##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4343788
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14580131442879	33.0	31.0	34.0	30.0	34.0
2	32.203068842217895	33.0	31.0	34.0	30.0	34.0
3	32.34849651962757	34.0	31.0	34.0	30.0	34.0
4	35.96118802298823	37.0	35.0	37.0	35.0	37.0
5	35.87546767936188	37.0	35.0	37.0	35.0	37.0
6	35.919837478256305	37.0	35.0	37.0	35.0	37.0
7	35.84565429988756	37.0	35.0	37.0	35.0	37.0
8	35.85382228598633	37.0	35.0	37.0	35.0	37.0
9	37.5562695509081	39.0	37.0	39.0	35.0	39.0
10-11	37.498115815044386	39.0	37.0	39.0	34.5	39.0
12-13	37.46332383164187	39.0	37.0	39.0	34.0	39.0
14-15	38.66694771936383	40.0	38.0	41.0	34.0	41.0
16-17	38.671326155880536	40.0	38.0	41.0	34.0	41.0
18-19	38.74647519630332	40.0	38.0	41.0	34.0	41.0
20-21	38.747968938631445	40.0	38.0	41.0	34.0	41.0
22-23	38.71055919395698	40.0	38.0	41.0	34.0	41.0
24-25	38.626797394347975	40.0	38.0	41.0	34.0	41.0
26-27	38.47260697345266	40.0	38.0	41.0	34.0	41.0
28-29	38.414074650972836	40.0	38.0	41.0	34.0	41.0
30-31	38.265765157047255	40.0	37.5	41.0	34.0	41.0
32-33	38.101916230718444	40.0	37.0	41.0	33.0	41.0
34-35	37.98866807035702	40.0	37.0	41.0	33.0	41.0
36-37	37.97006345613552	40.0	37.0	41.0	33.0	41.0
38-39	37.86549585292836	40.0	37.0	41.0	33.0	41.0
40-41	37.739179375236546	40.0	37.0	41.0	33.0	41.0
42-43	37.58931248486344	40.0	36.0	41.0	32.5	41.0
44-45	37.35484662234897	40.0	36.0	41.0	32.0	41.0
46-47	37.06204411909605	40.0	35.5	41.0	31.0	41.0
48-49	36.748885419822514	39.0	35.0	41.0	30.5	41.0
50-51	36.52724499906533	39.0	35.0	41.0	30.0	41.0
52-53	36.34123730255712	39.0	35.0	41.0	30.0	41.0
54-55	36.156980842527304	38.5	35.0	41.0	30.0	41.0
56-57	35.92150342051684	38.0	35.0	40.0	29.0	41.0
58-59	35.63201944938381	37.5	35.0	40.0	29.0	41.0
60-61	35.447135311391804	37.0	35.0	40.0	29.0	41.0
62-63	35.2320939695952	36.5	34.5	40.0	29.0	41.0
64-65	34.98301563059707	36.0	34.0	39.5	29.0	41.0
66-67	34.73583103042782	35.5	34.0	39.0	28.5	41.0
68-69	34.48661398760713	35.0	34.0	39.0	28.0	41.0
70-71	34.27381354706998	35.0	34.0	38.5	28.0	41.0
72-73	34.048996060581224	35.0	34.0	37.0	28.0	40.0
74-75	33.794543955644244	35.0	34.0	37.0	28.0	39.5
76-77	33.561641129815726	35.0	33.5	36.5	27.0	39.0
78-79	33.347962999115055	35.0	33.0	36.0	27.0	39.0
80-81	33.22539302102221	35.0	33.0	36.0	27.0	38.5
82-83	33.0683829413406	35.0	33.0	35.5	27.0	37.0
84-85	32.94450903220876	35.0	33.0	35.0	27.0	37.0
86-87	32.80372119910088	35.0	33.0	35.0	27.0	36.5
88-89	32.70576499129331	35.0	33.0	35.0	27.0	36.0
90-91	32.64018064417509	35.0	33.0	35.0	27.0	36.0
92-93	32.576019363744265	35.0	33.0	35.0	27.0	36.0
94-95	32.52983605553494	35.0	33.0	35.0	27.0	35.5
96-97	32.44010446642424	35.0	33.0	35.0	27.0	35.0
98-99	32.364145879126696	35.0	33.0	35.0	27.0	35.0
100	31.153074919862572	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.422741724697282
1101	2	0.4378165665638676
1101	3	0.35392346700070476
1101	4	0.2900526382980857
1101	5	0.2541172337569719
1101	6	0.2685441458608082
1101	7	0.2911274438022815
1101	8	0.3042986509402894
1101	9	0.40522747579162655
