##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146678_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5819449
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.607279486425604	31.0	31.0	34.0	28.0	34.0
2	31.665300615230066	33.0	31.0	34.0	28.0	34.0
3	31.75116716376413	33.0	31.0	34.0	28.0	34.0
4	35.159060075962515	37.0	35.0	37.0	32.0	37.0
5	35.28689760834746	37.0	35.0	37.0	32.0	37.0
6	35.362728498866474	37.0	35.0	37.0	32.0	37.0
7	35.24995304538282	37.0	35.0	37.0	32.0	37.0
8	35.25156934960681	37.0	35.0	37.0	32.0	37.0
9	36.764459659325134	39.0	37.0	39.0	32.0	39.0
10-11	36.612523969193646	39.0	37.0	39.0	32.0	39.0
12-13	36.56567580538982	39.0	37.0	39.0	32.0	39.0
14-15	37.777199181572	40.0	38.0	41.0	32.0	41.0
16-17	37.84118702646934	40.0	38.0	41.0	32.0	41.0
18-19	37.819013191798746	40.0	38.0	41.0	32.0	41.0
20-21	37.89319684733039	40.0	38.0	41.0	32.0	41.0
22-23	37.853163332129895	40.0	38.0	41.0	32.0	41.0
24-25	37.82818949010465	40.0	37.0	41.0	32.0	41.0
26-27	37.87545513329526	40.0	37.0	41.0	32.0	41.0
28-29	37.88065141562371	40.0	37.0	41.0	32.0	41.0
30-31	37.752952298404885	40.0	37.0	41.0	32.0	41.0
32-33	37.74273303194168	40.0	37.0	41.0	32.0	41.0
34-35	37.67818155980059	40.0	37.0	41.0	31.0	41.0
36-37	37.56496053148675	40.0	37.0	41.0	31.0	41.0
38-39	37.45872976977717	40.0	36.0	41.0	31.0	41.0
40-41	37.36668746474108	40.0	36.0	41.0	31.0	41.0
42-43	37.20496399229549	40.0	36.0	41.0	30.0	41.0
44-45	37.0429415224706	39.5	35.5	41.0	30.0	41.0
46-47	37.02915765736584	39.5	35.0	41.0	30.0	41.0
48-49	36.88955646831856	39.0	35.0	41.0	30.0	41.0
50-51	36.70735115987785	39.0	35.0	41.0	30.0	41.0
52-53	36.479924130274185	39.0	35.0	41.0	30.0	41.0
54-55	36.273862181797625	38.5	35.0	40.5	29.0	41.0
56-57	36.018430009439044	38.0	35.0	40.0	29.0	41.0
58-59	35.72005304969594	37.5	34.0	40.0	28.5	41.0
60-61	35.464415531436046	37.0	34.0	40.0	28.0	41.0
62-63	35.1557975677766	36.5	34.0	40.0	28.0	41.0
64-65	35.00805514405229	36.0	34.0	40.0	28.0	41.0
66-67	34.75562359941637	35.5	34.0	39.0	28.0	41.0
68-69	34.632218960935994	35.0	34.0	39.0	28.0	41.0
70-71	34.547375533319396	35.0	34.0	39.0	28.0	41.0
72-73	34.32851237290678	35.0	34.0	38.5	28.0	41.0
74-75	34.06668560889527	35.0	33.5	37.0	28.0	40.0
76-77	33.80483556089245	35.0	33.0	37.0	27.0	39.0
78-79	33.53981459413082	35.0	33.0	36.5	27.0	39.0
80-81	33.339647018128346	35.0	33.0	36.0	27.0	39.0
82-83	33.059473414063774	35.0	33.0	36.0	26.0	37.0
84-85	32.848453092380396	35.0	33.0	35.5	26.0	37.0
86-87	32.65278379447951	35.0	33.0	35.0	26.0	37.0
88-89	32.50725455279357	35.0	33.0	35.0	26.0	36.0
90-91	32.33304751016806	35.0	33.0	35.0	25.0	36.0
92-93	32.16277486064402	35.0	32.5	35.0	25.0	36.0
94-95	32.06602601036627	35.0	32.0	35.0	25.0	36.0
96-97	31.92619954225907	35.0	32.0	35.0	25.0	35.0
98-99	31.81911680985605	35.0	32.0	35.0	24.5	35.0
100	30.883405628264807	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28334401286965516
1101	2	0.3780224507510681
1101	3	0.3927200936752442
1101	4	0.49166609520803917
1101	5	0.42046541531369286
1101	6	0.41452553810758985
1101	7	0.44648526939898403
