FastQCFastQC Report
Mon 16 Jan 2017
ERR1146681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4892049
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165640.3385902308010406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138490.2830920131830241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93530.19118778246088705No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC50690.10361711421942013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100400.048.727811
GTATCAA185350.041.9524731
GTGGTAT24200.041.0151561
GGGCACC50350.040.6262994
CAACGCA229000.032.981235
ATCAACG229400.032.8911743
CTGTGCG69050.032.337089
TATCAAC246850.031.5195262
TAGTACT72150.031.0992874
GTACTAG51950.031.0588841
AACGCAG260300.030.061376
ACTGTGC76800.029.9246928
GTACTGT78250.028.7686776
TCAACGC271300.028.1235074
TACTGTG85150.028.0383367
ACGCAGA273300.027.5999667
TAGGCAT67200.027.4858885
CGCAGAG275650.027.3481798
CTAGTAC84300.026.951813
GCAGAGT292350.025.6304389
Produced by FastQC (version 0.11.5)