1101	10-11	0.4247881999469385
1101	12-13	0.3961494976299065
1101	14-15	0.5522442754482526
1101	16-17	0.5536812453812132
1101	18-19	0.5205897258541015
1101	20-21	0.542933730430768
1101	22-23	0.4518393752682286
1101	24-25	0.49351701195516284
1101	26-27	0.5667048847019274
1101	28-29	0.5774945125296185
1101	30-31	0.6041343294535153
1101	32-33	0.5987095195897538
1101	34-35	0.6267202216438932
1101	36-37	0.585474212814475
1101	38-39	0.5914704308788501
1101	40-41	0.5661592488281002
1101	42-43	0.5568852704895022
1101	44-45	0.4780263469915056
1101	46-47	0.5509095883168698
1101	48-49	0.5912888883743221
1101	50-51	0.6307214711613014
1101	52-53	0.6359539409808974
1101	54-55	0.6215731875016317
1101	56-57	0.6230829214208242
1101	58-59	0.6383871805424093
1101	60-61	0.6411375255546758
1101	62-63	0.5824834459873287
1101	64-65	0.5829241698199255
1101	66-67	0.5569413743613083
1101	68-69	0.5517412231775438
1101	70-71	0.54696726161513
1101	72-73	0.539321127843138
1101	74-75	0.5105877274863388
1101	76-77	0.5336693252503224
1101	78-79	0.513293383850737
1101	80-81	0.5093932346857741
1101	82-83	0.4814048208370636
1101	84-85	0.45386741702035494
1101	86-87	0.4321238421616016
1101	88-89	0.4383723462142086
1101	90-91	0.3800858757984429
1101	92-93	0.3888449719136773
1101	94-95	0.44967276440040393
1101	96-97	0.44541214268736695
1101	98-99	0.46982766540168797
1101	100	0.8047473054617136
1102	1	0.3097278578956377
1102	2	0.38828112267637493
1102	3	0.3136767530159261
1102	4	0.20388179194849698
1102	5	0.2180279160922538
1102	6	0.15646770154395284
1102	7	0.21711677393238205
1102	8	0.23600204014122994
1102	9	0.25704484146445594
1102	10-11	0.24250850682216907
1102	12-13	0.2839329121141816
1102	14-15	0.42551363237264894
1102	16-17	0.4122102160799912
1102	18-19	0.3817147083836687
1102	20-21	0.467710140373363
1102	22-23	0.4613467531420383
1102	24-25	0.444388644883432
1102	26-27	0.4800719375438476
1102	28-29	0.4098940748559414
1102	30-31	0.43158311747767186
1102	32-33	0.5003273131150578
1102	34-35	0.5286410878247878
1102	36-37	0.5139681419178856
1102	38-39	0.5237702700879154
1102	40-41	0.4848912039174422
1102	42-43	0.5137462713217857
1102	44-45	0.4825269192662134
1102	46-47	0.4300422112489031
1102	48-49	0.5007077493902585
1102	50-51	0.45814029188507277
1102	52-53	0.4488579486666282
1102	54-55	0.419859825988965
1102	56-57	0.3983287980839805
1102	58-59	0.3518746062379847
1102	60-61	0.4087398519237624
1102	62-63	0.4316097354941135
1102	64-65	0.36554282081293366
1102	66-67	0.41511785154145997
1102	68-69	0.4396105733026374
1102	70-71	0.3820573964553944
1102	72-73	0.414820719255367
1102	74-75	0.42722663033059405
1102	76-77	0.3727719305167625
1102	78-79	0.4219053312341998
1102	80-81	0.3510830378711276
1102	82-83	0.32378922323496084
1102	84-85	0.35303393573921227
1102	86-87	0.4016681881000821
1102	88-89	0.37600378367510245
1102	90-91	0.353172658833941
1102	92-93	0.31259493886150125
1102	94-95	0.36500569733031085
1102	96-97	0.35864339832984626
1102	98-99	0.286572020405913
1102	100	0.5770448026884765
1103	1	0.2483344981887754
1103	2	0.33896377807143097