1101	8	0.4287099021859646
1101	9	0.5899265467659731
1101	10-11	0.6234869968528827
1101	12-13	0.5491067824877405
1101	14-15	0.7578151922260759
1101	16-17	0.6864275032540021
1101	18-19	0.7108634266136846
1101	20-21	0.6617145428798423
1101	22-23	0.6667635815252453
1101	24-25	0.716590243829863
1101	26-27	0.6381898041944396
1101	28-29	0.6384357044342721
1101	30-31	0.6854746004978196
1101	32-33	0.6278123623506033
1101	34-35	0.6804452583132559
1101	36-37	0.7055463786936045
1101	38-39	0.7078724816229425
1101	40-41	0.710124963157476
1101	42-43	0.708823519569421
1101	44-45	0.6727006829709623
1101	46-47	0.7051864706241631
1101	48-49	0.6731018611402533
1101	50-51	0.6848811389113436
1101	52-53	0.6034189393244205
1101	54-55	0.587015890172303
1101	56-57	0.6119238348450722
1101	58-59	0.6342179368375582
1101	60-61	0.6178626180142786
1101	62-63	0.6241558646899463
1101	64-65	0.5923258951964883
1101	66-67	0.6337106847401444
1101	68-69	0.638818485511031
1101	70-71	0.6128817915500235
1101	72-73	0.6492709805708898
1101	74-75	0.6221992061521604
1101	76-77	0.6291416242744816
1101	78-79	0.5684813758791805
1101	80-81	0.5438844128290867
1101	82-83	0.5597876506938491
1101	84-85	0.572287438621025
1101	86-87	0.49558063637046956
1101	88-89	0.5201486649701934
1101	90-91	0.5247610081463705
1101	92-93	0.5264308484060223
1101	94-95	0.6030597815076604
1101	96-97	0.5554428363934072
1101	98-99	0.531413847158511
1101	100	0.523896051851338
1102	1	0.23783634489970495
1102	2	0.34515235939709044
1102	3	0.33223429524942105
1102	4	0.4205611896833119
1102	5	0.25378798769381916
1102	6	0.29537443404309727
1102	7	0.2585534396344116
1102	8	0.3007471590248585
1102	9	0.4327947979039166
1102	10-11	0.5095959521553581
1102	12-13	0.42841680009166794
1102	14-15	0.5786430884870128
1102	16-17	0.5433193541857122
1102	18-19	0.5387824809249864
1102	20-21	0.5348897937790653
1102	22-23	0.5437798414805997
1102	24-25	0.592385151101773
1102	26-27	0.5257915859105751
1102	28-29	0.5268617689638901
1102	30-31	0.5993747253310744
1102	32-33	0.5003743319511287
1102	34-35	0.5242084052054778
1102	36-37	0.5344822035318373
1102	38-39	0.5580416828817363
1102	40-41	0.5132211033741285
1102	42-43	0.5109744325417154
1102	44-45	0.5187003714925353
1102	46-47	0.4863565949680648
1102	48-49	0.5348263724270694
1102	50-51	0.4550273644496343
1102	52-53	0.3898234456022749
1102	54-55	0.4564982424261217
1102	56-57	0.4949527076299489
1102	58-59	0.45027280765123123
1102	60-61	0.4573565195309044
1102	62-63	0.4883388041196781
1102	64-65	0.4731251108811634
1102	66-67	0.4501679283719042
1102	68-69	0.4363797434000958
1102	70-71	0.4288314407088336
1102	72-73	0.395919191737633
1102	74-75	0.4273135367833447
1102	76-77	0.43607775017131445
1102	78-79	0.38970383008729215
1102	80-81	0.4133155032510558
1102	82-83	0.4162582428694179
1102	84-85	0.45183022773729675
1102	86-87	0.39169225431110277
1102	88-89	0.3902525785645139
1102	90-91	0.3910649407568769
1102	92-93	0.44110418028925835
1102	94-95	0.4468830284786094
1102	96-97	0.4802883391863837
1102	98-99	0.40691792959125905
1102	100	0.5153071365758883
