FastQCFastQC Report
Mon 16 Jan 2017
ERR1146680_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146680_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173671
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC52950.040.4764394
GTACTAG55250.035.999781
CAACGCA103200.032.694615
GGTATCA78000.031.9501551
TAGGCAT69700.031.755545
GTATCAA120600.031.503281
ATCAACG109100.031.0159663
TAGTACT75800.030.752594
GCATCGT60250.030.6516848
CTGTGCG76150.030.175739
CTAGGCA79600.028.871214
ACTGTGC85300.028.5364258
CTAGTAC88000.027.6112123
GTACTGT86050.027.1411676
TACTGTG91900.026.9472187
AACGCAG132100.026.8220236
TATCAAC142900.026.4095442
ACGCAGA127100.026.1392237
GTACATG218500.025.7378031
CGCAGAG128800.025.61188
Produced by FastQC (version 0.11.5)