.0	37.0	41.0	31.0	41.0
30-31	37.39809351868716	40.0	37.0	41.0	31.0	41.0
32-33	37.3997031714114	40.0	36.0	41.0	30.5	41.0
34-35	37.33438555092151	40.0	36.0	41.0	30.0	41.0
36-37	37.20788160543772	40.0	36.0	41.0	30.0	41.0
38-39	37.10252943091943	40.0	36.0	41.0	30.0	41.0
40-41	37.01832453027352	40.0	36.0	41.0	30.0	41.0
42-43	36.844855703612126	39.5	35.0	41.0	30.0	41.0
44-45	36.67818310895905	39.0	35.0	41.0	30.0	41.0
46-47	36.67059426428476	39.0	35.0	41.0	30.0	41.0
48-49	36.534629661313694	39.0	35.0	41.0	29.0	41.0
50-51	36.35560120105093	39.0	35.0	41.0	29.0	41.0
52-53	36.14295809383757	38.5	35.0	40.5	29.0	41.0
54-55	35.93181609587312	38.0	34.5	40.0	28.0	41.0
56-57	35.690019253691034	38.0	34.0	40.0	28.0	41.0
58-59	35.3998175406665	37.0	34.0	40.0	28.0	41.0
60-61	35.15229732981007	37.0	34.0	40.0	27.5	41.0
62-63	34.84481747832044	36.0	33.0	40.0	27.0	41.0
64-65	34.69507245328082	36.0	33.0	39.0	27.0	41.0
66-67	34.44418708806882	35.0	33.0	39.0	26.5	41.0
68-69	34.31766055491268	35.0	33.0	39.0	27.0	41.0
70-71	34.226839817017364	35.0	33.0	38.5	27.0	41.0
72-73	34.007356120104276	35.0	33.0	37.5	26.5	40.0
74-75	33.75183087904475	35.0	33.0	37.0	26.0	40.0
76-77	33.49289908993144	35.0	33.0	36.5	26.0	39.0
78-79	33.239946186148174	35.0	33.0	36.0	26.0	39.0
80-81	33.052328482400725	35.0	33.0	36.0	26.0	38.5
82-83	32.79564176483105	35.0	33.0	35.5	26.0	37.0
84-85	32.610763199632714	35.0	33.0	35.0	25.5	37.0
86-87	32.43520986809413	35.0	32.0	35.0	25.0	36.5
88-89	32.30774334026499	35.0	32.0	35.0	25.0	36.0
90-91	32.157769883335185	35.0	32.0	35.0	25.0	36.0
92-93	32.008302042763674	35.0	32.0	35.0	25.0	36.0
94-95	31.934868600048773	35.0	32.0	35.0	25.0	35.5
96-97	31.82553844002789	35.0	32.0	35.0	24.5	35.0
98-99	31.732913856750002	35.0	32.0	35.0	24.0	35.0
100	30.785175291580277	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3306808402855026
1101	2	0.429627409949223
1101	3	0.4438437499101404
1101	4	0.5610830144104924
1101	5	0.41497761693392476
1101	6	0.4278604408655582
1101	7	0.41437002259963407
1101	8	0.43534420352522574
1101	9	0.5654227570294026
1101	10-11	0.6277532104599075
1101	12-13	0.4922431372329257
1101	14-15	0.7275677242032472
1101	16-17	0.6645158708417398
1101	18-19	0.7021188283920239
1101	20-21	0.6497256735073407
1101	22-23	0.655952432107334
1101	24-25	0.7181883022691409
1101	26-27	0.6306718803171307
1101	28-29	0.6277884778991094
1101	30-31	0.6793432919317155
1101	32-33	0.7114400463806874
1101	34-35	0.7054053661083657
1101	36-37	0.6453138024487828
1101	38-39	0.6536387185340615
1101	40-41	0.7627684483977717
1101	42-43	0.6644850104582645
1101	44-45	0.6899732134382006
1101	46-47	0.715809253451944
1101	48-49	0.7350805634798476
1101	50-51	0.6065497724612285
1101	52-53	0.5782537593137747
1101	54-55	0.5621355545955637
1101	56-57	0.5633167189109969
1101	58-59	0.5777260416722712
1101	60-61	0.6101518622054982
1101	62-63	0.5692915207199931
1101	64-65	0.5747139633073672
1101	66-67	0.6544337986913007
1101	68-69	0.6351140545714884
1101	70-71	0.6325867294534149
1101	72-73	0.6153908301443707
1101	74-75	0.5766141808805685