1103	1	0.24929088558940649
1103	2	0.3036285140234867
1103	3	0.30693981288143846
1103	4	0.445594945638625
1103	5	0.17299972732119784
1103	6	0.25710602074274647
1103	7	0.26161723556082705
1103	8	0.2921476544497139
1103	9	0.36222534521198924
1103	10-11	0.4940889844616052
1103	12-13	0.37063820401778713
1103	14-15	0.5503101234365602
1103	16-17	0.5124847705766626
1103	18-19	0.5315725989919144
1103	20-21	0.4463130204969801
1103	22-23	0.5247360180014624
1103	24-25	0.4816998650055311
1103	26-27	0.4321451654712902
1103	28-29	0.5123723936768911
1103	30-31	0.524841739791789
1103	32-33	0.46416745901824896
1103	34-35	0.4836443784818414
1103	36-37	0.48617262196273714
1103	38-39	0.5421325324120687
1103	40-41	0.49878198232142523
1103	42-43	0.4920263526852864
1103	44-45	0.3977402907760492
1103	46-47	0.4077804143136987
1103	48-49	0.4184787370116325
1103	50-51	0.46432231317690764
1103	52-53	0.44106363076418376
1103	54-55	0.44108426367852616
1103	56-57	0.46907631845401454
1103	58-59	0.4558282457401006
1103	60-61	0.4612453038578934
1103	62-63	0.38480346712892555
1103	64-65	0.3631767379866986
1103	66-67	0.44543695752859236
1103	68-69	0.3827691962945323
1103	70-71	0.38260886165259933
1103	72-73	0.34552886151594464
1103	74-75	0.37063219538391934
1103	76-77	0.33913502421486896
1103	78-79	0.3179184482772115
1103	80-81	0.24847364848713482
1103	82-83	0.2939912542579961
1103	84-85	0.3080138512001085
1103	86-87	0.3039193998765555
1103	88-89	0.30023220587018074
1103	90-91	0.2865719441869743
1103	92-93	0.2997657469825086
1103	94-95	0.28502031917530246
1103	96-97	0.30468344199686825
1103	98-99	0.28516092839468854
1103	100	0.3922963852966781
1104	1	0.1340004727873385
1104	2	0.2658837619540968
1104	3	0.2639009285956533
1104	4	0.3575370176127848
1104	5	0.28277087431914083
1104	6	0.26839773502229036
1104	7	0.2261647286925026
1104	8	0.2676349781691627
1104	9	0.3149182900947949
1104	10-11	0.45818380243046164
1104	12-13	0.3749335771679725
1104	14-15	0.410037291588381
1104	16-17	0.4302113574244615
1104	18-19	0.35879778571413823
1104	20-21	0.35925903797166825
1104	22-23	0.364211324435054
1104	24-25	0.4342320039630252
1104	26-27	0.4098755691113709
1104	28-29	0.343583884402058
1104	30-31	0.327987291037779
1104	32-33	0.37712445797377114
1104	34-35	0.40962062418927303
1104	36-37	0.32726162392765445
1104	38-39	0.36342443827214765
1104	40-41	0.4281653713789453
1104	42-43	0.4624937372109059
1104	44-45	0.36792758138699355
1104	46-47	0.3243387832922977
1104	48-49	0.3087548765347634
1104	50-51	0.2988035596654868
1104	52-53	0.322546991201051
1104	54-55	0.3623693266206658
1104	56-57	0.2942115838364501
1104	58-59	0.3251208129234726
1104	60-61	0.3216187123105527
1104	62-63	0.3778630918597088
1104	64-65	0.33200751140895335
1104	66-67	0.3245571826748872
1104	68-69	0.2889204026591514
1104	70-71	0.28089050070131805
1104	72-73	0.3217176504771757
1104	74-75	0.21728859643882004
1104	76-77	0.20735585179196647
1104	78-79	0.20238384967971257
1104	80-81	0.2523242584329779
1104	82-83	0.30403526062396935
1104	84-85	0.32115278286237725
1104	86-87	0.33252292914984594
