##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4892049
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.132063272465174	33.0	31.0	34.0	30.0	34.0
2	32.19264831566487	33.0	31.0	34.0	30.0	34.0
3	32.34228908990895	34.0	31.0	34.0	30.0	34.0
4	35.9522972889274	37.0	35.0	37.0	35.0	37.0
5	35.86377528107343	37.0	35.0	37.0	35.0	37.0
6	35.908317353321685	37.0	35.0	37.0	35.0	37.0
7	35.83366172333924	37.0	35.0	37.0	35.0	37.0
8	35.84284846697161	37.0	35.0	37.0	35.0	37.0
9	37.54608794801524	39.0	37.0	39.0	35.0	39.0
10-11	37.48563291168997	39.0	37.0	39.0	34.5	39.0
12-13	37.454700167557604	39.0	37.0	39.0	34.0	39.0
14-15	38.662519835757976	40.0	38.0	41.0	34.0	41.0
16-17	38.66554218896826	40.0	38.0	41.0	34.0	41.0
18-19	38.73603105774288	40.0	38.0	41.0	34.0	41.0
20-21	38.73840153686114	40.0	38.0	41.0	34.0	41.0
22-23	38.70108016088964	40.0	38.0	41.0	34.0	41.0
24-25	38.62368099747161	40.0	38.0	41.0	34.0	41.0
26-27	38.477685526044404	40.0	38.0	41.0	34.0	41.0
28-29	38.41581502965322	40.0	38.0	41.0	34.0	41.0
30-31	38.26904350303932	40.0	37.5	41.0	34.0	41.0
32-33	38.10791541540161	40.0	37.0	41.0	33.0	41.0
34-35	37.99786991095142	40.0	37.0	41.0	33.0	41.0
36-37	37.97630859789017	40.0	37.0	41.0	33.0	41.0
38-39	37.8723943689035	40.0	37.0	41.0	33.0	41.0
40-41	37.749291247900416	40.0	37.0	41.0	33.0	41.0
42-43	37.60335382985738	40.0	36.0	41.0	32.5	41.0
44-45	37.38219189954965	40.0	36.0	41.0	32.0	41.0
46-47	37.10459788935066	40.0	36.0	41.0	31.0	41.0
48-49	36.803814924993596	39.0	35.0	41.0	30.5	41.0
50-51	36.58275223735494	39.0	35.0	41.0	30.0	41.0
52-53	36.39691068098459	39.0	35.0	41.0	30.0	41.0
54-55	36.210063819884056	39.0	35.0	41.0	30.0	41.0
56-57	35.9873260672573	38.0	35.0	40.5	29.5	41.0
58-59	35.713028017503504	37.5	35.0	40.0	29.0	41.0
60-61	35.53252328421077	37.0	35.0	40.0	29.0	41.0
62-63	35.32249462341853	37.0	34.5	40.0	29.0	41.0
64-65	35.075277046489106	36.0	34.0	39.5	29.0	41.0
66-67	34.82719919608328	36.0	34.0	39.0	29.0	41.0
68-69	34.57297330832132	35.0	34.0	39.0	28.0	41.0
70-71	34.352942499145044	35.0	34.0	38.5	28.0	41.0
72-73	34.114981984031644	35.0	34.0	37.5	28.0	40.0
74-75	33.860909099643116	35.0	34.0	37.0	28.0	39.5
76-77	33.620626857989365	35.0	33.5	36.5	27.5	39.0
78-79	33.39543021748147	35.0	33.0	36.0	27.0	39.0
80-81	33.26422752511269	35.0	33.0	36.0	27.0	38.5
82-83	33.10214523607593	35.0	33.0	35.5	27.0	37.0
84-85	32.96805479667109	35.0	33.0	35.0	27.0	37.0
86-87	32.823128202518006	35.0	33.0	35.0	27.0	36.5
88-89	32.71828266642464	35.0	33.0	35.0	27.0	36.0
90-91	32.651312568619	35.0	33.0	35.0	27.0	36.0
92-93	32.58293743582699	35.0	33.0	35.0	27.0	36.0
94-95	32.532616598893426	35.0	33.0	35.0	27.0	35.5
96-97	32.44216462263563	35.0	33.0	35.0	27.0	35.0
98-99	32.36666497003607	35.0	33.0	35.0	27.0	35.0
100	31.154469630210162	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31868680588873843
1101	2	0.3981849005478182
1101	3	0.34431714841969097
1101	4	0.27538690727932646
1101	5	0.24672557945363138
1101	6	0.285153329022684
1101	7	0.3043369859245928
1101	8	0.2946179812304024
1101	9	0.3564487150885469
1101	10-11	0.4007548569043138
1101	12-13	0.3694462142168575
1101	14-15	0.5071652732320189