1101	76-77	0.5631996124291518
1101	78-79	0.5376462948595417
1101	80-81	0.49838685986422604
1101	82-83	0.5114477360530998
1101	84-85	0.4981588377145343
1101	86-87	0.4603292778420709
1101	88-89	0.4742741187851536
1101	90-91	0.4755345033407963
1101	92-93	0.4297622102508214
1101	94-95	0.45236499173885747
1101	96-97	0.5008422095770868
1101	98-99	0.4201317968044087
1101	100	0.5118418187061522
1102	1	0.3718929419492696
1102	2	0.49513337809909075
1102	3	0.4483282925384451
1102	4	0.5593559925788156
1102	5	0.4988658415547249
1102	6	0.5176990714643068
1102	7	0.4668445321107697
1102	8	0.5095596021691549
1102	9	0.6553744673336297
1102	10-11	0.7673947485453922
1102	12-13	0.6992567781490706
1102	14-15	0.8664625715806054
1102	16-17	0.77979215335845
1102	18-19	0.8703410740722646
1102	20-21	0.7956512365356261
1102	22-23	0.8048258680229026
1102	24-25	0.8503221448821918
1102	26-27	0.7673639666098779
1102	28-29	0.7807006600043493
1102	30-31	0.8404931313382349
1102	32-33	0.7529464071517182
1102	34-35	0.8204747932130516
1102	36-37	0.8747157401266819
1102	38-39	0.8476425919650907
1102	40-41	0.7890833702648834
1102	42-43	0.7959749979796484
1102	44-45	0.7731435059860772
1102	46-47	0.7639972977843001
1102	48-49	0.7206633145542014
1102	50-51	0.7339882321772961
1102	52-53	0.7027253052945497
1102	54-55	0.6525096972968853
1102	56-57	0.6502663660369024
1102	58-59	0.6750502630669928
1102	60-61	0.712916634689357
1102	62-63	0.691400347455648
1102	64-65	0.6745286637141845
1102	66-67	0.6339671426559335
1102	68-69	0.5901229554630731
1102	70-71	0.6087752185859969
1102	72-73	0.6625072863767798
1102	74-75	0.5791577035975948
1102	76-77	0.6245756901918966
1102	78-79	0.6409327640231766
1102	80-81	0.6327744584490205
1102	82-83	0.6375018904052467
1102	84-85	0.677903803881982
1102	86-87	0.7210371327388572
1102	88-89	0.6186354940057939
1102	90-91	0.6563967706806153
1102	92-93	0.5851732921972896
1102	94-95	0.5747073649074501
1102	96-97	0.6626306786500535
1102	98-99	0.6275173075498692
1102	100	0.7003027941404127
1103	1	0.2854047950094589
1103	2	0.34372594599897965
1103	3	0.3587855429007121
1103	4	0.45911986366685653
1103	5	0.3033062003844549
1103	6	0.39720615411371085
1103	7	0.4016189488729438
1103	8	0.34206847610315094
1103	9	0.43817975173761425
1103	10-11	0.5899147214019038
1103	12-13	0.46058838606597874
1103	14-15	0.6368392920114019
1103	16-17	0.6376829476673436
1103	18-19	0.668109696821908
1103	20-21	0.5826844235392556
1103	22-23	0.6797141387958732
1103	24-25	0.6586541390764467
1103	26-27	0.6603357636395515
1103	28-29	0.6366731514179307
1103	30-31	0.5854996127221881
1103	32-33	0.5490401156880722
1103	34-35	0.6007789078233685
1103	36-37	0.5816229887579709
1103	38-39	0.6606985853500262
1103	40-41	0.6255367684831654
1103	42-43	0.5672010229303766
1103	44-45	0.5241295171067009
1103	46-47	0.5202683993745012
1103	48-49	0.5701929168848849
1103	50-51	0.5064796236105877
1103	52-53	0.5113563082699812
1103	54-55	0.5150796477835584
1103	56-57	0.5555392843042881
1103	58-59	0.5785290363777023
1103	60-61	0.6007337735191172
1103	62-63	0.501886282583051
