##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146680_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5173671 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38193653983796 31.0 31.0 34.0 28.0 34.0 2 31.49968388016942 33.0 31.0 34.0 28.0 34.0 3 31.63356637868933 33.0 31.0 34.0 28.0 34.0 4 35.0347903838493 37.0 35.0 37.0 32.0 37.0 5 35.216235628434816 37.0 35.0 37.0 32.0 37.0 6 35.26701562584865 37.0 35.0 37.0 32.0 37.0 7 35.17788065766068 37.0 35.0 37.0 32.0 37.0 8 35.18787046953701 37.0 35.0 37.0 32.0 37.0 9 36.6826904919157 39.0 37.0 39.0 32.0 39.0 10-11 36.518612606019985 39.0 37.0 39.0 32.0 39.0 12-13 36.46367791844514 39.0 37.0 39.0 32.0 39.0 14-15 37.66494545169185 40.0 38.0 41.0 31.5 41.0 16-17 37.700396005080336 40.0 38.0 41.0 31.5 41.0 18-19 37.66944912036347 40.0 37.5 41.0 31.0 41.0 20-21 37.733815505469906 40.0 38.0 41.0 31.0 41.0 22-23 37.68947919185429 40.0 37.5 41.0 31.0 41.0 24-25 37.65802174123557 40.0 37.0 41.0 31.0 41.0 26-27 37.69411584153689 40.0 37.0 41.0 31.0 41.0 28-29 37.687295829209084 40.0 37.0 41.0 31.0 41.0 30-31 37.55633929950319 40.0 37.0 41.0 31.0 41.0 32-33 37.548942713983934 40.0 37.0 41.0 31.0 41.0 34-35 37.49392868236113 40.0 37.0 41.0 31.0 41.0 36-37 37.38399204356055 40.0 36.5 41.0 30.5 41.0 38-39 37.274659231327234 40.0 36.0 41.0 30.0 41.0 40-41 37.181873470500925 40.0 36.0 41.0 30.0 41.0 42-43 37.016778995030805 40.0 35.5 41.0 30.0 41.0 44-45 36.85658809769697 39.0 35.0 41.0 30.0 41.0 46-47 36.83906031133406 39.0 35.0 41.0 30.0 41.0 48-49 36.694151889441756 39.0 35.0 41.0 30.0 41.0 50-51 36.51309582692831 39.0 35.0 41.0 29.0 41.0 52-53 36.29178884780265 39.0 35.0 41.0 29.0 41.0 54-55 36.066460159526954 38.0 35.0 40.0 29.0 41.0 56-57 35.8178198806998 38.0 34.0 40.0 28.5 41.0 58-59 35.51495997716128 37.0 34.0 40.0 28.0 41.0 60-61 35.25606160499962 37.0 34.0 40.0 28.0 41.0 62-63 34.93989007031951 36.0 34.0 40.0 27.5 41.0 64-65 34.78254976012197 36.0 34.0 39.0 28.0 41.0 66-67 34.52152533471881 35.0 33.0 39.0 27.5 41.0 68-69 34.389468039231716 35.0 33.0 39.0 27.0 41.0 70-71 34.29422425585237 35.0 33.0 38.5 28.0 41.0 72-73 34.071863866102035 35.0 33.0 37.0 27.0 40.0 74-75 33.82306654211294 35.0 33.0 37.0 27.0 39.5 76-77 33.566405266202665 35.0 33.0 36.5 27.0 39.0 78-79 33.320914298570585 35.0 33.0 36.0 26.0 39.0 80-81 33.137116662424035 35.0 33.0 36.0 26.0 38.5 82-83 32.88724920854071 35.0 33.0 35.0 26.0 37.0 84-85 32.71524793517021 35.0 33.0 35.0 26.0 37.0 86-87 32.55119324363687 35.0 33.0 35.0 26.0 36.5 88-89 32.42954016596726 35.0 33.0 35.0 25.0 36.0 90-91 32.28679084928284 35.0 32.5 35.0 25.0 36.0 92-93 32.13641261688268 35.0 32.0 35.0 25.0 36.0 94-95 32.064960450712846 35.0 32.0 35.0 25.0 35.5 96-97 31.959586142992084 35.0 32.0 35.0 25.0 35.0 98-99 31.870988994081767 35.0 32.0 35.0 25.0 35.0 100 30.940553235797175 34.0 30.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32869282935095967 1101 2 0.42938574172085353 1101 3 0.50452828910052 1101 4 0.5186288800811809 1101 5 0.3824081925049043 1101 6 0.45112299855732374 1101 7 0.4071615868955476 1101 8 0.4202506241437618 1101 9 0.6348878663841973 1101 10-11 0.641981502321535 1101 12-13 0.5880245952795065 1101 14-15 0.7460308601997596 1101 16-17 0.7033216259430759 1101 18-19 0.6946952011309833 1101 20-21 0.671122495827241 1101 22-23 0.6486128208435602 1101 24-25 0.7279740014855278 1101 26-27 0.6827519229037478 1101 28-29 0.692571146614597 1101 30-31 0.6915878120429539 1101 32-33 0.7329127853916759 1101 34-35 0.7236604518475431 1101 36-37 0.721887223271878 1101 38-39 0.6910153523089733 1101 40-41 0.686436293367386 1101 42-43 0.692602197058271 