##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146677_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4625140
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446045741318102	31.0	31.0	34.0	28.0	34.0
2	31.51137111525273	33.0	31.0	34.0	28.0	34.0
3	31.64569504923094	33.0	31.0	34.0	28.0	34.0
4	35.069813238085764	37.0	35.0	37.0	32.0	37.0
5	35.220320682184756	37.0	35.0	37.0	32.0	37.0
6	35.28429215115651	37.0	35.0	37.0	32.0	37.0
7	35.23210756863576	37.0	35.0	37.0	32.0	37.0
8	35.17806271810151	37.0	35.0	37.0	32.0	37.0
9	36.69341252372901	39.0	37.0	39.0	32.0	39.0
10-11	36.534640897356624	39.0	37.0	39.0	32.0	39.0
12-13	36.46639399023597	39.0	37.0	39.0	32.0	39.0
14-15	37.65335416873867	40.0	38.0	41.0	31.5	41.0
16-17	37.69077854940607	40.0	38.0	41.0	31.5	41.0
18-19	37.66291214968628	40.0	37.0	41.0	31.5	41.0
20-21	37.73347120303386	40.0	37.5	41.0	31.5	41.0
22-23	37.67628709617439	40.0	37.0	41.0	31.0	41.0
24-25	37.635986802561646	40.0	37.0	41.0	31.0	41.0
26-27	37.67854973038654	40.0	37.0	41.0	31.0	41.0
28-29	37.67531296349948	40.0	37.0	41.0	31.0	41.0
30-31	37.54739326809567	40.0	37.0	41.0	31.0	41.0
32-33	37.543778891017354	40.0	37.0	41.0	31.0	41.0
34-35	37.47958980701125	40.0	37.0	41.0	31.0	41.0
36-37	37.362724804005936	40.0	36.0	41.0	30.0	41.0
38-39	37.256408454662996	40.0	36.0	41.0	30.0	41.0
40-41	37.17113341433989	40.0	36.0	41.0	30.0	41.0
42-43	37.0077920884557	40.0	35.5	41.0	30.0	41.0
44-45	36.83606928655132	39.0	35.0	41.0	30.0	41.0
46-47	36.82831471047363	39.0	35.0	41.0	30.0	41.0
48-49	36.68923265025491	39.0	35.0	41.0	30.0	41.0
50-51	36.50434765217918	39.0	35.0	41.0	29.0	41.0
52-53	36.285033101700705	39.0	35.0	41.0	29.0	41.0
54-55	36.07034522198247	38.0	35.0	40.0	29.0	41.0
56-57	35.82007863545752	38.0	34.0	40.0	28.5	41.0
58-59	35.51728693617923	37.0	34.0	40.0	28.0	41.0
60-61	35.26265064408861	37.0	34.0	40.0	28.0	41.0
62-63	34.95214858361044	36.0	34.0	40.0	27.5	41.0
64-65	34.79920942933619	36.0	34.0	39.0	28.0	41.0
66-67	34.54418266257886	35.0	33.0	39.0	27.5	41.0
68-69	34.416849003489624	35.0	33.0	39.0	27.0	41.0
70-71	34.3353436868938	35.0	33.0	38.5	28.0	41.0
72-73	34.113969739294376	35.0	33.0	37.5	27.0	40.5
74-75	33.86751092939889	35.0	33.0	37.0	27.0	40.0
76-77	33.605931820442194	35.0	33.0	36.5	27.0	39.0
78-79	33.34834534738408	35.0	33.0	36.0	26.0	39.0
80-81	33.16099069433574	35.0	33.0	36.0	26.0	39.0
82-83	32.898346104117934	35.0	33.0	35.5	26.0	37.0
84-85	32.72041570633537	35.0	33.0	35.0	26.0	37.0
86-87	32.549275265181166	35.0	33.0	35.0	25.5	36.5
88-89	32.41986653376979	35.0	33.0	35.0	25.0	36.0
90-91	32.26856138408783	35.0	32.0	35.0	25.0	36.0
92-93	32.1108620495812	35.0	32.0	35.0	25.0	36.0
94-95	32.04170998067086	35.0	32.0	35.0	25.0	36.0
96-97	31.92859243179666	35.0	32.0	35.0	25.0	35.0
98-99	31.839302053559457	35.0	32.0	35.0	25.0	35.0
100	30.887535944857884	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43086697246361894
1101	2	0.5100663222788562
1101	3	0.5426545034447159
1101	4	0.6051476311137947
1101	5	0.4944026193696729
1101	6	0.509851134633692
1101	7	0.4918340953418152
