##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5173671
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.186596712469736	33.0	31.0	34.0	30.0	34.0
2	32.24135628260861	33.0	31.0	34.0	30.0	34.0
3	32.394580559915774	34.0	31.0	34.0	30.0	34.0
4	35.98958611786486	37.0	35.0	37.0	35.0	37.0
5	35.90103874019048	37.0	35.0	37.0	35.0	37.0
6	35.946551297908194	37.0	35.0	37.0	35.0	37.0
7	35.869661213478786	37.0	35.0	37.0	35.0	37.0
8	35.88181621908312	37.0	35.0	37.0	35.0	37.0
9	37.59465822237247	39.0	37.0	39.0	35.0	39.0
10-11	37.53716529327049	39.0	37.0	39.0	35.0	39.0
12-13	37.503479927502156	39.0	37.0	39.0	34.5	39.0
14-15	38.730489433904864	40.0	38.0	41.0	34.0	41.0
16-17	38.7331145525102	40.0	38.0	41.0	34.0	41.0
18-19	38.79981023145847	40.0	38.0	41.0	34.5	41.0
20-21	38.79987440252772	40.0	38.0	41.0	34.0	41.0
22-23	38.75981039381901	40.0	38.0	41.0	34.0	41.0
24-25	38.68129274165288	40.0	38.0	41.0	34.0	41.0
26-27	38.53900537935249	40.0	38.0	41.0	34.0	41.0
28-29	38.47934126464555	40.0	38.0	41.0	34.0	41.0
30-31	38.334509094219555	40.0	38.0	41.0	34.0	41.0
32-33	38.181417121421134	40.0	37.0	41.0	33.5	41.0
34-35	38.07079083691251	40.0	37.0	41.0	33.0	41.0
36-37	38.05307710907787	40.0	37.0	41.0	33.0	41.0
38-39	37.950141688561175	40.0	37.0	41.0	33.0	41.0
40-41	37.82697788475533	40.0	37.0	41.0	33.0	41.0
42-43	37.678910970566164	40.0	36.5	41.0	33.0	41.0
44-45	37.45552036455352	40.0	36.0	41.0	32.0	41.0
46-47	37.17844756653447	40.0	36.0	41.0	31.0	41.0
48-49	36.88468903415003	39.5	35.0	41.0	31.0	41.0
50-51	36.66688363059808	39.0	35.0	41.0	30.0	41.0
52-53	36.47866263625963	39.0	35.0	41.0	30.0	41.0
54-55	36.28666869230764	39.0	35.0	41.0	30.0	41.0
56-57	36.05688533345085	38.0	35.0	40.5	30.0	41.0
58-59	35.77008665607071	37.5	35.0	40.0	29.0	41.0
60-61	35.585039133721494	37.0	35.0	40.0	29.0	41.0
62-63	35.366082613293344	36.5	35.0	40.0	29.0	41.0
64-65	35.11205563709018	36.0	34.0	39.5	29.0	41.0
66-67	34.85490389705878	35.5	34.0	39.0	29.0	41.0
68-69	34.595259922789836	35.0	34.0	39.0	29.0	41.0
70-71	34.375277245112805	35.0	34.0	38.0	29.0	41.0
72-73	34.141542629981686	35.0	34.0	37.0	29.0	40.0
74-75	33.888621831577616	35.0	34.0	37.0	28.0	39.5
76-77	33.65329704961912	35.0	34.0	36.0	28.0	39.0
78-79	33.43413158664322	35.0	33.0	36.0	28.0	39.0
80-81	33.308304876749986	35.0	33.0	36.0	28.0	38.0
82-83	33.15746024437966	35.0	33.0	35.0	28.0	37.0
84-85	33.03215328149007	35.0	33.0	35.0	28.0	37.0
86-87	32.897913783075886	35.0	33.0	35.0	27.0	36.5
88-89	32.79882621063457	35.0	33.0	35.0	27.0	36.0
90-91	32.73510588516355	35.0	33.0	35.0	27.0	36.0
92-93	32.67254672745909	35.0	33.0	35.0	27.0	36.0
94-95	32.62855494676798	35.0	33.0	35.0	27.0	35.5
96-97	32.54208414102868	35.0	33.0	35.0	27.0	35.0
98-99	32.467573991465635	35.0	33.0	35.0	27.0	35.0
100	31.25181326760051	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3336170312368125
1101	2	0.43549271179190896
1101	3	0.35734843260399174
1101	4	0.21973613241863177
1101	5	0.2364709469193116
1101	6	0.26054503577468324
1101	7	0.31338203506182793
1101	8	0.2713117706851591
1101	9	0.35807164382668333
1101	10-11	0.33420898700099144
1101	12-13	0.35056154522496286
1101	14-15	0.5149859035154236
1101	16-17	0.5117187943633255
