##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4625140
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12977639595774	33.0	31.0	34.0	30.0	34.0
2	32.188791042000894	33.0	31.0	34.0	30.0	34.0
3	32.34079444081693	34.0	31.0	34.0	30.0	34.0
4	35.95314239136545	37.0	35.0	37.0	35.0	37.0
5	35.866692467687464	37.0	35.0	37.0	35.0	37.0
6	35.91047579100308	37.0	35.0	37.0	35.0	37.0
7	35.83913459916889	37.0	35.0	37.0	35.0	37.0
8	35.84910986478247	37.0	35.0	37.0	35.0	37.0
9	37.55137941770411	39.0	37.0	39.0	35.0	39.0
10-11	37.490842439364	39.0	37.0	39.0	34.5	39.0
12-13	37.457863978171474	39.0	37.0	39.0	34.0	39.0
14-15	38.673777550517386	40.0	38.0	41.0	34.0	41.0
16-17	38.680383837029794	40.0	38.0	41.0	34.0	41.0
18-19	38.73506401968373	40.0	38.0	41.0	34.0	41.0
20-21	38.73454641373018	40.0	38.0	41.0	34.0	41.0
22-23	38.696343786350255	40.0	38.0	41.0	34.0	41.0
24-25	38.61951216611821	40.0	38.0	41.0	34.0	41.0
26-27	38.47187414867442	40.0	38.0	41.0	34.0	41.0
28-29	38.414861387979606	40.0	38.0	41.0	34.0	41.0
30-31	38.284255611722024	40.0	37.5	41.0	34.0	41.0
32-33	38.134374743251016	40.0	37.0	41.0	33.5	41.0
34-35	38.03007184647384	40.0	37.0	41.0	33.0	41.0
36-37	38.0173512585565	40.0	37.0	41.0	33.0	41.0
38-39	37.91338316245562	40.0	37.0	41.0	33.0	41.0
40-41	37.797979844934424	40.0	37.0	41.0	33.0	41.0
42-43	37.656925520092365	40.0	36.5	41.0	33.0	41.0
44-45	37.44011878559352	40.0	36.0	41.0	32.0	41.0
46-47	37.17963391378423	40.0	36.0	41.0	31.0	41.0
48-49	36.90235571247573	39.0	35.0	41.0	31.0	41.0
50-51	36.68159007078705	39.0	35.0	41.0	30.0	41.0
52-53	36.493964831334836	39.0	35.0	41.0	30.0	41.0
54-55	36.304651967291804	39.0	35.0	41.0	30.0	41.0
56-57	36.071269950747435	38.0	35.0	40.5	30.0	41.0
58-59	35.79028451030671	37.5	35.0	40.0	29.0	41.0
60-61	35.60643602139611	37.0	35.0	40.0	29.0	41.0
62-63	35.39051109804244	37.0	35.0	40.0	29.0	41.0
64-65	35.14377867480768	36.0	34.0	39.5	29.0	41.0
66-67	34.89858263749854	35.5	34.0	39.0	29.0	41.0
68-69	34.65090916166862	35.0	34.0	39.0	29.0	41.0
70-71	34.44145398841981	35.0	34.0	38.5	29.0	41.0
72-73	34.209583385584004	35.0	34.0	37.5	29.0	40.0
74-75	33.9563879147442	35.0	34.0	37.0	28.0	39.5
76-77	33.71666490095435	35.0	34.0	36.5	28.0	39.0
78-79	33.4945608132943	35.0	33.0	36.0	28.0	39.0
80-81	33.366649874382176	35.0	33.0	36.0	28.0	38.5
82-83	33.2054918337607	35.0	33.0	35.5	28.0	37.0
84-85	33.07743592626385	35.0	33.0	35.0	28.0	37.0
86-87	32.93853602269337	35.0	33.0	35.0	28.0	36.5
88-89	32.835091153997496	35.0	33.0	35.0	28.0	36.0
90-91	32.76544720808451	35.0	33.0	35.0	28.0	36.0
92-93	32.697900820299495	35.0	33.0	35.0	27.0	36.0
94-95	32.65330303515137	35.0	33.0	35.0	27.0	35.5
96-97	32.56684100373178	35.0	33.0	35.0	27.0	35.0
98-99	32.49714505939279	35.0	33.0	35.0	27.0	35.0
100	31.275411771319355	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3933774734839517
1101	2	0.4160572435169314
1101	3	0.37806189766141074
1101	4	0.27392689596933195
1101	5	0.24094433677454674
1101	6	0.25090429784282264
1101	7	0.3019352519993319
1101	8	0.30730009715560413
1101	9	0.4179909322401656