1101	16-17	0.5560673015689446
1101	18-19	0.5193000296107826
1101	20-21	0.5181391327293454
1101	22-23	0.4756079429221671
1101	24-25	0.42271772089804216
1101	26-27	0.49382063703988877
1101	28-29	0.4827331736697076
1101	30-31	0.5526092352131897
1101	32-33	0.5435459633765234
1101	34-35	0.525023403036947
1101	36-37	0.46182326625594783
1101	38-39	0.40514466513847225
1101	40-41	0.4513458256806189
1101	42-43	0.4363931307516822
1101	44-45	0.41720316556635595
1101	46-47	0.5002193107205883
1101	48-49	0.4556077436775894
1101	50-51	0.44974131067770884
1101	52-53	0.49597623188637385
1101	54-55	0.44937244183167024
1101	56-57	0.4481215459917536
1101	58-59	0.38780045613781766
1101	60-61	0.4051192554152152
1101	62-63	0.3654924926044316
1101	64-65	0.4124811014499059
1101	66-67	0.36680095280063796
1101	68-69	0.4121463867873203
1101	70-71	0.3928157775781571
1101	72-73	0.3933353587688444
1101	74-75	0.3629630549641263
1101	76-77	0.3825480626886062
1101	78-79	0.35927209924269476
1101	80-81	0.31995152081621114
1101	82-83	0.33070538525470994
1101	84-85	0.2768700552545269
1101	86-87	0.3171855652398108
1101	88-89	0.3504363644470061
1101	90-91	0.3544006230386145
1101	92-93	0.3352329218509382
1101	94-95	0.35063242731840916
1101	96-97	0.3046457684186734
1101	98-99	0.3746933380465691
1101	100	0.7815406586686251
1102	1	0.37435423817417757
1102	2	0.41583518396510755
1102	3	0.3310205464422822
1102	4	0.21382996965519396
1102	5	0.25587379529421383
1102	6	0.2548416899255841
1102	7	0.30420623751513887
1102	8	0.27755696788737083
1102	9	0.3665429208588762
1102	10-11	0.33552903169500325
1102	12-13	0.42493686548002074
1102	14-15	0.5131603020857654
1102	16-17	0.45569252204241195
1102	18-19	0.42500800217361956
1102	20-21	0.4782930474412339
1102	22-23	0.5137443367457024
1102	24-25	0.5383917748122542
1102	26-27	0.5527848803197415
1102	28-29	0.5835214008693939
1102	30-31	0.6300710326557066
1102	32-33	0.6221637075822599
1102	34-35	0.6237455008639685
1102	36-37	0.6320701950404697
1102	38-39	0.6713931436562959
1102	40-41	0.6939846488757624
1102	42-43	0.6128811532876313
1102	44-45	0.6445328274181961
1102	46-47	0.6942264547415036
1102	48-49	0.6677105916245551
1102	50-51	0.6877383961685979
1102	52-53	0.6676280669917958
1102	54-55	0.695612804775422
1102	56-57	0.6734485166368174
1102	58-59	0.6284576777920137
1102	60-61	0.5661313729131479
1102	62-63	0.6947221942522006
1102	64-65	0.6612886894891119
1102	66-67	0.6363029915811111
1102	68-69	0.6109617302597812
1102	70-71	0.6330719760414638
1102	72-73	0.648060327346748
1102	74-75	0.5722164484642747
1102	76-77	0.5472365299796635
1102	78-79	0.5898013002345834
1102	80-81	0.520496198089063
1102	82-83	0.528245800159489
1102	84-85	0.4995612875849744
1102	86-87	0.5052042215867516
1102	88-89	0.4834908405199343
1102	90-91	0.49568256871329197
1102	92-93	0.47591033469855404
1102	94-95	0.4214058684443742
1102	96-97	0.4109520070575954
1102	98-99	0.48786849211954575
1102	100	0.8713975218661609
1103	1	0.28650094443458585
1103	2	0.374984048078673
1103	3	0.2740363708218396
1103	4	0.24539243826046686
1103	5	0.26980277326252633
1103	6	0.27786796807878744
1103	7	0.2674524612351945