1101	18-19	0.44816929472201394
1101	20-21	0.5012893385019694
1101	22-23	0.46036199471974015
1101	24-25	0.46684667182383066
1101	26-27	0.518761150814413
1101	28-29	0.5430088308157934
1101	30-31	0.5599896781809974
1101	32-33	0.5668452062288907
1101	34-35	0.5774046824066161
1101	36-37	0.5012714480485982
1101	38-39	0.5649583915509098
1101	40-41	0.5316188314168713
1101	42-43	0.4356830411197734
1101	44-45	0.44845461479541626
1101	46-47	0.47302304692809116
1101	48-49	0.43046504442209965
1101	50-51	0.43697913580941616
1101	52-53	0.5244945050851157
1101	54-55	0.5024156057057994
1101	56-57	0.4701838484934058
1101	58-59	0.45816191346869317
1101	60-61	0.5177408054946611
1101	62-63	0.5276932184579266
1101	64-65	0.5062838757623567
1101	66-67	0.5360943034978547
1101	68-69	0.5076803728715404
1101	70-71	0.43903979938313853
1101	72-73	0.488516930485126
1101	74-75	0.438642735550836
1101	76-77	0.43853587810252037
1101	78-79	0.41172833480408855
1101	80-81	0.42144646789664364
1101	82-83	0.4195749003597058
1101	84-85	0.4050437614593747
1101	86-87	0.3929019716094828
1101	88-89	0.4168981419607931
1101	90-91	0.4062385585561046
1101	92-93	0.35865202696447795
1101	94-95	0.35077825083303793
1101	96-97	0.37736019090991135
1101	98-99	0.43155666389090186
1101	100	0.7483044364948341
1102	1	0.270892966055321
1102	2	0.3180229869414717
1102	3	0.2523425517426574
1102	4	0.166756056824795
1102	5	0.17165545189806863
1102	6	0.16291667746071425
1102	7	0.21856708600269315
1102	8	0.26565793363484147
1102	9	0.3462331582097562
1102	10-11	0.30178359086418993
1102	12-13	0.2902710154705659
1102	14-15	0.42265370222527565
1102	16-17	0.39231691920085154
1102	18-19	0.39283770427092435
1102	20-21	0.4128160359314208
1102	22-23	0.4503427695310478
1102	24-25	0.45392226813140724
1102	26-27	0.48282105376878803
1102	28-29	0.47278592118194496
1102	30-31	0.4775410141747898
1102	32-33	0.47665889571540987
1102	34-35	0.5037560815422637
1102	36-37	0.49931727528119296
1102	38-39	0.49865767039575104
1102	40-41	0.4360806854850381
1102	42-43	0.4247975903351744
1102	44-45	0.467008984350862
1102	46-47	0.5152339705024289
1102	48-49	0.5306036560287524
1102	50-51	0.49936870676815914
1102	52-53	0.49161621471881034
1102	54-55	0.4655349730592704
1102	56-57	0.4448170010111312
1102	58-59	0.4389594686839615
1102	60-61	0.4378255264756419
1102	62-63	0.4903388234567103
1102	64-65	0.41309526068278757
1102	66-67	0.42281356168776796
1102	68-69	0.45200863378349965
1102	70-71	0.4405986862113309
1102	72-73	0.41647848494149287
1102	74-75	0.4603078084903487
1102	76-77	0.3978046615209436
1102	78-79	0.38485819772431995
1102	80-81	0.3756813166232078
1102	82-83	0.4072604790008043
1102	84-85	0.38812681827501905
1102	86-87	0.3852927984433876
1102	88-89	0.4201667631236816
1102	90-91	0.4243743842229577
1102	92-93	0.37195959612927965
1102	94-95	0.39123953451781546
1102	96-97	0.3627411583753144
1102	98-99	0.3863587847345187
1102	100	0.6650436110286364
1103	1	0.2976187624584483
1103	2	0.36941007336268683
1103	3	0.2671732591740934
1103	4	0.1845719552291527
1103	5	0.2568160357818883
1103	6	0.23581933080816242
1103	7	0.21940324153858626
1103	8	0.2197900827494479
1103	9	0.30098149244542327
1103	10-11	0.34953745054717444
1103	12-13	0.32803765804116836