1101 44-45 0.7076415112037964 1101 46-47 0.6504842210919364 1101 48-49 0.6256687397364757 1101 50-51 0.5943946172354302 1101 52-53 0.5725681157934801 1101 54-55 0.6327977870800723 1101 56-57 0.6058785490024263 1101 58-59 0.569206088231077 1101 60-61 0.5779441365377096 1101 62-63 0.5664937654681452 1101 64-65 0.5587199970770129 1101 66-67 0.5685597144556311 1101 68-69 0.5732897250331703 1101 70-71 0.5730885891737145 1101 72-73 0.5449137946906859 1101 74-75 0.5277382407885156 1101 76-77 0.5450633222221839 1101 78-79 0.5370372452975545 1101 80-81 0.4835924799090563 1101 82-83 0.5278604418117965 1101 84-85 0.5073259717142662 1101 86-87 0.5210230293618991 1101 88-89 0.5019577761620084 1101 90-91 0.4706118154313117 1101 92-93 0.4181049256037639 1101 94-95 0.3822094737492705 1101 96-97 0.42035261987645 1101 98-99 0.4768968857989755 1101 100 0.5046519078495137 1102 1 0.281490681301225 1102 2 0.3370743724830767 1102 3 0.3854308504852284 1102 4 0.4474505908818287 1102 5 0.36868731902831087 1102 6 0.3648038835115699 1102 7 0.3841546321825007 1102 8 0.4222885219216508 1102 9 0.5158110073115552 1102 10-11 0.6312773354836025 1102 12-13 0.5462048453431407 1102 14-15 0.715112730688638 1102 16-17 0.6153685024257243 1102 18-19 0.654795913913631 1102 20-21 0.6636580468068018 1102 22-23 0.650089326373724 1102 24-25 0.6209124569834614 1102 26-27 0.6302777505454031 1102 28-29 0.6114352265632164 1102 30-31 0.6798680330517328 1102 32-33 0.5592537847339045 1102 34-35 0.6003950876993898 1102 36-37 0.6581890477141741 1102 38-39 0.6470223593817153 1102 40-41 0.5428725832066377 1102 42-43 0.6107860789332094 1102 44-45 0.5705309576301474 1102 46-47 0.5119742405818997 1102 48-49 0.49302139476531437 1102 50-51 0.5293899383203993 1102 52-53 0.4824805726555752 1102 54-55 0.4667542723315492 1102 56-57 0.48645269443422023 1102 58-59 0.4209175423373708 1102 60-61 0.4452247270944696 1102 62-63 0.49975839391118626 1102 64-65 0.49925892569881114 1102 66-67 0.5261041450298762 1102 68-69 0.4682416479930325 1102 70-71 0.45935028170419656 1102 72-73 0.450050481000865 1102 74-75 0.42058644209591023 1102 76-77 0.4234995489700637 1102 78-79 0.3874449285624877 1102 80-81 0.4422186982211329 1102 82-83 0.4521086603872533 1102 84-85 0.4369931391858728 1102 86-87 0.46860623082051944 1102 88-89 0.44788057286449856 1102 90-91 0.460525359528134 1102 92-93 0.4493067137189186 1102 94-95 0.5019406576669141 1102 96-97 0.4998758955826723 1102 98-99 0.4653204421499204 1102 100 0.5238926258941063 1103 1 0.31955749944622625 1103 2 0.3710494485927178 1103 3 0.40294151862404703 1103 4 0.5001087798773369 1103 5 0.39819855475413846 1103 6 0.4298439952263351 1103 7 0.39838328326813155 1103 8 0.3844489821786894 1103 9 0.513966958540486 1103 10-11 0.638599025163316 1103 12-13 0.49861785136796044 1103 14-15 0.6705016709026097 1103 16-17 0.6422940504790944 1103 18-19 0.6534926561956382 1103 20-21 0.5729002146237647 1103 22-23 0.653761102744447 1103 24-25 0.6680397785635179 1103 26-27 0.6081264496323371 1103 28-29 0.5948906134328382 1103 30-31 0.5821357623809362 1103 32-33 0.5433665859523913 1103 34-35 0.5737105205240667 1103 36-37 0.6222157852033519 1103 38-39 0.6432592331709444 1103 40-41 0.5926164078052025 1103 42-43 0.588574608925498 1103 44-45 0.5356708233702463 1103 46-47 0.5584115054702536 1103 48-49 0.5489521613305399 1103 50-51 0.5738862627073331 1103 52-53 0.5414667477707837 1103 54-55 0.4765245031238692 1103 56-57 0.5575109945111336 1103 58-59 0.5121330981189303 1103 60-61 0.4500073784200467 1103 62-63 0.4515330472537684 1103 64-65 0.4373208109524427 1103 66-67 0.4800299653057607 1103 68-69 