1103	8	0.2404309392384789
1103	9	0.3012495379381477
1103	10-11	0.34945140316183654
1103	12-13	0.3576356597233783
1103	14-15	0.46334139404228836
1103	16-17	0.46091298690242866
1103	18-19	0.3996810929186765
1103	20-21	0.39828451287472433
1103	22-23	0.38811312981759727
1103	24-25	0.4497224674149862
1103	26-27	0.4709401812325993
1103	28-29	0.5412176650810281
1103	30-31	0.46105421073133357
1103	32-33	0.4981631070180583
1103	34-35	0.5138143125595604
1103	36-37	0.4929448766458506
1103	38-39	0.4729056597287311
1103	40-41	0.4963281816385816
1103	42-43	0.5269494103323566
1103	44-45	0.5812634698705637
1103	46-47	0.5451476483318558
1103	48-49	0.503007150833092
1103	50-51	0.46159818229067895
1103	52-53	0.4579317121345312
1103	54-55	0.42071293756240635
1103	56-57	0.3851019829721878
1103	58-59	0.4042488506349926
1103	60-61	0.4381936665384032
1103	62-63	0.4550927407412644
1103	64-65	0.41888367492565237
1103	66-67	0.4725433024454233
1103	68-69	0.460306239825222
1103	70-71	0.4520309892323908
1103	72-73	0.44427987312798933
1103	74-75	0.37942699825732973
1103	76-77	0.3802539384432606
1103	78-79	0.3785153989738035
1103	80-81	0.379117250713648
1103	82-83	0.3578821746266243
1103	84-85	0.3907114617788636
1103	86-87	0.36314081851214297
1103	88-89	0.3949099089205532
1103	90-91	0.43022946716013877
1103	92-93	0.3918918382659555
1103	94-95	0.34291391350233624
1103	96-97	0.3986398319249318
1103	98-99	0.4153840799568229
1103	100	0.628679676539349
1104	1	0.20495181320637812
1104	2	0.22450910581939354
1104	3	0.20961286108381927
1104	4	0.18770045117182121
1104	5	0.11990094210267443
1104	6	0.20862745249680614
1104	7	0.21856194225481573
1104	8	0.21007451773957087
1104	9	0.2331433180989393
1104	10-11	0.27503422855210147
1104	12-13	0.2562118588772364
1104	14-15	0.3008744643096364
1104	16-17	0.3120606838781157
1104	18-19	0.34101966448831433
1104	20-21	0.4100543220129538
1104	22-23	0.37178747857538497
1104	24-25	0.37550047631238215
1104	26-27	0.36624391472632567
1104	28-29	0.36085425442237096
1104	30-31	0.37183194390958363
1104	32-33	0.405171567107395
1104	34-35	0.49172414342189086
1104	36-37	0.4326306081159217
1104	38-39	0.3949279891323201
1104	40-41	0.41332992187524553
1104	42-43	0.433744925471899
1104	44-45	0.40530989840993215
1104	46-47	0.5085384295660234
1104	48-49	0.42629233804639455
1104	50-51	0.38911072109974754
1104	52-53	0.4173105874312526
1104	54-55	0.41868351640589907
1104	56-57	0.38782820411946517
1104	58-59	0.3933117774386119
1104	60-61	0.4016374756176475
1104	62-63	0.4176862447981833
1104	64-65	0.35507283877849005
1104	66-67	0.29548743148711765
1104	68-69	0.40019164325363477
1104	70-71	0.34045258047811444
1104	72-73	0.2863376228237371
1104	74-75	0.3351682403271994
1104	76-77	0.2867520616294428
1104	78-79	0.2780725812010729
1104	80-81	0.24545970158754926
1104	82-83	0.23589895886934187
1104	84-85	0.28267367158446177
1104	86-87	0.27081318465690885
1104	88-89	0.30455815541090203
1104	90-91	0.2931916013032776
1104	92-93	0.2674037461270231
1104	94-95	0.3207452605891348
1104	96-97	0.3250040966717407
1104	98-99	0.28541116455387794
1104	100	0.5367567086215175
1105	1	0.13255456015917844