1101	10-11	0.4028711775133047
1101	12-13	0.3880495399987609
1101	14-15	0.5315442215140962
1101	16-17	0.5447734500068435
1101	18-19	0.4794738333246116
1101	20-21	0.49920397245191594
1101	22-23	0.4442630818014024
1101	24-25	0.46738797903214646
1101	26-27	0.5566163832456112
1101	28-29	0.5791612797554322
1101	30-31	0.6036673968219475
1101	32-33	0.6475394528429916
1101	34-35	0.6864087402513732
1101	36-37	0.5634761806130513
1101	38-39	0.5311204469805375
1101	40-41	0.5473365437302107
1101	42-43	0.5912399545721314
1101	44-45	0.5866592889452207
1101	46-47	0.6097749390247316
1101	48-49	0.5962889774627698
1101	50-51	0.6082445883239131
1101	52-53	0.6185663618556347
1101	54-55	0.6464959219257906
1101	56-57	0.6377077321759046
1101	58-59	0.5976600491572341
1101	60-61	0.6278523657187804
1101	62-63	0.5856380308334508
1101	64-65	0.5065081368264686
1101	66-67	0.4991726457971879
1101	68-69	0.5154205404799299
1101	70-71	0.4445714924598505
1101	72-73	0.4777709462538695
1101	74-75	0.48790261443441807
1101	76-77	0.4577614102351504
1101	78-79	0.4697803413144612
1101	80-81	0.5030563813387658
1101	82-83	0.45013863513984376
1101	84-85	0.39578038644987146
1101	86-87	0.4429119984205059
1101	88-89	0.4672138268342465
1101	90-91	0.42869145352201343
1101	92-93	0.4324004855242265
1101	94-95	0.4488000959635343
1101	96-97	0.44030516332763625
1101	98-99	0.5266115421735265
1101	100	0.7471387192909553
1102	1	0.33570087332528686
1102	2	0.35898633812679037
1102	3	0.3094017450976594
1102	4	0.23400811306880342
1102	5	0.21772614427629122
1102	6	0.16009019328229357
1102	7	0.2517338686883832
1102	8	0.27441311284077585
1102	9	0.3434553616425049
1102	10-11	0.31207944327344705
1102	12-13	0.3452630240946064
1102	14-15	0.4879611984237968
1102	16-17	0.4475430286444322
1102	18-19	0.455751300113171
1102	20-21	0.45910182626718665
1102	22-23	0.4449278653882658
1102	24-25	0.45974069866262113
1102	26-27	0.49843418175118614
1102	28-29	0.5118085761692441
1102	30-31	0.5180914999623312
1102	32-33	0.5268896436187305
1102	34-35	0.5246195216640857
1102	36-37	0.5074186324130281
1102	38-39	0.5255398771076187
1102	40-41	0.47034528034892986
1102	42-43	0.47718391196803367
1102	44-45	0.5053038647979733
1102	46-47	0.5314574777700898
1102	48-49	0.4972563242207997
1102	50-51	0.5576127826237212
1102	52-53	0.5727326002926318
1102	54-55	0.5453969936750269
1102	56-57	0.5052742877763805
1102	58-59	0.5007348359813761
1102	60-61	0.45372217925390856
1102	62-63	0.5428593995505224
1102	64-65	0.48226526445528606
1102	66-67	0.5252142890659286
1102	68-69	0.5377486188354652
1102	70-71	0.49655074325396953
1102	72-73	0.5011386944308001
1102	74-75	0.49240571872898187
1102	76-77	0.46213884191880084
1102	78-79	0.5379653308476264
1102	80-81	0.47030609435230275
1102	82-83	0.39442601619780504
1102	84-85	0.45623202947199815
1102	86-87	0.4957007522735424
1102	88-89	0.41537221310532857
1102	90-91	0.41381594664859733
1102	92-93	0.3813868945459191
1102	94-95	0.4015848754173845
1102	96-97	0.4196237101846023
1102	98-99	0.40978006705898906
1102	100	0.6806502756244157
1103	1	0.28539871051732035