1103	14-15	0.41972715784965686
1103	16-17	0.3998284338481852
1103	18-19	0.376147114096149
1103	20-21	0.3903314908018629
1103	22-23	0.39106281333025095
1103	24-25	0.4523206009359342
1103	26-27	0.48275120159109974
1103	28-29	0.46691766835725446
1103	30-31	0.3921258078368126
1103	32-33	0.4309291048882784
1103	34-35	0.41225320006380883
1103	36-37	0.38524172652748234
1103	38-39	0.38732895043234095
1103	40-41	0.40211194296430364
1103	42-43	0.39293056403433724
1103	44-45	0.43436499721048705
1103	46-47	0.5094131586946702
1103	48-49	0.43440003358129786
1103	50-51	0.3969096299740045
1103	52-53	0.3391874356216036
1103	54-55	0.36998182237284283
1103	56-57	0.3495864979597485
1103	58-59	0.38079428503119317
1103	60-61	0.4206359834745328
1103	62-63	0.36330158499891496
1103	64-65	0.39939095767901733
1103	66-67	0.3883181568892269
1103	68-69	0.3683036791508769
1103	70-71	0.3925187075993719
1103	72-73	0.39765518050109705
1103	74-75	0.34362068960948733
1103	76-77	0.3654334450029424
1103	78-79	0.3146889142907341
1103	80-81	0.27469108435710865
1103	82-83	0.2631128065033863
1103	84-85	0.3104119771537128
1103	86-87	0.3166692152032766
1103	88-89	0.27624344583340843
1103	90-91	0.2772340909656137
1103	92-93	0.237585077619066
1103	94-95	0.27590865626218175
1103	96-97	0.300768817888887
1103	98-99	0.3393900464767796
1103	100	0.5184419773280986
1104	1	0.23699169282856758
1104	2	0.28175076373267416
1104	3	0.20687750074479538
1104	4	0.16188645137658142
1104	5	0.16123306968687956
1104	6	0.2065661758874171
1104	7	0.21325453102100056
1104	8	0.22012408656717497
1104	9	0.21168220856228714
1104	10-11	0.2756117579182842
1104	12-13	0.2943235948938465
1104	14-15	0.36442910984369803
1104	16-17	0.31576623064319165
1104	18-19	0.316765073136672
1104	20-21	0.37293701370042953
1104	22-23	0.3879709475391451
1104	24-25	0.38316775963690475
1104	26-27	0.39929116471587633
1104	28-29	0.42920380766747
1104	30-31	0.3714182272504445
1104	32-33	0.3588931693563424
1104	34-35	0.3983335879779091
1104	36-37	0.3454458252020203
1104	38-39	0.3993750122497133
1104	40-41	0.4667710107700245
1104	42-43	0.4856899376225243
1104	44-45	0.41373551956287
1104	46-47	0.4035644619639669
1104	48-49	0.40497740567900564
1104	50-51	0.37674429646525454
1104	52-53	0.37056043006919026
1104	54-55	0.3787664431662847
1104	56-57	0.3778916301050472
1104	58-59	0.3346268505994061
1104	60-61	0.3083939315094071
1104	62-63	0.3463754699018011
1104	64-65	0.3301083850639941
1104	66-67	0.25741276608226116
1104	68-69	0.2795551292018885
1104	70-71	0.28837787997407105
1104	72-73	0.27240043420953697
1104	74-75	0.27481194944244436
1104	76-77	0.23645872884824826
1104	78-79	0.26272755573246087
1104	80-81	0.3011472321047606
1104	82-83	0.29529184781920037
1104	84-85	0.2824408658680113
1104	86-87	0.31086331063474404
1104	88-89	0.32128442899995235
1104	90-91	0.3182954030506764
1104	92-93	0.33274904351689116
1104	94-95	0.27677841420101146
1104	96-97	0.28238582650372734
1104	98-99	0.2673349729129413
1104	100	0.5954785715287123
1105	1	0.1517330915819457
1105	2	0.14402445082671278
1105	3	0.12788665039821012
1105	4	0.168918910160464
1105	5	0.13863311875936546
1105	6	0.09784014100564065
1105	7	0.14386090347915825
1105	8	0.12603528200168057