1104	88-89	0.28393748769255467
1104	90-91	0.34218618524651845
1104	92-93	0.3287909651197012
1104	94-95	0.33749359210326446
1104	96-97	0.2669608381189441
1104	98-99	0.2537740038824978
1104	100	0.33308910955492266
1105	1	0.14169505692675344
1105	2	0.23522101158487985
1105	3	0.23767671266632107
1105	4	0.3176528926288924
1105	5	0.17428134437735565
1105	6	0.18250810224903802
1105	7	0.21794770545411524
1105	8	0.16895816167185274
1105	9	0.36551588226416953
1105	10-11	0.32056921942210437
1105	12-13	0.23098365702000478
1105	14-15	0.4241043842721979
1105	16-17	0.32601597564664075
1105	18-19	0.3658611587495173
1105	20-21	0.3482375476495463
1105	22-23	0.36909089983191734
1105	24-25	0.34350698119028067
1105	26-27	0.3249532196592142
1105	28-29	0.3735701841039827
1105	30-31	0.41533150736838564
1105	32-33	0.3556898275028644
1105	34-35	0.391317713853617
1105	36-37	0.42505833593328646
1105	38-39	0.4083209566640136
1105	40-41	0.4017105687204534
1105	42-43	0.43407396245901
1105	44-45	0.40777578894120126
1105	46-47	0.4512766295616828
1105	48-49	0.3068469258585864
1105	50-51	0.3555228102392505
1105	52-53	0.39504309505907287
1105	54-55	0.3976602923887498
1105	56-57	0.4161227468936701
1105	58-59	0.3886952215460582
1105	60-61	0.4198271484376974
1105	62-63	0.3815756918456188
1105	64-65	0.42435855404928446
1105	66-67	0.385080628536727
1105	68-69	0.35670472952476473
1105	70-71	0.3711922241977703
1105	72-73	0.3662188084336151
1105	74-75	0.28373694374373315
1105	76-77	0.3378551595977157
1105	78-79	0.39748939469697575
1105	80-81	0.3076329937172346
1105	82-83	0.34182318019714586
1105	84-85	0.2746703097963845
1105	86-87	0.26208413680448217
1105	88-89	0.303401179353358
1105	90-91	0.31614425044506334
1105	92-93	0.347582987754528
1105	94-95	0.3883283371461488
1105	96-97	0.3130914609295168
1105	98-99	0.2828901206539811
1105	100	0.407199534106244
1106	1	0.1857742026446303
1106	2	0.21485860288046155
1106	3	0.1895974474909785
1106	4	0.31336220429594874
1106	5	0.15697827941702513
1106	6	0.23825297686126845
1106	7	0.19640550980630422
1106	8	0.21027077475597622
1106	9	0.3292093817460966
1106	10-11	0.37093909944562853
1106	12-13	0.2613927497417663
1106	14-15	0.39886376668037826
1106	16-17	0.3344707967260021
1106	18-19	0.3681159941324026
1106	20-21	0.36347751581861587
1106	22-23	0.3772214428548466
1106	24-25	0.40436981874618283
1106	26-27	0.39420109389332936
1106	28-29	0.37837749435083623
1106	30-31	0.47739708382378154
1106	32-33	0.37513302308975227
1106	34-35	0.3729980773883881
1106	36-37	0.3815950963625525
1106	38-39	0.38103405216428143
1106	40-41	0.4108484254319862
1106	42-43	0.39238021071878393
1106	44-45	0.33572370427425113
1106	46-47	0.3720303412616417
1106	48-49	0.26743449676015274
1106	50-51	0.3631161247973296
1106	52-53	0.3562081858725392
1106	54-55	0.35253785405436844
1106	56-57	0.3621395191679966
1106	58-59	0.2443955513430467
1106	60-61	0.32130539884907705
1106	62-63	0.3627926487150219
1106	64-65	0.29755706450654174
1106	66-67	0.30917365382588
1106	68-69	0.2982156277009125
1106	70-71	0.3012739355000207
1106	72-73	0.3112468032859397
