FastQCFastQC Report
Mon 16 Jan 2017
ERR1146679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4343788
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141890.3266503798067493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123970.2853960644488175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77260.17786319221840477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.050.8303571
GTATCAA183100.042.24041
GTGGTAT24850.039.945751
GGGCACC43050.037.8013694
CAACGCA225750.033.1436045
ATCAACG227050.032.941633
TATCAAC245750.031.4910352
AACGCAG253650.030.478986
GTACTAG46600.029.983691
TCAACGC267250.028.3737554
TAGGCAT60100.027.9958065
TATTCTC27700.027.8258535
ACGCAGA268600.027.7152377
CGCAGAG271250.027.3757938
CAGTACG7750.027.293569
CTGTGCG61850.027.0557589
TAGTACT63100.026.2411484
TGGTATC37750.026.0461712
GCAGAGT285600.025.8069849
ACTGTGC68650.025.1933968
Produced by FastQC (version 0.11.5)