1103	3	0.2352814945906374
1103	4	0.22260883568147705
1103	5	0.19232880367358263
1103	6	0.2134528236138351
1103	7	0.22214542550072025
1103	8	0.2323376746113368
1103	9	0.3053865736948964
1103	10-11	0.30265432898286093
1103	12-13	0.2885749151230499
1103	14-15	0.3437582376651491
1103	16-17	0.40838730682956026
1103	18-19	0.3242184304280826
1103	20-21	0.29320618117214536
1103	22-23	0.3255196541212584
1103	24-25	0.3837697534402622
1103	26-27	0.4085976322156739
1103	28-29	0.41081940120275107
1103	30-31	0.39114788105038656
1103	32-33	0.46364186258787043
1103	34-35	0.44404659425461546
1103	36-37	0.39439687361204534
1103	38-39	0.3991112074038625
1103	40-41	0.45147963974139316
1103	42-43	0.378690468714332
1103	44-45	0.5006106629475227
1103	46-47	0.453702019073809
1103	48-49	0.3714305945693184
1103	50-51	0.41967999588800353
1103	52-53	0.4313284004405986
1103	54-55	0.3029645014427942
1103	56-57	0.2897772699108714
1103	58-59	0.38943243760973445
1103	60-61	0.3584650720867657
1103	62-63	0.3655245991265019
1103	64-65	0.41362375374684035
1103	66-67	0.46512734269369815
1103	68-69	0.41665037807280925
1103	70-71	0.41002101484372844
1103	72-73	0.3486274715880171
1103	74-75	0.3780186169678288
1103	76-77	0.330359094184729
1103	78-79	0.3858696464536706
1103	80-81	0.301674221981429
1103	82-83	0.2552943101509797
1103	84-85	0.2952392842596865
1103	86-87	0.2734187859938757
1103	88-89	0.332365832339093
1103	90-91	0.2812503174423142
1103	92-93	0.23430708416933754
1103	94-95	0.2739124483454276
1103	96-97	0.2686353369501475
1103	98-99	0.25492119916506084
1103	100	0.41483086089339594
1104	1	0.15799370104681287
1104	2	0.2338914719856504
1104	3	0.17906563008799026
1104	4	0.14519745667373485
1104	5	0.08744241719533363
1104	6	0.1216993382640581
1104	7	0.18614628057565596
1104	8	0.17696206290695926
1104	9	0.1907679847131334
1104	10-11	0.21731579785741673
1104	12-13	0.18339491199948554
1104	14-15	0.2245580306306607
1104	16-17	0.2173755664818131
1104	18-19	0.2387058907764299
1104	20-21	0.23479461435260163
1104	22-23	0.3177160279223372
1104	24-25	0.3253031797431021
1104	26-27	0.33189011078264485
1104	28-29	0.25625273143580074
1104	30-31	0.2708890675658324
1104	32-33	0.36918560490601493
1104	34-35	0.36361952837702205
1104	36-37	0.3636019979096403
1104	38-39	0.36643053582847074
1104	40-41	0.4089735390116829
1104	42-43	0.3863976175803785
1104	44-45	0.3181848230137305
1104	46-47	0.37805688846697905
1104	48-49	0.3602270096395017
1104	50-51	0.40738858475769746
1104	52-53	0.39924287053163
1104	54-55	0.3846289248962407
1104	56-57	0.38149348158151497
1104	58-59	0.35306356398708516
1104	60-61	0.28830017905531946
1104	62-63	0.311193159873433
1104	64-65	0.3567461813717401
1104	66-67	0.3164852957227069
1104	68-69	0.3531932189816658
1104	70-71	0.30303249232288465
1104	72-73	0.25746910364583897
1104	74-75	0.29802926513491457
1104	76-77	0.23675366614727977
1104	78-79	0.2797292244598282
1104	80-81	0.2952968082832683
1104	82-83	0.2086764726104562
1104	84-85	0.2088626453882938
1104	86-87	0.2523689699703482
1104	88-89	0.30838783101465594