1106	74-75	0.2830830434085385
1106	76-77	0.2644345482128969
1106	78-79	0.32457457234298204
1106	80-81	0.3245922470414797
1106	82-83	0.32974604247623773
1106	84-85	0.27132647046937564
1106	86-87	0.23618261017407605
1106	88-89	0.31000717462989513
1106	90-91	0.28455490307447917
1106	92-93	0.302631722728961
1106	94-95	0.3416887449379544
1106	96-97	0.324554453276658
1106	98-99	0.24881830291725038
1106	100	0.26273679978118025
1107	1	0.11495002805679633
1107	2	0.14862181475702485
1107	3	0.11625447784311405
1107	4	0.18453732654080568
1107	5	0.12724990425720506
1107	6	0.17585492193212815
1107	7	0.12282917974418694
1107	8	0.10879495395815297
1107	9	0.16341984941247034
1107	10-11	0.21241487569673012
1107	12-13	0.11520304347734367
1107	14-15	0.2773887021034014
1107	16-17	0.19094765450977036
1107	18-19	0.13139732064466614
1107	20-21	0.13341920107448146
1107	22-23	0.2943696535398459
1107	24-25	0.32083786003611436
1107	26-27	0.24772592633559753
1107	28-29	0.2796152531768499
1107	30-31	0.3740885075991187
1107	32-33	0.35025636953927375
1107	34-35	0.2855867231832292
1107	36-37	0.2877390190633662
1107	38-39	0.28841949332703365
1107	40-41	0.26023494797981783
1107	42-43	0.27272718754548464
1107	44-45	0.26131482685337204
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2216	88-89	-0.8513523524717499
2216	90-91	-0.9017443564937153
2216	92-93	-0.8563952337743963
2216	94-95	-0.8986286359851263
2216	96-97	-0.9067817791002888
2216	98-99	-0.8694831425624123
2216	100	-0.5320744715829093
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	15.0
3	21.0
4	46.0
5	33.0
6	57.0
7	324.0
8	804.0
9	1194.0
10	1461.0
11	2185.0
12	3450.0
13	5181.0
14	8392.0
15	12643.0
16	15892.0
17	18425.0
18	20536.0
19	22575.0
20	26501.0
21	29776.0
22	34214.0
23	40307.0
24	47245.0
25	54786.0
26	61977.0
27	67834.0
28	77912.0
29	94981.0
30	111809.0
31	130579.0
32	163515.0
33	214886.0
34	321021.0
35	463973.0
36	747188.0
37	1333849.0
38	1169046.0
39	514233.0
40	582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28738083226816	21.60762054497605	11.461612025663166	24.643386597092622
2	15.607990564722918	21.00455867687527	40.47432098879999	22.91312976960182
3	17.725801764527258	29.103426373803508	29.388492095475367	23.782279766193863
4	11.504180077681202	17.533985310997195	42.13881257163067	28.823022039690933
5	12.2905507942517	39.50843502024573	35.93986580059874	12.26114838490383
6	27.43064678685857	40.29780188527668	19.584538016137664	12.687013311727089
7	24.94169506018142	32.742287698190395	25.84474286370219	16.471274377925987
8	25.283944015236017	35.05240860306332	21.27795986128393	18.38568752041673
9	26.86825050284264	14.681613186205142	22.2038641341097	36.24627217684252
10-11	24.926513295139376	26.228283918328632	28.50673262971224	20.338470156819753
12-13	25.407193774584115	24.639181006333175	28.090985472901192	21.86263974618152
14-15	22.80248298677382	25.587844059989933	25.682200169328922	25.927472783907323
16-17	21.612115235264568	27.751356171190018	27.0786512702777	23.55787732326772
18-19	20.919719141105382	28.83495763754919	28.618009512552362	21.627313708793068
