##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146679_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4343788
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313193921986986	31.0	31.0	34.0	28.0	34.0
2	31.34457897116526	31.0	31.0	34.0	28.0	34.0
3	31.49753924454877	33.0	31.0	34.0	28.0	34.0
4	34.918783329204835	37.0	35.0	37.0	32.0	37.0
5	35.0897454019395	37.0	35.0	37.0	32.0	37.0
6	35.14075479742566	37.0	35.0	37.0	32.0	37.0
7	35.07530869370236	37.0	35.0	37.0	32.0	37.0
8	35.070120134776374	37.0	35.0	37.0	32.0	37.0
9	36.52756994586292	39.0	37.0	39.0	32.0	39.0
10-11	36.348541526428086	39.0	37.0	39.0	31.0	39.0
12-13	36.26638592859504	39.0	37.0	39.0	31.0	39.0
14-15	37.41898384543629	40.0	37.0	41.0	31.0	41.0
16-17	37.45251183989642	40.0	37.0	41.0	31.0	41.0
18-19	37.42437856083215	40.0	37.0	41.0	31.0	41.0
20-21	37.505820841164436	40.0	37.0	41.0	31.0	41.0
22-23	37.45640671229812	40.0	37.0	41.0	31.0	41.0
24-25	37.42091568465128	40.0	37.0	41.0	31.0	41.0
26-27	37.45985025051867	40.0	37.0	41.0	31.0	41.0
28-29	37.46190859222411	40.0	37.0	41.0	31.0	41.0
30-31	37.33797609367676	40.0	36.0	41.0	30.0	41.0
32-33	37.33561835890702	40.0	36.0	41.0	30.0	41.0
34-35	37.273113006435864	40.0	36.0	41.0	30.0	41.0
36-37	37.153906452156505	40.0	36.0	41.0	30.0	41.0
38-39	37.04552742905501	40.0	36.0	41.0	30.0	41.0
40-41	36.95346089634209	40.0	35.5	41.0	30.0	41.0
42-43	36.7809743477352	39.5	35.0	41.0	30.0	41.0
44-45	36.60958096942116	39.0	35.0	41.0	29.5	41.0
46-47	36.59707529465066	39.0	35.0	41.0	29.5	41.0
48-49	36.45854252095176	39.0	35.0	41.0	29.0	41.0
50-51	36.27419363007587	39.0	35.0	41.0	29.0	41.0
52-53	36.05708968761827	38.5	35.0	40.0	28.0	41.0
54-55	35.84654499713153	38.0	34.0	40.0	28.0	41.0
56-57	35.60248566458584	37.5	34.0	40.0	28.0	41.0
58-59	35.3081791744901	37.0	34.0	40.0	28.0	41.0
60-61	35.053917686590594	36.5	33.5	40.0	27.0	41.0
62-63	34.74007536739823	36.0	33.0	39.5	27.0	41.0
64-65	34.5890399117084	35.5	33.0	39.0	27.0	41.0
66-67	34.336952678169375	35.0	33.0	39.0	26.5	41.0
68-69	34.21452761967205	35.0	33.0	39.0	26.0	41.0
70-71	34.12873821190169	35.0	33.0	38.0	27.0	41.0
72-73	33.91504580794459	35.0	33.0	37.0	26.0	40.0
74-75	33.66767795757988	35.0	33.0	37.0	26.0	39.5
76-77	33.41230902613111	35.0	33.0	36.5	26.0	39.0
78-79	33.17193081246138	35.0	33.0	36.0	26.0	39.0
80-81	32.986700778214775	35.0	33.0	36.0	26.0	38.5
82-83	32.73755959084559	35.0	33.0	35.5	25.5	37.0
84-85	32.558250770986064	35.0	32.5	35.0	25.0	37.0
86-87	32.38901000693404	35.0	32.0	35.0	25.0	36.5
88-89	32.26733429439926	35.0	32.0	35.0	25.0	36.0
90-91	32.1220717954007	35.0	32.0	35.0	25.0	36.0
92-93	31.97940829524829	35.0	32.0	35.0	25.0	36.0
94-95	31.913691344973557	35.0	32.0	35.0	25.0	35.5
96-97	31.807339930033418	35.0	32.0	35.0	24.0	35.0
98-99	31.718635785171834	35.0	32.0	35.0	24.0	35.0
100	30.7712694542183	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.466824015678597
1101	2	0.5317166388443013
1101	3	0.47077414305721277
1101	4	0.6670791964544733
1101	5	0.504207414858115
1101	6	0.5060231903047736
1101	7	0.5521262695570286
1101	8	0.5392714004153376
1101	9	0.8221722463511938
1101	10-11	0.7892027789883969
1101	12-13	0.6980580143826103
1101	14-15	0.8771023920523433
1101	16-17	0.886467402597809
1101	18-19	0.8452465504318525
1101	20-21	0.837010601447318
1101	22-23	0.8174188742866164
1101	24-25	0.8597644925794086
1101	26-27	0.8219075039999595
