FastQCFastQC Report
Mon 16 Jan 2017
ERR1146676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000159
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC46350.039.43930494
GTACTAG45300.035.805021
CTGTGCG59100.032.6795739
TAGGCAT52700.032.6373065
CAACGCA63850.031.574775
TAGTACT62700.031.5558664
GCATCGT46550.031.3951688
TACCCCG2550.031.3294335
CGTTAAC8050.030.3689961
GGTATCA51450.030.3372141
CTAACGC21150.030.2203483
ACTGTGC66150.029.9781428
GAACCGT4400.029.9038726
CTAGGCA59600.029.8853594
GTATCAA76600.029.8284341
TCTAACG21700.029.6717132
CGCCTAC22100.029.1306637
TAACGCC21950.029.1185594
GTACTGT68400.028.9233156
TACTGTG73050.028.5617527
Produced by FastQC (version 0.11.5)