1105	9	0.1566062115691622
1105	10-11	0.1701925861430027
1105	12-13	0.1463260276304723
1105	14-15	0.2470188268600424
1105	16-17	0.2653129637358802
1105	18-19	0.23904442315675567
1105	20-21	0.26565305261637207
1105	22-23	0.2674994216366784
1105	24-25	0.1872991928045522
1105	26-27	0.22971174091411228
1105	28-29	0.23035743178215284
1105	30-31	0.3169099946378253
1105	32-33	0.28050502350430406
1105	34-35	0.2178751913097301
1105	36-37	0.2740588400402686
1105	38-39	0.28433069551287815
1105	40-41	0.27384797371345115
1105	42-43	0.21302424739428716
1105	44-45	0.23414287605836392
1105	46-47	0.22487054936999584
1105	48-49	0.2737803291005463
1105	50-51	0.2875693424836001
1105	52-53	0.2815867517659001
1105	54-55	0.23270676338935203
1105	56-57	0.22374201676954186
1105	58-59	0.32206024195339467
1105	60-61	0.304312058091341
1105	62-63	0.3869948575108637
1105	64-65	0.3224664948573732
1105	66-67	0.25948872605648887
1105	68-69	0.26790017315541803
1105	70-71	0.3176100915063458
1105	72-73	0.2852767476360327
1105	74-75	0.2875615654587378
1105	76-77	0.23195038148703873
1105	78-79	0.24901215571671997
1105	80-81	0.1596863099365251
1105	82-83	0.22587091343906707
1105	84-85	0.2432655310540781
1105	86-87	0.23634930999301673
1105	88-89	0.2591882546480804
1105	90-91	0.2828085452399094
1105	92-93	0.20902166773964126
1105	94-95	0.23195183607280256
1105	96-97	0.27670720151512285
1105	98-99	0.28043582771011444
1105	100	0.4631617067023157
1106	1	0.12498862621986717
1106	2	0.21695723632911523
1106	3	0.12352164640399366
1106	4	0.1262143534063398
1106	5	0.08543537451186722
1106	6	0.11087998816416444
1106	7	0.0968981809344811
1106	8	0.08191180217664851
1106	9	0.07565497448052838
1106	10-11	0.11657551940334088
1106	12-13	0.08416225374200792
1106	14-15	0.15903582041902098
1106	16-17	0.13047791354286886
1106	18-19	0.17936027319781545
1106	20-21	0.16019257297568146
1106	22-23	0.1341422999277171
1106	24-25	0.08520202171259683
1106	26-27	0.11936079487229279
1106	28-29	0.10795611397131921
1106	30-31	0.15332359674721374
1106	32-33	0.16531388786307843
1106	34-35	0.187797443822177
1106	36-37	0.17096067179174668
1106	38-39	0.19314812927319025
1106	40-41	0.16706144342726503
1106	42-43	0.21164093705793618
1106	44-45	0.22798361870258788
1106	46-47	0.25379507271799184
1106	48-49	0.2807415712727277
1106	50-51	0.30734593192882187
1106	52-53	0.22831219573798478
1106	54-55	0.19535075056318618
1106	56-57	0.1842764731376576
1106	58-59	0.15531583245443414
1106	60-61	0.16627674981075558
1106	62-63	0.21916844934144564
1106	64-65	0.1721500303220722
1106	66-67	0.2431522569404052
1106	68-69	0.24075628653519487
1106	70-71	0.28388029300460005
1106	72-73	0.14635880216960118
1106	74-75	0.1843150108162348
1106	76-77	0.21568758185126313
1106	78-79	0.2356846135260966
1106	80-81	0.18621919375801355
1106	82-83	0.17756398473716217
1106	84-85	0.2321149045492703
1106	86-87	0.20563053328346825
1106	88-89	0.2117754848990785
1106	90-91	0.210334535553244
1106	92-93	0.23054153545579226
1106	94-95	0.1967175741083267
1106	96-97	0.15389214752342184
1106	98-99	0.17022907832879497
1106	100	0.32714550081322713
1107	1	0.1355134673548264
1107	2	0.2004125292523824
1107	3	0.17233506308577518
1107	4	0.11228709069995091