1103	64-65	0.4289751529344059
1103	66-67	0.5205251708802336
1103	68-69	0.4680229789438286
1103	70-71	0.46290410855127817
1103	72-73	0.49887188435712915
1103	74-75	0.47366744988505616
1103	76-77	0.39186280084843617
1103	78-79	0.41194058988553195
1103	80-81	0.36136450332968195
1103	82-83	0.4343338662563099
1103	84-85	0.43213707657082523
1103	86-87	0.4346922583026114
1103	88-89	0.4401597858903301
1103	90-91	0.43392082148321265
1103	92-93	0.37571135741948325
1103	94-95	0.35592376724885
1103	96-97	0.372911801158871
1103	98-99	0.3245211889986699
1103	100	0.4848172063156859
1104	1	0.2869716492078922
1104	2	0.33066070571936024
1104	3	0.3215484539306317
1104	4	0.4333190919097305
1104	5	0.30470582771476273
1104	6	0.3269408609986115
1104	7	0.35428964146661457
1104	8	0.3545053332126713
1104	9	0.37750948490560177
1104	10-11	0.5377697546869697
1104	12-13	0.44562587745776483
1104	14-15	0.5646623034030895
1104	16-17	0.535602678770644
1104	18-19	0.48729788988686806
1104	20-21	0.46456210939640386
1104	22-23	0.5124113517241469
1104	24-25	0.5480917874357871
1104	26-27	0.4815716522695368
1104	28-29	0.47560745203387
1104	30-31	0.4729853618895774
1104	32-33	0.5008947437301288
1104	34-35	0.4753472149975835
1104	36-37	0.44791459189168137
1104	38-39	0.42860756981870196
1104	40-41	0.49779192026335295
1104	42-43	0.5591175787750444
1104	44-45	0.5195983614844053
1104	46-47	0.4684351876568229
1104	48-49	0.4379029347232759
1104	50-51	0.3681676788159791
1104	52-53	0.40785909944543164
1104	54-55	0.4332293414788282
1104	56-57	0.41109464037312193
1104	58-59	0.38318881818766926
1104	60-61	0.4318272628282642
1104	62-63	0.48094842711374497
1104	64-65	0.42620468848229365
1104	66-67	0.3974075311387182
1104	68-69	0.4488124077435316
1104	70-71	0.4289146705181892
1104	72-73	0.3794474705168014
1104	74-75	0.3930565973229818
1104	76-77	0.39933222487755415
1104	78-79	0.3720655740156573
1104	80-81	0.34607600492179813
1104	82-83	0.44406470024901523
1104	84-85	0.40354884836770566
1104	86-87	0.4191144471535537
1104	88-89	0.3648742883326932
1104	90-91	0.4796519837740689
1104	92-93	0.488819045623444
1104	94-95	0.5112795796008136
1104	96-97	0.4564609862484943
1104	98-99	0.44807388790438907
1104	100	0.5843461754210324
1105	1	0.09230205881947384
1105	2	0.1829006099338315
1105	3	0.20903192771576684
1105	4	0.24726414367886917
1105	5	0.17518663620334252
1105	6	0.17975933182485448
1105	7	0.19609681345394137
1105	8	0.17764689670040212
1105	9	0.26610137341271667
1105	10-11	0.2821082244122337
1105	12-13	0.20051222389127332
1105	14-15	0.35240593031662115
1105	16-17	0.314241313184624
1105	18-19	0.29144744926426114
1105	20-21	0.2575775644464713
1105	22-23	0.2815213445218774
1105	24-25	0.2711015999475421
1105	26-27	0.2581685594245471
1105	28-29	0.321782636792598
1105	30-31	0.3679768980821052
1105	32-33	0.272534887408419
1105	34-35	0.30560117509612894
1105	36-37	0.3266735087078203
1105	38-39	0.285765060861749
1105	40-41	0.23732744302388653
1105	42-43	0.25828711640802027
1105	44-45	0.20595507082945375
1105	46-47	0.27201572227586013