0.517233724307907 1103 70-71 0.4885712076383655 1103 72-73 0.49330274236616134 1103 74-75 0.42904659438218573 1103 76-77 0.39840653960792594 1103 78-79 0.41291247226881467 1103 80-81 0.38051468266487376 1103 82-83 0.42303639109279345 1103 84-85 0.49919348887306114 1103 86-87 0.47970474918701456 1103 88-89 0.4865484120571111 1103 90-91 0.5204116350672692 1103 92-93 0.4675353916004994 1103 94-95 0.47921933375195636 1103 96-97 0.5002646805019104 1103 98-99 0.5107971939201299 1103 100 0.5826063348864032 1104 1 0.19551789888745574 1104 2 0.30859202986161094 1104 3 0.31906852289832344 1104 4 0.467273694326245 1104 5 0.3379142143848384 1104 6 0.2736402990810163 1104 7 0.3141987790070644 1104 8 0.3752062330302266 1104 9 0.44577438460812147 1104 10-11 0.6190746112208103 1104 12-13 0.48682838062571676 1104 14-15 0.5667452569778249 1104 16-17 0.6323308302657864 1104 18-19 0.5527110218213522 1104 20-21 0.5658662124931908 1104 22-23 0.6158637776891496 1104 24-25 0.627428900424384 1104 26-27 0.5316750650903117 1104 28-29 0.5260747668015853 1104 30-31 0.5076805273964098 1104 32-33 0.545106468230486 1104 34-35 0.5823395574686501 1104 36-37 0.5907868081780236 1104 38-39 0.5708585247058906 1104 40-41 0.5805347966249101 1104 42-43 0.6045308450352991 1104 44-45 0.5411322092807396 1104 46-47 0.5583060758759686 1104 48-49 0.5477844090676811 1104 50-51 0.3976524092501208 1104 52-53 0.48980278707072245 1104 54-55 0.5437858149703416 1104 56-57 0.54851058090469 1104 58-59 0.5389968535665872 1104 60-61 0.49025844281329967 1104 62-63 0.4796284534955575 1104 64-65 0.48400996551667674 1104 66-67 0.4944604844508689 1104 68-69 0.46842459388178526 1104 70-71 0.47961042492523376 1104 72-73 0.47300242012123306 1104 74-75 0.42917535431099196 1104 76-77 0.39095201203186747 1104 78-79 0.4023308107297652 1104 80-81 0.38818002682479147 1104 82-83 0.4324772776659529 1104 84-85 0.49303051430142375 1104 86-87 0.39353331502584865 1104 88-89 0.3633051870993427 1104 90-91 0.3953325309180329 1104 92-93 0.3847198019608058 1104 94-95 0.4940134158566636 1104 96-97 0.4456965873673546 1104 98-99 0.4231204163625186 1104 100 0.4838699221647751 1105 1 0.15086430010061136 1105 2 0.27323923665310446 1105 3 0.2708523795279092 1105 4 0.36246138460310107 1105 5 0.24661619530137813 1105 6 0.2599581761562746 1105 7 0.34587700640011576 1105 8 0.3244905660327646 1105 9 0.4925129818994094 1105 10-11 0.4617107817922914 1105 12-13 0.3595002356803576 1105 14-15 0.536732488952147 1105 16-17 0.42825694102132417 1105 18-19 0.4239263430929512 1105 20-21 0.45550273186712786 1105 22-23 0.45735985450139083 1105 24-25 0.417572364100252 1105 26-27 0.4686266603767564 1105 28-29 0.4710478955090238 1105 30-31 0.5563123683206754 1105 32-33 0.49189421284163615 1105 34-35 0.4326642909883347 1105 36-37 0.4663292405600856 1105 38-39 0.44895567644965695 1105 40-41 0.46158616743982606 1105 42-43 0.4710751669773998 1105 44-45 0.3985409636136197 1105 46-47 0.46995620582958253 1105 48-49 0.34237043922846055 1105 50-51 0.4165850617728708 1105 52-53 0.46722754866556215 1105 54-55 0.4814670117242983 1105 56-57 0.4635849168704027 1105 58-59 0.45443814388087844 1105 60-61 0.4827242000879721 1105 62-63 0.46699171516801385 1105 64-65 0.4290476777238297 1105 66-67 0.4177378644871226 1105 68-69 0.35726020967543803 1105 70-71 0.3876716846405728 1105 72-73 0.361369104993571 1105 74-75 0.3773009344357945 1105 76-77 0.383973917471117 1105 78-79 0.34898204609478967 1105 80-81 0.27957175891950214 1105 82-83 0.3125570771612587 1105 84-85 0.32747292878603673 1105 86-87 0.3204689965122327 1105 88-89 0.3041119358730029 1105 90-91 0.3544912960356186 