##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146676_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000159 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.837698701476615 31.0 31.0 34.0 27.0 34.0 2 30.83756245689234 31.0 31.0 34.0 27.0 34.0 3 31.029467078683624 31.0 31.0 34.0 27.0 34.0 4 34.47200398784148 37.0 35.0 37.0 30.0 37.0 5 34.60836881733951 37.0 35.0 37.0 30.0 37.0 6 34.69424365381476 37.0 35.0 37.0 31.0 37.0 7 34.55384748456249 37.0 35.0 37.0 30.0 37.0 8 34.56652498063202 37.0 35.0 37.0 30.0 37.0 9 35.94996973870288 39.0 35.0 39.0 30.0 39.0 10-11 35.85405280140114 39.0 35.0 39.0 30.0 39.0 12-13 35.79727905815744 39.0 35.0 39.0 30.0 39.0 14-15 36.880137264543734 40.0 36.0 41.0 29.5 41.0 16-17 36.87810472033736 40.0 36.0 41.0 29.5 41.0 18-19 36.82371800720921 40.0 36.0 41.0 29.0 41.0 20-21 36.91690030321295 40.0 36.0 41.0 29.5 41.0 22-23 36.83663561873415 40.0 36.0 41.0 29.0 41.0 24-25 36.7850617937937 40.0 36.0 41.0 29.0 41.0 26-27 36.862030734278314 40.0 36.0 41.0 29.0 41.0 28-29 36.85470452549511 40.0 36.0 41.0 29.0 41.0 30-31 36.72286251621498 40.0 36.0 41.0 29.0 41.0 32-33 36.72680573447205 40.0 35.5 41.0 29.0 41.0 34-35 36.65462635360244 39.5 35.0 41.0 29.0 41.0 36-37 36.51641609746012 39.0 35.0 41.0 29.0 41.0 38-39 36.39504179708857 39.0 35.0 41.0 28.0 41.0 40-41 36.314423876650906 39.0 35.0 41.0 28.0 41.0 42-43 36.11462669358893 39.0 35.0 40.5 27.5 41.0 44-45 35.92200185042645 38.5 34.5 40.0 27.0 41.0 46-47 35.937223245375996 38.5 34.5 40.0 27.0 41.0 48-49 35.80253172436396 38.0 34.0 40.0 27.0 41.0 50-51 35.623983446657995 38.0 34.0 40.0 27.0 41.0 52-53 35.40787653690766 38.0 34.0 40.0 27.0 41.0 54-55 35.19188887241732 38.0 33.0 40.0 26.0 41.0 56-57 34.949429010196845 37.0 33.0 40.0 26.0 41.0 58-59 34.65176659227796 37.0 33.0 40.0 26.0 41.0 60-61 34.40732955865004 36.0 33.0 39.5 26.0 41.0 62-63 34.105800294438296 35.5 32.5 39.0 26.0 41.0 64-65 33.96453928456344 35.0 32.0 39.0 26.0 41.0 66-67 33.715814671366815 35.0 32.0 39.0 25.0 40.0 68-69 33.60137122049399 35.0 32.0 38.0 25.0 40.0 70-71 33.5429039195692 35.0 32.0 38.0 25.5 40.0 72-73 33.335298046902636 35.0 32.0 37.0 25.0 40.0 74-75 33.09823059533383 35.0 32.0 37.0 25.0 39.5 76-77 32.83528754731999 35.0 32.0 36.0 25.0 39.0 78-79 32.585036244809274 35.0 32.0 36.0 25.0 39.0 80-81 32.40115842895245 35.0 31.5 35.5 24.5 38.0 82-83 32.14748813734654 35.0 31.0 35.0 24.0 37.0 84-85 31.96840100606001 35.0 31.0 35.0 24.0 37.0 86-87 31.793634703020555 35.0 31.0 35.0 24.0 36.0 88-89 31.664432463809565 35.0 31.0 35.0 24.0 36.0 90-91 31.516859579831703 34.0 31.0 35.0 23.0 36.0 92-93 31.35858749614703 34.0 31.0 35.0 23.0 35.5 94-95 31.278262439068044 34.0 31.0 35.0 23.0 35.0 96-97 31.17470743037964 34.0 31.0 35.0 23.0 35.0 98-99 31.066862342221896 34.0 30.0 35.0 21.5 35.0 100 29.986943019014994 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3817303235817455 1101 2 0.4028509111770191 1101 3 0.46607438985323313 1101 4 0.6708174076453872 1101 5 0.5006358043620693 1101 6 0.470122676058061 1101 7 0.48084419473300244 1101 8 0.42535102979643113 1101 9 0.6135132718132326 1101 10-11 0.617272152912534 1101 12-13 0.5818967232309049 1101 14-15 0.7640674828975236 1101 16-17 0.7061011999167093 1101 18-19 0.6754086987292354 1101 20-21 0.6968980891237848 1101 22-23 0.6992180093236158 1101 24-25 0.7227955607640055 1101 26-27 0.6842246577948572 1101 28-29 0.744176034044898 1101 30-31 0.7772576736619357 1101 32-33 0.7045386618357057 1101 34-35 0.691520565058596 1101 36-37 0.7781936988566045 1101 38-39 0.6488693117944067 1101 40-41 0.6528712722135452 1101 42-43 0.669190867536436 1101 44-45 0.7117559856578595 1101 46-47 0.6222514233319174 1101 48-49 0.6015468882635275 1101 