1105	48-49	0.14099197214293469
1105	50-51	0.21453466400316756
1105	52-53	0.24598628134160094
1105	54-55	0.196152268075366
1105	56-57	0.2030794835059737
1105	58-59	0.2197996711284489
1105	60-61	0.31982374885725307
1105	62-63	0.29073125820000456
1105	64-65	0.20222247853399722
1105	66-67	0.21184986026843688
1105	68-69	0.21670370401348293
1105	70-71	0.21209591527131977
1105	72-73	0.25267312158774047
1105	74-75	0.18553110268339168
1105	76-77	0.1600275874450503
1105	78-79	0.2415468274849033
1105	80-81	0.1512676906645254
1105	82-83	0.17889686913981961
1105	84-85	0.164253248171363
1105	86-87	0.13806355879782473
1105	88-89	0.12977393831182127
1105	90-91	0.13556742827305612
1105	92-93	0.1655511902008726
1105	94-95	0.09762959129875881
1105	96-97	0.10253143999114656
1105	98-99	0.08323548010942616
1105	100	0.10915967347366973
1106	1	0.061038222559123056
1106	2	0.17085275943217937
1106	3	0.15454085195453615
1106	4	0.2888269395072456
1106	5	0.12864191159387417
1106	6	0.1571629807172883
1106	7	0.23067562456307655
1106	8	0.1332932930191575
1106	9	0.2801163306720085
1106	10-11	0.3435995927424784
1106	12-13	0.2308409265023883
1106	14-15	0.34188823342694974
1106	16-17	0.28001100391885103
1106	18-19	0.3073993990024988
1106	20-21	0.27729392276662423
1106	22-23	0.2828492366019262
1106	24-25	0.3124899150417164
1106	26-27	0.35818886247654547
1106	28-29	0.359371664835912
1106	30-31	0.3981537388140737
1106	32-33	0.3279239290637008
1106	34-35	0.3297750035163318
1106	36-37	0.33006393277378976
1106	38-39	0.371099318748513
1106	40-41	0.3302526381656534
1106	42-43	0.27817414587794076
1106	44-45	0.2783258255956369
1106	46-47	0.28729285407422367
1106	48-49	0.19888230207416058
1106	50-51	0.21934216508373083
1106	52-53	0.20044360117963578
1106	54-55	0.23035112838206118
1106	56-57	0.2757875676915944
1106	58-59	0.25742652984767034
1106	60-61	0.2380767052626993
1106	62-63	0.3406878766679071
1106	64-65	0.2956974912841801
1106	66-67	0.28832851377922
1106	68-69	0.2032950098524111
1106	70-71	0.2737743863463251
1106	72-73	0.1767350755067909
1106	74-75	0.19914012264791126
1106	76-77	0.17209433560676501
1106	78-79	0.15656603892744414
1106	80-81	0.13409908694350747
1106	82-83	0.19757783368063286
1106	84-85	0.18238645457747538
1106	86-87	0.17374225261418985
1106	88-89	0.13940654764733296
1106	90-91	0.17811497073753912
1106	92-93	0.23604670134368888
1106	94-95	0.2607725426394971
1106	96-97	0.23118821234505305
1106	98-99	0.20743184783420432
1106	100	0.16918624333623455
1107	1	0.13042710841230587
1107	2	0.11478307077164374
1107	3	0.14609979654342098
1107	4	0.15313139543330578
1107	5	0.1161526215183315
1107	6	0.14301291680989436
1107	7	0.032332187028664805
1107	8	0.11774366500084454
1107	9	0.13136808596620853
1107	10-11	0.19742343183482802
1107	12-13	0.07624377120605885
1107	14-15	0.23548553903609815
1107	16-17	0.14368201676619918
1107	18-19	0.1171388425321922
1107	20-21	0.14263920718249068
1107	22-23	0.26558129510435435
1107	24-25	0.3041943473073516
1107	26-27	0.20774908249743618
1107	28-29	0.20250516974530086
1107	30-31	0.2708594087434051
