##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000159
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06249251592249	33.0	31.0	34.0	30.0	34.0
2	32.13686730952445	33.0	31.0	34.0	30.0	34.0
3	32.29600348386152	34.0	31.0	34.0	30.0	34.0
4	35.928162105556304	37.0	35.0	37.0	35.0	37.0
5	35.83337587330904	37.0	35.0	37.0	35.0	37.0
6	35.88175070040966	37.0	35.0	37.0	35.0	37.0
7	35.799774958945385	37.0	35.0	37.0	35.0	37.0
8	35.807580648669216	37.0	35.0	37.0	35.0	37.0
9	37.50953949580504	39.0	37.0	39.0	35.0	39.0
10-11	37.44586490187015	39.0	37.0	39.0	34.0	39.0
12-13	37.41751340384219	39.0	37.0	39.0	34.0	39.0
14-15	38.63370743512945	40.0	38.0	41.0	34.0	41.0
16-17	38.63240611185705	40.0	38.0	41.0	34.0	41.0
18-19	38.68401981021255	40.0	38.0	41.0	34.0	41.0
20-21	38.67968285760641	40.0	38.0	41.0	34.0	41.0
22-23	38.63775214935207	40.0	38.0	41.0	34.0	41.0
24-25	38.56348210158646	40.0	38.0	41.0	34.0	41.0
26-27	38.42170698714726	40.0	38.0	41.0	34.0	41.0
28-29	38.36198711101234	40.0	38.0	41.0	34.0	41.0
30-31	38.22845579388219	40.0	37.5	41.0	33.5	41.0
32-33	38.09483610526482	40.0	37.0	41.0	33.0	41.0
34-35	37.991136727315094	40.0	37.0	41.0	33.0	41.0
36-37	37.98898056302262	40.0	37.0	41.0	33.0	41.0
38-39	37.89652348819135	40.0	37.0	41.0	33.0	41.0
40-41	37.784588687599665	40.0	37.0	41.0	33.0	41.0
42-43	37.645439218791054	40.0	37.0	41.0	32.5	41.0
44-45	37.44368311359624	40.0	36.0	41.0	32.0	41.0
46-47	37.20535296221975	40.0	36.0	41.0	31.0	41.0
48-49	36.946409505222164	39.0	35.5	41.0	31.0	41.0
50-51	36.742785099292306	39.0	35.0	41.0	31.0	41.0
52-53	36.5612746893311	39.0	35.0	41.0	30.0	41.0
54-55	36.37317166642626	39.0	35.0	41.0	30.0	41.0
56-57	36.147766626276606	38.0	35.0	40.0	30.0	41.0
58-59	35.87441086716803	37.5	35.0	40.0	29.5	41.0
60-61	35.697534398007676	37.0	35.0	40.0	30.0	41.0
62-63	35.48876044677224	37.0	35.0	40.0	29.0	41.0
64-65	35.238973000823215	36.0	34.0	39.5	29.0	41.0
66-67	34.9907714918332	36.0	34.0	39.0	29.0	41.0
68-69	34.73877838355925	35.0	34.0	39.0	29.0	41.0
70-71	34.51445517540678	35.0	34.0	38.5	29.0	41.0
72-73	34.271486708403344	35.0	34.0	37.5	29.0	40.0
74-75	34.0143563043369	35.0	34.0	37.0	29.0	39.5
76-77	33.77064211697585	35.0	34.0	36.5	28.0	39.0
78-79	33.546026170459726	35.0	33.0	36.0	28.0	39.0
80-81	33.41338706786405	35.0	33.0	36.0	28.5	38.5
82-83	33.25788449909116	35.0	33.0	35.5	28.5	37.0
84-85	33.12835202300708	35.0	33.0	35.0	29.0	37.0
86-87	32.98621617290713	35.0	33.0	35.0	29.0	36.5
88-89	32.881895069670975	35.0	33.0	35.0	29.0	36.0
90-91	32.82377263003796	35.0	33.0	35.0	29.0	36.0
92-93	32.75955105784546	35.0	33.0	35.0	29.0	36.0
94-95	32.71136772313301	35.0	33.0	35.0	29.0	35.5
96-97	32.6238153283407	35.0	33.0	35.0	27.5	35.0
98-99	32.54606004411325	35.0	33.0	35.0	27.0	35.0
100	31.27500206866777	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42348446471235235
1101	2	0.46409827522829517
1101	3	0.4491049891692924
1101	4	0.3305231585224959
1101	5	0.285372873321144
1101	6	0.30025455695141545
1101	7	0.339584628807188
1101	8	0.3437074158604787
1101	9	0.4131613358258406