1101	8	0.4566714075867466
1101	9	0.7311392406480479
1101	10-11	0.7378221156830804
1101	12-13	0.6662048155938507
1101	14-15	0.7826424612462759
1101	16-17	0.7937410900677122
1101	18-19	0.7567050758829552
1101	20-21	0.7998994875437972
1101	22-23	0.79052980107042
1101	24-25	0.8001743236996006
1101	26-27	0.7516650097935127
1101	28-29	0.6807443297229767
1101	30-31	0.7461519686276645
1101	32-33	0.7058874917489604
1101	34-35	0.7417181692572967
1101	36-37	0.7653459496034714
1101	38-39	0.7617385805843355
1101	40-41	0.6886387609384883
1101	42-43	0.6636779043045706
1101	44-45	0.6692685827487708
1101	46-47	0.6806047423792094
1101	48-49	0.6321024416103782
1101	50-51	0.5872521298571698
1101	52-53	0.6274930329707402
1101	54-55	0.5966605111109686
1101	56-57	0.5455077883046471
1101	58-59	0.5358272727996223
1101	60-61	0.5756511964117479
1101	62-63	0.5439158690525971
1101	64-65	0.5921486877270823
1101	66-67	0.5739009983841257
1101	68-69	0.62019561537398
1101	70-71	0.6042949501879704
1101	72-73	0.5574135402923233
1101	74-75	0.477940090261022
1101	76-77	0.49007391360984087
1101	78-79	0.5662032613147758
1101	80-81	0.49140875311623944
1101	82-83	0.5029267596428255
1101	84-85	0.5635221069235143
1101	86-87	0.5506370086009511
1101	88-89	0.5304629986332117
1101	90-91	0.49073653379997495
1101	92-93	0.4735637569870761
1101	94-95	0.47226261676807724
1101	96-97	0.5437799512065453
1101	98-99	0.5310138112360931
1101	100	0.6117819770808239
1102	1	0.3161880906239283
1102	2	0.3912113416415579
1102	3	0.38811863213126685
1102	4	0.4551813562058413
1102	5	0.3344280555244623
1102	6	0.35589400368599655
1102	7	0.34722592480223113
1102	8	0.35361945407557016
1102	9	0.4702160835246971
1102	10-11	0.5813237300592746
1102	12-13	0.5225715417955215
1102	14-15	0.671243719356788
1102	16-17	0.659078681585477
1102	18-19	0.7243978633523582
1102	20-21	0.6115996740884384
1102	22-23	0.6592391683396528
1102	24-25	0.6638482372194261
1102	26-27	0.5738373943844337
1102	28-29	0.6282490946471242
1102	30-31	0.6776303241047117
1102	32-33	0.6416340860739211
1102	34-35	0.6546933128055343
1102	36-37	0.7092541812305484
1102	38-39	0.7272468008062063
1102	40-41	0.5975377157586053
1102	42-43	0.6464629798901242
1102	44-45	0.6045888574379745
1102	46-47	0.5633497668394156
1102	48-49	0.5020591605407745
1102	50-51	0.5392083823113722
1102	52-53	0.5273659301262228
1102	54-55	0.502455744380697
1102	56-57	0.47991560145085543
1102	58-59	0.44694075184381177
1102	60-61	0.44326029229414843
1102	62-63	0.5039586715440265
1102	64-65	0.5247517019077534
1102	66-67	0.5478555961680485
1102	68-69	0.46490894017296824
1102	70-71	0.5101081301391375
1102	72-73	0.5091666491480424
1102	74-75	0.4921587440096218
1102	76-77	0.43701064199995443
1102	78-79	0.45288117609650413
1102	80-81	0.5086961289483583
1102	82-83	0.48535088460344156
1102	84-85	0.45844632700382704
1102	86-87	0.48487667761555286
1102	88-89	0.476815164873301
1102	90-91	0.4652615289202231
1102	92-93	0.4251743008893314
1102	94-95	0.534564395327763
1102	96-97	0.4985856003359501
1102	98-99	0.4168474068553252
1102	100	0.48241899671600663