20-21	22.673443070125927	27.819635308359036	27.212812571447326	22.29410905006771
22-23	23.29289409016085	27.633848470505107	26.719791950649107	22.353465488684936
24-25	22.748361745440384	27.655439896985577	27.8055761786974	21.79062217887663
26-27	22.452645813895362	28.01908524943513	26.891123671512446	22.637145265157063
28-29	21.703770856634343	28.789864761119677	27.00519304858906	22.501171333656917
30-31	22.566608920576588	28.258897815541197	27.03455296672901	22.13994029715321
32-33	23.089318620148276	28.591382603015695	25.95441485772086	22.364883919115176
34-35	22.905621800171396	27.96314031155524	27.461658765027906	21.66957912324546
36-37	22.631555340397462	27.780621920313486	27.173032304475512	22.414790434813543
38-39	22.901501781206342	27.639983227618064	27.751358030207474	21.707156960968124
40-41	22.267202407655265	26.883444543151864	28.382601071590813	22.466751977602055
42-43	22.279431257140327	27.290196262087807	27.469130365477394	22.96124211529447
44-45	22.120511746318197	28.65080725725887	27.40334933883321	21.82533165758972
46-47	22.197967814433326	27.946809573843602	27.035582109940414	22.819640501782658
48-49	23.02705055052939	27.17484116270652	27.448713678311815	22.34939460845228
50-51	22.73516578997957	27.158933112287475	26.944853658664435	23.161047439068522
52-53	21.741863709862123	28.13780698411007	26.98313894354453	23.137190362483278
54-55	22.048510853902677	28.037707480860615	26.83114062225009	23.082641042986623
56-57	23.242780093926925	27.128376648129453	26.702859433063058	22.925983824880564
58-59	22.00308982579728	27.373118721804136	27.952774716157275	22.671016736241313
60-61	22.147392402487498	28.33979200266184	26.787949752308826	22.724865842541835
62-63	22.680775838312798	27.410183395572517	26.36357004273464	23.545470723380042
64-65	21.483332885074326	27.65528372346332	27.025275019791422	23.836108371670935
66-67	22.52511816966368	26.895876901956495	26.923898882669704	23.655106045710113
68-69	21.614703402202007	27.240179524716957	27.199829940594345	23.94528713248669
70-71	21.75403972808868	26.803734209366763	27.43102886497166	24.011197197572898
72-73	22.43837881580985	26.915322459379055	28.02674557624436	22.619553148566734
74-75	22.44972962252846	27.317933333270318	27.36527119507865	22.867065849122575
76-77	22.013013929575678	27.160077554949936	27.389349977194776	23.43755853827961
78-79	21.80522804534939	27.1726250371	27.361111087241845	23.661035830308762
80-81	21.401078956026737	27.43453169694333	28.146706604577815	23.017682742452113
82-83	22.316293984616127	26.12026299689985	29.189296246142	22.37414677234202
84-85	22.76116414755493	26.893406772815936	27.4681509232924	22.877278156336732
86-87	21.120403208635	27.60873446856059	28.094958465681707	23.1759038571227
88-89	22.373637530740975	26.21323359771795	27.80516084894194	23.60796802259913
90-91	21.965291643479986	26.085815525561145	28.23716718161436	23.711725649344512
92-93	22.405440637448283	26.436528373973967	27.926264366744487	23.231766621833266