1107	32-33	0.26477168556605335
1107	34-35	0.2243018152357763
1107	36-37	0.2256720933514842
1107	38-39	0.20893093804129848
1107	40-41	0.24085638586156932
1107	42-43	0.2654777909258854
1107	44-45	0.2639891833161485
1107	46-47	0.23809101480436823
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2216	82-83	-1.0435308091033946
2216	84-85	-1.1259906705348115
2216	86-87	-1.0758278953615559
2216	88-89	-1.0783819208711165
2216	90-91	-1.1970448874036066
2216	92-93	-1.1646712309009004
2216	94-95	-1.1649182315647053
2216	96-97	-1.2093453505064744
2216	98-99	-1.1819535700718085
2216	100	-0.8671476398996703
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	10.0
3	23.0
4	49.0
5	33.0
6	56.0
7	214.0
8	569.0
9	937.0
10	1363.0
11	2452.0
12	4255.0
13	6413.0
14	8964.0
15	11893.0
16	14565.0
17	17133.0
18	19686.0
19	22244.0
20	25897.0
21	29175.0
22	33564.0
23	39058.0
24	44891.0
25	51467.0
26	57881.0
27	63864.0
28	73408.0
29	87463.0
30	103460.0
31	119170.0
32	147430.0
33	190336.0
34	277184.0
35	397663.0
36	636248.0
37	1089821.0
38	947838.0
39	364689.0
40	682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.921533130749076	20.695583841720016	12.343697556164567	24.03918547136634
2	15.559243920977305	19.78891410642363	40.75910458171851	23.892737390880555
3	18.43092769258257	26.66264211485632	29.063226622062693	25.843203570498414
4	11.967890894788015	16.574589927328066	40.35451180111424	31.103007376769675
5	12.859171288946635	38.789374895490205	35.319567953954774	13.031885861608385
6	29.854458141844336	38.79964660135906	18.044344906280266	13.301550350516342
7	26.79545503326346	32.091177339352676	23.750118816313556	17.36324881107031
8	26.13761452699312	35.21992882620474	19.944811588135575	18.697645058666563
9	26.223867352764596	15.13088783697018	21.37445260264026	37.27079220762496
10-11	25.53599991823427	25.954425825706124	27.78710247801268	20.722471778046923
12-13	25.541295532034564	24.006086217876746	27.72145097445614	22.73116727563255
14-15	23.092149528106198	25.25929092821844	25.0593131479718	26.589246395703555
16-17	22.01649726672353	27.83211851539931	26.389136599433055	23.76224761844411
18-19	21.41457692466115	28.549767716349123	27.983278316318895	22.052377042670834
20-21	23.178959295511543	27.661090185238013	26.94051629357992	22.219434225670526
22-23	23.679297283711932	27.284426363635156	26.584220302234975	22.452056050417944
24-25	23.070701764158468	27.263829615437878	27.49735409447173	22.168114525931916
26-27	22.9139659711082	27.549113350767012	26.42895946286417	23.10796121526062
28-29	21.96421847422266	28.120984395691284	27.01340841325191	22.90138871683415
30-31	22.86230661585632	27.73356476893764	26.958449016213	22.445679598993046
32-33	23.297272948517282	27.917011677930702	26.013346761388078	22.77236861216393
34-35	23.204012121321146	27.696848816282344	27.03095908399968	22.068179978396834
36-37	22.832015999556795	27.277962554650685	26.70695721298122	23.183064232811297
38-39	23.03572790200917	27.41514595164117	27.27130093016305	22.27782521618661
40-41	22.657337053485417	26.585605456386592	27.9000162621221	22.85704122800589