50-51 0.5983785646565067 1101 52-53 0.5117251019083113 1101 54-55 0.46946238487890213 1101 56-57 0.46365434861902344 1101 58-59 0.3600756825451583 1101 60-61 0.437428907887913 1101 62-63 0.41373958009631195 1101 64-65 0.532976648766649 1101 66-67 0.6185428704617237 1101 68-69 0.5370233473509529 1101 70-71 0.5024314916504267 1101 72-73 0.4582332873971353 1101 74-75 0.489442275843615 1101 76-77 0.5106691847047671 1101 78-79 0.46937649246275726 1101 80-81 0.453640628768909 1101 82-83 0.4936630218021776 1101 84-85 0.48040945375585054 1101 86-87 0.5035098579349437 1101 88-89 0.4916564788889417 1101 90-91 0.5459385831380494 1101 92-93 0.41962037046203804 1101 94-95 0.3785935481649929 1101 96-97 0.43596110029209 1101 98-99 0.43483184210012027 1101 100 0.47675392788415394 1102 1 0.4278667294252294 1102 2 0.5419917124217157 1102 3 0.5971101799115317 1102 4 0.7686288701961104 1102 5 0.5747961775753865 1102 6 0.5824984298764235 1102 7 0.5538178341002578 1102 8 0.5508990909666878 1102 9 0.7516053927916317 1102 10-11 0.872633433721461 1102 12-13 0.8469530329335129 1102 14-15 1.063237167907559 1102 16-17 1.0048312748575796 1102 18-19 1.057328809284023 1102 20-21 0.9862148498099756 1102 22-23 1.021429830053549 1102 24-25 1.0240860504671687 1102 26-27 0.9762497378005293 1102 28-29 0.9649324981805236 1102 30-31 0.970131173471735 1102 32-33 0.966768102408615 1102 34-35 0.9936594395920011 1102 36-37 1.0120517531498336 1102 38-39 1.0052291902671513 1102 40-41 0.8686476184195584 1102 42-43 0.934842630937176 1102 44-45 0.8994331823004984 1102 46-47 0.8346722235134081 1102 48-49 0.8568146139316397 1102 50-51 0.8704175852525111 1102 52-53 0.7893836096729174 1102 54-55 0.7739167986938682 1102 56-57 0.7881902072844227 1102 58-59 0.7835908283938409 1102 60-61 0.7437988763263093 1102 62-63 0.7616824103858875 1102 64-65 0.7497813204382382 1102 66-67 0.9097608115048388 1102 68-69 0.8207734081180789 1102 70-71 0.7559973881027844 1102 72-73 0.8121055084451427 1102 74-75 0.6982869613695186 1102 76-77 0.7026133584578247 1102 78-79 0.7157235226961589 1102 80-81 0.7305788144100589 1102 82-83 0.7444915589530154 1102 84-85 0.7972072938620123 1102 86-87 0.7355274698505028 1102 88-89 0.768376614018706 1102 90-91 0.725502456467769 1102 92-93 0.7090700085930841 1102 94-95 0.7647472590864872 1102 96-97 0.7201642827057277 1102 98-99 0.6704260699636038 1102 100 0.6532722287269408 1103 1 0.41852398129501367 1103 2 0.4933265366781079 1103 3 0.502615902182022 1103 4 0.6410842695145291 1103 5 0.4594051418945071 1103 6 0.538986686372958 1103 7 0.49384045364142537 1103 8 0.5600830623788369 1103 9 0.6560543901255897 1103 10-11 0.8036848895487623 1103 12-13 0.68471919189804 1103 14-15 0.8610387244776874 1103 16-17 0.842659839101799 1103 18-19 0.9106014464391379 1103 20-21 0.7830703226882392 1103 22-23 0.8905221330339188 1103 24-25 0.9113674100693032 1103 26-27 0.8360061930734517 1103 28-29 0.812772381127914 1103 30-31 0.8125062788902042 1103 32-33 0.8537582038749676 1103 34-35 0.8411870951577356 1103 36-37 0.9066210757683777 1103 38-39 0.8639438491880824 1103 40-41 0.7594752035406813 1103 42-43 0.7374410420757513 1103 44-45 0.6846202524267753 1103 46-47 0.6999354841328795 1103 48-49 0.7364235296798256 1103 50-51 0.7038507141822237 1103 52-53 0.6621360286451505 1103 54-55 0.6411658067022685 1103 56-57 0.7226634688719713 1103 58-59 0.6453553178580194 1103 60-61 0.735386624525205 1103 62-63 0.6481923183545675 1103 64-65 0.6259108264115127 1103 66-67 0.638497722614332 1103 68-69 0.6594940027424698 1103 70-71 0.6137701504815922 1103 72-73 0.5967086107546322 1103 74-75 0.5672297328584435 1103 76-77 0.5342026117974683 1103 78-79 0.5234615652293471 1103 80-81 0.5201107502477385 1103 82-83 