1103	1	0.30316482812913037
1103	2	0.34607284259896076
1103	3	0.35832094178161356
1103	4	0.4827644693577682
1103	5	0.31120784473675656
1103	6	0.3270757724868929
1103	7	0.35588360765380145
1103	8	0.3481148409538619
1103	9	0.3754707067818188
1103	10-11	0.46661035657468375
1103	12-13	0.43854677636978323
1103	14-15	0.6172103857883044
1103	16-17	0.5844766208625742
1103	18-19	0.5659827122309977
1103	20-21	0.5343550752438517
1103	22-23	0.6142953244792437
1103	24-25	0.601245995971432
1103	26-27	0.5601217365434081
1103	28-29	0.5510514544263359
1103	30-31	0.5277684207055344
1103	32-33	0.4778251739305759
1103	34-35	0.553315912253062
1103	36-37	0.5430194205788581
1103	38-39	0.6204400909160768
1103	40-41	0.5422972905794978
1103	42-43	0.5538467836440404
1103	44-45	0.43118648968641793
1103	46-47	0.4680429901872927
1103	48-49	0.4265795260072096
1103	50-51	0.43618853483468456
1103	52-53	0.4053531041749352
1103	54-55	0.4105067430610063
1103	56-57	0.40972484659671693
1103	58-59	0.45465216530518404
1103	60-61	0.4448928162874566
1103	62-63	0.3785639747492766
1103	64-65	0.4052025038463114
1103	66-67	0.3564642933131452
1103	68-69	0.3623982006719686
1103	70-71	0.3507755647457529
1103	72-73	0.3724218404982693
1103	74-75	0.36000859234388827
1103	76-77	0.33328333417058076
1103	78-79	0.316077722287595
1103	80-81	0.28617629434810254
1103	82-83	0.35031755842934587
1103	84-85	0.3321009411837039
1103	86-87	0.2994327800820784
1103	88-89	0.38728094172575567
1103	90-91	0.38044320524875275
1103	92-93	0.4048804835424846
1103	94-95	0.35656256848855605
1103	96-97	0.34462525024304824
1103	98-99	0.35286394462021065
1103	100	0.4773076461324415
1104	1	0.25865970084195666
1104	2	0.302953538181562
1104	3	0.3192394730434138
1104	4	0.38153753555796044
1104	5	0.35271664885447507
1104	6	0.29116155339347216
1104	7	0.28584318834018063
1104	8	0.3411135645832246
1104	9	0.3702026451543574
1104	10-11	0.5101444951009526
1104	12-13	0.36873438904492417
1104	14-15	0.49162687782938264
1104	16-17	0.5422102325358722
1104	18-19	0.43982734950450464
1104	20-21	0.3949646126291242
1104	22-23	0.48075154588699576
1104	24-25	0.47676740558514297
1104	26-27	0.4596381885872205
1104	28-29	0.3800829300959947
1104	30-31	0.42504266221052234
1104	32-33	0.46676439377096557
1104	34-35	0.49358584658250493
1104	36-37	0.3911869577015352
1104	38-39	0.4403304912374253
1104	40-41	0.4649603547100014
1104	42-43	0.49987101510486553
1104	44-45	0.43699798984747673
1104	46-47	0.4421367073560063
1104	48-49	0.4319544849431125
1104	50-51	0.3684969308196031
1104	52-53	0.33762040681395433
1104	54-55	0.47238612529481117
1104	56-57	0.45189366016804655
1104	58-59	0.4583851652005748
1104	60-61	0.40507172583271966
1104	62-63	0.4414915786105098
1104	64-65	0.4570738523017397
1104	66-67	0.4461098857309693
1104	68-69	0.4031261141979883
1104	70-71	0.4132920401154436
1104	72-73	0.4388569281852668
1104	74-75	0.3771190073363684
1104	76-77	0.3492486744004637
1104	78-79	0.4124595419204624
1104	80-81	0.35127962217189435
1104	82-83	0.3861795283183582
1104	84-85	0.3999506970459308
1104	86-87	0.46489171454780376