1101	28-29	0.7905398118724065
1101	30-31	0.8580668754518683
1101	32-33	0.8467104023133629
1101	34-35	0.8394029296454661
1101	36-37	0.8619433165708017
1101	38-39	0.7676585823045272
1101	40-41	0.7611478384200581
1101	42-43	0.7660940465557289
1101	44-45	0.8008103507666675
1101	46-47	0.8242102825381181
1101	48-49	0.7247545147021839
1101	50-51	0.6449725050633575
1101	52-53	0.7032262333080865
1101	54-55	0.6971042296666496
1101	56-57	0.7143795313811836
1101	58-59	0.6451994133818886
1101	60-61	0.7019394503289433
1101	62-63	0.6674931361057475
1101	64-65	0.6582758363866077
1101	66-67	0.7240591159036001
1101	68-69	0.7300722468608996
1101	70-71	0.8051978810262383
1101	72-73	0.7790258814000239
1101	74-75	0.7004984957322264
1101	76-77	0.677916860901604
1101	78-79	0.6278924256500176
1101	80-81	0.6311805828801837
1101	82-83	0.5664072556804172
1101	84-85	0.5504046357798984
1101	86-87	0.5659590071602523
1101	88-89	0.6568744935871607
1101	90-91	0.6184044661257104
1101	92-93	0.5807465279563822
1101	94-95	0.5778505435624126
1101	96-97	0.5600015249338561
1101	98-99	0.5974964130902194
1101	100	0.7413276766091741
1102	1	0.3058211687850587
1102	2	0.3792360892591198
1102	3	0.38035952405114415
1102	4	0.5045261083745345
1102	5	0.36193954496656033
1102	6	0.3798985036708231
1102	7	0.38071406682195175
1102	8	0.38290479708292224
1102	9	0.5128125015013509
1102	10-11	0.6423228408368757
1102	12-13	0.5416500486208804
1102	14-15	0.7003150251581403
1102	16-17	0.6924810087594935
1102	18-19	0.7193212470758894
1102	20-21	0.6712787760483963
1102	22-23	0.688294435701323
1102	24-25	0.7664093007446127
1102	26-27	0.6559109464621145
1102	28-29	0.648389481791412
1102	30-31	0.6498188796724662
1102	32-33	0.6303671432561231
1102	34-35	0.7220250432467168
1102	36-37	0.7010901173096613
1102	38-39	0.6802371048158875
1102	40-41	0.659363461960254
1102	42-43	0.6982822009099934
1102	44-45	0.619377170921311
1102	46-47	0.586743476359814
1102	48-49	0.617398360891869
1102	50-51	0.583495973362858
1102	52-53	0.5382411351148093
1102	54-55	0.5493022933761296
1102	56-57	0.5776037358941579
1102	58-59	0.6042006838443257
1102	60-61	0.593141970100433
1102	62-63	0.6142569824465198
1102	64-65	0.6347318631506766
1102	66-67	0.669843862547701
1102	68-69	0.6596238453897243
1102	70-71	0.6083238607869745
1102	72-73	0.5693352371156593
1102	74-75	0.5353472553000316
1102	76-77	0.5895195574549845
1102	78-79	0.5852435429392671
1102	80-81	0.5182581918925351
1102	82-83	0.5580216999192089
1102	84-85	0.5466370032343946
1102	86-87	0.4621830013984791
1102	88-89	0.537327251649053
1102	90-91	0.47870182232988157
1102	92-93	0.545310572987912
1102	94-95	0.5064968274283004
1102	96-97	0.5139002958968
1102	98-99	0.4826432087925525
1102	100	0.5257171054550938
1103	1	0.25517237899236633
1103	2	0.31101651718565293
1103	3	0.32221482228559495
1103	4	0.4125643579620686
1103	5	0.2952197213409633
1103	6	0.3053769528995005
1103	7	0.32950717775814553
1103	8	0.3252715927869687
1103	9	0.3800323063398423
1103	10-11	0.5270983494674795
1103	12-13	0.5186078488597019
1103	14-15	0.5967292971184506
1103	16-17	0.528975928675159
1103	18-19	0.5702263223780619
1103	20-21	0.5087989355973477
1103	22-23	0.5936292440264026
1103	24-25	0.6098914654300103
1103	26-27	0.5310570345702601
1103	28-29	0.5156174358454493
1103	30-31	0.5796993936293404
1103	32-33	0.5285932737401993
1103	34-35	0.5587113233347694