0.5270458274841872 1103 84-85 0.5466021502332801 1103 86-87 0.48001778932779615 1103 88-89 0.5556513746728484 1103 90-91 0.5424246490648237 1103 92-93 0.46831291832494415 1103 94-95 0.4219731127151185 1103 96-97 0.4676492740070657 1103 98-99 0.518189847277359 1103 100 0.5284448537449045 1104 1 0.3660650707579123 1104 2 0.4801481335155664 1104 3 0.407405741171214 1104 4 0.6363299885481482 1104 5 0.48028265442517437 1104 6 0.5309019580920875 1104 7 0.513163150888829 1104 8 0.5639537089738411 1104 9 0.5586565700610251 1104 10-11 0.7826205320775301 1104 12-13 0.6295928785808087 1104 14-15 0.7404655081445242 1104 16-17 0.7425905426657096 1104 18-19 0.7515100353937498 1104 20-21 0.7023935472334131 1104 22-23 0.7724738842503314 1104 24-25 0.7868547823249301 1104 26-27 0.6874183267657372 1104 28-29 0.6824029878592057 1104 30-31 0.6479061944879803 1104 32-33 0.7317631312565638 1104 34-35 0.7683694444957965 1104 36-37 0.7249747725925602 1104 38-39 0.6846227995301106 1104 40-41 0.7036087608055723 1104 42-43 0.7174384008325561 1104 44-45 0.641055623526853 1104 46-47 0.6919774369810483 1104 48-49 0.6589636380230957 1104 50-51 0.5388219734280852 1104 52-53 0.4661852930388193 1104 54-55 0.5875437615579742 1104 56-57 0.6750366265281329 1104 58-59 0.644776403251214 1104 60-61 0.5858803632531249 1104 62-63 0.5729188755599779 1104 64-65 0.5380482789374241 1104 66-67 0.49034504985429805 1104 68-69 0.5691076006628748 1104 70-71 0.5158370583452125 1104 72-73 0.5153205235696561 1104 74-75 0.4409983520885987 1104 76-77 0.4273941472678402 1104 78-79 0.4241037534079126 1104 80-81 0.4526866210497573 1104 82-83 0.43796453061520424 1104 84-85 0.5445895691677372 1104 86-87 0.5422734062592767 1104 88-89 0.44552582896287873 1104 90-91 0.45792378697618474 1104 92-93 0.47198141969972696 1104 94-95 0.5703700496855753 1104 96-97 0.5656493696986438 1104 98-99 0.5220715908972267 1104 100 0.7323023870493763 1105 1 0.1708980676928462 1105 2 0.23182113136669003 1105 3 0.25421603537868265 1105 4 0.29796380894193675 1105 5 0.23796258476711074 1105 6 0.2553613029342259 1105 7 0.28396791118067455 1105 8 0.2907849922203667 1105 9 0.37770436593429224 1105 10-11 0.4401482502413714 1105 12-13 0.2335916497086714 1105 14-15 0.4905834587009039 1105 16-17 0.41735301046031026 1105 18-19 0.4635025833825779 1105 20-21 0.41248157765085836 1105 22-23 0.4456718912633377 1105 24-25 0.4305287543201075 1105 26-27 0.4297875027238476 1105 28-29 0.4512693940831838 1105 30-31 0.4952164012411302 1105 32-33 0.5051315700996142 1105 34-35 0.5249403619749984 1105 36-37 0.5093137844419289 1105 38-39 0.4514263507311469 1105 40-41 0.4391120778233457 1105 42-43 0.43257193005896255 1105 44-45 0.4137452501940331 1105 46-47 0.4411577261633681 1105 48-49 0.3994081246522967 1105 50-51 0.47636843354058556 1105 52-53 0.46208096939395205 1105 54-55 0.46943005253657333 1105 56-57 0.40982438840526214 1105 58-59 0.4946936252991492 1105 60-61 0.5480217918265424 1105 62-63 0.43879499442012815 1105 64-65 0.47449032448031403 1105 66-67 0.42788816969726895 1105 68-69 0.38508165449032106 1105 70-71 0.4352969133376021 1105 72-73 0.3932088589667302 1105 74-75 0.38015091790144595 1105 76-77 0.37188051619185103 1105 78-79 0.42074977576290706 1105 80-81 0.29134887000030574 1105 82-83 0.3855608206082053 1105 84-85 0.23914651755302074 1105 86-87 0.3111607217028727 1105 88-89 0.2757279994266355 1105 90-91 0.24989626905498952 1105 92-93 0.2777345090586145 1105 94-95 0.2421142152324549 1105 96-97 0.127752658467287 1105 98-99 0.2415141545723074 1105 100 0.2339760789824794 1106 1 0.13043654230745716 1106 2 0.2611044828693032 1106 3 0.2104079418100504 1106 4 0.35196705956285257 1106 5 0.23531882104231983 1106 6 0.2943773889344641 1106 7 