1105	2	0.11174641813618535
1105	3	0.07007518223141318
1105	4	0.13364043841876594
1105	5	0.08868476866113895
1105	6	0.14650246339195405
1105	7	0.15674834786615577
1105	8	0.09694859738652184
1105	9	0.1340374081134783
1105	10-11	0.15450291246445147
1105	12-13	0.14788861024315025
1105	14-15	0.1997356349299082
1105	16-17	0.19207880422473522
1105	18-19	0.19098383666371177
1105	20-21	0.22830885095275022
1105	22-23	0.19840709350656027
1105	24-25	0.2052909225316526
1105	26-27	0.25972962717706594
1105	28-29	0.24995089780018276
1105	30-31	0.27291246706004557
1105	32-33	0.24003015959225849
1105	34-35	0.176206153817013
1105	36-37	0.21137167983120264
1105	38-39	0.2384593465068363
1105	40-41	0.21185474216268574
1105	42-43	0.1875228571834242
1105	44-45	0.19747658724917727
1105	46-47	0.2107874627652464
1105	48-49	0.22567890697023074
1105	50-51	0.20584970094717647
1105	52-53	0.17625639612626287
1105	54-55	0.17203500066872124
1105	56-57	0.11928296977063013
1105	58-59	0.19540754461738885
1105	60-61	0.19408427290372288
1105	62-63	0.21396272000082917
1105	64-65	0.20581531424721078
1105	66-67	0.24359341945887536
1105	68-69	0.17394833516645036
1105	70-71	0.24752671792667513
1105	72-73	0.22621963413633495
1105	74-75	0.23796689345789446
1105	76-77	0.14068986525993665
1105	78-79	0.17113707587277815
1105	80-81	0.05782887956605265
1105	82-83	0.08040175032020613
1105	84-85	0.09844140085271391
1105	86-87	0.08065757904739712
1105	88-89	0.11175775905699226
1105	90-91	0.12475151083916103
1105	92-93	0.1380594274291198
1105	94-95	0.10173008995969468
1105	96-97	0.1598230078912124
1105	98-99	0.12050527567058822
1105	100	0.2976081355414699
1106	1	0.13469416772778686
1106	2	0.21192558351015833
1106	3	0.1388122828990177
1106	4	0.1272942364683658
1106	5	0.09289769172609397
1106	6	0.13057401640307376
1106	7	0.15660123249939062
1106	8	0.12933729520692339
1106	9	0.12541519602658013
1106	10-11	0.17973894317800898
1106	12-13	0.13803792650671198
1106	14-15	0.21029627140450202
1106	16-17	0.199106285382328
1106	18-19	0.20567111928096438
1106	20-21	0.18468588313623258
1106	22-23	0.21380901350174497
1106	24-25	0.18912022663283068
1106	26-27	0.2346253762642263
1106	28-29	0.2041012588268174
1106	30-31	0.21773179464629777
1106	32-33	0.21475898524145975
1106	34-35	0.2626639385834366
1106	36-37	0.19509226493387644
1106	38-39	0.23581096054201822
1106	40-41	0.2474624857838421
1106	42-43	0.22379567822930824
1106	44-45	0.15354285103682486
1106	46-47	0.1644002834752527
1106	48-49	0.19685345222870865
1106	50-51	0.16404045771094644
1106	52-53	0.17690559880941237
1106	54-55	0.20160468556925082
1106	56-57	0.07267678799069444
1106	58-59	0.027478279142385986
1106	60-61	0.08935331706926775
1106	62-63	0.18509453780900031
1106	64-65	0.1452057984179831
1106	66-67	0.16420160944664985
1106	68-69	0.20653080029460824
1106	70-71	0.15556761925137153
1106	72-73	0.1555423324534928
1106	74-75	0.17159291070974092
1106	76-77	0.1159705768302004
1106	78-79	0.17180499059819
1106	80-81	0.08565491502612588
1106	82-83	0.12813130871776934
1106	84-85	0.15146660863417338
1106	86-87	0.13598551708994933
1106	88-89	0.18095561614365607