1103	36-37	0.5268684771897512
1103	38-39	0.5961602572252502
1103	40-41	0.5786707103215107
1103	42-43	0.6089691895406162
1103	44-45	0.5615796403776159
1103	46-47	0.5359587361807172
1103	48-49	0.5103380659683339
1103	50-51	0.5017264029241133
1103	52-53	0.5260096993450247
1103	54-55	0.5217914932256349
1103	56-57	0.4621224466394622
1103	58-59	0.5397411207886762
1103	60-61	0.5895612194259243
1103	62-63	0.5496613714090728
1103	64-65	0.5261705510391437
1103	66-67	0.49317228520602896
1103	68-69	0.42434611085212737
1103	70-71	0.4790956256581964
1103	72-73	0.43045542730957465
1103	74-75	0.4484756903529856
1103	76-77	0.38213068323251775
1103	78-79	0.3899069681375167
1103	80-81	0.3732707890182567
1103	82-83	0.37894478645168306
1103	84-85	0.4249425714909094
1103	86-87	0.37505573651431945
1103	88-89	0.468378544373266
1103	90-91	0.4580409746260372
1103	92-93	0.38433292086963533
1103	94-95	0.3405655258033775
1103	96-97	0.4094062963057219
1103	98-99	0.3424159969515017
1103	100	0.437804978397601
1104	1	0.22710615978451187
1104	2	0.2972914824846207
1104	3	0.2857589080116121
1104	4	0.3793237563995788
1104	5	0.30127608166674946
1104	6	0.2822286517860064
1104	7	0.3012141385698186
1104	8	0.35276236849141185
1104	9	0.37921230965747554
1104	10-11	0.5532754129555784
1104	12-13	0.42105885829109013
1104	14-15	0.5401591810462989
1104	16-17	0.5786461324235361
1104	18-19	0.5483558668134023
1104	20-21	0.4658843648322062
1104	22-23	0.47823260186454775
1104	24-25	0.5145157498978818
1104	26-27	0.4880730835757987
1104	28-29	0.4926963792418064
1104	30-31	0.4063163568819306
1104	32-33	0.4807719681973808
1104	34-35	0.4744480038226513
1104	36-37	0.4595922033214421
1104	38-39	0.42460689356742165
1104	40-41	0.5029741510132268
1104	42-43	0.5536654567241754
1104	44-45	0.4975193705972032
1104	46-47	0.4245609909217265
1104	48-49	0.40708843430216035
1104	50-51	0.3134079618386707
1104	52-53	0.39877402455510236
1104	54-55	0.447820500274716
1104	56-57	0.39989348741907804
1104	58-59	0.3667522049838112
1104	60-61	0.4299616811392255
1104	62-63	0.4020158283052382
1104	64-65	0.3857906705099481
1104	66-67	0.3464629142804512
1104	68-69	0.3619372846001667
1104	70-71	0.348397319472177
1104	72-73	0.32596533293838803
1104	74-75	0.26286490975214605
1104	76-77	0.299608989417564
1104	78-79	0.35374862469674184
1104	80-81	0.31846884992332747
1104	82-83	0.2914602957227004
1104	84-85	0.36789325878941526
1104	86-87	0.3179821528619726
1104	88-89	0.25341368012247756
1104	90-91	0.3491251008710776
1104	92-93	0.3317788041694705
1104	94-95	0.32590600491219135
1104	96-97	0.3522890952924236
1104	98-99	0.364784837873259
1104	100	0.43803170134279057
1105	1	0.21771475743183188
1105	2	0.22693543271672922
1105	3	0.2558590440319435
1105	4	0.3270110884188995
1105	5	0.23266543851843124
1105	6	0.21376801974178505
1105	7	0.23880805187167198
1105	8	0.24599465938709386
1105	9	0.4327282658738625
1105	10-11	0.37966702815275255
1105	12-13	0.2476403688788551
1105	14-15	0.4492820644848834
1105	16-17	0.3367036002438013
1105	18-19	0.3415480232437176
1105	20-21	0.3418166540151262
1105	22-23	0.36091570345526236
1105	24-25	0.3708355906994427
1105	26-27	0.4066573472858934
1105	28-29	0.3583178466679442
1105	30-31	0.37897977997944565
1105	32-33	0.296476795706738
1105	34-35	0.3809663928361857
1105	36-37	0.40971081697635725
1105	38-39	0.40243119419236706
1105	40-41	0.36510632070686455