1105 92-93 0.36647283878223647 1105 94-95 0.36215437295768993 1105 96-97 0.30134709396242343 1105 98-99 0.2806758909029803 1105 100 0.40352503846014187 1106 1 0.0937177320509619 1106 2 0.22127397233678536 1106 3 0.1969322135027376 1106 4 0.36134691549819564 1106 5 0.26229627283937873 1106 6 0.23310058799031452 1106 7 0.24950824840470887 1106 8 0.23658506966107495 1106 9 0.31497721554129043 1106 10-11 0.37643992948878235 1106 12-13 0.2967261572820661 1106 14-15 0.44779841301183154 1106 16-17 0.3179014561015734 1106 18-19 0.3682741133168008 1106 20-21 0.389395720487812 1106 22-23 0.35312207874709856 1106 24-25 0.3994678274568457 1106 26-27 0.4052398334574434 1106 28-29 0.4002599044024322 1106 30-31 0.4597393608182614 1106 32-33 0.3535362950833161 1106 34-35 0.3232780310796173 1106 36-37 0.36020433901882143 1106 38-39 0.4109865659580052 1106 40-41 0.40453840727135315 1106 42-43 0.36771199808645605 1106 44-45 0.39136080786725813 1106 46-47 0.45029308833269255 1106 48-49 0.331898549546942 1106 50-51 0.3380851679127872 1106 52-53 0.30331330413036994 1106 54-55 0.42208068198668514 1106 56-57 0.3537328982568084 1106 58-59 0.39656186391729875 1106 60-61 0.34542768147717595 1106 62-63 0.348739280932854 1106 64-65 0.3350468174318877 1106 66-67 0.34374542555946874 1106 68-69 0.36021501921015187 1106 70-71 0.3836125311901881 1106 72-73 0.2706634700813595 1106 74-75 0.3192504313884541 1106 76-77 0.27416903335913645 1106 78-79 0.2490045309977802 1106 80-81 0.2918075263948836 1106 82-83 0.26021054709852365 1106 84-85 0.23899832695037304 1106 86-87 0.2717270338176263 1106 88-89 0.25667856660106736 1106 90-91 0.24095161367327478 1106 92-93 0.2211142255281473 1106 94-95 0.24012489399294168 1106 96-97 0.2726564974002379 1106 98-99 0.3205611061714855 1106 100 0.22725038187862978 1107 1 0.08026606564552807 1107 2 0.12791508434598953 1107 3 0.12038195396084816 1107 4 0.20704818570143857 1107 5 0.12044384957762588 1107 6 0.15916316283846754 1107 7 0.20618890380048782 1107 8 0.17135794617609434 1107 9 0.24192990075237475 1107 10-11 0.2973135233167312 1107 12-13 0.21263749148207012 1107 14-15 0.35030501296695604 1107 16-17 0.23984539771667812 1107 18-19 0.20237641697517006 1107 20-21 0.17445878245721502 1107 22-23 0.29752776520901847 1107 24-25 0.33460623699315306 1107 26-27 0.27080019449060444 1107 28-29 0.2918953384525622 1107 30-31 0.40700835726651974 1107 32-33 0.37630754888526496 1107 34-35 0.39671738627654207 1107 36-37 0.4100033950997499 1107 38-39 0.39406819436695883 1107 40-41 0.35376747770467887 1107 42-43 0.48169484947195684 1107 44-45 0.4324120421638682 1107 46-47 0.4024085826813675 1107 48-49 0.4213973473665149 1107 50-51 0.34559847538924515 1107 52-53 0.3383569657660104 1107 54-55 0.3398903676577376 1107 56-57 0.3458696804700736 1107 58-59 0.3421517390687967 1107 60-61 0.30793176643194187 1107 62-63 0.335933011769427 1107 64-65 0.33361368980611417 1107 66-67 0.3637159242466481 1107 68-69 0.3283276574199263 1107 70-71 0.2958607878492998 1107 72-73 0.32305717674113765 1107 74-75 0.3730775217840261 1107 76-77 0.3813318030557937 1107 78-79 0.34193206569300827 1107 80-81 0.316833252646056 1107 82-83 0.23165581690017945 1107 84-85 0.20150516508782346 1107 86-87 0.1778508676206627 1107 88-89 0.2744245577109794 1107 90-91 0.27072369880834657 1107 92-93 0.2769409589063585 1107 94-95 0.22821923570312208 1107 96-97 0.29802630745880876 1107 98-99 0.25479006903860935 1107 100 0.3446163825568078 1108 1 0.06481656948202996 1108 2 0.13109067267162544 1108 3 0.08128126183246209 1108 4 0.19605337637863585 1108 5 0.1180149655249707 1108 6 0.10760917226569688 