FastQCFastQC Report
Mon 16 Jan 2017
ERR1146678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5819449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC51550.039.7539394
GTATCAA122000.034.993981
CAACGCA110000.034.7326165
ATCAACG118500.032.4414863
CTGTGCG71450.030.648189
TAGTACT73300.030.0059684
TACCCCG4250.029.8548225
TATCAAC142350.029.746822
ACTGTGC76750.028.8378938
GTACTAG62650.028.7438581
TATTCTC37650.028.7079725
GGTATCA73950.028.4844071
ACGCAGA133150.028.3750027
AACGCAG144750.028.2764116
CGCAGAG133150.028.0573718
TAGGCAT70500.027.5963025
TACATGG255000.027.4427572
CGTTAAC13050.027.382331
GTACATG258400.027.3121281
ACATGGG252150.026.783273
Produced by FastQC (version 0.11.5)