1101	10-11	0.4400008502421997
1101	12-13	0.43519897644799244
1101	14-15	0.5414283037992789
1101	16-17	0.5961399204948776
1101	18-19	0.6048320207126991
1101	20-21	0.6122633367006003
1101	22-23	0.5613359248197298
1101	24-25	0.5569495802524571
1101	26-27	0.664782996341863
1101	28-29	0.6371461103425204
1101	30-31	0.6355932477521549
1101	32-33	0.6563431147622865
1101	34-35	0.7163622461776171
1101	36-37	0.5412045344101415
1101	38-39	0.5954983902074744
1101	40-41	0.5897707525965927
1101	42-43	0.5215598649087667
1101	44-45	0.5422553923009445
1101	46-47	0.49509614643862676
1101	48-49	0.5197116257276946
1101	50-51	0.5592558055486521
1101	52-53	0.6345885670123437
1101	54-55	0.6011360521502098
1101	56-57	0.5570453676452161
1101	58-59	0.4855573406395024
1101	60-61	0.5190293370136558
1101	62-63	0.5285469124291993
1101	64-65	0.48342650984660906
1101	66-67	0.48774971356029084
1101	68-69	0.5237155296932698
1101	70-71	0.4112773321705916
1101	72-73	0.5119897508992182
1101	74-75	0.4377519273929735
1101	76-77	0.47744827620645935
1101	78-79	0.47299626141078477
1101	80-81	0.46018997493344926
1101	82-83	0.40208123339452584
1101	84-85	0.4495701009498987
1101	86-87	0.387137605090075
1101	88-89	0.4047811195086126
1101	90-91	0.42756352089381267
1101	92-93	0.42801828253922025
1101	94-95	0.38934732307689757
1101	96-97	0.3916042036321201
1101	98-99	0.39568916021657685
1101	100	0.65808544846724
1102	1	0.463720239988362
1102	2	0.48454372826775227
1102	3	0.3849318516539597
1102	4	0.2534560358274618
1102	5	0.299144213365345
1102	6	0.25508984219666786
1102	7	0.3470547707277092
1102	8	0.34487337521918704
1102	9	0.44988636263623505
1102	10-11	0.39543680029436246
1102	12-13	0.44480314318995795
1102	14-15	0.5560722306017354
1102	16-17	0.5467774302890263
1102	18-19	0.5936632156455843
1102	20-21	0.549669130171182
1102	22-23	0.6124922721892148
1102	24-25	0.610396821972941
1102	26-27	0.6852308858952654
1102	28-29	0.6704306162472164
1102	30-31	0.6997129218561753
1102	32-33	0.7255017212115362
1102	34-35	0.6724996790875082
1102	36-37	0.7485654762055489
1102	38-39	0.7448861082419569
1102	40-41	0.7354511456191091
1102	42-43	0.6881734567628328
1102	44-45	0.7258761662753344
1102	46-47	0.7687674448522444
1102	48-49	0.7152403894743316
1102	50-51	0.7553925319860753
1102	52-53	0.7656553905640919
1102	54-55	0.7154700403889578
1102	56-57	0.6825464355727817
1102	58-59	0.6296150969785472
1102	60-61	0.6072273880589663
1102	62-63	0.6491431029105001
1102	64-65	0.6077281367501115
1102	66-67	0.5987288669101645
1102	68-69	0.623775171824164
1102	70-71	0.6384159706302768
1102	72-73	0.5795591556985045
1102	74-75	0.6111815562652723
1102	76-77	0.570014938470301
1102	78-79	0.5960714591094103
1102	80-81	0.5815151727195058
1102	82-83	0.4969447588856468
1102	84-85	0.5167982305850387
1102	86-87	0.5680285274901351
1102	88-89	0.5821951831370669
1102	90-91	0.5397966861854115
1102	92-93	0.5468149034557754
1102	94-95	0.539817436202263
1102	96-97	0.5724714020805877
1102	98-99	0.5346098125168268
1102	100	0.8633334663514809
1103	1	0.3766190171508157
1103	2	0.43111478202148845
