FastQCFastQC Report
Mon 16 Jan 2017
ERR1146680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173671
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162050.3132205352833607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140320.27121941074335804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90630.17517542186196222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114050.049.1226271
GTGGTAT25450.040.847891
GTATCAA203450.040.4387051
GGGCACC52400.037.42526294
GTACTAG53450.033.0026361
ATCAACG243750.032.631323
CAACGCA244000.032.629215
TATCAAC268250.030.7051732
AACGCAG277600.029.440866
TAGGCAT72200.028.5765865
TCAACGC287800.028.0290934
ACGCAGA292550.027.1973927
CTGTGCG77100.027.192399
CGCAGAG295350.026.8453758
CTAGGCA80650.026.4781234
TAGTACT79550.026.4303574
ACTGTGC87050.025.8077728
GCAGAGT313400.024.9436639
GTACTGT87850.024.6620736
CTATTGA60850.024.6431969
Produced by FastQC (version 0.11.5)