42-43	22.552865161739835	26.843718004494498	27.21890363975964	23.384513194006026
44-45	22.76374492616371	27.83696784905734	27.039057064340117	22.360230160438828
46-47	22.752357183324122	27.512429175118218	26.58701608406921	23.148197557488448
48-49	23.380054854603813	27.149256598362335	26.801153032363278	22.669535514670574
50-51	23.033487159927898	27.005670590142717	26.788695457588542	23.172146792340843
52-53	22.483750483820412	27.607370304948454	26.650678698059465	23.258200513171673
54-55	22.432252319353747	27.504259547217824	26.705324181010237	23.3581639524182
56-57	23.536913725883053	26.94454609947928	26.440208780132767	23.078331394504904
58-59	22.45660477732328	27.374620973517676	27.252686434210037	22.916087814949
60-61	22.478434391954426	27.97030689782564	26.566469790770718	22.984788919449212
62-63	22.865461705966514	27.2816360959535	26.2557182411394	23.597183956940583
64-65	22.03573166293908	27.514249324332614	26.675481349126844	23.77453766360146
66-67	22.79452375181737	27.044175917792696	26.599788626494387	23.561511703895547
68-69	22.234592194978752	27.137486318869186	26.88422441429224	23.74369707185982
70-71	22.140940082326786	26.849355226767663	27.075303629985548	23.934401060920003
72-73	22.781636257945117	26.896531844906168	27.49351363401778	22.828318263130935
74-75	22.934571571268904	27.13255552986456	27.016751825168196	22.91612107369834
76-77	22.622963052309	27.036303718074787	26.770761993740173	23.56997123587604
78-79	22.46372174675335	27.047096053116583	26.798365058554463	23.6908171415756
80-81	22.056905139088983	27.411622875476667	27.417111979145904	23.114360006288447
82-83	22.823297855638273	26.387264826710975	28.4582371161671	22.331200201483654
84-85	23.07612105952537	26.931228407745582	27.121476369980563	22.871174162748485
86-87	21.774844025138332	27.668867696662435	27.473736211875384	23.082552066323853
88-89	22.887617669170435	26.37024180602468	27.287605198883192	23.45453532592169
90-91	22.487944811242112	26.465643440365287	27.531134755303665	23.515276993088936
92-93	22.769354560583434	26.658946638276532	27.44592751807152	23.125771283068513
94-95	21.610492074868745	26.680321216504684	28.114713346446823	23.594473362179748
96-97	22.001560852550544	26.567908074268775	27.613311302177273	23.817219771003405
98-99	21.287988546833503	27.27762623597767	27.492485546060543	23.941899671128287
100	22.15220188974964	26.563974733652536	26.708613279732834	24.57521009686499
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1254.0
1	889.5
2	683.0
3	823.0
4	763.5
5	670.0
6	599.0
7	579.0
8	611.5
9	619.0
10	534.5
11	477.5
12	483.5
13	514.0
14	551.0
15	811.5
16	999.0
17	1144.5
18	1576.5
19	2000.0
20	2544.5
21	3230.0
22	4455.5
23	6314.0
24	8189.5
25	10293.5
26	13591.0
27	17656.5
28	22870.5
29	32823.5
30	46978.5
31	59732.0
32	76207.5
33	92449.0
34	103479.0
35	116276.0
36	128337.5
37	150003.0
38	158018.0
39	145754.0
40	135561.0
41	126116.5
42	125035.5
43	133228.0
44	146063.0
45	173687.5
46	199817.5
47	215944.0
48	226317.5
49	221450.5
50	210957.5
51	216796.0