1105	42-43	0.4220973470642235
1105	44-45	0.4160019149433367
1105	46-47	0.427268347354655
1105	48-49	0.3407297618898397
1105	50-51	0.3959738353805662
1105	52-53	0.4085487538711732
1105	54-55	0.3665843394966828
1105	56-57	0.2935788426403718
1105	58-59	0.4091389297612622
1105	60-61	0.4063885402858247
1105	62-63	0.3434455370854863
1105	64-65	0.3485710296205582
1105	66-67	0.2574121636668423
1105	68-69	0.2510368970730852
1105	70-71	0.30505000402624916
1105	72-73	0.30545404477119575
1105	74-75	0.3339026007396626
1105	76-77	0.3447022647632778
1105	78-79	0.3641315532983853
1105	80-81	0.2854619687532889
1105	82-83	0.3214626488474863
1105	84-85	0.27818495870100435
1105	86-87	0.2666910446234212
1105	88-89	0.27543020318647393
1105	90-91	0.3146200090573501
1105	92-93	0.2786878032384159
1105	94-95	0.31058976533935834
1105	96-97	0.21756922491202246
1105	98-99	0.31459968866465005
1105	100	0.420007828696928
1106	1	0.09174771009381644
1106	2	0.1555900932262304
1106	3	0.14305461872751835
1106	4	0.25732080703506455
1106	5	0.17843533993350036
1106	6	0.19883058141724774
1106	7	0.21624141841794398
1106	8	0.10064474992427819
1106	9	0.30545884828186587
1106	10-11	0.32079689871810757
1106	12-13	0.2779649375098785
1106	14-15	0.3918059203151856
1106	16-17	0.30681016107616443
1106	18-19	0.28036775843441575
1106	20-21	0.3151228541358435
1106	22-23	0.33985472977331455
1106	24-25	0.36489378838666653
1106	26-27	0.385384102222325
1106	28-29	0.3553801784076924
1106	30-31	0.3449986945870762
1106	32-33	0.2891736422342319
1106	34-35	0.28313144117058897
1106	36-37	0.30911753493145966
1106	38-39	0.34845348598078374
1106	40-41	0.33867389306475815
1106	42-43	0.29352496042731957
1106	44-45	0.30190799338973306
1106	46-47	0.261310150146457
1106	48-49	0.1688546121921135
1106	50-51	0.22644983823560239
1106	52-53	0.19635042473736064
1106	54-55	0.25437092083164714
1106	56-57	0.19149876273770872
1106	58-59	0.12259651620271939
1106	60-61	0.21561824366875015
1106	62-63	0.21300851551942657
1106	64-65	0.18089400307256653
1106	66-67	0.1682357287242766
1106	68-69	0.20213693587473358
1106	70-71	0.21556369478187776
1106	72-73	0.19755760925378496
1106	74-75	0.1967297800990977
1106	76-77	0.15252451008552015
1106	78-79	0.146278841494059
1106	80-81	0.13288195831037086
1106	82-83	0.21195845970620297
1106	84-85	0.18761004756157718
1106	86-87	0.22463586244727907
1106	88-89	0.20697013775060213
1106	90-91	0.2464677221550602
1106	92-93	0.23400153375376576
1106	94-95	0.24922567171720544
1106	96-97	0.23321293712831803
1106	98-99	0.2445097143408077
1106	100	0.3733530340582618
1107	1	0.11739878072396692
1107	2	0.1550809054785489
1107	3	0.1335459491770088
1107	4	0.3498168273553546
1107	5	0.14297518208839932
1107	6	0.26349462934489765
1107	7	0.2126547359609674
1107	8	0.2073116609844945
1107	9	0.24941060499512702
1107	10-11	0.2886188116236994
1107	12-13	0.2754980642271079
1107	14-15	0.3618068709646778
1107	16-17	0.24800736430265857
1107	18-19	0.1822083836013988
1107	20-21	0.21973869232908072
1107	22-23	0.2999535476127164
1107	24-25	0.3959161839488132
1107	26-27	0.2732907223465162
1107	28-29	0.29031583248560366
1107	30-31	0.4230182804518492
1107	32-33	0.32160554652806184
1107	34-35	0.3684991436931071
1107	36-37	0.39352542320964545
1107	38-39	0.37069433458146506
1107	40-41	0.35487438939077975
1107	42-43	0.35528875074759725
1107	44-45	0.3728905983681585