1104	88-89	0.3338370072869452
1104	90-91	0.44073084930852247
1104	92-93	0.40724856805115905
1104	94-95	0.4904234309050679
1104	96-97	0.41596811603643147
1104	98-99	0.40339272841242035
1104	100	0.5440249848407674
1105	1	0.13124007586831254
1105	2	0.21964202490248041
1105	3	0.21954505864405505
1105	4	0.3038055159285591
1105	5	0.17997539515783956
1105	6	0.20801048444296555
1105	7	0.2894523245382459
1105	8	0.2538992476920825
1105	9	0.4079041792380309
1105	10-11	0.3640241194687732
1105	12-13	0.2371904461890182
1105	14-15	0.3845558996922236
1105	16-17	0.41880119157097084
1105	18-19	0.3724570286798752
1105	20-21	0.3461092864970965
1105	22-23	0.4062970275856159
1105	24-25	0.3846321937389092
1105	26-27	0.42965389565638645
1105	28-29	0.45944142836403756
1105	30-31	0.4913954720625
1105	32-33	0.444730613443852
1105	34-35	0.46357640114811716
1105	36-37	0.397298229756224
1105	38-39	0.412283643140988
1105	40-41	0.40542784230495954
1105	42-43	0.39693132865539127
1105	44-45	0.38393483802367
1105	46-47	0.4549389291486321
1105	48-49	0.28715370306813526
1105	50-51	0.3244659147779032
1105	52-53	0.36714106805576563
1105	54-55	0.33304170494010776
1105	56-57	0.3543808767614891
1105	58-59	0.4417763408673423
1105	60-61	0.38936013539058223
1105	62-63	0.34485477068955106
1105	64-65	0.34370547171145915
1105	66-67	0.33647850067854534
1105	68-69	0.31789003656270864
1105	70-71	0.29404735322845
1105	72-73	0.25580285784744916
1105	74-75	0.2460460163249465
1105	76-77	0.24819315103986384
1105	78-79	0.32439638653954006
1105	80-81	0.26051384104933817
1105	82-83	0.2891424801315594
1105	84-85	0.21746566114740062
1105	86-87	0.23023387115846816
1105	88-89	0.21780829310651484
1105	90-91	0.18117902929134289
1105	92-93	0.242347266400202
1105	94-95	0.21116157741099784
1105	96-97	0.06769150904045063
1105	98-99	0.1213876138890555
1105	100	0.2563558672512478
1106	1	0.1558823646620624
1106	2	0.27126108779928515
1106	3	0.2558401094096574
1106	4	0.3571891983226223
1106	5	0.2683308477472295
1106	6	0.2359971458554142
1106	7	0.2806858765261495
1106	8	0.22441306074804146
1106	9	0.31948209958235907
1106	10-11	0.4054099992579623
1106	12-13	0.2720649978270302
1106	14-15	0.4367627590058518
1106	16-17	0.3494595421568292
1106	18-19	0.35573177514248044
1106	20-21	0.39234696901438326
1106	22-23	0.44749086294488194
1106	24-25	0.46140568651222935
1106	26-27	0.49557107079277785
1106	28-29	0.4599346217088467
1106	30-31	0.46609289916117547
1106	32-33	0.430486380201458
1106	34-35	0.41349144631077195
1106	36-37	0.42241473509588445
1106	38-39	0.46769379385881393
1106	40-41	0.48741306733163015
1106	42-43	0.40929434987801727
1106	44-45	0.4052811891116974
1106	46-47	0.3624660924165255
1106	48-49	0.31423706304067167
1106	50-51	0.3039798355531076
1106	52-53	0.28311371085447945
1106	54-55	0.3310483370358668
1106	56-57	0.3623986469498348
1106	58-59	0.3845061344997376
1106	60-61	0.326980781915303
1106	62-63	0.3512615181766563
1106	64-65	0.34166911297847236
1106	66-67	0.3839626847420661
1106	68-69	0.37943197945367757
1106	70-71	0.34747857933308524
1106	72-73	0.3094190762586848