1103	2	0.3835470157656573
1103	3	0.29722497022954286
1103	4	0.22397817598996284
1103	5	0.22118442381260195
1103	6	0.25089882248399675
1103	7	0.2527267567304392
1103	8	0.21172547489184979
1103	9	0.2893532899980116
1103	10-11	0.3028548784651477
1103	12-13	0.27821033770860737
1103	14-15	0.36799827576324873
1103	16-17	0.3914463416454552
1103	18-19	0.3636583206781481
1103	20-21	0.39587140187256864
1103	22-23	0.37684680638927404
1103	24-25	0.38736751520705326
1103	26-27	0.370219290249409
1103	28-29	0.422628348801112
1103	30-31	0.37650122003198305
1103	32-33	0.4238434270686753
1103	34-35	0.4564865744482134
1103	36-37	0.3935730585459254
1103	38-39	0.4077884931456097
1103	40-41	0.42222444123359537
1103	42-43	0.42171001106117956
1103	44-45	0.43573910452111164
1103	46-47	0.35607815195653814
1103	48-49	0.38722810400023633
1103	50-51	0.35023740167454065
1103	52-53	0.3469185013674334
1103	54-55	0.31297330908806487
1103	56-57	0.3096466583590427
1103	58-59	0.34609692181800256
1103	60-61	0.4108481482907962
1103	62-63	0.3792179448343944
1103	64-65	0.3629905937329809
1103	66-67	0.40648901299384477
1103	68-69	0.3516266047771168
1103	70-71	0.35743078441097254
1103	72-73	0.31472485696101415
1103	74-75	0.25947377609618627
1103	76-77	0.23722976953818886
1103	78-79	0.26555068356468325
1103	80-81	0.26751406632025976
1103	82-83	0.3173802159433876
1103	84-85	0.2760817712250656
1103	86-87	0.3143374104106016
1103	88-89	0.32530963107115696
1103	90-91	0.2770024849157906
1103	92-93	0.3059501177246631
1103	94-95	0.27639760400070656
1103	96-97	0.3041002685670975
1103	98-99	0.30603474071696013
1103	100	0.5372454379767397
1104	1	0.23828342439333738
1104	2	0.2442984197850535
1104	3	0.21206957185110298
1104	4	0.18101950033234715
1104	5	0.16295298780475065
1104	6	0.17028596486559877
1104	7	0.17237024981852755
1104	8	0.19189351800682175
1104	9	0.16281133386737423
1104	10-11	0.26395830052864255
1104	12-13	0.2873507304431939
1104	14-15	0.3540776171650535
1104	16-17	0.2790180677696057
1104	18-19	0.2985494929381005
1104	20-21	0.3362283401963566
1104	22-23	0.29580046955583583
1104	24-25	0.326390080232251
1104	26-27	0.3100136768554478
1104	28-29	0.33276531465880055
1104	30-31	0.3195190451435437
1104	32-33	0.35431327126190126
1104	34-35	0.4022814476993304
1104	36-37	0.3401408845663241
1104	38-39	0.3656820119584836
1104	40-41	0.4069432164732376
1104	42-43	0.4269312648907402
1104	44-45	0.3858958209291288
1104	46-47	0.43782208100465425
1104	48-49	0.3894792470271611
1104	50-51	0.3786870252111214
1104	52-53	0.3641255159528143
1104	54-55	0.3593514309048942
1104	56-57	0.3587048971672786
1104	58-59	0.32939808389178893
1104	60-61	0.3448153931299771
1104	62-63	0.34754687212371493
1104	64-65	0.24811903081982933
1104	66-67	0.27956424708939664
1104	68-69	0.3018876050581909
1104	70-71	0.3162237540669608
1104	72-73	0.2996045730124379
1104	74-75	0.3386131632432523
1104	76-77	0.2602415946701768
1104	78-79	0.31093749423502004
1104	80-81	0.2778314021264947
1104	82-83	0.27344341333569133
1104	84-85	0.31140519789600773
1104	86-87	0.22781557539201458