1108 7 0.1909664473853283 1108 8 0.12890581467537743 1108 9 0.15895333282895052 1108 10-11 0.3527363083427275 1108 12-13 0.2051740756106497 1108 14-15 0.26266500353489874 1108 16-17 0.3240672716758439 1108 18-19 0.30354317970200384 1108 20-21 0.2484164170052452 1108 22-23 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2215 40-41 -0.9998501072326107 2215 42-43 -0.999828601294233 2215 44-45 -0.9156505160190278 2215 46-47 -1.074246293196289 2215 48-49 -1.1218848364180616 2215 50-51 -1.0059797390896748 2215 52-53 -0.9497814154146695 2215 54-55 -0.9195971672874492 2215 56-57 -0.9296803672815628 2215 58-59 -0.973809367989162 2215 60-61 -0.9126137277212294 2215 62-63 -0.8926276753435829 2215 64-65 -0.8746993925103226 2215 66-67 -0.8005267355795169 2215 68-69 -0.7937964766164569 2215 70-71 -0.9516182485816103 2215 72-73 -0.8378467683873723 2215 74-75 -0.929957808751567 2215 76-77 -0.876935220383281 2215 78-79 -0.7812566425881258 2215 80-81 -0.8008437669431103 2215 82-83 -0.7846057939782582 2215 84-85 -0.7865917125864677 2215 86-87 -0.7179365574993213 2215 88-89 -0.752615468579009 2215 90-91 -0.8823296316464173 2215 92-93 -0.8379948617658819 2215 94-95 -0.8514988701417181 2215 96-97 -1.1057611999060093 2215 98-99 -0.9326155886966951 2215 100 -0.43109115340935134 2216 1 -0.45518844424028515 2216 2 -0.6909318226155925 2216 3 -0.7042865930096696 2216 4 -0.5229607335719706 2216 5 -0.5410092731689531 2216 6 -0.45536294645208386 2216 7 -0.5380608831696847 2216 8 -0.5981334869084307 2216 9 -0.751995791986964 2216 10-11 -1.1309903668604164 2216 12-13 -0.7412014868573564 2216 14-15 -0.8605714847817225 2216 16-17 -0.815985818966837 2216 18-19 -1.0830302095377036 2216 20-21 -1.1568864525671216 2216 22-23 -1.074633808532056 2216 24-25 -1.1031945979745856 2216 26-27 -1.4449843489840788 2216 28-29 -1.31571150971142 2216 30-31 -1.4110611273082014 2216 32-33 -1.6110924988388717 2216 34-35 -1.264309250299796 2216 36-37 -1.354241197491234 2216 38-39 -1.2566077857863505 2216 40-41 -1.233527721355948 2216 42-43 -1.204544401791324 2216 44-45 -1.086608319757083 2216 46-47 -1.246302160345742 2216 48-49 -1.2479157855527134 2216 50-51 -1.3161145369230312 2216 52-53 -1.332252572510889 2216 54-55 -1.3027816394267262 2216 56-57 -1.279099837952856 2216 58-59 -1.2745353491171443 2216 60-61 -1.2687149837087546 2216 62-63 -1.380087138372808 2216 64-65 -1.3477613898668181 2216 66-67 -1.2901953397879566 2216 68-69 -1.3025075457534356 2216 70-71 -1.2040880303981467 2216 72-73 -1.1719015343703987 2216 74-75 -1.262843128756522 2216 76-77 -1.2327531195278212 2216 78-79 -1.0809432034661484 2216 80-81 -1.1677984593858426 2216 82-83 -1.1400597363017724 2216 84-85 -1.1774824905558887 2216 86-87 -1.121464314843422 2216 88-89 -1.2021074713748163 2216 90-91 -1.2607467381564348 2216 92-93 -1.2314610944600624 2216 94-95 -1.2585627354904219 2216 96-97 -1.2677898720258192 2216 98-99 -1.3865505016220112 2216 100 -0.9599342666780366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 29.0 4 40.0 5 34.0 6 62.0 7 222.0 8 570.0 9 902.0 10 1326.0 11 2483.0 12 4172.0 13 6128.0 14 8731.0 15 11583.0 16 14102.0 17 16561.0 18 19313.0 19 21862.0 20 25380.0 21 28882.0 22 33653.0 23 39130.0 24 44874.0 25 51971.0 26 58164.0 27 63637.0 28 73445.0 29 88961.0 30 104174.0 31 121251.0 32 149941.0 33 195701.0 34 285201.0 35 416401.0 36 675814.0 37 1199502.0 38 1014379.0 39 394083.0 40 997.