FastQCFastQC Report
Mon 16 Jan 2017
ERR1146677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625140
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC65610.14185516546526158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC53450.041.43166494
GTACTAG54200.037.5632551
TAGGCAT68900.033.556835
GCATCGT55900.033.540388
CTGTGCG71050.032.4066549
CTAGGCA76050.031.2082564
TATTCTC26800.030.8612085
TAGTACT75550.030.8549294
ACTGTGC80700.029.754618
GTACTGT82300.028.8338816
CAACGCA98950.027.877735
CAGTACG7250.027.8697249
TCTAACG28300.027.734572
CTAGTAC88800.027.5744443
TACTGTG88800.027.305097
CTAACGC28850.027.042343
CGTTAAC10100.027.0010831
GTATCAA115750.026.7694231
TAACGCC29300.026.6267284
AGGGCTA58100.026.4488435
Produced by FastQC (version 0.11.5)