1106	74-75	0.3363558564006226
1106	76-77	0.3319672323942413
1106	78-79	0.30479986292636596
1106	80-81	0.32038166023444603
1106	82-83	0.34060483671093067
1106	84-85	0.27776746683970543
1106	86-87	0.24536024947078516
1106	88-89	0.3377701128333115
1106	90-91	0.2993429063007582
1106	92-93	0.3342451807153566
1106	94-95	0.34835881561350845
1106	96-97	0.3268907036259705
1106	98-99	0.2909250372214274
1106	100	0.30404294516520025
1107	1	0.10723454002835808
1107	2	0.14111325854000611
1107	3	0.09091978388201127
1107	4	0.1530719163409131
1107	5	0.14884538431117278
1107	6	0.17692879237000625
1107	7	0.14222195256894565
1107	8	0.1288482804164559
1107	9	0.18251995807927557
1107	10-11	0.17961021774585362
1107	12-13	0.13436757137463218
1107	14-15	0.30447161062112826
1107	16-17	0.22883638911756776
1107	18-19	0.1738378930044675
1107	20-21	0.1865073955162586
1107	22-23	0.26756473936327296
1107	24-25	0.34799956209399596
1107	26-27	0.25299580770833074
1107	28-29	0.2450988335375186
1107	30-31	0.29902481999229735
1107	32-33	0.2657392220121295
1107	34-35	0.23555522369767345
1107	36-37	0.30591900007438966
1107	38-39	0.32337133155822784
1107	40-41	0.27065957733754686
1107	42-43	0.3370627949497518
1107	44-45	0.3551424420625082
1107	46-47	0.30516318904552975
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2216	96-97	-1.0987536596371115
2216	98-99	-1.0700182161924765
2216	100	-0.7897760098763982
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7.0
3	27.0
4	36.0
5	31.0
6	48.0
7	123.0
8	440.0
9	728.0
10	1059.0
11	1884.0
12	3333.0
13	5202.0
14	7442.0
15	10305.0
16	12690.0
17	14862.0
18	16742.0
19	19035.0
20	22341.0
21	25347.0
22	29296.0
23	34450.0
24	40493.0
25	46686.0
26	52701.0
27	58092.0
28	66818.0
29	80817.0
30	94377.0
31	109812.0
32	136424.0
33	177633.0
34	260325.0
35	374614.0
36	601109.0
37	1050854.0
38	905409.0
39	363011.0
40	537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83589283411891	21.047347375325472	12.046556464055506	24.070203326500113
2	15.603464548745706	19.906278927007882	40.48558344416727	24.004673080079147
3	18.42987981881033	26.96131502408662	28.997698694874792	25.61110646222826
4	11.881803411333314	16.627190249231194	40.889445417239855	30.601560922195638
5	12.83063898166999	38.82895594882058	35.478176789368355	12.862228280141078
6	29.364131279666044	39.14623633548517	18.259093406373626	13.230538978475154
7	26.594191179268616	31.993031940470964	24.089577228966093	17.323199651294328
8	26.141792789000302	35.048895444461095	19.985068343786143	18.824243422752463
9	26.131567436976106	14.944765649948726	21.690484833769393	37.23318207930577
10-11	25.521259617456288	25.97875059849818	27.846497338392517	20.65349244565301
12-13	25.699731683217554	23.909317143518127	27.716593911954906	22.67435726130941
14-15	23.085810134643623	25.17918664220756	25.149782098275825	26.585221124872987
16-17	21.954957852376562	27.644116764727343	26.481528827677163	23.919396555218928
18-19	21.231816898791056	28.653314581907452	28.04343198660431	22.071436532697177
20-21	23.136736375474936	27.697029921750833	26.92480827969662	22.24142542307761