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.981016990193154 20.708660610211137 12.369639639413133 23.94068276018258 2 15.759285236695467 19.730759498833724 40.2605845058457 24.249370758625115 3 18.46477163754045 26.5343188830162 29.029771969051023 25.97113751039232 4 12.212203466103867 16.551251008566094 39.80504470552576 31.431500819804274 5 13.116504512821345 38.60548852784256 35.02163533187126 13.25637162746483 6 30.178865195558725 38.34534859065158 17.91694347471693 13.558842739072757 7 27.00168276692808 31.974098610219304 23.533880132719396 17.49033849013322 8 26.446094571930185 34.835889158183065 19.80175533240155 18.9162609374852 9 26.249167415010614 15.052614732434341 21.235739747172495 37.462478105382544 10-11 25.709054261863766 25.831965505217592 27.499134073957816 20.959846158960826 12-13 25.756864843206305 23.818798350066004 27.468321999806133 22.95601480692156 14-15 23.25739935415173 25.074748809402088 24.796154985802126 26.87169685064406 16-17 22.279832605380772 27.600483837017748 26.05227109794781 24.067412459653674 18-19 21.731577850703825 28.27977791789132 27.6583322734616 22.33031195794326 20-21 23.50186247895693 27.384379453788167 26.60172562108645 22.512032446168448 22-23 23.995502169183773 26.95868741428849 26.34842629075857 22.69738412576917 24-25 23.439611528319457 26.996423675356123 27.110251405813063 22.45371339051136 26-27 23.21368754147343 27.27708339414009 26.08372138514539 23.425507679241086 28-29 22.296380457843398 27.789528966233895 26.74260148697045 23.171489088952253 30-31 23.099477686597076 27.4162588373489 26.69487041132217 22.789393064731854 32-33 23.53664222356929 27.655809281985306 25.723405760571044 23.084142733874362 34-35 23.470789424826872 27.39247137500581 26.75191787678522 22.3848213233821 36-37 23.105746528091554 27.031453887544853 26.39350167490726 23.469297909456326 38-39 23.312558979975513 27.097657501904948 26.944314398014757 22.64546912010478 40-41 22.89764174950598 26.27883300031372 27.61630767107469 23.207217579105606 42-43 22.87903331271009 26.530932788938955 26.895259595294036 23.69477430305692 44-45 23.064388270398275 27.38719155577865 26.766767707260087 22.78165246656299 46-47 23.01455114766246 27.166682116207546 26.304046771221557 23.514719964908437 48-49 23.75705085859192 26.794606268379304 26.50976869065964 22.938574182369127 50-51 23.282207921820895 26.632754900089168 26.519719974191613 23.565317203898324 52-53 22.769067982161477 27.252479071131567 26.359866530069887 23.618586416637072 54-55 22.72351086197524 27.18448732234699 26.38013356212317 23.71186825355461 56-57 23.888240746737086 26.5365012502646 26.09991871105307 23.47533929194524 58-59 22.753833037877044 26.970184368590257 26.981261562908994 23.294721030623705 60-61 22.745264822455308 27.63638630716805 26.276759292865975 23.341589577510668 62-63 23.19834374453303 26.913484428627626 25.88375669205111 24.004415134788225 64-65 22.359937774285356 27.121606424771212 26.362207205443926 24.15624859549951 66-67 23.113424886648957 26.707456835554215 26.32629344970214 23.852824828094686 68-69 22.548472882865607 26.74187885432581 26.655707417624903 24.05394084518368 70-71 22.515639129195968 26.445940476010982 26.780933503594074 24.257486891198983 72-73 23.074833182605932 26.568529065839027 27.144695308835566 23.21194244271948 74-75 23.262441029130475 26.803699132339283 26.712902599116006 23.22095723941424 76-77 22.97192585432389 26.761964033259872 26.402830157039038 23.8632799553772 78-79 22.779780298463375 26.71076819374052 26.464144505475335 24.04530700232077 80-81 22.362284755823104 27.069876268215275 27.103878659981014 23.46396031598061 82-83 23.210336211805668 26.024317723964334 28.106500125837318 22.658845938392677 84-85 23.37458613176049 26.643420326207817 26.739135813733387 23.2428577282983 86-87 22.04296341414735 27.272834468233537 