FastQCFastQC Report
Mon 16 Jan 2017
ERR1146677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625140
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125600.2715593473927276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105370.2278201308500932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.14823335077424682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC51100.041.6874994
GTATCAA191750.036.3612331
GGTATCA117400.034.3029481
GTACTAG54650.033.832041
CTGTGCG68400.033.191869
TAGTACT72900.031.685534
CAACGCA214700.031.413295
ACTGTGC77600.031.0692278
ATCAACG220200.030.593753
GTACTGT78150.029.8880466
TACCCCG4150.029.4454945
TACTGTG86950.029.133597
TATCAAC241250.028.920092
CTAACGC22950.028.9030323
TCTAACG23750.028.7230242
AACGCAG246050.028.3452766
CGCCTAC24000.028.0026827
TATTCTC30400.027.8286765
TAACGCC24300.027.6845054
CTAGTAC86400.027.5514053
Produced by FastQC (version 0.11.5)