1107	5	0.02184376959332468
1107	6	0.010456433418596589
1107	7	0.09443709172931136
1107	8	0.11313711957378558
1107	9	0.08805620565259886
1107	10-11	0.1739010085990884
1107	12-13	0.08323167799645148
1107	14-15	0.14991516647795322
1107	16-17	0.15340949221526046
1107	18-19	0.13267570700208609
1107	20-21	0.13214558088538553
1107	22-23	0.11115616982177556
1107	24-25	0.08266659657509479
1107	26-27	0.12946382280980373
1107	28-29	0.17864139010401914
1107	30-31	0.1431394459619355
1107	32-33	0.22246579849502268
1107	34-35	0.2174773933787506
1107	36-37	0.09039255830838044
1107	38-39	0.1370519041299474
1107	40-41	0.1760233091580048
1107	42-43	0.16105920876607627
1107	44-45	0.18635229521822083
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2216	62-63	-0.9737481721115202
2216	64-65	-0.9023430577690732
2216	66-67	-0.9317877431413137
2216	68-69	-0.8748651791336428
2216	70-71	-0.8868097979964134
2216	72-73	-0.851372160058375
2216	74-75	-0.7918654132285852
2216	76-77	-0.8915477715171534
2216	78-79	-0.8459908143847841
2216	80-81	-0.847646878161072
2216	82-83	-0.8199767110076408
2216	84-85	-0.8515353504106642
2216	86-87	-0.8916532445365917
2216	88-89	-0.7973318522986474
2216	90-91	-0.7264368332031239
2216	92-93	-0.7966984387916902
2216	94-95	-0.7742212282451817
2216	96-97	-0.8618193520749884
2216	98-99	-0.8388688093925865
2216	100	-0.33488520924709775
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	15.0
13	90.0
14	305.0
15	871.0
16	2080.0
17	4325.0
18	7628.0
19	12158.0
20	17685.0
21	25316.0
22	35900.0
23	42755.0
24	44131.0
25	42408.0
26	42713.0
27	49191.0
28	60720.0
29	75995.0
30	94236.0
31	118346.0
32	149746.0
33	194491.0
34	276480.0
35	412048.0
36	666502.0
37	1270057.0
38	1105131.0
39	421082.0
40	1256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.23560833303857	21.471949028069236	13.567329658186614	24.72511298070558
2	14.44075582397819	20.599667366933243	39.644650151509644	25.314926657578923
3	17.325956752951626	26.839955613721862	29.808756683600485	26.025330949726026
4	11.831159760955405	17.289815454322092	38.58645246289837	32.29257232182414
5	12.543068613459809	38.65202802550184	35.02217387261955	13.782729488418807
6	30.03895881993201	38.752061400151696	17.303294297198814	13.905685482717484
7	26.067902681829857	32.702555961736245	23.52056152026734	17.70897983616656
8	26.80719357686254	34.27616483537511	19.437300902975856	19.47934068478649
9	26.32592988614854	14.230205206322552	21.291148973330543	38.15271593419837
10-11	25.701933887949195	25.851769468912888	27.189262711138763	21.257033931999157
12-13	26.09088402512767	23.720521228735674	27.842622808864785	22.345971937271866
14-15	23.346997485283136	24.629505018898552	25.826523004275735	26.196974491542573
16-17	21.64043674211213	27.064032482931367	27.10614957928326	24.189381195673246
18-19	21.136777840552938	26.76685608099632	30.129268080067774	21.967097998382965
20-21	22.697268535243158	26.049878316576375	29.38027949593239	21.872573652248086
22-23	23.08335802566495	25.62158475094377	29.183842575223668	22.111214648167614
24-25	22.56474174720426	25.69801983929786	29.8571169291592	21.880121484338684
26-27	22.434776003344627	25.946141530839512	28.872777182777952	22.746305283037906