1107	46-47	0.3735259339103578
1107	48-49	0.34152114050717586
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2216	56-57	-1.0754002005898258
2216	58-59	-1.1198264405059533
2216	60-61	-1.2083868053362963
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2216	64-65	-1.1255537319465958
2216	66-67	-1.118998915653222
2216	68-69	-1.1834030389509564
2216	70-71	-1.0403621682868405
2216	72-73	-1.018798267816372
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2216	76-77	-1.0446554013800977
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2216	80-81	-0.9883807879523729
2216	82-83	-0.9934897278848425
2216	84-85	-1.024195360672401
2216	86-87	-0.9876698498584346
2216	88-89	-1.0687787272839806
2216	90-91	-1.190727322182834
2216	92-93	-1.1059418240467416
2216	94-95	-1.1001350705006843
2216	96-97	-1.0896193142753638
2216	98-99	-1.0922179081011016
2216	100	-0.7199828449919039
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15.0
3	22.0
4	30.0
5	22.0
6	41.0
7	199.0
8	514.0
9	903.0
10	1331.0
11	2497.0
12	4334.0
13	6296.0
14	8630.0
15	11222.0
16	13647.0
17	15801.0
18	18066.0
19	20287.0
20	23349.0
21	26629.0
22	30191.0
23	35024.0
24	40364.0
25	45954.0
26	51791.0
27	56875.0
28	65170.0
29	79138.0
30	92910.0
31	106999.0
32	131525.0
33	171165.0
34	248957.0
35	360264.0
36	563966.0
37	970712.0
38	819216.0
39	319278.0
40	454.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99161491734813	21.435769764730733	12.937586855124097	23.63502846279704
2	15.344992796102296	19.74436638623114	41.1903629392271	23.720277878439465
3	18.157001443083836	27.088442528518637	29.23966887350132	25.514887154896215
4	11.986657445003864	17.104427042802453	40.586237128735206	30.322678383458474
5	13.013886749744511	38.7735208996516	35.095888537647134	13.116703812956745
6	29.26335530247708	38.97650557086775	18.35089606295877	13.409243063696398
7	26.56184927982651	32.11696358787176	24.013932677908116	17.307254454393618
8	26.02750227742947	35.08558231172271	19.985344335594917	18.9015710752529
9	26.092637842750765	15.3271801303665	21.729867759546238	36.8503142673365
10-11	25.50276402082296	26.05521135190978	27.62774207844006	20.814282548827197
12-13	25.753748440152325	24.060911555356192	27.48909444748903	22.696245557002452
14-15	23.2553197954959	25.21003615287713	25.058117637741912	26.47652641388506
16-17	22.040485004166325	27.692747049702255	26.3159203330113	23.950847613120118
18-19	21.49466270002285	28.412484913573554	27.988252011955183	22.104600374448413
20-21	23.367757838175322	27.519026446348917	26.802699460708723	22.310516254767045
22-23	23.94180143884892	27.092305035971222	26.509214388489212	22.456679136690646
24-25	23.311585962661006	27.212460864689643	27.292253789847397	22.183699382801954
26-27	23.03703372014708	27.55794407444708	26.31798445063305	23.0870377547728
28-29	22.045999806152786	28.021950319398975	26.958806545773843	22.9732433286744
30-31	22.933026520719636	27.579741158513073	26.857031691563538	22.630200629203756
32-33	23.495085638008316	27.80198291970993	25.84241971044616	22.8605117318356
34-35	23.360314132521665	27.620665088959285	26.925902890993957	22.093117887525096
36-37	22.939685722722047	27.26607777329466	26.621303420143978	23.172933083839315
38-39	23.156309923884507	27.2394740592389	27.205653649070307	22.398562367806285
40-41	22.70397741380674	26.351855750789362	27.98086168684818	22.963305148555722