22-23	23.714531454996276	27.303612215475436	26.558382507215008	22.42347382231328
24-25	23.10450764289813	27.29200458889558	27.49738490896802	22.106102859238266
26-27	22.96912925987284	27.527069411597328	26.379200520998875	23.12460080753096
28-29	21.950280014382887	28.216230217855752	26.925738079685775	22.90775168807559
30-31	22.83020316202799	27.752741692757432	26.952850668557886	22.46420447665669
32-33	23.35308249782641	27.96933167590555	25.846121711965196	22.83146411430284
34-35	23.247952932003756	27.729778051461324	27.03534088934876	21.986928127186165
36-37	22.7990079871543	27.277466607030863	26.72943562106268	23.194089784752155
38-39	23.040136757222633	27.398905648154592	27.327076105152226	22.23388148947055
40-41	22.62389926064565	26.45481600900873	27.99000226169693	22.931282468648686
42-43	22.55646723114436	26.73407573481139	27.211440404181946	23.498016629862306
44-45	22.741559810564944	27.79593659014087	27.131157901725295	22.331345697568892
46-47	22.746125446492048	27.47944122740103	26.631172565020112	23.143260761086815
48-49	23.486342554572097	27.015102108209625	26.90208526456938	22.5964700726489
50-51	22.97497622313019	26.820114518228067	26.942999522948842	23.2619097356929
52-53	22.35074137848257	27.59066158530124	26.67444356064096	23.384153475575236
54-55	22.355908287334632	27.44240386226881	26.686125783239355	23.5155620671572
56-57	23.66914652446706	26.74179943458507	26.404025264729427	23.18502877621845
58-59	22.470468535271788	27.225810148768304	27.35747317705039	22.94624813890952
60-61	22.450168060784947	27.970283942553454	26.584602849016033	22.994945147645566
62-63	22.914693967134347	27.11709266885569	26.18328996007209	23.784923403937867
64-65	21.925642480521457	27.39867395410952	26.732256103045987	23.943427462323033
66-67	22.778357663673244	26.892242640404646	26.660378382574617	23.66902131334749
68-69	22.14518615577884	27.02505356884164	26.960241216547853	23.86951905883167
70-71	22.16572754735797	26.671910944720366	27.09040087373882	24.071960634182847
72-73	22.756377648792345	26.692589646672005	27.67388032003197	22.877152384503685
74-75	22.973947494520587	26.98787609869342	27.083117097667596	22.955059309118397
76-77	22.561997445847513	26.936016015132903	26.74881168769107	23.753174851328517
78-79	22.451588126551915	26.875522890183383	26.80934329209605	23.86354569116865
80-81	22.05371533944927	27.222184741811144	27.53079268042169	23.193307238317896
82-83	22.884706219663038	26.101812079571886	28.716797794164943	22.29668390660013
84-85	23.0921576288936	26.786307772181388	27.21023550315281	22.911299095772204
86-87	21.6624708831363	27.50868843428711	27.702247667812873	23.126593014763717
88-89	22.930245979330596	26.150803333838944	27.41741001178764	23.501540675042815
90-91	22.47136453375601	26.213544786366555	27.6118010340555	23.703289645821933
92-93	22.796071025303597	26.45959169834788	27.545236271703487	23.19910100464504
94-95	21.59590882868577	26.451666541255513	28.23987823874305	23.712546391315666
96-97	21.929692926046407	26.327323120887826	27.791271792840334	23.951712160225433