1104	90-91	0.2679706625735321
1104	92-93	0.30137057178537674
1104	94-95	0.2977424223414218
1104	96-97	0.22764668614865258
1104	98-99	0.21024667516316242
1104	100	0.4119607512110157
1105	1	0.19423533496890855
1105	2	0.13999419119912204
1105	3	0.08670572256180265
1105	4	0.14595542650678084
1105	5	0.11195010846382303
1105	6	0.14309350528578335
1105	7	0.19761633262441336
1105	8	0.16485922668675812
1105	9	0.23996893262165742
1105	10-11	0.17920641022217154
1105	12-13	0.1796478939392685
1105	14-15	0.2730368155580436
1105	16-17	0.32292729798907516
1105	18-19	0.25444642410391083
1105	20-21	0.28256141184357375
1105	22-23	0.28017799832732493
1105	24-25	0.22310581776389427
1105	26-27	0.23297259389268277
1105	28-29	0.2540528412065939
1105	30-31	0.2620405293225332
1105	32-33	0.2510885693717526
1105	34-35	0.2398799728640597
1105	36-37	0.2962404570040462
1105	38-39	0.2786134557508291
1105	40-41	0.3282251308229007
1105	42-43	0.30311679775574163
1105	44-45	0.27271531712471386
1105	46-47	0.3072650824218144
1105	48-49	0.32290774222868635
1105	50-51	0.33497007475381224
1105	52-53	0.32964646246524154
1105	54-55	0.24581642876920995
1105	56-57	0.2496972641129389
1105	58-59	0.24039138077993982
1105	60-61	0.2588713249246126
1105	62-63	0.2344152348108608
1105	64-65	0.2566590541719478
1105	66-67	0.2255929398998333
1105	68-69	0.23945824462884246
1105	70-71	0.28549280000056143
1105	72-73	0.2740125788652392
1105	74-75	0.24675564536215688
1105	76-77	0.24244209635564573
1105	78-79	0.15385382691596305
1105	80-81	0.1469495741454807
1105	82-83	0.2007990133039783
1105	84-85	0.1216005655011756
1105	86-87	0.19071143531384394
1105	88-89	0.20052107888302828
1105	90-91	0.18640694788291512
1105	92-93	0.1617318478862515
1105	94-95	0.16741641472675184
1105	96-97	0.20412299170713055
1105	98-99	0.22504386573134383
1105	100	0.3915555605381478
1106	1	0.13184251854383433
1106	2	0.13810081430574428
1106	3	0.12946238830234336
1106	4	0.08367099123847765
1106	5	0.0901666935352452
1106	6	0.12118434426371749
1106	7	0.1701384160335877
1106	8	0.12153909868921176
1106	9	0.13034229073694803
1106	10-11	0.1220057098763121
1106	12-13	0.0784585155321409
1106	14-15	0.13295955955337035
1106	16-17	0.11788062822466117
1106	18-19	0.15733571005771552
1106	20-21	0.20726105831740682
1106	22-23	0.22498115179983813
1106	24-25	0.24288777403778994
1106	26-27	0.24681693939025706
1106	28-29	0.23018144233519422
1106	30-31	0.2261322353497235
1106	32-33	0.2533496038908609
1106	34-35	0.23354057337949286
1106	36-37	0.1565703788258972
1106	38-39	0.18694126024798408
1106	40-41	0.10884490337816288
1106	42-43	0.17085166750674574
1106	44-45	0.22666769648031249
1106	46-47	0.1820192787485908
1106	48-49	0.16901218813151786
1106	50-51	0.23550611238662356
1106	52-53	0.2771842940756599
1106	54-55	0.1434537195516583
1106	56-57	0.14494507972366222
1106	58-59	0.10708225855952236
1106	60-61	0.18066264389334918
1106	62-63	0.25277536204195883
1106	64-65	0.16401741616740395
1106	66-67	0.1395900968405357
1106	68-69	0.20904206098685307
1106	70-71	0.20813589905495178
1106	72-73	0.13569277421478887