42-43	22.704069239350293	26.667971389725764	27.131362971268846	23.496596399655093
44-45	22.89969327293505	27.704389344659585	26.88151530215989	22.514402080245475
46-47	22.860715427532256	27.386860476883378	26.43807183103245	23.314352264551914
48-49	23.60345457630967	26.843829562752987	26.759484553672603	22.793231307264747
50-51	23.142588139724925	26.813249032527835	26.629908826095395	23.414254001651848
52-53	22.533942194705613	27.473115543580555	26.486172014006794	23.50677024770704
54-55	22.61076265058313	27.356489184931633	26.45213558442378	23.580612580061462
56-57	23.759148200649047	26.748935187493	26.208496142936365	23.28342046892159
58-59	22.623624025240282	27.161728805110226	27.139670612235733	23.074976557413756
60-61	22.668523088750796	27.78933308681325	26.3589333155888	23.183210508847154
62-63	23.123917516872204	27.006794928985656	26.039664426632896	23.829623127509244
64-65	22.189056341475265	27.265185909669544	26.564454510479194	23.981303238375997
66-67	23.05650211825382	26.77173512617425	26.4342535457727	23.737509209799228
68-69	22.369406629594998	26.905504080343157	26.74567006368151	23.979419226380337
70-71	22.39240268373245	26.539322565111867	26.94722613714028	24.121048614015404
72-73	22.99680575617748	26.734915981303292	27.318658629408315	22.949619633110913
74-75	23.177553374247537	27.051563016418445	26.817985748908697	22.952897860425324
76-77	22.82984169486589	27.028706511847705	26.48177876421497	23.65967302907144
78-79	22.60458085921041	26.89947021727769	26.669529319939496	23.826419603572408
80-81	22.21332230654502	27.237195518126285	27.328369129720247	23.221113045608448
82-83	23.07533007522129	26.251212295104143	28.292207870694664	22.381249758979905
84-85	23.308196845110945	26.785187207858257	26.855087105494967	23.051528841535827
86-87	21.971157211111645	27.525988808787936	27.34600341483001	23.156850565270407
88-89	23.121724379594845	26.118304058615127	27.14733580571398	23.612635756076042
90-91	22.745006664381123	26.224437568367755	27.42676606099733	23.60378970625379
92-93	22.93582681353049	26.45785569666766	27.327248439828942	23.2790690499729
94-95	21.664778206350636	26.502218091898776	28.03584816848128	23.797155533269308
96-97	22.081951491077294	26.415781786861476	27.478121046791482	24.024145675269747
98-99	21.400811759811376	27.227072873504945	27.362623270279084	24.009492096404593
100	22.32602296357695	26.40418951517875	26.546052836585503	24.723734684658798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1165.0
1	795.5
2	496.0
3	582.0
4	572.0
5	491.5
6	426.0
7	419.0
8	482.0
9	503.0
10	441.0
11	384.0
12	353.0
13	384.5
14	444.5
15	667.0
16	789.5
17	869.0
18	1153.0
19	1401.5
20	1692.0
21	2145.5
22	2971.5
23	4263.0
24	5804.5
25	7558.5
26	10077.5
27	12728.0
28	16529.0
29	25254.5
30	37306.5
31	48765.5
32	64876.0
33	80250.5
34	90984.5
35	102828.0
36	113748.5
37	135993.0
38	144239.0
39	130541.0
40	119783.5
41	110489.0
42	109311.5
43	116601.5
44	127554.5
45	154410.5
46	179767.5
47	196552.5
48	207284.5
49	200533.0
50	188816.0
51	193183.5
52	196662.5
53	188088.5
54	179713.0
55	156671.5
56	136335.5
57	112400.0
58	88542.0
59	83554.0
60	68983.0
61	48143.5
62	36346.0
63	30570.0
64	22625.0
65	11615.0
66	6809.0
67	4998.0
68	3755.0
69	2786.5
70	2171.0
71	1756.5
72	1383.0
73	1218.0
74	1017.0
75	781.5
76	561.5
77	353.5
78	230.0
79	153.5
80	102.0
81	67.5
82	49.5