52	220537.0
53	211189.0
54	200738.0
55	173899.0
56	152369.5
57	125400.5
58	97950.0
59	94323.5
60	78422.0
61	54400.5
62	41209.0
63	33920.5
64	24399.0
65	12339.0
66	7092.5
67	4996.0
68	3703.5
69	2635.5
70	1928.5
71	1324.5
72	944.5
73	703.5
74	437.0
75	306.5
76	200.0
77	101.5
78	64.5
79	50.5
80	41.0
81	31.0
82	26.0
83	19.5
84	19.5
85	20.5
86	19.5
87	19.5
88	14.5
89	11.5
90	14.5
91	14.0
92	10.0
93	12.0
94	13.0
95	6.0
96	4.0
97	9.0
98	7.0
99	4.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05907545079781499
2	0.007992560990292616
3	0.008319622309588479
4	0.009750515581507871
5	0.0033319371903265892
6	0.0025142838920869355
7	8.99418628063619E-4
8	0.0016353065964793078
9	7.358879684156883E-4
10-11	4.905919789437922E-4
12-13	2.555166556998918E-4
14-15	1.1242732850795238E-4
16-17	5.416953100837706E-4
18-19	0.0015842032653393292
20-21	9.300806267476061E-4
22-23	0.0012367006135874763
24-25	0.0011549352837635111
26-27	0.0036078951784824726
28-29	0.003986059828918313
30-31	0.005386291102153719
32-33	0.006142620403025398
34-35	0.0035363505148865025
36-37	0.008452490970552421
38-39	0.0066638743806531784
40-41	0.006408357724953286
42-43	0.015065262020065622
44-45	0.02097280709984712
46-47	0.0191330871788079
48-49	0.02288407168448231
50-51	0.0191228665125799
52-53	0.02632843620331685
54-55	0.01998140247573154
56-57	0.015923797983217258
58-59	0.01839719921039221
60-61	0.01659836195426497
62-63	0.011763986828423018
64-65	0.012039944816578902
66-67	0.014462242712613874
68-69	0.00562136642539762
70-71	0.009106613609144143
72-73	0.00904528961177617
74-75	0.011896855489386963
76-77	0.011017878193779335
78-79	0.012673626122714634
80-81	0.011038319526235326
82-83	0.006888729037669083
84-85	0.010997436861323344
86-87	0.009719853582823885
88-89	0.008616021630200352
90-91	0.014114740060862023
92-93	0.009065730944232162
94-95	0.008074326320116582
96-97	0.010046914902119746
98-99	0.00973007424905188
100	0.26553290860332757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4892049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.64856662682936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24435131785455	48.740352441559004
2	15.2163362917694	19.9786133373293
3	5.276313390439274	10.391472334688537
4	2.2924279511389267	6.01978436350199
5	1.1197182576775853	3.675394932121212
6	0.6053959872641724	2.38460272833163
7	0.3661038562181478	1.6823935378093364
8	0.2402499668932924	1.2617652766950282
9	0.16352097769273108	0.966142601905098
>10	0.4698870555209385	4.500889459210461
>50	0.004594160630152797	0.20062405578540077
>100	9.659046944381283E-4	0.09170705152732533
>500	4.4960739485874997E-5	0.015141749561874807
>1k	8.992146700411601E-5	0.09111612997377305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.176532982396538E-5
2	0.0	0.0	0.0	0.0	8.176532982396538E-5
3	0.0	0.0	0.0	0.0	8.176532982396538E-5
4	0.0	0.0	0.0	0.0	8.176532982396538E-5
5	0.0	0.0	0.0	0.0	8.176532982396538E-5
6	0.0	0.0	0.0	0.0	2.0441332455991345E-4
7	0.0	0.0	0.0	0.0	2.4529598947189616E-4
8	0.0	0.0	0.0	0.0	2.657373219278875E-4