1104	88-89	0.2567567440715024
1104	90-91	0.2480152153106019
1104	92-93	0.3116930444618049
1104	94-95	0.29269537711191873
1104	96-97	0.2949781308736874
1104	98-99	0.2744369612164803
1104	100	0.6233382795275872
1105	1	0.19918220543955556
1105	2	0.16411661932410482
1105	3	0.14028651714610163
1105	4	0.1504213814737696
1105	5	0.17400447553180953
1105	6	0.15846174205548635
1105	7	0.19725488435536676
1105	8	0.17203146493273636
1105	9	0.23590735487423586
1105	10-11	0.1995619566823308
1105	12-13	0.21146751811333786
1105	14-15	0.31048654059558345
1105	16-17	0.2767188743309177
1105	18-19	0.2766410726137991
1105	20-21	0.29815102565024176
1105	22-23	0.2524416628303001
1105	24-25	0.2583057447022341
1105	26-27	0.29716198316804565
1105	28-29	0.2916187955874392
1105	30-31	0.3305861279844535
1105	32-33	0.3201218734218543
1105	34-35	0.21363374888475306
1105	36-37	0.2598739755969035
1105	38-39	0.2728823415489501
1105	40-41	0.2724772585099515
1105	42-43	0.21649836212857565
1105	44-45	0.2321278111468601
1105	46-47	0.22654008701077544
1105	48-49	0.1951103131379739
1105	50-51	0.2106042548089846
1105	52-53	0.2530105076233795
1105	54-55	0.19571430700034398
1105	56-57	0.12546971008226393
1105	58-59	0.10628965793602418
1105	60-61	0.10458967285944709
1105	62-63	0.16320904121946
1105	64-65	0.13179467409393908
1105	66-67	0.14163529393491103
1105	68-69	0.1451646488296916
1105	70-71	0.16065933881427696
1105	72-73	0.10071877556311648
1105	74-75	0.13330799195216514
1105	76-77	0.1302486354990151
1105	78-79	0.11923156176759164
1105	80-81	0.0683881712802048
1105	82-83	0.07546512794975513
1105	84-85	0.06971720636207834
1105	86-87	0.08175662541233919
1105	88-89	0.07545268701667851
1105	90-91	0.03845386973048193
1105	92-93	0.025330181471481694
1105	94-95	0.07038856434618168
1105	96-97	0.057617905123834134
1105	98-99	0.06306014219060785
1105	100	0.28117882317662435
1106	1	0.22297965373334705
1106	2	0.2307983976836212
1106	3	0.22496630231207604
1106	4	0.1670790004986884
1106	5	0.1603986822512411
1106	6	0.16178555889712243
1106	7	0.21364037666525348
1106	8	0.1542737340425404
1106	9	0.17596622460485634
1106	10-11	0.2248707873744067
1106	12-13	0.1649119960101615
1106	14-15	0.21810914001599713
1106	16-17	0.24723657186689962
1106	18-19	0.3086040369544705
1106	20-21	0.22459317518393362
1106	22-23	0.25625582504385847
1106	24-25	0.22467863424777335
1106	26-27	0.27074592067848613
1106	28-29	0.25175289055008676
1106	30-31	0.29224666860332604
1106	32-33	0.28904228439790813
1106	34-35	0.2597040597167748
1106	36-37	0.1703963345089079
1106	38-39	0.2208877918328369
1106	40-41	0.21079115394401526
1106	42-43	0.20177087438551666
1106	44-45	0.20367735638176754
1106	46-47	0.2044774319948317
1106	48-49	0.25000881680914944
1106	50-51	0.22187034149662566
1106	52-53	0.24275183806456369
1106	54-55	0.2645029653261304
1106	56-57	0.23000470973159537
1106	58-59	0.2205834304074017
1106	60-61	0.19590319088865726
1106	62-63	0.144393753378516
1106	64-65	0.2422140313200245
1106	66-67	0.2054438454481513
1106	68-69	0.22260083776924944