94-95	20.950252877197745	26.59823028924065	28.57892982163694	23.87258701192467
96-97	21.407464144499865	26.379046460919152	28.0808852239044	24.13260417067658
98-99	20.708456208368563	27.324340255580072	27.897101180287752	24.070102355763613
100	21.72868289309396	26.398494319498468	27.013295479977874	24.859527307429698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	785.0
1	604.5
2	509.0
3	582.5
4	532.5
5	482.0
6	458.0
7	457.0
8	506.0
9	507.5
10	448.5
11	405.5
12	437.0
13	490.0
14	507.0
15	700.0
16	867.5
17	929.5
18	1216.5
19	1557.0
20	1857.0
21	2301.5
22	3081.5
23	4370.0
24	5950.0
25	7752.0
26	11235.0
27	14997.5
28	20038.0
29	33927.0
30	54973.0
31	74841.0
32	100224.0
33	124244.0
34	145568.0
35	167652.0
36	185601.5
37	219714.0
38	226936.5
39	200521.5
40	180110.0
41	161347.0
42	156059.0
43	164933.5
44	178388.5
45	211015.0
46	243016.0
47	262614.5
48	273168.5
49	261709.0
50	243212.0
51	243248.0
52	244455.0
53	233663.0
54	220082.5
55	188802.5
56	165491.5
57	133364.0
58	99182.5
59	94055.0
60	76617.5
61	50848.0
62	36555.5
63	29922.0
64	21899.0
65	9871.0
66	4651.0
67	3032.0
68	2164.0
69	1608.0
70	1259.5
71	1013.5
72	833.5
73	730.5
74	589.5
75	445.5
76	305.5
77	201.0
78	137.0
79	97.0
80	63.5
81	46.0
82	32.0
83	20.5
84	21.0
85	20.0
86	17.0
87	14.0
88	14.0
89	14.5
90	11.5
91	9.0
92	7.5
93	7.0
94	10.5
95	10.0
96	6.0
97	6.5
98	8.5
99	6.5
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05991976216305014
2	0.007492118240060185
3	0.0075608532697855075
4	0.009244861498055916
5	0.0034023839714034778
6	0.0024744610701116204
7	7.732690844098814E-4
8	0.001374700594506456
9	5.842477526652438E-4
10-11	4.983289655085902E-4
12-13	2.491644827542951E-4
14-15	1.031025445879842E-4
16-17	5.584721165182477E-4
18-19	0.0014692112603787747
20-21	6.787584185375626E-4
22-23	0.0010396173245955072
24-25	0.0011942711414774834
26-27	0.003273505790668498
28-29	0.003909304815627734
30-31	0.005086392199673887
32-33	0.006186152675279051
34-35	0.0032992814268154942
36-37	0.00811073350758809
38-39	0.006168968917847721
40-41	0.005902620677662095
42-43	0.014202375516994822
44-45	0.01952934032070734
46-47	0.017759413305280275
48-49	0.02115320539796809
50-51	0.018017169666750238
52-53	0.02502814269873316
54-55	0.018575641783268485
56-57	0.015173257811865005
58-59	0.017149389916468038
60-61	0.015688770534804927
62-63	0.010885910332747998
64-65	0.011006196634767313
66-67	0.013789965338642884
68-69	0.0052582297739871935
70-71	0.008471592413646034
72-73	0.008041998477862767
74-75	0.010989012877335983
76-77	0.010430540760817734
78-79	0.01230357032083278
80-81	0.010026722461181463
82-83	0.006340806492161027
84-85	0.010293070701367087
86-87	0.009038656408879948
88-89	0.007981855326853108
90-91	0.012630061712028063
92-93	0.008566103079518354
94-95	0.007199994363727563
96-97	0.009227677740624584
98-99	0.00909020768117394
100	0.2628427536696344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5819449.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.91793828436236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61438675393511	48.52508602887077
2	15.686144168135584	20.67996566389544