28-29	21.615348173472956	26.462554344874267	29.456598612474583	22.46549886917819
30-31	22.469451575100155	26.037459668386337	29.357239762636627	22.13584899387688
32-33	22.929985304438567	26.310940529461575	28.539686810390535	22.219387355709323
34-35	22.75063489734852	26.160389788991225	29.323636930141095	21.76533838351917
36-37	22.563002170025886	25.690124091771587	28.96158259773379	22.78529114046873
38-39	22.64418243834987	25.761910643332364	29.591280543351132	22.00262637496663
40-41	22.175337395825903	25.14137447085445	30.02170992318607	22.66157821013358
42-43	22.444189048743148	25.325528043820334	29.125170502724274	23.105112404712244
44-45	22.66404261113627	26.239095991994855	28.75295897245882	22.343902424410057
46-47	22.740236091548923	26.14904388006118	27.974188926972744	23.136531101417155
48-49	23.65403598334722	26.012448414288425	27.784845615424715	22.54866998693964
50-51	22.97563954105315	26.02446116113684	27.573119744181646	23.426779553628364
52-53	22.38815533496428	26.605305980994924	27.4131269653598	23.59341171868099
54-55	22.467827969733676	26.62896423062077	27.208340074194897	23.694867725450653
56-57	23.5615774563168	25.983397475409625	27.000344629567667	23.454680438705903
58-59	22.422166235750808	26.397255101576338	27.854035157438034	23.326543505234824
60-61	22.562200031660304	26.967891850873393	27.111580925806837	23.358327191659463
62-63	23.003231167965648	26.26741244273167	26.729937021507556	23.99941936779513
64-65	22.186434176664324	26.446717084128185	27.15740208039921	24.209446658808286
66-67	22.90693650238182	26.053708718952024	27.12746366624134	23.911891112424815
68-69	22.475395130459592	25.87395874225478	27.539004702850256	24.111641424435376
70-71	22.40463278656553	25.62793959734203	27.55978785949137	24.407639756601075
72-73	22.97913621591239	25.63563392526829	27.87237028220287	23.51285957661645
74-75	23.08002905104276	26.117834547256507	27.35087058900844	23.45126581269229
76-77	22.779325681117736	26.12830556288434	26.89560495316183	24.196763802836095
78-79	22.54568589167014	26.114641433001967	27.0718535322263	24.267819143101597
80-81	22.168939617536562	26.552867393384698	27.615256169168855	23.662936819909884
82-83	22.96655508245499	25.550349065489474	28.594812078309577	22.888283773745954
84-85	23.233667158193864	26.131715758501073	27.08460781522443	23.550009268080636
86-87	21.83978262243579	26.713507681489602	27.63527097103778	23.811438725036826
88-89	22.828857884469265	25.549672563253445	27.426840632115955	24.194628920161332
90-91	22.378983897507204	25.55755864646206	27.63660464687453	24.42685280915621
92-93	22.71173601877661	25.866072659046157	27.3175855209966	24.104605801180632
94-95	21.524812845656403	25.842839639397248	28.040979026304534	24.59136848864182
96-97	21.921600735725175	25.66650449941637	27.54236788539511	24.869526879463344
98-99	21.272361539804134	26.42751152904775	27.431315211191436	24.86881171995668
100	22.03806887459847	25.731382567200388	26.5527615136567	25.677787044544438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1227.0
1	810.0
2	538.0
3	696.0
4	476.0
5	331.0
6	755.0
7	1829.5
8	3024.0
9	3312.5
10	2440.5
11	1550.5
12	1371.0
13	1472.5
14	1726.0
15	2367.0
16	2934.5