98-99	21.21948582555174	27.13786530787436	27.622340500292452	24.02030836628145
100	22.141804088390675	26.293926159591653	26.679544454924237	24.884725297093436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	613.0
1	414.5
2	232.0
3	271.5
4	272.0
5	241.0
6	230.0
7	230.0
8	256.5
9	260.0
10	234.5
11	223.0
12	235.5
13	256.0
14	285.0
15	433.0
16	559.5
17	637.0
18	884.0
19	1206.5
20	1558.0
21	2090.0
22	3005.5
23	4388.0
24	5964.5
25	7869.5
26	11089.5
27	14578.0
28	19228.5
29	29451.5
30	43502.0
31	55768.5
32	71404.0
33	86434.5
34	97030.0
35	111316.5
36	123666.0
37	144239.0
38	152147.0
39	139629.5
40	129582.5
41	119687.5
42	117725.5
43	124906.0
44	136234.5
45	164049.0
46	192717.5
47	213147.5
48	226134.0
49	220419.0
50	207202.5
51	211118.0
52	214574.5
53	202713.5
54	189472.5
55	161888.0
56	141319.0
57	115633.5
58	88243.0
59	86106.0
60	71741.5
61	47572.0
62	34621.5
63	29134.5
64	21280.0
65	9582.5
66	4972.5
67	3432.5
68	2421.5
69	1703.0
70	1169.5
71	806.5
72	556.0
73	365.0
74	246.5
75	167.5
76	101.0
77	62.0
78	42.5
79	34.0
80	26.0
81	23.0
82	24.5
83	22.5
84	18.0
85	11.5
86	11.0
87	10.5
88	9.0
89	9.0
90	8.0
91	4.5
92	2.0
93	3.5
94	5.0
95	5.0
96	6.0
97	5.0
98	2.5
99	3.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.056733417799245
2	0.0076322014036331876
3	0.008064620746615236
4	0.0094699836113069
5	0.0033728708752599926
6	0.002443169287848584
7	7.783548173676904E-4
8	0.0014486047989898684
9	6.270080473239729E-4
10-11	4.648507937057041E-4
12-13	2.1620967149102514E-4
14-15	9.72943521709613E-5
16-17	5.945765966003191E-4
18-19	0.0015026572168626246
20-21	9.297015874114082E-4
22-23	0.0012107741603497406
24-25	0.0013404999632443558
26-27	0.0038485321525402474
28-29	0.0043025724626714005
30-31	0.0056214514587666535
32-33	0.006551153046178061
34-35	0.0037079958660710816
36-37	0.00868081831036466
38-39	0.006529532079028959
40-41	0.006356564341836139
42-43	0.01487522539858253
44-45	0.020961527651054888
46-47	0.019404818016319506
48-49	0.02271282599013219
50-51	0.019415628499894057
52-53	0.025642467038835583
54-55	0.0197831849414288
56-57	0.016183293911103232
58-59	0.0180535075695006
60-61	0.01668057615553259
62-63	0.011999636767751894
64-65	0.012194225472093818
66-67	0.014421185088451378
68-69	0.005405241787275628
70-71	0.00901594330117575
72-73	0.008961890883302992
74-75	0.012021257734900996
76-77	0.011113177114638691
78-79	0.013037443190908816
80-81	0.010853725508849462
82-83	0.0068214151355418425
84-85	0.011480733556173435
86-87	0.0098915924707144
88-89	0.008248398967382609
90-91	0.013621209303934585
92-93	0.009124048136921262
94-95	0.0076105804364840855
96-97	0.009859161019990747
98-99	0.009621330381350619
100	0.2600786138365542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4625140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.94180961701308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47810155046135	51.28118805795023
2	14.747070399733166	20.038852990147195
3	5.046058860942458	10.28515111339183
4	2.1516044324541697	5.847355948836906
5	1.0662985568823036	3.622312677329662