1106	70-71	0.2594966781023871
1106	72-73	0.1971484831363668
1106	74-75	0.18565515752169404
1106	76-77	0.2465763546447164
1106	78-79	0.2786221905031283
1106	80-81	0.18199308304535577
1106	82-83	0.2039004710219885
1106	84-85	0.18795768802985435
1106	86-87	0.19080135137341614
1106	88-89	0.1913582862088603
1106	90-91	0.21530522646988715
1106	92-93	0.24538503497912245
1106	94-95	0.236452787878342
1106	96-97	0.10589286663745412
1106	98-99	0.1397620965974653
1106	100	0.2581494751066771
1107	1	0.10557151457683034
1107	2	0.15487643068124157
1107	3	0.11929533091380051
1107	4	0.09639217545512224
1107	5	0.013677870917867097
1107	6	0.019001933904711166
1107	7	0.08385680114636074
1107	8	0.09181163859405927
1107	9	0.11142648309790815
1107	10-11	0.10635043948312273
1107	12-13	0.03425430804803398
1107	14-15	0.1003822285586935
1107	16-17	0.13298294210009942
1107	18-19	0.13002350232683568
1107	20-21	0.13732996927639363
1107	22-23	0.07136514541057437
1107	24-25	0.04272072815975747
1107	26-27	0.12035434589645888
1107	28-29	0.15316113589364733
1107	30-31	0.07245928603754948
1107	32-33	0.16754571275121322
1107	34-35	0.15812341125984375
1107	36-37	0.12401567366348587
1107	38-39	0.15003687964992451
1107	40-41	0.17548510799961292
1107	42-43	0.12478480459730434
1107	44-45	0.19575107673738756
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2216	60-61	-0.8595929913554343
2216	62-63	-0.8797708619015197
2216	64-65	-0.7739382802229144
2216	66-67	-0.8215584428376701
2216	68-69	-0.7993599466942314
2216	70-71	-0.8672925918534702
2216	72-73	-0.8227614366132414
2216	74-75	-0.7555212131011757
2216	76-77	-0.8522926394642525
2216	78-79	-0.7969069946883849
2216	80-81	-0.9061890777960784
2216	82-83	-0.8233739330957022
2216	84-85	-0.7776851629482096
2216	86-87	-0.8014036785782253
2216	88-89	-0.7431101646995373
2216	90-91	-0.7752804364570558
2216	92-93	-0.787891097573425
2216	94-95	-0.7791146016045545
2216	96-97	-0.8457915744701197
2216	98-99	-0.8411360632266209
2216	100	-0.26321097802890847
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	11.0
13	83.0
14	246.0
15	826.0
16	1936.0
17	4019.0
18	7293.0
19	11172.0
20	15951.0
21	22868.0
22	31382.0
23	37328.0
24	37922.0
25	35701.0
26	36750.0
27	43462.0
28	54562.0
29	69086.0
30	86512.0
31	108542.0
32	137303.0
33	176946.0
34	251527.0
35	369773.0
36	594437.0
37	1118082.0
38	981913.0
39	388706.0
40	796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.506501424821735	22.121514159571387	13.332634255395512	25.039350160211367
2	14.2029434252087	20.964963795316397	39.817367869337936	25.014724910136966
3	17.23614852739593	27.247975196426488	29.77860129639319	25.737274979784395
4	11.570238633926646	17.47310274742908	39.47188180248868	31.484776816155595
5	12.226520658222878	39.042253106960565	35.47584854110477	13.255377693711786
6	29.28491183826811	39.58002532249863	17.57855336578211	13.55650947345116
7	25.659504360949075	32.77805644802103	24.113540346886797	17.448898844143095
8	26.459934185775996	34.62150334908781	19.620033123321672	19.29852934181452
9	26.16405125034053	14.25589279459649	21.725028863991145	37.85502709107183