0.26792191630258344 1106 8 0.15406035174608945 1106 9 0.3695293044244181 1106 10-11 0.3972754403378147 1106 12-13 0.30169943314179903 1106 14-15 0.4538317195172752 1106 16-17 0.3765497890629703 1106 18-19 0.35965181874689023 1106 20-21 0.380577973767366 1106 22-23 0.3723185860034164 1106 24-25 0.4683268193550916 1106 26-27 0.4410895401812809 1106 28-29 0.42517953016347576 1106 30-31 0.4938928142324528 1106 32-33 0.3727212448355246 1106 34-35 0.41957573349446164 1106 36-37 0.34278787614329076 1106 38-39 0.3631136441297258 1106 40-41 0.410258729235224 1106 42-43 0.4095097173159914 1106 44-45 0.41277068258196437 1106 46-47 0.3993481842697193 1106 48-49 0.3770439288416938 1106 50-51 0.37962095853431066 1106 52-53 0.32538561152790635 1106 54-55 0.4065174235324136 1106 56-57 0.3463307089453025 1106 58-59 0.2617973464617336 1106 60-61 0.18897553391870758 1106 62-63 0.35938958942121246 1106 64-65 0.2919643946706003 1106 66-67 0.33029848359370817 1106 68-69 0.19008934557447787 1106 70-71 0.24918021872256446 1106 72-73 0.24785353315218828 1106 74-75 0.23199047343015167 1106 76-77 0.21684895524585102 1106 78-79 0.23188445092684873 1106 80-81 0.2588127770815518 1106 82-83 0.28492495625774694 1106 84-85 0.2597374158844197 1106 86-87 0.24665135437247798 1106 88-89 0.2813246283611832 1106 90-91 0.2568827780250622 1106 92-93 0.2968205502152479 1106 94-95 0.2996798662061515 1106 96-97 0.3299320496496172 1106 98-99 0.36355653462772963 1106 100 0.3244402101811552 1107 1 0.16154928147373937 1107 2 0.2067724110711815 1107 3 0.2099796544863608 1107 4 0.26827462420883563 1107 5 0.19160507272347616 1107 6 0.2880509892082088 1107 7 0.19310025427692068 1107 8 0.21114753852567958 1107 9 0.26031514739805317 1107 10-11 0.3917357795195784 1107 12-13 0.3106237646227541 1107 14-15 0.47686894108799294 1107 16-17 0.24392354787196524 1107 18-19 0.25165672399321437 1107 20-21 0.2910454427100291 1107 22-23 0.4600577472820788 1107 24-25 0.4937405241180457 1107 26-27 0.2945440721560715 1107 28-29 0.37048289156741276 1107 30-31 0.5322052389833587 1107 32-33 0.4342262509383943 1107 34-35 0.40093987576314305 1107 36-37 0.42243278503561754 1107 38-39 0.42994804776997597 1107 40-41 0.3817695167722235 1107 42-43 0.45236594590899415 1107 44-45 0.38750118567436687 1107 46-47 0.40356362337841034 1107 48-49 0.4118168123657071 1107 50-51 0.34270273577371313 1107 52-53 0.375529918338529 1107 54-55 0.3060886269782159 1107 56-57 0.38091753431577047 1107 58-59 0.3274354808981883 1107 60-61 0.32623292643921076 1107 62-63 0.3802594347492345 1107 64-65 0.3558313631130545 1107 66-67 0.32453362725486556 1107 68-69 0.3396503588506974 1107 70-71 0.3169787914139235 1107 72-73 0.2995057738075957 1107 74-75 0.3640759073671518 1107 76-77 0.3129199616372276 1107 78-79 0.3284811433167789 1107 80-81 0.27304771571627384 1107 82-83 0.2572838460916387 1107 84-85 0.21149909884990947 1107 86-87 0.3106726708062979 1107 88-89 0.3436753462683555 1107 90-91 0.24725505243822354 1107 92-93 0.2876278609242071 1107 94-95 0.24740374034029244 1107 96-97 0.30392386829435125 1107 98-99 0.27430227914508976 1107 100 0.3491940166848124 1108 1 0.03757990674151657 1108 2 0.06652387842218133 1108 3 0.058703134823197445 1108 4 0.1794909891492651 1108 5 0.0696712946607363 1108 6 0.0709927425983139 1108 7 0.16714963898858315 1108 8 0.21343088301970425 1108 9 0.17284853335607409 1108 10-11 0.25324344202019233 1108 12-13 0.1810733453667197 1108 14-15 0.28740850523231387 1108 16-17 0.28144166685496685 1108 18-19 0.19621512619756487 1108 20-21 0.11233222191410874 1108 22-23 0.2989602257563959 1108 24-25 0.3931416041140565 1108 26-27 0.24631811303740392 1108 28-29 0.2327543540597219 1108 30-31 0.3325812594807829 1108 32-33 0.2020016058324714 