1103	3	0.2990287789224979
1103	4	0.26256964079254885
1103	5	0.2477947508334637
1103	6	0.25501820285608545
1103	7	0.2883272377170627
1103	8	0.2673096308890095
1103	9	0.3376442868729441
1103	10-11	0.38296128255888107
1103	12-13	0.34605694330748804
1103	14-15	0.4790820811227263
1103	16-17	0.45282035828802947
1103	18-19	0.4625641937972631
1103	20-21	0.45262711139778844
1103	22-23	0.44941286138706005
1103	24-25	0.4518228066202852
1103	26-27	0.4748055083288065
1103	28-29	0.5310934833639465
1103	30-31	0.48187838841128894
1103	32-33	0.532129108319559
1103	34-35	0.5307414022369557
1103	36-37	0.47558677249575254
1103	38-39	0.4519084943076592
1103	40-41	0.4352517702971923
1103	42-43	0.42419350729223737
1103	44-45	0.5603954641785336
1103	46-47	0.5773228971743976
1103	48-49	0.4976938872684826
1103	50-51	0.5170682203786114
1103	52-53	0.43822194475089304
1103	54-55	0.40657861117183103
1103	56-57	0.44433910734613136
1103	58-59	0.48352721797310494
1103	60-61	0.5477907863631017
1103	62-63	0.48433432110644503
1103	64-65	0.532226044459307
1103	66-67	0.5432699123108193
1103	68-69	0.527530799492979
1103	70-71	0.4883901387078282
1103	72-73	0.48301134341724605
1103	74-75	0.4128420346968156
1103	76-77	0.36607182698481466
1103	78-79	0.3818656436162158
1103	80-81	0.352874231082005
1103	82-83	0.3659445091082034
1103	84-85	0.40290158306275714
1103	86-87	0.4209215297356792
1103	88-89	0.4077146995536509
1103	90-91	0.330627085071157
1103	92-93	0.3190310881182299
1103	94-95	0.35695128642713314
1103	96-97	0.37562977471078085
1103	98-99	0.35370510233067165
1103	100	0.5910980766288922
1104	1	0.31298141542556834
1104	2	0.3248843352602364
1104	3	0.2798996042800894
1104	4	0.20029320208218593
1104	5	0.22754204294329128
1104	6	0.22222585686852625
1104	7	0.2511108850201964
1104	8	0.260829897622159
1104	9	0.31206431550953084
1104	10-11	0.35880193567854235
1104	12-13	0.3458545171499168
1104	14-15	0.44062117601696116
1104	16-17	0.4559974354263261
1104	18-19	0.43148008729556153
1104	20-21	0.4754908184947695
1104	22-23	0.4666102676698216
1104	24-25	0.5029699342823619
1104	26-27	0.5214993469666993
1104	28-29	0.5386363267402601
1104	30-31	0.5079370013622722
1104	32-33	0.5308368181737038
1104	34-35	0.5693923722574041
1104	36-37	0.5045464524553935
1104	38-39	0.46612607387948657
1104	40-41	0.4734761477746261
1104	42-43	0.5047504413617006
1104	44-45	0.48811635958892907
1104	46-47	0.5221804191549353
1104	48-49	0.4987096810469893
1104	50-51	0.5332642255188205
1104	52-53	0.4936221287518805
1104	54-55	0.5673445060013407
1104	56-57	0.5102258393758206
1104	58-59	0.47151018815675627
1104	60-61	0.49548621847757346
1104	62-63	0.5084799884994595
1104	64-65	0.48070220143705455
1104	66-67	0.4741981288445203
1104	68-69	0.5011637497603303
1104	70-71	0.4684728267785161
1104	72-73	0.3911534023646368
1104	74-75	0.419162433271687
1104	76-77	0.39997616617870335
1104	78-79	0.4304105675712293
1104	80-81	0.39429725304110974
1104	82-83	0.34734324814041884
1104	84-85	0.3620503874453149
1104	86-87	0.3707561159919166
1104	88-89	0.3525030353119831