27.263671888402868 23.420530229216237 88-89 23.218498986522665 25.99029087553385 27.019725210978418 23.77148492696507 90-91 22.78265263594166 26.077367066252105 27.174964063600658 23.965016234205574 92-93 23.048992377799344 26.297167338053967 27.133431859528745 23.52040842461794 94-95 21.913060186113203 26.282193407190725 27.82374239436584 23.981004012330224 96-97 22.295915043703115 26.192061742816485 27.31368837205436 24.198334841426032 98-99 21.61286210205707 26.965028603594266 27.173342062916984 24.248767231431682 100 22.44187716312766 26.16078524622664 26.437681090327775 24.959656500317923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1594.0 1 1151.0 2 836.5 3 952.5 4 859.5 5 744.5 6 670.5 7 661.5 8 750.5 9 763.5 10 651.5 11 591.5 12 602.0 13 619.5 14 634.0 15 897.0 16 1085.0 17 1207.5 18 1590.0 19 1957.0 20 2440.5 21 3152.5 22 4393.0 23 5901.5 24 7218.5 25 9155.0 26 12329.5 27 15869.5 28 20871.5 29 30821.5 30 44632.0 31 57316.5 32 74398.0 33 90580.5 34 101332.5 35 114007.5 36 125435.0 37 147436.0 38 156878.0 39 145627.5 40 135498.5 41 125455.0 42 125346.5 43 135597.0 44 149997.5 45 179771.0 46 207809.5 47 226322.0 48 238979.5 49 234758.0 50 224084.0 51 232378.5 52 238656.0 53 230292.0 54 220419.0 55 193479.5 56 172701.0 57 145258.5 58 116800.5 59 111713.5 60 93389.0 61 65991.5 62 49778.5 63 41481.5 64 30807.5 65 16878.0 66 10482.0 67 7529.5 68 5601.0 69 4174.0 70 3305.0 71 2679.5 72 2086.5 73 1824.5 74 1420.0 75 1006.0 76 719.0 77 456.5 78 272.0 79 160.0 80 97.5 81 62.0 82 46.5 83 34.0 84 25.5 85 22.5 86 27.5 87 27.5 88 22.0 89 17.5 90 12.0 91 12.5 92 11.5 93 7.5 94 8.0 95 9.5 96 6.0 97 5.0 98 7.0 99 7.5 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059918769477224194 2 0.007306224149158305 3 0.00738353869041924 4 0.009374388127888303 5 0.0036531120745791523 6 0.0021261498846756976 7 9.471031304464471E-4 8 0.0012370326601749513 9 4.832158828808403E-4 10-11 3.6724407098943863E-4 12-13 1.8362203549471932E-4 14-15 1.9328635315233616E-4 16-17 5.412017888265411E-4 18-19 0.0017202485430557915 20-21 0.0010727392599954653 22-23 0.0012756899308054184 24-25 0.0012273683425173343 26-27 0.003440497086111583 28-29 0.0038463984277314887 30-31 0.0047258513345746185 32-33 0.005701947417993916 34-35 0.0034115041331387328 36-37 0.0077217898084358284 38-39 0.0058952337711462525 40-41 0.005837247865200551 42-43 0.013636352214897314 44-45 0.01928031372694553 46-47 0.01773402290172684 48-49 0.0207589543285609 50-51 0.018043281066770576 52-53 0.02454736685034669 54-55 0.01830421764352623 56-57 0.014805734651468947 58-59 0.01702852771272081 60-61 0.01582048800551871 62-63 0.010746721235269888 64-65 0.01098832917671031 66-67 0.013385079955799276 68-69 0.00508343108790644 70-71 0.008291984550235219 72-73 0.008205005691316668 74-75 0.010940007588422226 76-77 0.010253841034731431 78-79 0.01174214595400442 80-81 0.009770625151850591 82-83 0.0062818064774509234 84-85 0.010186190811128114 86-87 0.008804193386088911 88-89 0.00759615367888681 90-91 0.012428312507695213 92-93 0.008262991597262369 94-95 0.007112937796005969 96-97 0.009084458598159798 98-99 0.008842850656719378 100 0.26302406936969897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5173671.