FastQCFastQC Report
Mon 16 Jan 2017
ERR1146679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4343788
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC63090.1452418948622723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC44750.040.74376794
GTACTAG47900.036.3201981
GGTATCA63650.034.0552861
GTATCAA99850.032.9633981
CAACGCA91200.032.6705445
TAGGCAT59300.031.3043275
ATCAACG96200.030.9739193
GCATCGT51550.030.5378258
TATTCTC23250.030.117945
TAGTACT66650.029.6868344
CTGTGCG67800.029.1102929
CAGTACG7700.028.6836289
AGGGCTA56500.028.2808465
CTAGGCA68350.028.2608534
ACTGTGC74150.027.4409228
AACGCAG116150.027.431056
GCTATTG57450.027.401658
TATCAAC120100.027.3145712
CTATTGA57900.027.1889979
CTAGTAC79300.026.7291583
Produced by FastQC (version 0.11.5)