10-11	25.454970011718565	26.109577223608365	27.562798099084567	20.8726546655885
12-13	25.90192658493486	23.925126812734007	28.10576877132239	22.06717783100875
14-15	23.077235779389607	24.818420497260792	26.106364612938883	25.997979110410718
16-17	21.344564705068386	27.17954483539958	27.41945108688602	24.056439372646018
18-19	20.6669702972138	27.346921611104186	30.274271786671903	21.71183630501011
20-21	22.3108381584125	26.524180889659554	29.493453171147248	21.67152778078069
22-23	22.737149578174932	26.142441093675	29.19741888894174	21.922990439208327
24-25	22.204873798414752	26.191661225389932	30.03680969657135	21.566655279623966
26-27	22.204506241973217	26.38770934501442	28.948637230440593	22.45914718257177
28-29	21.286350251019428	27.073450749598933	29.432233402664572	22.207965596717074
30-31	22.24743467224776	26.561887856367587	29.34870079608401	21.84197667530064
32-33	22.801007104649806	26.79081065654229	28.479732505394434	21.928449733413473
34-35	22.547739095465218	26.700564739661935	29.330214869171527	21.42148129570132
36-37	22.266828679780502	26.109382634904026	29.06079599752656	22.56299268778891
38-39	22.411364845172255	26.208795841855597	29.77788780447727	21.601951508494878
40-41	21.959454200305288	25.50476309906295	30.18890238998171	22.346880310650054
42-43	22.194549786601055	25.681849630497673	29.17565738550617	22.94794319739511
44-45	22.35807997163329	26.727093666353884	28.892909187613782	22.021917174399043
46-47	22.45748236810129	26.52694837345464	28.14292107914571	22.87264817929836
48-49	23.423063950496633	26.318576302555165	28.008557578797618	22.249802168150588
50-51	22.631455480266542	26.32712739506264	27.817039051790864	23.224378072879954
52-53	21.98175622791959	27.026079210575247	27.561803534595708	23.430361026909456
54-55	22.10700649061434	26.958492067267155	27.401127317227154	23.533374124891353
56-57	23.377832887220713	26.244643405388814	27.12630320379491	23.251220503595565
58-59	22.136072249512097	26.744593702437236	28.10428271499329	23.015051333057375
60-61	22.280936360845295	27.477827698188594	27.179761045071064	23.061474895895042
62-63	22.77792672221814	26.611064746148227	26.76613032254159	23.844878209092048
64-65	21.79381319814163	26.84647543337104	27.324947850182134	24.03476351830519
66-67	22.512724221809286	26.397907693731355	27.28356823275772	23.805799851701643
68-69	22.078747886550463	26.261529380732256	27.694761672079117	23.964961060638164
70-71	22.06848433639286	25.960732660543	27.72414789164207	24.246635111422073
72-73	22.658636171454596	25.892660719360872	28.22159513932559	23.22710796985894
74-75	22.792118731660604	26.445333238488917	27.57518537092617	23.187362658924307
76-77	22.45212897523446	26.458367926910693	27.062858259735496	24.026644838119353
78-79	22.190911451526105	26.51181942856204	27.28629344977524	24.010975670136617
80-81	21.849035921074822	26.962167631682497	27.86403222388944	23.324764223353238
82-83	22.70631807902031	25.792548982301078	29.002332902355388	22.498800036323225