1104	90-91	0.36187584246369653
1104	92-93	0.3237672594315697
1104	94-95	0.3610887802724463
1104	96-97	0.34161149381562694
1104	98-99	0.3836734553878287
1104	100	0.5821842590414192
1105	1	0.25002254047693384
1105	2	0.26543829596964486
1105	3	0.19377088469476433
1105	4	0.2395748584671864
1105	5	0.18497616415221785
1105	6	0.19342162824347042
1105	7	0.23035635891161377
1105	8	0.225771035999891
1105	9	0.29804880485954044
1105	10-11	0.2990628636522672
1105	12-13	0.27839261183935804
1105	14-15	0.4063287544204641
1105	16-17	0.3619395233082159
1105	18-19	0.3128105053689012
1105	20-21	0.36219507953344987
1105	22-23	0.34301951436876266
1105	24-25	0.3432496111455521
1105	26-27	0.4097603905050775
1105	28-29	0.38827205516541596
1105	30-31	0.4093696820040549
1105	32-33	0.37551801590932854
1105	34-35	0.29699382622033
1105	36-37	0.3189408190778167
1105	38-39	0.37949627048514856
1105	40-41	0.34810533447712544
1105	42-43	0.3410800542999439
1105	44-45	0.3666406475996382
1105	46-47	0.3842197829732754
1105	48-49	0.45291905399945165
1105	50-51	0.3771780722460889
1105	52-53	0.40715387293825245
1105	54-55	0.3543157369973784
1105	56-57	0.3676739376606051
1105	58-59	0.39537286542528705
1105	60-61	0.3235159557259024
1105	62-63	0.378781790960943
1105	64-65	0.3855521513243403
1105	66-67	0.3388250302821092
1105	68-69	0.3131365944969673
1105	70-71	0.34483400830038846
1105	72-73	0.3812280734391891
1105	74-75	0.35496991436489367
1105	76-77	0.2951265806844603
1105	78-79	0.2666755441251425
1105	80-81	0.17449563208205632
1105	82-83	0.2506152460276283
1105	84-85	0.27025852284960195
1105	86-87	0.2426884905435358
1105	88-89	0.2184662614605486
1105	90-91	0.25433534451540396
1105	92-93	0.23987981731801256
1105	94-95	0.2181503182748159
1105	96-97	0.27257233058698205
1105	98-99	0.24376019307758412
1105	100	0.4177060039357521
1106	1	0.17467892508238236
1106	2	0.22594844163373295
1106	3	0.1812457545549364
1106	4	0.20082827302261563
1106	5	0.16770346955152604
1106	6	0.2004353539714785
1106	7	0.2226174869367057
1106	8	0.11481124326173386
1106	9	0.1306427678197366
1106	10-11	0.21962371643178358
1106	12-13	0.17491618146428323
1106	14-15	0.21936527979054432
1106	16-17	0.24792448007051604
1106	18-19	0.2703024437433399
1106	20-21	0.30341095944707774
1106	22-23	0.28571102350105093
1106	24-25	0.28900771655840174
1106	26-27	0.3288587196453534
1106	28-29	0.28527385273936545
1106	30-31	0.32285758801359066
1106	32-33	0.3689610289150451
1106	34-35	0.36864443361530164
1106	36-37	0.24574912014963957
1106	38-39	0.29004420918627716
1106	40-41	0.31694990724572847
1106	42-43	0.3423749817346007
1106	44-45	0.34703521451390884
1106	46-47	0.30021941317514234
1106	48-49	0.45042852390095334
1106	50-51	0.39056451986586893
1106	52-53	0.3882740888989602
1106	54-55	0.30991502858342557
1106	56-57	0.2504512191485162
1106	58-59	0.19145336153366088
1106	60-61	0.21407836674676872
1106	62-63	0.2769051157093827
1106	64-65	0.2676940581448264
1106	66-67	0.2401103957810875
1106	68-69	0.2816709180859931
1106	70-71	0.2924925211032843
1106	72-73	0.2154301860891934
1106	74-75	0.18432084212624744