1106	74-75	0.1425154272106539
1106	76-77	0.1111035950574717
1106	78-79	0.1751124016019645
1106	80-81	0.10442493988697521
1106	82-83	0.13697508657682533
1106	84-85	0.15390114417272116
1106	86-87	0.09637641658366647
1106	88-89	0.1514049541620679
1106	90-91	0.1750314173541625
1106	92-93	0.16548237482226824
1106	94-95	0.14127050854052214
1106	96-97	0.1529682832943564
1106	98-99	0.10627069252267773
1106	100	0.26994172319850307
1107	1	0.1533737973972933
1107	2	0.17797869623383633
1107	3	0.1302778964400204
1107	4	0.1298672748824572
1107	5	0.030698597456272125
1107	6	0.008676220249320465
1107	7	0.06644057593407382
1107	8	0.11575815566977354
1107	9	0.12781064280780896
1107	10-11	0.1938271232249278
1107	12-13	0.08807374481263963
1107	14-15	0.13524919573421812
1107	16-17	0.11948306288149269
1107	18-19	0.11636054251351169
1107	20-21	0.14395121990297355
1107	22-23	0.1592316237072069
1107	24-25	0.14100781807833584
1107	26-27	0.09646923778731775
1107	28-29	0.1876934683618643
1107	30-31	0.16493168931653912
1107	32-33	0.22311972690366133
1107	34-35	0.21518853358394807
1107	36-37	0.1344376433040395
1107	38-39	0.19990178157670613
1107	40-41	0.21514429210432695
1107	42-43	0.24739463997021716
1107	44-45	0.20926273346865543
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2216	68-69	-0.6140553692534283
2216	70-71	-0.7072808913618331
2216	72-73	-0.6779996466204921
2216	74-75	-0.579969616702968
2216	76-77	-0.5912473037219996
2216	78-79	-0.6383166082556215
2216	80-81	-0.6651859190096019
2216	82-83	-0.6969681580807006
2216	84-85	-0.6363194139049781
2216	86-87	-0.6211444795086578
2216	88-89	-0.5982326347612315
2216	90-91	-0.6095974029889462
2216	92-93	-0.6545973202574302
2216	94-95	-0.6560762888062754
2216	96-97	-0.624642137173204
2216	98-99	-0.6549410686890766
2216	100	-0.10589447406626462
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	27.0
13	75.0
14	280.0
15	821.0
16	2023.0
17	3959.0
18	7201.0
19	11390.0
20	16275.0
21	23539.0
22	33825.0
23	40004.0
24	39924.0
25	37092.0
26	36303.0
27	41738.0
28	51533.0
29	65027.0
30	80282.0
31	100872.0
32	127770.0
33	165048.0
34	237078.0
35	352962.0
36	555380.0
37	1049809.0
38	908041.0
39	354674.0
40	832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.94711252022429	22.35548788292615	14.279679395034933	24.41772020181464
2	14.01293257068341	20.788833397951674	40.49707467700806	24.701159354356857
3	17.007482869790145	27.48347755461362	30.009452579177438	25.499586996418795
4	11.601713327552808	17.916665034509187	39.24758845851905	31.23403317941896
5	12.389518054564629	38.93859055991333	35.047770469679065	13.62412091584298
6	29.07177538861827	39.52555684705535	17.603280271339273	13.799387492987112
7	25.566832451307477	32.9901919706947	23.895572251684474	17.547403326313347
8	26.455964241348795	34.5309209381305	19.508871059084836	19.504243761435873
9	26.15118417381327	14.522002455000106	21.71524024653137	37.61157312465526
10-11	25.45329099854781	26.179454430096495	27.206564869187904	21.160689702167783
12-13	26.061053374566306	24.05408846180498	27.859017955127953	22.025840208500767