1106	90-91	0.16643618685212402
1106	92-93	0.2213481506172954
1106	94-95	0.2320097099204972
1106	96-97	0.1696655509611702
1106	98-99	0.18215905957788436
1106	100	0.3585764063878969
1107	1	0.11120415889550372
1107	2	0.1384953612322164
1107	3	0.09220105852521243
1107	4	0.11043859605661765
1107	5	0.025394867883711925
1107	6	0.01727629609203518
1107	7	0.11301907766965513
1107	8	0.1052305909906579
1107	9	0.10216937763626532
1107	10-11	0.14664166905453158
1107	12-13	0.05778095458624222
1107	14-15	0.12668313388268615
1107	16-17	0.11672872035518367
1107	18-19	0.1723259340114467
1107	20-21	0.16640943167327293
1107	22-23	0.12404161417684634
1107	24-25	0.10918514540506408
1107	26-27	0.17315008396625586
1107	28-29	0.22579934579830052
1107	30-31	0.11235420401781937
1107	32-33	0.15118521584668088
1107	34-35	0.15067175991983106
1107	36-37	0.09640217365586068
1107	38-39	0.0674947636309966
1107	40-41	0.09540824613841892
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2216	64-65	-0.6585345693462088
2216	66-67	-0.7257220384974943
2216	68-69	-0.6534490897741776
2216	70-71	-0.7778845517193531
2216	72-73	-0.7288756393376943
2216	74-75	-0.5853454127573343
2216	76-77	-0.7044583766982342
2216	78-79	-0.7059305174967818
2216	80-81	-0.674256419081587
2216	82-83	-0.706277366137563
2216	84-85	-0.646929618949585
2216	86-87	-0.6877397016452349
2216	88-89	-0.6521362388304723
2216	90-91	-0.6638460729293669
2216	92-93	-0.6586499035548812
2216	94-95	-0.6368562641799898
2216	96-97	-0.7066397675953162
2216	98-99	-0.7153480132612451
2216	100	-0.17792504692603828
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	5.0
12	30.0
13	72.0
14	336.0
15	928.0
16	2200.0
17	4491.0
18	7941.0
19	12734.0
20	18215.0
21	25810.0
22	36241.0
23	43278.0
24	43663.0
25	41408.0
26	41720.0
27	47545.0
28	58795.0
29	73282.0
30	91412.0
31	114131.0
32	144453.0
33	186643.0
34	264680.0
35	388028.0
36	619894.0
37	1166628.0
38	1050209.0
39	406270.0
40	1003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.67983558627479	21.652726699998304	13.720263227126303	24.947174486600606
2	14.159457566580686	20.80969652070207	40.10301868417211	24.927827228545134
3	17.067490534129973	26.987812264349763	29.98346909444284	25.96122810707742
4	11.618046122560969	17.41110001487455	38.98709433349231	31.983759529072174
5	12.229059890560965	38.90055226482546	35.30130486261247	13.569082982001104
6	29.629457770949465	39.29813021011449	17.355556886513966	13.716855132422074
7	25.75295663493081	32.87298080476725	23.789198307130267	17.584864253171677
8	26.413513028998686	34.75333137505368	19.52347574605242	19.309679849895208
9	26.1612465451593	14.34758727886822	21.43903301050337	38.05213316546911
10-11	25.461049143211774	26.002928425287646	27.507062991396857	21.02895944010373
12-13	25.850608029795403	23.96474469698673	28.15192261699131	22.032724656226556
14-15	23.09878944529505	24.875150781426242	26.165260225155347	25.86079954812336
16-17	21.29635250996055	27.324818291885467	27.500010731699536	23.878818466454444
18-19	20.74386243631756	27.001518996035813	30.58811577239548	21.666502795251148
20-21	22.2612038432158	26.289025314341703	29.902317004592554	21.547453837849947