6	0.5563634913993821	2.2680205446308106
7	0.3209568468603549	1.5264472289264137
8	0.20002455220882046	1.0872024035919976
9	0.11613543306195953	0.7101406334596517
>10	0.3155597378903608	2.9347355118985083
>50	0.001375039498706285	0.06410541841643046
>100	3.1667841891041735E-4	0.048632370511812045
>500	0.0	0.0
>1k	8.961345872523339E-5	0.08612184030566174
>5k	4.4806729362616694E-5	0.19973326060284433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6561	0.14185516546526158	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.0810483574551257E-5	0.0
22-23	0.0	0.0	0.0	1.0810483574551257E-4	0.0
24-25	0.0	0.0	0.0	2.3783063864012764E-4	0.0
26-27	0.0	0.0	0.0	5.837661130257679E-4	0.0
28-29	0.0	0.0	0.0	0.002356685419252174	0.0
30-31	0.0	0.0	0.0	0.00980510860211799	0.0
32-33	0.0	0.0	0.0	0.023383075971754368	0.0
34-35	0.0	0.0	0.0	0.04554456729958445	0.0
36-37	0.0	2.1620967149102513E-5	0.0	0.08314342917187373	0.0
38-39	0.0	2.1620967149102513E-5	0.0	0.13946604859528577	0.0
40-41	0.0	2.1620967149102513E-5	0.0	0.202577651703516	0.0
42-43	0.0	2.1620967149102513E-5	0.0	0.27058640387101796	0.0
44-45	0.0	2.1620967149102513E-5	0.0	0.3507893815106138	0.0
46-47	0.0	2.1620967149102513E-5	0.0	0.45164038277760243	0.0
48-49	0.0	2.1620967149102513E-5	0.0	0.5685773836035234	0.0
50-51	0.0	2.1620967149102513E-5	0.0	0.6956546180223734	0.0
52-53	0.0	2.1620967149102513E-5	0.0	0.8206778605620586	0.0
54-55	0.0	2.1620967149102513E-5	0.0	0.9595709535278931	0.0
56-57	0.0	2.1620967149102513E-5	0.0	1.132376533467095	0.0
58-59	0.0	2.1620967149102513E-5	0.0	1.340856709202316	0.0
60-61	0.0	2.1620967149102513E-5	0.0	1.559671707234808	0.0
62-63	0.0	2.1620967149102513E-5	0.0	1.7906809307393938	0.0
64-65	0.0	2.1620967149102513E-5	0.0	2.033073593447982	0.0
66-67	0.0	2.1620967149102513E-5	0.0	2.3084382310589517	0.0
68-69	0.0	2.1620967149102513E-5	0.0	2.6184396580427833	0.0
70-71	0.0	2.1620967149102513E-5	0.0	2.967812866205131	0.0
72-73	0.0	2.1620967149102513E-5	0.0	3.335650380312812	0.0
74-75	0.0	2.1620967149102513E-5	0.0	3.687737019852372	0.0
76-77	0.0	2.1620967149102513E-5	0.0	4.095897637693129	0.0
78-79	0.0	2.1620967149102513E-5	0.0	4.538370730399512	0.0
80-81	0.0	2.1620967149102513E-5	0.0	5.0226803945394085	0.0
82-83	0.0	2.1620967149102513E-5	0.0	5.5307406910925945	0.0
84-85	0.0	2.1620967149102513E-5	0.0	6.026152721863554	0.0
86-87	0.0	2.1620967149102513E-5	0.0	6.552936775967863	0.0
88	0.0	2.1620967149102513E-5	0.0	6.976545574836654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5345	0.0	41.431664	94
GTACTAG	5420	0.0	37.563255	1
TAGGCAT	6890	0.0	33.55683	5
GCATCGT	5590	0.0	33.54038	8
CTGTGCG	7105	0.0	32.406654	9
CTAGGCA	7605	0.0	31.208256	4
TATTCTC	2680	0.0	30.861208	5
TAGTACT	7555	0.0	30.854929	4
ACTGTGC	8070	0.0	29.75461	8
GTACTGT	8230	0.0	28.833881	6
CAACGCA	9895	0.0	27.87773	5
CAGTACG	725	0.0	27.869724	9
TCTAACG	2830	0.0	27.73457	2
CTAGTAC	8880	0.0	27.574444	3
TACTGTG	8880	0.0	27.30509	7
CTAACGC	2885	0.0	27.04234	3
CGTTAAC	1010	0.0	27.001083	1
GTATCAA	11575	0.0	26.769423	1
TAACGCC	2930	0.0	26.626728	4
AGGGCTA	5810	0.0	26.448843	5
>>END_MODULE