84-85	23.039962033581688	26.49310074938272	27.337950851217474	23.128986365818115
86-87	21.527748349239157	27.128822046467782	27.841730196275137	23.50169940801792
88-89	22.546420216469123	25.9147939305621	27.639401185693842	23.899384667274937
90-91	22.13662721560861	25.870968230150872	27.909587601672598	24.082816952567924
92-93	22.54765261159662	26.230741123511937	27.57548960680109	23.64611665809035
94-95	21.334770406949843	26.21982253510164	28.229729262249357	24.215677795699158
96-97	21.666198212378436	26.10539356646501	27.786326467955565	24.442081753200984
98-99	20.97162464271352	26.872883847840285	27.72286892937295	24.43262258007325
100	21.89881504709112	26.046442296313298	26.698554738654177	25.356187917941398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	250.0
2	182.5
3	239.5
4	171.5
5	123.5
6	257.5
7	612.5
8	983.5
9	1052.0
10	843.5
11	651.5
12	646.0
13	773.0
14	1011.0
15	1438.5
16	1927.5
17	2578.0
18	3509.5
19	4648.5
20	6003.5
21	7444.0
22	9021.0
23	10733.0
24	12513.5
25	14445.5
26	17233.5
27	20173.5
28	24424.0
29	34053.0
30	46900.0
31	57065.5
32	72037.0
33	86701.0
34	95176.0
35	108317.0
36	120101.5
37	139904.5
38	148767.0
39	137028.0
40	126408.0
41	117268.5
42	115851.0
43	121779.5
44	132711.5
45	161682.0
46	189722.5
47	210318.0
48	226305.5
49	219731.5
50	204779.5
51	206963.5
52	208890.5
53	197401.0
54	188616.5
55	161974.0
56	137754.0
57	113728.0
58	87735.0
59	86715.0
60	71828.0
61	45863.0
62	32676.0
63	27449.5
64	19894.5
65	8384.5
66	4028.5
67	2748.5
68	1950.5
69	1332.5
70	906.5
71	626.0
72	399.5
73	255.0
74	159.0
75	103.0
76	48.0
77	10.0
78	5.0
79	4.0
80	1.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007005193356309215
3	0.0
4	0.08927297335864427
5	0.004367435364118708
6	8.648386859641005E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.810725729383327E-4
14-15	0.009599709414201515
16-17	0.0
18-19	3.2431450723653775E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.2431450723653775E-5
60-61	0.0
62-63	0.0
64-65	8.648386859641005E-5
66-67	9.72943521709613E-5
68-69	0.0
70-71	5.189032115784604E-4
72-73	4.4322982655660155E-4
74-75	4.648507937057041E-4
76-77	8.648386859641005E-5
78-79	2.1620967149102513E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06337105471401946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4625140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.04220127665195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63057857325103	47.890949116777286
2	15.831099133129475	20.593790724952747
3	5.4648021633408534	10.663282867352962
4	2.2763192159420713	5.92227250452859
5	1.1417684276007563	3.713156593966739
6	0.6053661965668683	2.3624610001910096
7	0.345821922139754	1.5745113345984778
8	0.21151683053641565	1.100601621211922
9	0.140749588035288	0.8239196731137339
>10	0.343522483139985	3.1120229482630495
>50	0.0034625448157807	0.16129200217094677
>100	0.0038707067627057738	0.5313736337186272
>500	6.078663194315665E-4	0.27721493964405736
>1k	3.74071577893244E-4	0.36218899549764505
>5k	4.675894723665548E-5	0.20851186104054528
>10k+	9.351789447331096E-5	0.702450182971627