22-23	22.611823798167187	25.887935709556466	29.65663262980399	21.84360786247235
24-25	22.072744978637786	25.934388637562705	30.457953303411312	21.534913080388197
26-27	22.0088249320479	26.208220727143168	29.42265091784649	22.360303422962442
28-29	21.16999441338384	26.729852869421382	29.96998803568811	22.130164681506663
30-31	22.129479896869388	26.28591823180839	29.81922298815895	21.765378883163272
32-33	22.5865276492529	26.516343151918555	29.058243284153534	21.838885914675014
34-35	22.377474142225477	26.419696532066624	29.77520257871497	21.427626746992924
36-37	22.135775827265835	25.91273104582558	29.473069464349194	22.47842366255939
38-39	22.311765479045693	26.007118898441124	30.092952871077127	21.588162751436055
40-41	21.87960504892735	25.406511668219185	30.484649683598835	22.229233599254627
42-43	22.083548222840776	25.55451713586679	29.60006124223204	22.761873399060395
44-45	22.328650019654344	26.526482052816725	29.18559278535436	21.95927514217458
46-47	22.415219062605466	26.378067758519997	28.386909043633864	22.819804135240673
48-49	23.266447249404084	26.30368174971265	28.19433125056597	22.2355397503173
50-51	22.687947320233302	26.339229226853618	27.94395558997876	23.028867862934323
52-53	22.076414197813634	26.895499206978506	27.75432134878453	23.273765246423327
54-55	22.09826598220909	26.890726155850032	27.625183230993805	23.385824630947074
56-57	23.224890020521052	26.336704722295302	27.340537676544123	23.097867580639523
58-59	22.10319686151894	26.727701772345654	28.2030348026021	22.96606656353331
60-61	22.274173868659126	27.296711459758477	27.441814258197333	22.987300413385068
62-63	22.674425378813662	26.537786109664886	27.10455271400593	23.68323579751552
64-65	21.850759753845274	26.75165498273217	27.507719164480292	23.88986609894226
66-67	22.479617656476883	26.355076169136378	27.488519361194985	23.676786813191754
68-69	22.102803958014324	26.202813994708556	27.840205607098373	23.854176440178747
70-71	22.05340549704933	25.966018771982846	27.913269853820232	24.06730587714759
72-73	22.65048032752889	25.966611178414507	28.217672165103767	23.165236328952837
74-75	22.766058479656486	26.4244711700224	27.666512810847692	23.14295753947342
76-77	22.50569496104287	26.36079111308075	27.257328429588938	23.87618549628744
78-79	22.209686004716225	26.46682943421651	27.42644798252184	23.89703657854543
80-81	21.918453801259965	26.89532545565263	27.919282901704378	23.26693784138303
82-83	22.66749576711108	25.88313199642931	28.917647799521223	22.53172443693839
84-85	23.045364018226312	26.401871690165002	27.41691671526593	23.135847576342755
86-87	21.648853067497893	27.073645419332472	27.822462530526575	23.45503898264306
88-89	22.565943227469717	25.8946302459358	27.665207359942634	23.874219166651848
90-91	22.140610202391677	25.88842630153541	27.966369511016755	24.00459398505616
92-93	22.557357867838203	26.21091898302736	27.585843886682248	23.645879262452198
94-95	21.421412581926305	26.174686721249113	28.21415934304828	24.189741353776302
96-97	21.838395322696073	26.017257799339294	27.760872795836672	24.38347408212796
98-99	21.168216017460168	26.7061307031062	27.65533419636639	24.47031908306724