1106	76-77	0.25106395031181705
1106	78-79	0.26794573600831484
1106	80-81	0.2587664533828118
1106	82-83	0.20748371296733126
1106	84-85	0.24063861263473996
1106	86-87	0.18932470256894618
1106	88-89	0.2379514270813985
1106	90-91	0.22630035683749128
1106	92-93	0.24568636506223385
1106	94-95	0.20414353625223924
1106	96-97	0.18906578645416516
1106	98-99	0.21248267300036616
1106	100	0.3445164255099975
1107	1	0.10181613275265278
1107	2	0.1702482297738257
1107	3	0.16469816443942875
1107	4	0.1353242841354927
1107	5	0.05397277141717183
1107	6	0.03430007712987049
1107	7	0.10104501690786805
1107	8	0.12181356310693303
1107	9	0.06081312143763995
1107	10-11	0.15780449953535935
1107	12-13	0.06149743705373112
1107	14-15	0.12711126742656376
1107	16-17	0.11426228833926899
1107	18-19	0.14224298847900485
1107	20-21	0.18193019538516353
1107	22-23	0.14362399093602107
1107	24-25	0.13560738316584775
1107	26-27	0.14836319788298624
1107	28-29	0.12990492090196426
1107	30-31	0.15985962700369782
1107	32-33	0.2535866280753467
1107	34-35	0.15405052527087548
1107	36-37	0.05391800482652087
1107	38-39	0.16770920537671685
1107	40-41	0.22019650972755755
1107	42-43	0.2019365774015185
1107	44-45	0.19149211027448843
1107	46-47	0.2761193230057586
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2216	94-95	-1.2630327251875038
2216	96-97	-1.2223115377518603
2216	98-99	-1.3149513523908745
2216	100	-0.8374807359478673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	19.0
13	62.0
14	236.0
15	708.0
16	1633.0
17	3355.0
18	5993.0
19	9340.0
20	13138.0
21	18616.0
22	25338.0
23	29920.0
24	29953.0
25	29285.0
26	31176.0
27	38361.0
28	48062.0
29	60692.0
30	76188.0
31	95276.0
32	119891.0
33	154233.0
34	218581.0
35	316330.0
36	525934.0
37	975249.0
38	866698.0
39	305261.0
40	626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.671687800409934	22.333987224007846	13.201850226453498	25.792474749128726
2	14.154705701762719	21.53343686780555	38.594249536744904	25.71760789368683
3	17.259413938295953	26.93997913582935	29.733568090668395	26.0670388352063
4	11.679800007605795	17.52125119340938	38.72796070605802	32.07098809292681
5	12.388071679125439	38.95649317386305	35.42113698698973	13.23429816002178
6	29.85477819995565	39.13828882216373	17.435140670728675	13.571792307151945
7	25.99464821139565	32.44969323711713	23.877431841442263	17.678226710044953
8	26.64104101861951	34.641048018341266	19.638769358917983	19.079141604121236
9	26.06748881731951	14.115588905341012	21.427398260919126	38.389524016420346
10-11	25.578283263240287	26.044339737495438	27.493032151971956	20.88434484729232
12-13	25.70755916820463	23.744466603377255	28.202332791894293	22.345641436523824
14-15	23.011255001059432	24.741751259913833	25.82304890709546	26.42394483193128
16-17	21.642377215505686	27.129584099032062	27.181644529629946	24.046394155832306
18-19	21.047443625976776	27.32063333498327	29.729000704472174	21.902922334567783
20-21	22.480943882480673	26.604242481361368	29.04737036702791	21.867443269130053
22-23	22.771769822149572	26.216720385364685	28.84064108451689	22.170868707968857