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12560	0.2715593473927276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10537	0.2278201308500932	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6856	0.14823335077424682	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	4.324193429820503E-5	0.0	0.0
18-19	0.0	0.0	4.324193429820503E-5	0.0	0.0
20-21	0.0	0.0	4.324193429820503E-5	1.0810483574551257E-5	0.0
22-23	0.0	0.0	4.324193429820503E-5	1.0810483574551257E-4	0.0
24-25	0.0	0.0	4.324193429820503E-5	2.1620967149102514E-4	0.0
26-27	0.0	0.0	4.324193429820503E-5	5.405241787275628E-4	0.0
28-29	0.0	0.0	4.324193429820503E-5	0.002367495902826725	0.0
30-31	0.0	0.0	4.324193429820503E-5	0.0098915924707144	0.0
32-33	0.0	0.0	4.324193429820503E-5	0.02349118080749988	0.0
34-35	0.0	0.0	4.324193429820503E-5	0.04585807132324643	0.0
36-37	0.0	0.0	4.324193429820503E-5	0.08371638480132493	0.0
38-39	0.0	0.0	4.324193429820503E-5	0.1403416977648244	0.0
40-41	0.0	0.0	4.324193429820503E-5	0.20383166779816397	0.0
42-43	0.0	0.0	4.324193429820503E-5	0.2721431135057533	0.0
44-45	0.0	0.0	4.324193429820503E-5	0.3524433854975201	0.0
46-47	0.0	0.0	4.324193429820503E-5	0.45337006014953063	0.0
48-49	0.0	0.0	4.324193429820503E-5	0.57070704886771	0.0
50-51	0.0	0.0	4.324193429820503E-5	0.6984004808503095	0.0
52-53	0.0	0.0	4.324193429820503E-5	0.8238561427329767	0.0
54-55	0.0	0.0	4.324193429820503E-5	0.963722179220521	0.0
56-57	0.0	0.0	4.324193429820503E-5	1.1379547429915635	0.0
58-59	0.0	0.0	4.324193429820503E-5	1.3480564912629671	0.0
60-61	0.0	0.0	4.324193429820503E-5	1.5687525134374312	0.0
62-63	0.0	0.0	4.324193429820503E-5	1.8021616642955673	0.0
64-65	0.0	0.0	4.324193429820503E-5	2.0467164237190656	0.0
66-67	0.0	0.0	4.324193429820503E-5	2.323908033054135	0.0
68-69	0.0	0.0	4.324193429820503E-5	2.6354013067712545	0.0
70-71	0.0	0.0	4.324193429820503E-5	2.9866663495591483	0.0
72-73	0.0	0.0	4.324193429820503E-5	3.3572821579454892	0.0
74-75	0.0	0.0	4.324193429820503E-5	3.7112390111434466	0.0
76-77	0.0	0.0	4.324193429820503E-5	4.122307649065758	0.0
78-79	0.0	0.0	4.324193429820503E-5	4.568758999727576	0.0
80-81	0.0	0.0	4.324193429820503E-5	5.057911760508871	0.0
82-83	0.0	0.0	4.324193429820503E-5	5.571344867398609	0.0
84-85	0.0	0.0	4.324193429820503E-5	6.071491889975222	0.0
86-87	0.0	0.0	4.324193429820503E-5	6.603443355228166	0.0
88	0.0	0.0	4.324193429820503E-5	7.031700662034014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5110	0.0	41.68749	94
GTATCAA	19175	0.0	36.361233	1
GGTATCA	11740	0.0	34.302948	1
GTACTAG	5465	0.0	33.83204	1
CTGTGCG	6840	0.0	33.19186	9
TAGTACT	7290	0.0	31.68553	4
CAACGCA	21470	0.0	31.41329	5
ACTGTGC	7760	0.0	31.069227	8
ATCAACG	22020	0.0	30.59375	3
GTACTGT	7815	0.0	29.888046	6
TACCCCG	415	0.0	29.445494	5
TACTGTG	8695	0.0	29.13359	7
TATCAAC	24125	0.0	28.92009	2
CTAACGC	2295	0.0	28.903032	3
TCTAACG	2375	0.0	28.723024	2
AACGCAG	24605	0.0	28.345276	6
CGCCTAC	2400	0.0	28.002682	7
TATTCTC	3040	0.0	27.828676	5
TAACGCC	2430	0.0	27.684505	4
CTAGTAC	8640	0.0	27.551405	3
>>END_MODULE