100	21.984861076537943	25.98863527352394	26.815841400273715	25.210662249664402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1004.0
1	666.0
2	442.5
3	548.5
4	386.0
5	313.5
6	623.0
7	1472.5
8	2499.5
9	2751.5
10	2033.5
11	1363.0
12	1262.5
13	1356.0
14	1625.5
15	2257.5
16	2942.0
17	3718.0
18	4967.0
19	6193.0
20	7741.0
21	9518.5
22	11532.5
23	13820.0
24	15773.5
25	17735.0
26	20739.5
27	24147.5
28	28721.5
29	38016.0
30	50603.0
31	61490.5
32	76633.5
33	91399.5
34	100819.5
35	112714.5
36	124511.0
37	145367.0
38	153279.5
39	141412.5
40	131292.0
41	122589.0
42	121573.5
43	128442.5
44	141105.5
45	170092.5
46	196137.5
47	212383.0
48	223562.5
49	218039.5
50	207536.0
51	212186.0
52	215260.0
53	207316.0
54	201265.0
55	174451.5
56	150181.5
57	124941.5
58	97905.0
59	95108.0
60	78751.0
61	53476.0
62	39371.5
63	31669.0
64	22702.5
65	10977.0
66	6046.0
67	4179.5
68	3041.0
69	2181.0
70	1569.5
71	1031.0
72	706.5
73	487.5
74	267.5
75	176.5
76	94.5
77	25.0
78	9.0
79	6.5
80	4.5
81	4.0
82	4.0
83	1.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006439019723637274
3	0.0
4	0.092108644046697
5	0.00394517716400633
6	4.088266491198269E-5
7	2.0441332455991345E-5
8	0.0
9	0.0
10-11	0.0
12-13	3.8838531666383555E-4
14-15	0.009167937606512118
16-17	0.0
18-19	4.088266491198269E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.0441332455991345E-5
60-61	0.0
62-63	0.0
64-65	8.176532982396538E-5
66-67	1.7375132587592643E-4
68-69	0.0
70-71	5.00812645171788E-4
72-73	4.599299802598053E-4
74-75	4.701506464878009E-4
76-77	1.0220666227995674E-4
78-79	2.0441332455991345E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0625913599802455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4892049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.83992934778343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29003586247764	44.70410710284348
2	16.23611761797811	20.080807327561363
3	5.653836163797524	10.488984867395452
4	2.501406486308416	6.187472015335985
5	1.2578814314666684	3.889364942489373
6	0.6896049526870666	2.5587072931229837
7	0.39574611919360025	1.7131038435414199
8	0.25949297933346355	1.2837622006581768
9	0.1709853682850796	0.9516350784828644
>10	0.5295694009013836	4.848898070391035
>50	0.00785676445367597	0.33208529353295907
>100	0.005901513267911407	0.7109295543509057
>500	7.589526594376431E-4	0.33874310408279196
>1k	6.166490275823381E-4	0.5962324442235336
>5k	9.486908116651341E-5	0.42304754061752403
>10k+	9.486908116651341E-5	0.8921193213701815
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16564	0.3385902308010406	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13849	0.2830920131830241	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9353	0.19118778246088705	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5069	0.10361711421942013	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0220666227995672E-4
2	0.0	0.0	0.0	0.0	1.0220666227995672E-4
3	0.0	0.0	0.0	0.0	1.0220666227995672E-4
4	0.0	0.0	0.0	0.0	1.0220666227995672E-4
5	0.0	0.0	0.0	0.0	1.0220666227995672E-4
6	0.0	0.0	0.0	0.0	1.6353065964793076E-4
7	0.0	0.0	0.0	0.0	1.6353065964793076E-4