24-25	22.296301222026425	26.280080366805418	29.63100966736572	21.792608743802433
26-27	22.30186350092584	26.453873458530026	28.551927560879452	22.69233547966468
28-29	21.557730580209437	26.9172550391122	29.088193744298664	22.436820636379704
30-31	22.444007850687935	26.58253084439893	29.021571392537147	21.951889912375982
32-33	22.90158966181094	26.740987045764932	28.244314788487156	22.113108503936967
34-35	22.68322334187216	26.625041654594227	29.022233866203816	21.669501137329792
36-37	22.48246882186433	26.087025540734754	28.629474478389483	22.80103115901143
38-39	22.498680677443073	26.275830535736205	29.350920800898166	21.87456798592256
40-41	22.047973593049676	25.585495476554808	29.800790418580863	22.565740511814656
42-43	22.327962463492078	25.682354126423473	28.943037014278683	23.046646395805766
44-45	22.482831307455527	26.65597792487749	28.632324365106488	22.2288664025605
46-47	22.527054549581653	26.571631277656714	27.8818541962957	23.019459976465935
48-49	23.48576644078398	26.380538873579773	27.77487094887978	22.358823736756463
50-51	22.764695103369643	26.25853122338387	27.66561279189152	23.31116088135497
52-53	22.132182745735857	26.88279390894212	27.537067901550916	23.44795544377111
54-55	22.298438637064177	26.842195522727973	27.29920235670632	23.56016348350153
56-57	23.345309523946423	26.299804582767834	27.069773976484434	23.285111916801306
58-59	22.18654863132906	26.714807265113027	28.03258021308158	23.066063890476325
60-61	22.424108641681492	27.323313898272545	27.16528268001347	23.087294780032494
62-63	22.828555064936168	26.587393151122242	26.739824591972468	23.844227191969118
64-65	21.949749447208923	26.793999232529742	27.303079505669153	23.953171814592185
66-67	22.597292926176884	26.411137883721597	27.217157758728767	23.77441143137275
68-69	22.17774343469847	26.227007476452812	27.654488234092696	23.940760854756025
70-71	22.154427211812212	26.00186418918083	27.582162631944136	24.261545967062826
72-73	22.69005185695445	25.940351815318397	28.02966697278107	23.339929354946083
74-75	22.73679455428992	26.359655707007217	27.535514698924874	23.368035039777986
76-77	22.462468618383923	26.36740724259888	27.111978855806125	24.058145283211076
78-79	22.235796686030902	26.39104505372038	27.221999731010627	24.151158529238096
80-81	21.844556678872014	26.902180638319628	27.71052350669061	23.54273917611775
82-83	22.62446317758869	25.780687717663227	28.753607044119995	22.84124206062809
84-85	22.855941476326315	26.324166114396952	27.307027045674936	23.512865363601797
86-87	21.5193696050582	26.86024480526899	27.85745516615715	23.762930423515666
88-89	22.439220540983495	25.78616250004062	27.640876275168065	24.13374068380782
90-91	22.089684435043708	25.764200873015298	27.816556791867526	24.329557900073475
92-93	22.47918145253726	26.036365054489085	27.512868863462675	23.97158462951098
94-95	21.31511522416984	26.015890868337983	28.13669406641086	24.53229984108132
96-97	21.675088415235493	25.905982737186196	27.677512318885324	24.741416528692984