1108 34-35 0.280304968816651 1108 36-37 0.3310222275154331 1108 38-39 0.3603870968916354 1108 40-41 0.26810684595842105 1108 42-43 0.3045596339014267 1108 44-45 0.3290262814393259 1108 46-47 0.29059215295094987 1108 48-49 0.3137938079172784 1108 50-51 0.28683814165864874 1108 52-53 0.28800343970483766 1108 54-55 0.2773600406028365 1108 56-57 0.23300048451238098 1108 58-59 0.2774185978131669 1108 60-61 0.23184357903411978 1108 62-63 0.30037526788753155 1108 64-65 0.30033638522083805 1108 66-67 0.2683475826772366 1108 68-69 0.2372742192257391 1108 70-71 0.23377295863060255 1108 72-73 0.25352642485223953 1108 74-75 0.31408509596361256 1108 76-77 0.2980039515591244 1108 78-79 0.15557084373912033 1108 80-81 0.15381486357927088 1108 82-83 0.27471687646534804 1108 84-85 0.24263179508493593 1108 86-87 0.21083825272388879 1108 88-89 0.304048797033893 1108 90-91 0.26710305587219807 1108 92-93 0.1892700693453726 1108 94-95 0.15117727529730018 1108 96-97 0.13079359726050654 1108 98-99 0.19054683526101002 1108 100 0.1811003934103148 1109 1 0.20017614443266396 1109 2 0.2349142498677672 1109 3 0.16138569400140312 1109 4 0.24593241442415348 1109 5 0.28280820473964496 1109 6 0.24509782511814393 1109 7 0.2405328723623228 1109 8 0.3102966447330502 1109 9 0.31361782343029887 1109 10-11 0.44470218077474044 1109 12-13 0.3071766892277168 1109 14-15 0.3537678160960027 1109 16-17 0.33180325605295025 1109 18-19 0.398944474971465 1109 20-21 0.42986715460089187 1109 22-23 0.5825255464246339 1109 24-25 0.5279493746429864 1109 26-27 0.41984616818532317 1109 28-29 0.44803009365435287 1109 30-31 0.40204197615914694 1109 32-33 0.4153449484173919 1109 34-35 0.37347880545716095 1109 36-37 0.3281053880462821 1109 38-39 0.3317631297187873 1109 40-41 0.3644872985414196 1109 42-43 0.45264378605268263 1109 44-45 0.41922133556637675 1109 46-47 0.3993996629469123 1109 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2215 80-81 -1.3501481956422552 2215 82-83 -1.1763746831033899 2215 84-85 -1.2152959628078257 2215 86-87 -1.1321412399229374 2215 88-89 -1.1175509319476333 2215 90-91 -1.2974102947597714 2215 92-93 -1.3074577425166822 2215 94-95 -1.2314447391775758 2215 96-97 -1.430862255316832 2215 98-99 -1.3018362395345946 2215 100 -0.8641755985300108 2216 1 -1.4318293500728316 2216 2 -1.9615715559930358 2216 3 -1.720367969002261 2216 4 -1.8346651130483735 2216 5 -1.4432105681541287 2216 6 -1.424922779277459 2216 7 -1.4324295818501298 2216 8 -1.403416725230322 2216 9 -1.7759197223572443 2216 10-11 -2.734722040836985 2216 12-13 -1.8886451972191765 2216 14-15 -2.027442119801428 2216 16-17 -2.162719408782216 2216 18-19 -2.4590454517684606 2216 20-21 -2.6018636146298775 2216 22-23 -2.460005360168765 2216 24-25 -2.3164817151005934 2216 26-27 -2.7955727878586316 2216 28-29 -2.8014204557961904 2216 30-31 -2.8597540403243684 2216 32-33 -3.2832241907767497 2216 34-35 -2.6806306267772655 2216 36-37 -2.752606608290705 2216 38-39 -2.5725466230284653 2216 40-41 -2.5164192948651802 2216 42-43 -2.523798521639627 2216 44-45 -2.3320836094885635 2216 46-47 -2.3326916832069315 2216 48-49 -2.4188508921794067 2216 50-51 -2.43807124439968 2216 52-53 -2.308092635204318 2216 54-55 -2.2600017992098813 2216 56-57 -2.3943733512692305 2216 58-59 -2.3015082721755604 2216 60-61 -2.276164946979094 2216 62-63 -2.3392025132713172 2216 64-65 -2.290960075129888 2216 66-67 -2.094438042446484 2216 68-69 -2.224179988320465 2216 70-71 -2.3407793500105853 2216 72-73 -2.1277650170689455 2216 74-75 -2.1576681396350885 2216 76-77 -2.1379702740812547 2216 78-79 -1.9955121063612467 2216 80-81 -2.058369613399755 2216 82-83 -2.111340233017394 2216 84-85 -2.0629919952976756 2216 86-87 -2.037080244217613 2216 88-89 -1.988165111582429 2216 90-91 -2.0299022873033117 2216 92-93 -2.081161830524895 2216 94-95 -2.133819726732977 2216 96-97 -2.2135086038136933 2216 98-99 -2.1219931444853124 2216 100 -1.4324674441414444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 5.0 2 16.0 3 14.0 4 33.0 5 25.0 6 43.0 7 164.0 8 493.0 9 779.0 10 1161.0 11 2468.0 12 4945.0 13 8258.0 14 11540.0 15 14576.0 16 16927.0 17 19795.0 18 22392.0 19 25250.0 20 28030.0 21 31545.0 22 36492.0 23 42702.0 24 50188.0 25 55375.0 26 58404.0 27 60437.0 28 66824.0 29 78248.0 30 91122.0 31 106440.0 32 131655.0 33 171104.0 34 245607.0 35 344678.0 36 562709.0 37 850068.0 38 674209.0 39 185299.0 40 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22201940782085 20.67127009225693 11.66069978805367 24.446010711868542 2 16.0085932513526 20.0535865674071 39.71788674818739 24.21993343305292 3 18.73896208055906 26.517032647240597 28.828045465727698 25.915959806472642 4 12.17016089090655 16.467085405212423 40.12022128156196 31.242532422319062 5 12.973638776792843 38.85829198793413 35.154730875325804 13.013338359947221 6 29.794772770567597 38.7747265411917 18.082880881108103 13.347619807132604 7 26.917285752105997 31.901884967979722 23.70358296661526 17.477246313299023 8 26.29637944232422 35.10510481982709 19.86549342991754 18.733022307931147 9 26.14203731276868 15.196873503174396 21.176651170511374 37.48443801354555 10-11 25.5993860207218 26.186941190734814 27.51060901694568 20.70306377159771 12-13 25.46511415705473 24.181261338645804 27.522188559570438 22.83143594472903 14-15 22.987770717770044 25.376891833887356 24.778689715118396 26.856647733224204 16-17 22.10468475751992 27.80965580480498 26.099366947631626 23.986292490043482 18-19 21.488944798490444 28.605765144341106 27.659578191388835 22.245711865779626 20-21 23.140880669648823 27.755959717773926 26.742829258024713 22.360330354552538 22-23 23.62948837432552 27.314522693364584 26.436672273176352 22.619316659133545 24-25 23.04832867096155 27.30073672973993 27.380059548362418 22.270875050936098 26-27 23.012988909077635 27.52421983046119 26.259691182161728 23.203100078299453 28-29 22.163718106746657 27.985425512752677 26.846864008993993 23.003992371506676 30-31 23.015210905911783 27.705108977367015 26.871612934032573 22.408067182688622 32-33 23.389262445484775 27.779593788817238 25.935554372474712 22.895589393223283 34-35 23.27085023431162 27.55935915240318 27.007661221270418 22.16212939201478 36-37 22.946152052807278 27.146758203947545 26.52474387957981 23.38234586366537 38-39 23.096221631485378 27.341851710829847 27.16267227613883 22.399254381545944 40-41 22.664405450818556 26.414837022705246 27.88619402472004 23.034563501756164 42-43 22.7270869115875 26.63820023405048 27.131528945213912 23.50318390914811 44-45 22.808222791707497 27.615261382989072 27.058604668840204 22.51791115646323 46-47 22.779887542906522 27.35394529968555 26.608406977860632 23.25776017954729 48-49 23.535037086972352 27.009952871139774 26.790724156141355 22.664285885746523 50-51 22.99982459783459 26.742154191130354 26.900041143872155 23.357980067162902 52-53 22.500610761352473 27.452216207268272 26.678618501580477 23.368554529798775 54-55 22.54587388511891 27.258076070392733 26.656267506700758 23.539782537787595 56-57 23.60843682718371 26.716752764649097 26.35660891713566 23.318201491031534 58-59 22.48611114931099 27.11953275353419 27.33238803219758 23.061968064957238 60-61 22.514823783562132 27.795353651782666 26.57510904528958 23.11471351936562 62-63 22.976755698566638 27.11606399495961 26.133475344339185 23.773704962134566 64-65 22.08780060942261 27.350136729431984 26.71863739354637 23.84342526759903 66-67 22.8036680768949 26.92444148636272 26.63157389421763 23.640316542524744 