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.70718788593068 #Duplication Level Percentage of deduplicated Percentage of total 1 73.14350953028502 47.32910813812513 2 15.707894002621286 20.328272970397983 3 5.512527993095836 10.70100553727114 4 2.4417182054004454 6.319868747253763 5 1.224504612425649 3.961712501170759 6 0.6763157739118377 2.6257495111639138 7 0.4036976668610467 1.828549854508272 8 0.253939448564548 1.3145366087932664 9 0.1588558537659453 0.9251204018771655 >10 0.4722602217902215 4.358427740131619 >50 0.0038444856689896566 0.15650118852783126 >100 8.018923032869066E-4 0.07545368431770685 >500 8.687553751488365E-5 0.03634281471311709 >1k 4.3437768368489E-5 0.03935030174831495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.865727063046723E-5 2 0.0 0.0 0.0 0.0 3.865727063046723E-5 3 0.0 0.0 0.0 0.0 3.865727063046723E-5 4 0.0 0.0 0.0 0.0 3.865727063046723E-5 5 0.0 0.0 0.0 0.0 3.865727063046723E-5 6 0.0 0.0 0.0 0.0 3.865727063046723E-5 7 0.0 0.0 0.0 0.0 3.865727063046723E-5 8 0.0 0.0 0.0 0.0 3.865727063046723E-5 9 0.0 0.0 0.0 0.0 3.865727063046723E-5 10-11 0.0 0.0 0.0 0.0 3.865727063046723E-5 12-13 0.0 0.0 0.0 0.0 2.0295067080995291E-4 14-15 0.0 0.0 0.0 0.0 2.4160794144042016E-4 16-17 0.0 0.0 0.0 0.0 2.7060089441327057E-4 18-19 0.0 0.0 0.0 9.664317657616807E-6 3.092581650437378E-4 20-21 0.0 0.0 0.0 3.865727063046723E-5 3.47915435674205E-4 22-23 0.0 0.0 0.0 7.731454126093445E-5 3.47915435674205E-4 24-25 0.0 0.0 0.0 1.1597181189140167E-4 3.47915435674205E-4 26-27 0.0 0.0 0.0 5.122088358536907E-4 3.6724407098943863E-4 28-29 0.0 0.0 0.0 0.0024160794144042017 3.8657270630467226E-4 30-31 0.0 0.0 0.0 0.00986726832842676 3.8657270630467226E-4 32-33 0.0 0.0 0.0 0.0229430901191823 3.8657270630467226E-4 34-35 0.0 0.0 0.0 0.04494874142557577 4.059013416199059E-4 36-37 0.0 0.0 0.0 0.08138321899479113 4.445586122503731E-4 38-39 0.0 0.0 0.0 0.13516514675942864 4.445586122503731E-4 40-41 0.0 0.0 0.0 0.19518055941322904 4.445586122503731E-4 42-43 0.0 0.0 0.0 0.2573124576340475 4.445586122503731E-4 44-45 0.0 0.0 0.0 0.3308289220555385 4.445586122503731E-4 46-47 0.0 0.0 0.0 0.4267086175367548 4.638872475656067E-4 48-49 0.0 0.0 0.0 0.5369784820101626 5.412017888265411E-4 50-51 0.0 0.0 0.0 0.6544096058678645 5.412017888265411E-4 52-53 0.0 0.0 0.0 0.7694343146288196 6.185163300874756E-4 54-55 0.0 0.0 0.0 0.8992067721352981 6.185163300874756E-4 56-57 0.0 0.0 0.0 1.0594798161692154 6.378449654027092E-4 58-59 0.0 0.0 0.0 1.252698519097948 6.765022360331765E-4 60-61 0.0 0.0 0.0 1.4544991361066444 6.765022360331765E-4 62-63 0.0 0.0 0.0 1.6638862424765701 7.151595066636437E-4 64-65 0.0 0.0 0.0 1.8820098920089816 7.151595066636437E-4 66-67 0.0 0.0 0.0 2.132847256812426 7.151595066636437E-4 68-69 0.0 0.0 0.0 2.4207182868798576 7.344881419788773E-4 70-71 0.0 0.0 0.0 2.7391382250630163 7.441524596364941E-4 72-73 0.0 0.0 0.0 3.076094324513484 8.118026832398118E-4 74-75 0.0 0.0 0.0 3.40672996021587 8.891172245007462E-4 76-77 0.0 0.0 0.0 3.7875137402436296 9.277744951312134E-4 78-79 0.0 0.0 0.0 4.197802682080093 9.277744951312134E-4 80-81 0.0 0.0 0.0 4.646922852264862 9.277744951312134E-4 82-83 0.0 0.0 0.0 5.124842689069329 9.277744951312134E-4 84-85 0.0 0.0 0.0 5.586487814938367 9.277744951312134E-4 86-87 0.0 0.0 0.0 6.078314604852144 0.0010244176717073815 88 0.0 0.0 0.0 6.477934139994599 0.0011210608482835496 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5295 0.0 40.47643 94 GTACTAG 5525 0.0 35.99978 1 CAACGCA 10320 0.0 32.69461 5 GGTATCA 7800 0.0 31.950155 1 TAGGCAT 6970 0.0 31.75554 5 GTATCAA 12060 0.0 31.50328 1 ATCAACG 10910 0.0 31.015966 3 TAGTACT 7580 0.0 30.75259 4 GCATCGT 6025 0.0 30.651684 8 CTGTGCG 7615 0.0 30.17573 9 CTAGGCA 7960 0.0 28.87121 4 ACTGTGC 8530 0.0 28.536425 8 CTAGTAC 8800 0.0 27.611212 3 GTACTGT 8605 0.0 27.141167 6 TACTGTG 9190 0.0 26.947218 7 AACGCAG 13210 0.0 26.822023 6 TATCAAC 14290 0.0 26.409544 2 ACGCAGA 12710 0.0 26.139223 7 GTACATG 21850 0.0 25.737803 1 CGCAGAG 12880 0.0 25.6118 8 >>END_MODULE