9	0.0	0.0	0.0	0.0	2.657373219278875E-4
10-11	0.0	0.0	0.0	2.0441332455991345E-5	2.8617865438387884E-4
12-13	0.0	0.0	0.0	2.0441332455991345E-5	5.110333113997836E-4
14-15	0.0	0.0	0.0	2.0441332455991345E-5	6.030193074517446E-4
16-17	0.0	0.0	0.0	2.0441332455991345E-5	6.950053035037057E-4
18-19	0.0	0.0	0.0	2.0441332455991345E-5	7.563293008716797E-4
20-21	0.0	0.0	0.0	3.066199868398702E-5	9.198599605196105E-4
22-23	0.0	0.0	0.0	1.0220666227995674E-4	9.607426254315932E-4
24-25	0.0	0.0	0.0	2.1463399078790912E-4	9.607426254315932E-4
26-27	0.0	0.0	0.0	5.519159763117663E-4	0.001032287289027563
28-29	0.0	0.0	0.0	0.0025142838920869355	0.0010833906201675413
30-31	0.0	0.0	0.0	0.009484778259579984	0.0010833906201675413
32-33	0.0	0.0	0.0	0.022311714375714553	0.0011038319526235326
34-35	0.0	0.0	0.0	0.042058041528202195	0.0012060386149034892
36-37	0.0	0.0	0.0	0.07587822607663987	0.0012060386149034892
38-39	0.0	0.0	0.0	0.1263580965767105	0.0012367006135874763
40-41	0.0	0.0	0.0	0.18180521086358703	0.001246921279815472
42-43	0.0	0.0	0.0	0.24035940768377423	0.0013184659434114419
44-45	0.0	0.0	0.0	0.31158723062667604	0.001400231273235407
46-47	0.0	0.0	0.0	0.4020912300755778	0.0014922172692873681
48-49	0.0	0.0	0.0	0.5076604915445451	0.0015637619328833377
50-51	0.0	0.0	0.0	0.6202513507121453	0.0016557479289352989
52-53	0.0	0.0	0.0	0.7324538245630818	0.0017375132587592642
54-55	0.0	0.0	0.0	0.8578511785143608	0.0017988372561272383
56-57	0.0	0.0	0.0	1.010864772613684	0.001839719921039221
58-59	0.0	0.0	0.0	1.1983731152324926	0.0018601612534952124
60-61	0.0	0.0	0.0	1.3920649609192386	0.0018601612534952124
62-63	0.0	0.0	0.0	1.5972857181111637	0.0018806025859512037
64-65	0.0	0.0	0.0	1.8146997301130876	0.0019112645846351907
66-67	0.0	0.0	0.0	2.059617554934548	0.0019419265833191778
68-69	0.0	0.0	0.0	2.337987620320238	0.0019623679157751693
70-71	0.0	0.0	0.0	2.6468663743964953	0.002003250580687152
72-73	0.0	0.0	0.0	2.9733757777160448	0.0021565605741070866
74-75	0.0	0.0	0.0	3.298852893746567	0.0023200912337550178
76-77	0.0	0.0	0.0	3.6684321845508903	0.002371194564894996
78-79	0.0	0.0	0.0	4.074570798452754	0.002401856563578983
80-81	0.0	0.0	0.0	4.517892196092067	0.0024529598947189615
82-83	0.0	0.0	0.0	4.989177336531175	0.002544945890770922
84-85	0.0	0.0	0.0	5.443741467021283	0.002555166556998918
86-87	0.0	0.0	0.0	5.928589431544942	0.0026573732192788748
88	0.0	0.0	0.0	6.320705291381995	0.0027595798815588314
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5255	0.0	38.10841	94
GTACTAG	5085	0.0	36.428818	1
CAACGCA	9080	0.0	34.211254	5
GTATCAA	10060	0.0	34.11652	1
GGTATCA	6330	0.0	33.497524	1
ATCAACG	9525	0.0	32.515583	3
CTGTGCG	6785	0.0	31.513538	9
TAGGCAT	6585	0.0	30.901922	5
TAGTACT	7025	0.0	30.840492	4
GCATCGT	5740	0.0	30.046215	8
ACTGTGC	7810	0.0	28.64122	8
CTAGGCA	7335	0.0	28.640072	4
AACGCAG	11605	0.0	28.62983	6
CTAGTAC	7885	0.0	28.073095	3
TATCAAC	12165	0.0	28.047884	2
GTACTGT	7710	0.0	28.03806	6
TACTGTG	8575	0.0	27.291716	7
ACGCAGA	11250	0.0	27.235209	7
CGCAGAG	11275	0.0	27.17482	8
TATTCTC	2595	0.0	26.62155	5
>>END_MODULE