14-15	23.317041454326773	24.818053168616398	26.12876336476476	25.736142012292067
16-17	21.314265337074463	27.204159134838076	27.449198717801142	24.032376810286323
18-19	20.816034675741392	26.859840701176346	30.629144713851	21.694979909231254
20-21	22.455112910666912	26.141802960917982	29.801224184974036	21.60185994344107
22-23	22.86226906101311	25.67190203573471	29.64205435440219	21.82377454884999
24-25	22.30652140481994	25.86735356329545	30.33404254535442	21.49208248653019
26-27	22.135521132191666	26.165794252135953	29.333985605879658	22.364699009792723
28-29	21.212695002610623	26.625539736285475	29.972100387956317	22.189664873147585
30-31	22.22941128802787	26.094563086412137	29.781540903929933	21.89448472163006
32-33	22.77783814495551	26.42510407966503	28.91026219511634	21.886795580263126
34-35	22.560159473712805	26.29585053414209	29.720499711311877	21.423490280833228
36-37	22.276006563856246	25.89625690756547	29.373866772503632	22.453869756074653
38-39	22.411798181679217	25.8305308638451	30.0855612658813	21.67210968859438
40-41	21.9336901340489	25.1396131671251	30.60493513956022	22.321761559265784
42-43	22.223920688578723	25.38220097297566	29.543718524016366	22.85015981442925
44-45	22.441875616397486	26.43216013304517	29.055711282410652	22.070252968146693
46-47	22.529391397554395	26.28315424233411	28.195183558681965	22.992270801429534
48-49	23.50333395644539	26.04325993810011	28.083196049162616	22.37021005629188
50-51	22.820531296647072	26.23462517047333	27.6407366105344	23.3041069223452
52-53	22.104911657751252	26.794954081552785	27.56482360557191	23.535310655124054
54-55	22.299097009338393	26.777446321045133	27.354891629149492	23.568565040466986
56-57	23.399622633517104	26.166965330720558	27.08757885974177	23.345833176020562
58-59	22.2299599669551	26.614187875025625	27.976398011274267	23.179454146745012
60-61	22.42789012723457	27.15577970195599	27.181483074220015	23.234847096589426
62-63	22.92433470510071	26.343804071469417	26.823857425822805	23.908003797607066
64-65	21.968587756665617	26.610404661005244	27.313535848007177	24.107471734321965
66-67	22.72598195834666	26.116794778927073	27.25666564950378	23.90055761322249
68-69	22.26659081888895	25.996089127738276	27.667291773907937	24.070028279464836
70-71	22.28365504703404	25.70179261374166	27.741703115428102	24.2728492237962
72-73	22.91085326551515	25.810983129635517	27.980745301720557	23.29741830312878
74-75	23.025500193265252	26.379820667341807	27.451021243983625	23.143657895409312
76-77	22.7038557644007	26.420409679859624	26.913229070887994	23.96250548485168
78-79	22.409733995904265	26.36556838141101	27.223057578911853	24.00164004377287
80-81	22.000026244374727	26.867252729645188	27.780257692134146	23.352463333845943
82-83	22.93950579540254	25.84066027163388	28.710989118253472	22.50884481471011
84-85	23.23828879309948	26.416194805087173	27.105696686854884	23.239819714958465
86-87	21.8401427509814	27.022992374397646	27.68963402449659	23.447230850124363
88-89	22.795978993449957	25.792557095327854	27.48813938433459	23.923324526887594
