FastQCFastQC Report
Mon 16 Jan 2017
ERR1146678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5819449
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160640.27603987937689634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108670.1867358920062707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83680.1437936821853753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197500.044.559591
GGTATCA102300.043.404111
GGGCACC51600.038.00155694
CAACGCA241350.035.4612245
ATCAACG242650.035.298843
TATCAAC259950.033.8003772
AACGCAG281700.031.5316986
CTGTGCG67900.030.2533639
ACTGTGC72250.029.59798
ACGCAGA290350.029.4433357
CGCAGAG291700.029.2272648
TAGTACT71500.029.0768284
CAGTACG7550.028.639999
TCAACGC309300.028.1942544
GTACTAG62700.027.981811
GCAGAGT310450.027.2093649
TAGGCAT70950.027.0270615
GTGGTAT21600.027.0024051
TACTGTG89550.026.975767
TATTCTC40550.026.7739355
Produced by FastQC (version 0.11.5)