68-69 22.2466948788341 26.95161589118207 26.96451612016113 23.8371731098227 70-71 22.245080289569223 26.64142125981939 27.031990446530685 24.0815080040807 72-73 22.80867112394222 26.699613920850336 27.49416068567957 22.99755426952787 74-75 22.96061630606888 26.874921400374678 27.033095723905358 23.13136656965108 76-77 22.67227405287539 26.790207675553653 26.810846597059374 23.726671674511582 78-79 22.547580636592006 26.784018381573922 26.73533921342015 23.93306176841392 80-81 22.090387774952745 27.16094771292895 27.408625352224654 23.34003915989365 82-83 22.879588409864592 26.153744035946886 28.460300834908058 22.506366719280464 84-85 23.04123996152747 26.647527074905174 27.202988596500223 23.108244367067137 86-87 21.794932855807964 27.317188725651047 27.62994284071341 23.25793557782757 88-89 22.912362854060593 26.085835826138265 27.413112258100004 23.58868906170114 90-91 22.579220433084952 26.16516373635468 27.519913712039934 23.735702118520432 92-93 22.89076652987711 26.351936513111347 27.48282546855882 23.274471488452726 94-95 21.712586584237155 26.425774189209527 28.094581763070824 23.76705746348249 96-97 22.065097031513957 26.282888346692808 27.681239207226994 23.97077541456624 98-99 21.459011309177736 26.925465982149877 27.525395303695465 24.09012740497692 100 22.28681631410172 26.24083187031725 26.650451310699502 24.821900504881526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1219.0 1 948.0 2 639.5 3 504.5 4 365.0 5 299.0 6 266.0 7 269.5 8 311.0 9 319.5 10 273.0 11 244.5 12 255.0 13 272.5 14 298.5 15 443.5 16 533.0 17 669.5 18 937.5 19 1167.0 20 1534.0 21 2065.5 22 2991.5 23 4158.5 24 5277.0 25 6742.5 26 9250.5 27 12246.5 28 15974.0 29 23243.0 30 33836.0 31 43830.0 32 57112.5 33 70351.5 34 80013.0 35 92304.5 36 103497.5 37 120447.5 38 127669.5 39 121142.0 40 113936.5 41 105851.0 42 104815.0 43 111459.5 44 122709.5 45 144717.5 46 166113.0 47 181557.0 48 192880.5 49 189674.0 50 180108.5 51 183415.5 52 184375.0 53 174322.5 54 163087.5 55 139701.5 56 121662.0 57 100288.5 58 78776.0 59 76366.0 60 64153.0 61 43945.0 62 32707.0 63 27343.0 64 20349.0 65 10419.5 66 5653.5 67 3925.5 68 2942.0 69 2192.0 70 1737.5 71 1392.5 72 1022.0 73 782.0 74 633.5 75 499.0 76 372.5 77 264.0 78 186.5 79 129.0 80 80.0 81 50.0 82 34.5 83 29.5 84 26.0 85 23.5 86 21.0 87 14.5 88 12.5 89 10.5 90 11.0 91 10.5 92 7.0 93 6.0 94 7.0 95 6.5 96 5.0 97 4.5 98 4.5 99 4.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049423035434341483 2 0.006899725735901998 3 0.007774690956034498 4 0.009199634314535998 5 0.0029248837358714992 6 0.002624895660397499 7 9.499622390009996E-4 8 0.0014249433585014994 9 6.749731698164998E-4 10-11 5.999761509479998E-4 12-13 2.999880754739999E-4 14-15 1.4999403773699994E-4 16-17 3.999841006319999E-4 18-19 0.0012749493207644996 20-21 8.124677044087498E-4 22-23 0.0011749532956064996 24-25 0.0012249513081854996 26-27 0.0032748698239244995 28-29 0.003887345478017249 30-31 0.004999801257899999 32-33 0.006112257037782748 34-35 0.003599856905687999 36-37 0.008287170584969247 38-39 0.0064497436226909986 40-41 0.006149755547216998 42-43 0.014861909239107747 44-45 0.020899169258021995 46-47 0.019174237824046494 48-49 0.022774094729734493 50-51 0.019461726396375743 52-53 0.02629895461655399 54-55 0.019936707515876246 56-57 0.016149358063016993 58-59 0.018199276578755995 60-61 0.016849330239122995 62-63 0.011724533949775497 64-65 0.011787031465499247 66-67 0.014249433585014994 68-69 0.005749771446584998 70-71 0.008887146735917248 72-73 0.008287170584969247 74-75 0.011449544880590997 76-77 0.010862068232787746 78-79 0.012537001654184247 80-81 0.010087099037813246 82-83 0.006437244119546248 84-85 0.010549580654168996 86-87 0.009287130836549248 88-89 0.007849687974902998 90-91 0.013199475320855997 92-93 0.008524661144719498 94-95 0.007424704867981497 96-97 0.009399626364851997 98-99 0.008862147729627748 100 0.25283994961200296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4000159.