90-91	22.36111661066332	25.817489251317056	27.84083845712544	23.980555680894184
92-93	22.64276018995402	26.25122358641812	27.410534307843754	23.695481915784104
94-95	21.429153540642407	26.202234086930577	28.08343777366667	24.285174598760346
96-97	21.945212335408634	26.100088217933287	27.539868428201373	24.414831018456702
98-99	21.27316066069523	26.86455922802862	27.48412215329109	24.37815795798506
100	22.044827128664334	26.136665198505792	26.62774658191877	25.190761090911103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	820.0
1	522.5
2	346.5
3	437.5
4	290.5
5	225.5
6	437.0
7	1159.0
8	2048.5
9	2210.5
10	1553.0
11	977.0
12	928.0
13	1030.5
14	1259.0
15	1786.0
16	2292.5
17	2921.0
18	3981.5
19	5163.0
20	6583.5
21	8085.0
22	9698.0
23	11456.5
24	13186.5
25	14801.5
26	16931.0
27	18912.5
28	22018.0
29	29941.5
30	40819.5
31	50762.5
32	65901.0
33	80034.0
34	88568.5
35	99751.0
36	110049.5
37	131013.5
38	139757.5
39	126710.0
40	116415.0
41	107982.0
42	106451.0
43	113105.0
44	124224.5
45	151802.5
46	176283.0
47	192534.0
48	205155.5
49	198020.0
50	185919.5
51	188615.5
52	190472.0
53	183810.0
54	179149.0
55	156166.5
56	134447.0
57	111704.5
58	87732.5
59	83876.5
60	69108.0
61	46924.0
62	34529.0
63	28644.5
64	21068.0
65	10321.5
66	5907.0
67	4414.0
68	3326.5
69	2421.0
70	1870.0
71	1524.5
72	1220.0
73	1092.5
74	896.5
75	693.0
76	488.0
77	263.5
78	133.5
79	61.5
80	33.0
81	15.0
82	5.5
83	1.5
84	0.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0077351841296122185
3	0.0
4	0.09045100727751906
5	0.004719383174316979
6	2.3021381338131603E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.4532072007197403E-4
14-15	0.009611426708669945
16-17	0.0
18-19	4.6042762676263206E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.1510690669065801E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.4532072007197406E-5
60-61	0.0
62-63	0.0
64-65	9.208552535252641E-5
66-67	1.4963897869785543E-4
68-69	0.0
70-71	4.374062454245005E-4
72-73	3.913634827482373E-4
74-75	3.913634827482373E-4
76-77	1.3812828802878962E-4
78-79	3.4532072007197406E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0634469269678907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4343788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.371285581089275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62765799399385	46.025080558230655
2	16.400573468379225	20.786508499165915
3	5.533738780491243	10.520404217689777
4	2.419343558471628	6.132676462506971
5	1.2153873653975351	3.8510329912127452
6	0.6448139643756795	2.4517613929875304
7	0.3837722251090167	1.7024097492831503
8	0.22235077735678171	1.1272523688843048
9	0.1381211284011784	0.787762212542404
>10	0.40092092010414254	3.6579929719630635
>50	0.006544889993494083	0.2890984443244645
>100	0.005303970792859034	0.6926672430252846
>500	8.115625577850619E-4	0.36158337441774846
>1k	5.072265966263283E-4	0.5108582587846193
>5k	5.072265966263283E-5	0.24834146525954626
>10k+	1.0144531932526565E-4	0.8545697897217572
