FastQCFastQC Report
Mon 16 Jan 2017
ERR1146676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000159
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86530.21631640142304345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76410.19101740705806944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.13111978798842747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69850.041.0152321
GTATCAA121350.038.6500131
GGGCACC41150.038.28781594
GTGGTAT17700.034.285521
CAACGCA142300.031.9388585
ATCAACG144900.031.3920563
GTACTAG45100.030.1450211
TACCCCG2500.030.0799965
TATCAAC160750.029.1768552
CTGTGCG56800.029.1293079
AACGCAG168300.027.8687256
TAGGCAT54800.027.7883175
TAGTACT61450.027.404564
ACTGTGC64450.026.8356348
CAGTACG7800.026.5151379
TATTCGC3400.026.2679
TCAACGC178650.025.9091854
GTACTGT66500.025.8660496
ACGCAGA178100.025.4386847
CTAGGCA61300.025.2462654
Produced by FastQC (version 0.11.5)