17	3525.0
18	4697.5
19	6080.5
20	7665.0
21	9324.5
22	11094.0
23	13204.0
24	14891.0
25	16609.5
26	19219.5
27	21803.5
28	26085.0
29	35416.0
30	47905.5
31	58981.5
32	74890.5
33	89434.0
34	98292.5
35	109798.0
36	120736.0
37	141963.5
38	151518.0
39	141511.0
40	131949.0
41	122784.0
42	122112.0
43	131516.5
44	145833.0
45	176091.0
46	204096.5
47	222673.5
48	236667.5
49	231617.5
50	221634.5
51	228471.0
52	233684.0
53	226532.0
54	220722.5
55	194430.5
56	170351.5
57	144577.0
58	116528.0
59	112549.5
60	93933.5
61	64855.0
62	48181.0
63	39474.0
64	28942.5
65	15526.5
66	9553.5
67	6707.5
68	4883.5
69	3654.0
70	3031.5
71	2464.5
72	1858.0
73	1648.5
74	1247.5
75	856.0
76	557.5
77	298.0
78	154.0
79	74.5
80	34.0
81	9.0
82	3.5
83	4.0
84	2.5
85	0.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0077894400320391455
3	0.0
4	0.09202363273582723
5	0.004638872475656067
6	5.798590594570084E-5
7	3.865727063046723E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.2227930612518654E-4
14-15	0.010128204905182413
16-17	0.0
18-19	7.731454126093445E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	9.664317657616807E-6
60-61	0.0
62-63	0.0
64-65	9.664317657616807E-6
66-67	8.697885891855125E-5
68-69	0.0
70-71	4.7355156522322354E-4
72-73	3.2858680035897144E-4
74-75	3.5757975333182185E-4
76-77	1.1597181189140167E-4
78-79	9.664317657616807E-6
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.066935064096654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5173671.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.91071328297855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0369698051118	43.269125002907685
2	16.700560966564755	20.344861613986577
3	6.091538198487463	11.131198099811446
4	2.6564904406626453	6.472349102807029
5	1.3167696241117677	4.010268851700365
6	0.7261734821867266	2.6539046860306703
7	0.4335703909443267	1.848635723955916
8	0.2889552847237582	1.4080377999528213
9	0.17229774952955967	0.9445300938807714
>10	0.5630740649733446	4.947141353626491
>50	0.007131607886924292	0.29364252784990674
>100	0.004947402565031441	0.6223290169134127
>500	6.913555825933394E-4	0.2953662825140657
>1k	6.913555577718212E-4	0.6553026879747491
>5k	4.609037051812124E-5	0.2544344212627511
>10k+	9.218074103624248E-5	0.8488727348253122
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16205	0.3132205352833607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14032	0.27121941074335804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9063	0.17517542186196222	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9328635315233614E-5
2	0.0	0.0	0.0	0.0	1.9328635315233614E-5
3	0.0	0.0	0.0	0.0	1.9328635315233614E-5
4	0.0	0.0	0.0	0.0	1.9328635315233614E-5
5	0.0	0.0	0.0	0.0	1.9328635315233614E-5
6	0.0	0.0	0.0	0.0	3.865727063046723E-5
7	0.0	0.0	0.0	0.0	3.865727063046723E-5
8	0.0	0.0	0.0	0.0	3.865727063046723E-5
9	0.0	0.0	0.0	0.0	3.865727063046723E-5
10-11	0.0	0.0	0.0	3.865727063046723E-5	3.865727063046723E-5
12-13	0.0	0.0	0.0	5.798590594570084E-5	1.3530044720663528E-4
14-15	0.0	0.0	0.0	5.798590594570084E-5	1.6429340017948572E-4
16-17	0.0	0.0	0.0	5.798590594570084E-5	1.8362203549471932E-4
18-19	0.0	0.0	0.0	5.798590594570084E-5	2.0295067080995291E-4