8	0.0	0.0	0.0	0.0	1.6353065964793076E-4
9	0.0	0.0	0.0	2.0441332455991345E-5	1.6353065964793076E-4
10-11	0.0	0.0	0.0	6.132399736797404E-5	1.839719921039221E-4
12-13	0.0	0.0	0.0	6.132399736797404E-5	5.62136642539762E-4
14-15	0.0	0.0	0.0	6.132399736797404E-5	6.54122638591723E-4
16-17	0.0	0.0	0.0	6.132399736797404E-5	6.950053035037057E-4
18-19	0.0	0.0	0.0	6.132399736797404E-5	7.15446635959697E-4
20-21	0.0	0.0	0.0	7.154466359596971E-5	8.585359631516365E-4
22-23	0.0	0.0	0.0	1.4308932719193942E-4	8.789772956076278E-4
24-25	0.0	0.0	0.0	2.5551665569989186E-4	8.789772956076278E-4
26-27	0.0	0.0	0.0	5.92798641223749E-4	9.403012929756018E-4
28-29	0.0	0.0	0.0	0.002544945890770922	9.607426254315932E-4
30-31	0.0	0.0	0.0	0.009617646920543926	9.709632916595889E-4
32-33	0.0	0.0	0.0	0.022495686367818474	0.001001625290343576
34-35	0.0	0.0	0.0	0.04247708884355002	0.00106294928771155
36-37	0.0	0.0	0.0	0.07624617006084772	0.0010731699539395455
38-39	0.0	0.0	0.0	0.12673626122714632	0.0011447146175355154
40-41	0.0	0.0	0.0	0.18223447884516283	0.0011447146175355154
42-43	0.0	0.0	0.0	0.24088066166140198	0.0011447146175355154
44-45	0.0	0.0	0.0	0.31215958793544385	0.0011855972824474982
46-47	0.0	0.0	0.0	0.4029191040400454	0.0012264799473594807
48-49	0.0	0.0	0.0	0.5086416755024326	0.0012571419460434678
50-51	0.0	0.0	0.0	0.6214369479945929	0.0012673626122714633
52-53	0.0	0.0	0.0	0.7342117791542971	0.0013286866096394374
54-55	0.0	0.0	0.0	0.8606618617270596	0.00136956927455142
56-57	0.0	0.0	0.0	1.0146770811167263	0.001400231273235407
58-59	0.0	0.0	0.0	1.2037900683333302	0.001430893271919394
60-61	0.0	0.0	0.0	1.3987901592972598	0.0014513346043753855
62-63	0.0	0.0	0.0	1.6054213684286482	0.0015126586017433594
64-65	0.0	0.0	0.0	1.8250532650020472	0.0015433206004273467
66-67	0.0	0.0	0.0	2.0715246310901625	0.0015944239315673248
68-69	0.0	0.0	0.0	2.3516526510670683	0.0016148652640233163
70-71	0.0	0.0	0.0	2.6624222283955046	0.0016353065964793076
72-73	0.0	0.0	0.0	2.9907917929685497	0.0016966305938472814
74-75	0.0	0.0	0.0	3.3194168741972945	0.0017988372561272383
76-77	0.0	0.0	0.0	3.6917864068818607	0.001839719921039221
78-79	0.0	0.0	0.0	4.101389826635016	0.0018806025859512037
80-81	0.0	0.0	0.0	4.549116331418594	0.0019112645846351907
82-83	0.0	0.0	0.0	5.025399377643192	0.002085015910511117
84-85	0.0	0.0	0.0	5.484797883259141	0.0021054572429671085
86-87	0.0	0.0	0.0	5.97510368354855	0.0022178845714750607
88	0.0	0.0	0.0	6.372197007838638	0.0023303118999830133
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10040	0.0	48.72781	1
GTATCAA	18535	0.0	41.952473	1
GTGGTAT	2420	0.0	41.015156	1
GGGCACC	5035	0.0	40.62629	94
CAACGCA	22900	0.0	32.98123	5
ATCAACG	22940	0.0	32.891174	3
CTGTGCG	6905	0.0	32.33708	9
TATCAAC	24685	0.0	31.519526	2
TAGTACT	7215	0.0	31.099287	4
GTACTAG	5195	0.0	31.058884	1
AACGCAG	26030	0.0	30.06137	6
ACTGTGC	7680	0.0	29.924692	8
GTACTGT	7825	0.0	28.768677	6
TCAACGC	27130	0.0	28.123507	4
TACTGTG	8515	0.0	28.038336	7
ACGCAGA	27330	0.0	27.599966	7
TAGGCAT	6720	0.0	27.485888	5
CGCAGAG	27565	0.0	27.348179	8
CTAGTAC	8430	0.0	26.95181	3
GCAGAGT	29235	0.0	25.630438	9
>>END_MODULE