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14189	0.3266503798067493	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12397	0.2853960644488175	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7726	0.17786319221840477	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.3812828802878962E-4
14-15	0.0	0.0	0.0	0.0	1.7266036003598702E-4
16-17	0.0	0.0	0.0	0.0	1.8417105070505282E-4
18-19	0.0	0.0	0.0	0.0	1.8417105070505282E-4
20-21	0.0	0.0	0.0	0.0	1.8417105070505282E-4
22-23	0.0	0.0	0.0	3.4532072007197406E-5	1.8417105070505282E-4
24-25	0.0	0.0	0.0	9.208552535252641E-5	1.8417105070505282E-4
26-27	0.0	0.0	0.0	3.222993387338424E-4	2.3021381338131602E-4
28-29	0.0	0.0	0.0	0.0018647318883886596	2.3021381338131602E-4
30-31	0.0	0.0	0.0	0.00696396785478481	2.3021381338131602E-4
32-33	0.0	0.0	0.0	0.017968188134411714	2.4172450405038183E-4
34-35	0.0	0.0	0.0	0.03562558762075865	2.5323519471944763E-4
36-37	0.0	0.0	0.0	0.06607136444043771	2.5323519471944763E-4
38-39	0.0	0.0	0.0	0.10968537138552803	2.5323519471944763E-4
40-41	0.0	0.0	0.0	0.1604475172361082	2.5323519471944763E-4
42-43	0.0	0.0	0.0	0.21257943527630724	2.5323519471944763E-4
44-45	0.0	0.0	0.0	0.27610693707888134	2.5323519471944763E-4
46-47	0.0	0.0	0.0	0.356163790682234	2.5323519471944763E-4
48-49	0.0	0.0	0.0	0.45356725512386886	2.5323519471944763E-4
50-51	0.0	0.0	0.0	0.5556901027398207	2.5323519471944763E-4
52-53	0.0	0.0	0.0	0.6570877768436213	2.7625657605757925E-4
54-55	0.0	0.0	0.0	0.7700536950698331	2.7625657605757925E-4
56-57	0.0	0.0	0.0	0.9098280118642992	2.7625657605757925E-4
58-59	0.0	0.0	0.0	1.0752136153974365	2.7625657605757925E-4
60-61	0.0	0.0	0.0	1.2550221143389133	2.9927795739571086E-4
62-63	0.0	0.0	0.0	1.4421974553085923	2.9927795739571086E-4
64-65	0.0	0.0	0.0	1.6359914434129843	2.9927795739571086E-4
66-67	0.0	0.0	0.0	1.8596211417315947	2.9927795739571086E-4
68-69	0.0	0.0	0.0	2.1180016151801144	2.9927795739571086E-4
70-71	0.0	0.0	0.0	2.4072882930750765	2.9927795739571086E-4
72-73	0.0	0.0	0.0	2.706819485665507	2.9927795739571086E-4
74-75	0.0	0.0	0.0	3.0027017893138432	2.9927795739571086E-4
76-77	0.0	0.0	0.0	3.3434642758808675	2.9927795739571086E-4
78-79	0.0	0.0	0.0	3.719322858297873	2.9927795739571086E-4
80-81	0.0	0.0	0.0	4.14037241228163	2.9927795739571086E-4
82-83	0.0	0.0	0.0	4.580529712776038	3.4532072007197403E-4
84-85	0.0	0.0	0.0	5.010327391668286	3.4532072007197403E-4
86-87	0.0	0.0	0.0	5.471365085036378	3.4532072007197403E-4
88	0.0	0.0	0.0	5.840915808966736	3.6834210141010565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10005	0.0	50.830357	1
GTATCAA	18310	0.0	42.2404	1
GTGGTAT	2485	0.0	39.94575	1
GGGCACC	4305	0.0	37.80136	94
CAACGCA	22575	0.0	33.143604	5
ATCAACG	22705	0.0	32.94163	3
TATCAAC	24575	0.0	31.491035	2
AACGCAG	25365	0.0	30.47898	6
GTACTAG	4660	0.0	29.98369	1
TCAACGC	26725	0.0	28.373755	4
TAGGCAT	6010	0.0	27.995806	5
TATTCTC	2770	0.0	27.825853	5
ACGCAGA	26860	0.0	27.715237	7
CGCAGAG	27125	0.0	27.375793	8
CAGTACG	775	0.0	27.29356	9
CTGTGCG	6185	0.0	27.055758	9
TAGTACT	6310	0.0	26.241148	4
TGGTATC	3775	0.0	26.046171	2
GCAGAGT	28560	0.0	25.806984	9
ACTGTGC	6865	0.0	25.193396	8
>>END_MODULE