83	36.5
84	33.5
85	24.5
86	16.0
87	15.0
88	13.5
89	11.0
90	11.5
91	14.0
92	12.5
93	10.5
94	6.5
95	5.5
96	8.5
97	7.0
98	5.0
99	7.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05771460301469593
2	0.007044542689468271
3	0.007182670977497059
4	0.009024381484547588
5	0.0034762285820578724
6	0.0027625657605757923
7	9.89919397539659E-4
8	0.0017266036003598703
9	7.827269654964746E-4
10-11	5.870452241223559E-4
12-13	3.3381002940290825E-4
14-15	2.7625657605757925E-4
16-17	7.251735121511455E-4
18-19	0.00150790047764762
20-21	7.251735121511455E-4
22-23	8.74812490849001E-4
24-25	0.0010244514695468563
26-27	0.003188461315331227
28-29	0.003706442395439188
30-31	0.004673340411640715
32-33	0.005605706355835045
34-35	0.0033035682220218855
36-37	0.007608566532252495
38-39	0.005916495003899822
40-41	0.005743834643863835
42-43	0.013651679133512042
44-45	0.019314938942692414
46-47	0.01787610260905919
48-49	0.020972478399037892
50-51	0.0177034422490232
52-53	0.023873172447642473
54-55	0.01833653023582182
56-57	0.015021451323130871
58-59	0.016817119067505135
60-61	0.015343750661864712
62-63	0.010946666826281577
64-65	0.010797027847583723
66-67	0.013536572226821383
68-69	0.005053193203719887
70-71	0.008276186591058311
72-73	0.00805748346834606
74-75	0.010854581300929051
76-77	0.010025811572756312
78-79	0.011464647906389538
80-81	0.009807108450044063
82-83	0.006227283651964598
84-85	0.009991279500749116
86-87	0.00874812490849001
88-89	0.0077351841296122185
90-91	0.012638738354634249
92-93	0.008149568993698586
94-95	0.0069639678547848095
96-97	0.009243084607259838
98-99	0.008863231815180667
100	0.2635717949402687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4343788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.74342223474493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65585079980934	49.82786972225795
2	15.176132714220406	20.258140672714763
3	5.237387262411134	10.486834483859434
4	2.2587014878078437	6.03013868412022
5	1.05592068850272	3.5237880179569805
6	0.5698341357197853	2.2819608194469945
7	0.3386651613058456	1.5822570300063838
8	0.21726695618531688	1.1600912155467544
9	0.13408557692528036	0.8054397248682023
>10	0.350788891972732	3.3073393945677383
>50	0.004051499527769417	0.17440988872451585
>100	9.294207729410119E-4	0.09774744303123238
>500	1.4452681508821244E-4	0.06646241606125146
>1k	1.9270241907813713E-4	0.19466060238732855
>5k	4.817560476953428E-5	0.202859884450224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6309	0.1452418948622723	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.3021381338131603E-5
7	0.0	0.0	0.0	0.0	2.3021381338131603E-5
8	0.0	0.0	0.0	0.0	2.3021381338131603E-5
9	0.0	0.0	0.0	0.0	2.3021381338131603E-5
10-11	0.0	0.0	0.0	0.0	2.3021381338131603E-5
12-13	0.0	0.0	0.0	0.0	8.057483468346062E-5
14-15	0.0	0.0	0.0	0.0	1.035962160215922E-4
16-17	0.0	2.3021381338131603E-5	0.0	0.0	1.1510690669065801E-4
18-19	0.0	2.3021381338131603E-5	0.0	0.0	1.9568174137411863E-4
20-21	0.0	2.3021381338131603E-5	0.0	0.0	2.0719243204318444E-4
22-23	0.0	2.3021381338131603E-5	0.0	3.4532072007197406E-5	2.0719243204318444E-4
24-25	0.0	2.3021381338131603E-5	0.0	1.1510690669065801E-4	2.0719243204318444E-4
26-27	0.0	2.3021381338131603E-5	0.0	3.4532072007197403E-4	2.3021381338131602E-4
28-29	0.0	2.3021381338131603E-5	0.0	0.0018992639603958575	2.3021381338131602E-4