20-21	0.0	0.0	0.0	7.731454126093445E-5	2.1261498846756973E-4
22-23	0.0	0.0	0.0	1.1597181189140167E-4	2.7060089441327057E-4
24-25	0.0	0.0	0.0	1.739577178371025E-4	2.7060089441327057E-4
26-27	0.0	0.0	0.0	5.701947417993915E-4	2.9959384738612103E-4
28-29	0.0	0.0	0.0	0.0024547366850346686	3.092581650437378E-4
30-31	0.0	0.0	0.0	0.009886596963741993	3.092581650437378E-4
32-33	1.9328635315233614E-5	0.0	0.0	0.023146040789992252	3.47915435674205E-4
34-35	1.9328635315233614E-5	0.0	0.0	0.045325649814222824	3.5757975333182185E-4
36-37	1.9328635315233614E-5	0.0	0.0	0.0818277776070415	3.6724407098943863E-4
38-39	1.9328635315233614E-5	0.0	0.0	0.1355903767363638	3.8657270630467226E-4
40-41	1.9328635315233614E-5	0.0	0.0	0.1952772025898052	3.8657270630467226E-4
42-43	1.9328635315233614E-5	0.0	0.0	0.2574670867165693	3.962370239622891E-4
44-45	1.9328635315233614E-5	0.0	0.0	0.33131213793841935	4.735515652232235E-4
46-47	1.9328635315233614E-5	0.0	0.0	0.42735612681981516	5.605304241417747E-4
48-49	1.9328635315233614E-5	0.0	0.0	0.5376163269755654	5.99187694772242E-4
50-51	1.9328635315233614E-5	0.0	0.0	0.6555499953514632	6.185163300874756E-4
52-53	1.9328635315233614E-5	0.0	0.0	0.7711062415835874	6.378449654027092E-4
54-55	1.9328635315233614E-5	0.0	0.0	0.9012652717963705	6.378449654027092E-4
56-57	1.9328635315233614E-5	0.0	0.0	1.062591726454968	6.378449654027092E-4
58-59	1.9328635315233614E-5	0.0	0.0	1.257269741350001	6.378449654027092E-4
60-61	1.9328635315233614E-5	0.0	0.0	1.4607229566781499	6.378449654027092E-4
62-63	1.9328635315233614E-5	0.0	0.0	1.671685346826267	6.765022360331765E-4
64-65	1.9328635315233614E-5	0.0	0.0	1.8913649515015547	6.765022360331765E-4
66-67	1.9328635315233614E-5	0.0	0.0	2.143680956906614	6.765022360331765E-4
68-69	1.9328635315233614E-5	0.0	0.0	2.433349550058363	6.765022360331765E-4
70-71	1.9328635315233614E-5	0.0	0.0	2.7535863799611535	6.765022360331765E-4
72-73	1.9328635315233614E-5	0.0	0.0	3.0923980284018833	7.054951890060269E-4
74-75	1.9328635315233614E-5	0.0	0.0	3.4258943021309243	7.344881419788773E-4
76-77	1.9328635315233614E-5	0.0	0.0	3.8095193915500234	7.441524596364941E-4
78-79	1.9328635315233614E-5	0.0	0.0	4.223519431367012	7.53816777294111E-4
80-81	1.9328635315233614E-5	0.0	0.0	4.676949887227077	7.731454126093445E-4
82-83	1.9328635315233614E-5	0.0	0.0	5.159276652883417	8.118026832398118E-4
84-85	1.9328635315233614E-5	0.0	0.0	5.62540602214559	8.118026832398118E-4
86-87	1.9328635315233614E-5	0.0	0.0	6.122847780618443	8.407956362126622E-4
88	1.9328635315233614E-5	0.0	0.0	6.527492760942859	8.697885891855126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11405	0.0	49.122627	1
GTGGTAT	2545	0.0	40.84789	1
GTATCAA	20345	0.0	40.438705	1
GGGCACC	5240	0.0	37.425262	94
GTACTAG	5345	0.0	33.002636	1
ATCAACG	24375	0.0	32.63132	3
CAACGCA	24400	0.0	32.62921	5
TATCAAC	26825	0.0	30.705173	2
AACGCAG	27760	0.0	29.44086	6
TAGGCAT	7220	0.0	28.576586	5
TCAACGC	28780	0.0	28.029093	4
ACGCAGA	29255	0.0	27.197392	7
CTGTGCG	7710	0.0	27.19239	9
CGCAGAG	29535	0.0	26.845375	8
CTAGGCA	8065	0.0	26.478123	4
TAGTACT	7955	0.0	26.430357	4
ACTGTGC	8705	0.0	25.807772	8
GCAGAGT	31340	0.0	24.943663	9
GTACTGT	8785	0.0	24.662073	6
CTATTGA	6085	0.0	24.643196	9
>>END_MODULE