3	5.520282552707977	10.916569338649404
4	2.3291110425449535	6.141207918396205
5	1.1816085233306484	3.894459885859311
6	0.6052565709839687	2.3938359175395623
7	0.35094231880995114	1.6193375878879763
8	0.2176263540791683	1.1476384461793125
9	0.13734349263006368	0.8148059883852582
>10	0.352844910894533	3.20407140462566
>50	0.002774328157510266	0.12240632201456592
>100	0.0014178028513462102	0.17159196542304428
>500	3.731159626811032E-5	0.013256723865497717
>1k	2.238693429704817E-4	0.35576680840801667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.7183757431330697E-5	0.0
6	0.0	0.0	0.0	1.7183757431330697E-5	0.0
7	0.0	0.0	0.0	1.7183757431330697E-5	0.0
8	0.0	0.0	0.0	1.7183757431330697E-5	0.0
9	0.0	0.0	0.0	3.4367514862661394E-5	0.0
10-11	0.0	0.0	0.0	3.4367514862661394E-5	0.0
12-13	0.0	0.0	0.0	3.4367514862661394E-5	0.0
14-15	0.0	0.0	0.0	3.4367514862661394E-5	0.0
16-17	0.0	0.0	0.0	3.4367514862661394E-5	0.0
18-19	0.0	0.0	0.0	4.295939357832674E-5	0.0
20-21	0.0	0.0	0.0	6.873502972532279E-5	0.0
22-23	0.0	0.0	0.0	3.866345422049407E-4	0.0
24-25	0.0	0.0	0.0	7.217178121158893E-4	0.0
26-27	0.0	0.0	0.0	0.001598089441113755	0.0
28-29	0.0	0.0	0.0	0.00616896891784772	0.0
30-31	0.0	0.0	0.0	0.022003801390818957	0.0
32-33	0.0	0.0	0.0	0.05058898187783757	0.0
34-35	0.0	0.0	0.0	0.09318751655010638	0.0
36-37	0.0	0.0	0.0	0.15852875418274137	0.0
38-39	0.0	0.0	0.0	0.26210385210008713	0.0
40-41	0.0	0.0	0.0	0.3828369318126166	0.0
42-43	0.0	0.0	0.0	0.5077628483383908	0.0
44-45	0.0	0.0	0.0	0.6437894721648045	0.0
46-47	0.0	0.0	0.0	0.8090628511393433	0.0
48-49	0.0	0.0	0.0	1.0039782116829274	0.0
50-51	0.0	0.0	0.0	1.2191274466019033	0.0
52-53	0.0	0.0	0.0	1.4349210724245542	0.0
54-55	0.0	0.0	0.0	1.6513762729083115	0.0
56-57	0.0	0.0	0.0	1.9096309633437805	0.0
58-59	0.0	0.0	0.0	2.2249357284512676	0.0
60-61	0.0	0.0	0.0	2.5718500153536876	0.0
62-63	0.0	0.0	0.0	2.9308187080941854	0.0
64-65	0.0	0.0	0.0	3.2960938398119826	0.0
66-67	0.0	0.0	0.0	3.690486848497169	0.0
68-69	0.0	0.0	0.0	4.127933761426554	0.0
70-71	0.0	0.0	0.0	4.622035522607037	0.0
72-73	0.0	0.0	0.0	5.157421261016292	0.0
74-75	0.0	0.0	0.0	5.666412748011023	0.0
76-77	0.0	0.0	0.0	6.229447152127289	0.0
78-79	0.0	0.0	0.0	6.824142629310781	0.0
80-81	0.0	0.0	0.0	7.488518242878321	0.0
82-83	0.0	0.0	0.0	8.203938207895627	0.0
84-85	0.0	0.0	0.0	8.899966302651677	0.0
86-87	0.0	0.0	0.0	9.619725166420395	0.0
88	0.0	0.0	0.0	10.177939526577173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5155	0.0	39.75393	94
GTATCAA	12200	0.0	34.99398	1
CAACGCA	11000	0.0	34.732616	5
ATCAACG	11850	0.0	32.441486	3
CTGTGCG	7145	0.0	30.64818	9
TAGTACT	7330	0.0	30.005968	4
TACCCCG	425	0.0	29.854822	5
TATCAAC	14235	0.0	29.74682	2
ACTGTGC	7675	0.0	28.837893	8
GTACTAG	6265	0.0	28.743858	1
TATTCTC	3765	0.0	28.707972	5
GGTATCA	7395	0.0	28.484407	1
ACGCAGA	13315	0.0	28.375002	7
AACGCAG	14475	0.0	28.276411	6
CGCAGAG	13315	0.0	28.057371	8
TAGGCAT	7050	0.0	27.596302	5
TACATGG	25500	0.0	27.442757	2
CGTTAAC	1305	0.0	27.38233	1
GTACATG	25840	0.0	27.312128	1
ACATGGG	25215	0.0	26.78327	3
>>END_MODULE