30-31	0.0	2.3021381338131603E-5	0.0	0.00690641440143948	2.3021381338131602E-4
32-33	0.0	2.3021381338131603E-5	0.0	0.01784157053705199	2.3021381338131602E-4
34-35	0.0	2.3021381338131603E-5	0.0	0.03553350209540613	2.3021381338131602E-4
36-37	0.0	2.3021381338131603E-5	0.0	0.06609438582177583	2.3021381338131602E-4
38-39	0.0	2.3021381338131603E-5	0.0	0.10945515757214672	2.5323519471944763E-4
40-41	0.0	2.3021381338131603E-5	0.0	0.15992953615600025	2.7625657605757925E-4
42-43	0.0	2.3021381338131603E-5	0.0	0.2117046227854582	2.877672667266451E-4
44-45	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.2750249321559892	2.9927795739571086E-4
46-47	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.35460984744191015	3.222993387338425E-4
48-49	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.4515989270194586	3.4532072007197403E-4
50-51	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.5536181784193888	3.4532072007197403E-4
52-53	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.6545784462777648	3.4532072007197403E-4
54-55	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.7667386161571421	3.6834210141010565E-4
56-57	4.6042762676263206E-5	2.3021381338131603E-5	0.0	0.9053733745753706	3.798527920791714E-4
58-59	4.6042762676263206E-5	2.3021381338131603E-5	0.0	1.0689748210548027	3.9136348274823726E-4
60-61	4.6042762676263206E-5	2.3021381338131603E-5	0.0	1.2472293767559557	3.9136348274823726E-4
62-63	4.6042762676263206E-5	2.3021381338131603E-5	0.0	1.4328968172479872	4.374062454245005E-4
64-65	4.6042762676263206E-5	2.3021381338131603E-5	0.0	1.6256088004294869	4.6042762676263204E-4
66-67	4.6042762676263206E-5	2.3021381338131603E-5	0.0	1.8473737668597088	4.6042762676263204E-4
68-69	4.6042762676263206E-5	2.3021381338131603E-5	0.0	2.1032679311237104	4.6042762676263204E-4
70-71	4.6042762676263206E-5	2.3021381338131603E-5	0.0	2.390287002956866	4.6042762676263204E-4
72-73	4.6042762676263206E-5	2.3021381338131603E-5	0.0	2.688275762997642	4.719383174316979E-4
74-75	4.6042762676263206E-5	2.3021381338131603E-5	0.0	2.9821552064695607	5.064703894388953E-4
76-77	4.6042762676263206E-5	2.3021381338131603E-5	0.0	3.3195680820518865	5.294917707770268E-4
78-79	4.6042762676263206E-5	2.3021381338131603E-5	0.0	3.6923417993695824	5.294917707770268E-4
80-81	4.6042762676263206E-5	2.3021381338131603E-5	0.0	4.109627357504555	5.525131521151585E-4
82-83	4.6042762676263206E-5	2.3021381338131603E-5	0.0	4.54439765476584	5.755345334532901E-4
84-85	4.6042762676263206E-5	2.3021381338131603E-5	0.0	4.969176672526376	5.755345334532901E-4
86-87	4.6042762676263206E-5	2.3021381338131603E-5	0.0	5.424723766445323	5.870452241223559E-4
88	4.6042762676263206E-5	2.3021381338131603E-5	0.0	5.790015534828127	5.985559147914217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4475	0.0	40.743767	94
GTACTAG	4790	0.0	36.320198	1
GGTATCA	6365	0.0	34.055286	1
GTATCAA	9985	0.0	32.963398	1
CAACGCA	9120	0.0	32.670544	5
TAGGCAT	5930	0.0	31.304327	5
ATCAACG	9620	0.0	30.973919	3
GCATCGT	5155	0.0	30.537825	8
TATTCTC	2325	0.0	30.11794	5
TAGTACT	6665	0.0	29.686834	4
CTGTGCG	6780	0.0	29.110292	9
CAGTACG	770	0.0	28.683628	9
AGGGCTA	5650	0.0	28.280846	5
CTAGGCA	6835	0.0	28.260853	4
ACTGTGC	7415	0.0	27.440922	8
AACGCAG	11615	0.0	27.43105	6
GCTATTG	5745	0.0	27.40165	8
TATCAAC	12010	0.0	27.314571	2
CTATTGA	5790	0.0	27.188997	9
CTAGTAC	7930	0.0	26.729158	3
>>END_MODULE