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.7952517308722 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06623660139086 55.26718871877294 2 13.8373818806423 19.592418670725625 3 4.368730435193566 9.278561127115541 4 1.7480092911016547 4.950030311657803 5 0.8172363857061824 2.8928227824848673 6 0.42244535553357787 1.794427517252247 7 0.2387209571475272 1.183021717828576 8 0.1327251458120934 0.7517048087027096 9 0.09059290427133372 0.577219271662789 >10 0.26753210247243536 2.916020841066622 >50 0.007941689451823289 0.38016910820430516 >100 0.0023519859309815306 0.2720863741350557 >500 0.0 0.0 >1k 9.526534570504273E-5 0.1443287503909252 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.4999006289499993E-5 0.0 3 0.0 0.0 0.0 2.4999006289499993E-5 0.0 4 0.0 0.0 0.0 2.4999006289499993E-5 0.0 5 0.0 0.0 0.0 2.4999006289499993E-5 0.0 6 0.0 0.0 0.0 2.4999006289499993E-5 0.0 7 0.0 0.0 0.0 2.4999006289499993E-5 0.0 8 0.0 0.0 0.0 2.4999006289499993E-5 0.0 9 0.0 0.0 0.0 4.999801257899999E-5 0.0 10-11 0.0 0.0 0.0 9.999602515799997E-5 0.0 12-13 0.0 0.0 0.0 9.999602515799997E-5 0.0 14-15 0.0 0.0 0.0 9.999602515799997E-5 0.0 16-17 0.0 0.0 0.0 1.2499503144749996E-4 0.0 18-19 0.0 0.0 0.0 1.2499503144749996E-4 0.0 20-21 0.0 0.0 0.0 1.3749453459224997E-4 0.0 22-23 0.0 0.0 0.0 2.1249155346074992E-4 0.0 24-25 0.0 0.0 0.0 3.499860880529999E-4 0.0 26-27 0.0 0.0 0.0 6.499741635269999E-4 0.0 28-29 0.0 0.0 0.0 0.003037379264174249 0.0 30-31 0.0 0.0 0.0 0.010012102018944747 0.0 32-33 0.0 0.0 0.0 0.022261615100799742 0.0 34-35 0.0 0.0 0.0 0.043260780383979736 0.0 36-37 0.0 0.0 0.0 0.07909685589997797 0.0 38-39 0.0 0.0 0.0 0.1384069983218167 0.0 40-41 0.0 0.0 0.0 0.20424188138521493 0.0 42-43 0.0 0.0 0.0 0.27185169389516767 0.0 44-45 0.0 0.0 0.0 0.3558608545310324 0.0 46-47 0.0 0.0 0.0 0.46193163821738087 0.0 48-49 0.0 0.0 0.0 0.5871891592309206 0.0 50-51 0.0 0.0 0.0 0.7235712380432877 0.0 52-53 0.0 0.0 0.0 0.8582158859185347 0.0 54-55 0.0 0.0 0.0 1.0067099832781647 0.0 56-57 0.0 0.0 0.0 1.1905401760280028 0.0 58-59 0.0 0.0 0.0 1.416881178973136 0.0 60-61 0.0 0.0 0.0 1.6503843972202104 0.0 62-63 0.0 0.0 0.0 1.8987245257001035 0.0 64-65 0.0 0.0 0.0 2.1548518446391753 0.0 66-67 0.0 0.0 0.0 2.4466777445596537 0.0 68-69 0.0 0.0 0.0 2.7802644844867417 0.0 70-71 0.0 0.0 0.0 3.1514622293763823 0.0 72-73 0.0 0.0 0.0 3.5372843929453808 0.0 74-75 0.0 1.2499503144749997E-5 0.0 3.91003207622497 0.0 76-77 0.0 2.4999006289499993E-5 0.0 4.339627499806882 0.0 78-79 0.0 2.4999006289499993E-5 0.0 4.808921345376521 0.0 80-81 0.0 2.4999006289499993E-5 0.0 5.313613783852092 0.0 82-83 0.0 2.4999006289499993E-5 0.0 5.848555019937957 0.0 84-85 0.0 2.4999006289499993E-5 0.0 6.360259679677733 0.0 86-87 0.0 2.4999006289499993E-5 0.0 6.898188297015194 0.0 88 0.0 2.4999006289499993E-5 0.0 7.334658447326719 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4635 0.0 39.439304 94 GTACTAG 4530 0.0 35.80502 1 CTGTGCG 5910 0.0 32.679573 9 TAGGCAT 5270 0.0 32.637306 5 CAACGCA 6385 0.0 31.57477 5 TAGTACT 6270 0.0 31.555866 4 GCATCGT 4655 0.0 31.395168 8 TACCCCG 255 0.0 31.329433 5 CGTTAAC 805 0.0 30.368996 1 GGTATCA 5145 0.0 30.337214 1 CTAACGC 2115 0.0 30.220348 3 ACTGTGC 6615 0.0 29.978142 8 GAACCGT 440 0.0 29.903872 6 CTAGGCA 5960 0.0 29.885359 4 GTATCAA 7660 0.0 29.828434 1 TCTAACG 2170 0.0 29.671713 2 CGCCTAC 2210 0.0 29.130663 7 TAACGCC 2195 0.0 29.118559 4 GTACTGT 6840 0.0 28.923315 6 TACTGTG 7305 0.0 28.561752 7 >>END_MODULE