98-99	21.111210829369533	26.519783338612292	27.603352766727525	24.765653065290653
100	21.86836751718364	25.849089121308666	26.720158845657522	25.562384515850173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	652.0
1	404.0
2	220.0
3	285.5
4	197.0
5	153.0
6	356.5
7	752.5
8	1126.5
9	1170.5
10	884.0
11	645.0
12	636.0
13	719.5
14	883.0
15	1269.5
16	1709.5
17	2218.5
18	2949.5
19	3779.5
20	4782.5
21	5924.5
22	7197.0
23	8499.0
24	9749.0
25	11228.0
26	13507.0
27	16064.0
28	19574.5
29	26416.0
30	36203.5
31	44959.0
32	57329.5
33	70596.5
34	79431.0
35	90532.5
36	101092.0
37	117762.5
38	125520.0
39	119179.5
40	111784.0
41	103662.0
42	102191.0
43	108020.0
44	119078.5
45	142861.5
46	164811.5
47	180154.0
48	192579.5
49	188913.5
50	178375.5
51	180046.5
52	181242.0
53	172497.0
54	164394.5
55	141013.5
56	120030.5
57	99483.0
58	78478.0
59	77180.0
60	64339.0
61	42402.5
62	30616.5
63	24983.5
64	18270.0
65	8832.0
66	4549.5
67	3224.0
68	2491.0
69	1833.5
70	1409.5
71	1144.0
72	866.5
73	665.0
74	515.0
75	390.5
76	258.5
77	148.0
78	86.0
79	46.5
80	24.5
81	9.5
82	7.0
83	6.0
84	5.0
85	2.5
86	1.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005074798276768498
3	0.0
4	0.08017181317042647
5	0.004149835044056999
6	4.999801257899999E-5
7	2.4999006289499993E-5
8	0.0
9	0.0
10-11	0.0
12-13	4.124836037767499E-4
14-15	0.009537120899444248
16-17	0.0
18-19	2.4999006289499993E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.4999006289499993E-5
60-61	0.0
62-63	0.0
64-65	9.999602515799997E-5
66-67	2.374905597502499E-4
68-69	0.0
70-71	7.124716792507497E-4
72-73	5.124796289347499E-4
74-75	4.8748062264524985E-4
76-77	1.6249354088174994E-4
78-79	2.4999006289499993E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.057897698566481984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4000159.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.39048848892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51017614583093	50.88667656351941
2	15.403833106683745	20.761436753224313
3	4.894958624142234	9.896209584419905
4	1.9560665780119675	5.272811288363065
5	0.9227775911947191	3.109321631862052
6	0.46018830914860853	1.860738897024892
7	0.27733106297633353	1.3082633064988585
8	0.15450884906223405	0.8329941451331809
9	0.10293207199300318	0.624297835150665
>10	0.3004568928104346	3.1216506030576374
>50	0.010687993852376669	0.487071557851173
>100	0.0052264139284639854	0.6771201235300381
>500	5.541155303642809E-4	0.2664869206328528
>1k	1.511224173077619E-4	0.16372820608745026
>5k	1.511224173077619E-4	0.7311925836445349
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8653	0.21631640142304345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7641	0.19101740705806944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5245	0.13111978798842747	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.999801257899999E-5	0.0
5	0.0	0.0	0.0	4.999801257899999E-5	0.0
6	0.0	0.0	0.0	4.999801257899999E-5	0.0
7	0.0	0.0	0.0	4.999801257899999E-5	0.0
8	0.0	0.0	0.0	4.999801257899999E-5	0.0
9	0.0	0.0	0.0	9.999602515799997E-5	0.0
10-11	0.0	0.0	0.0	1.4999403773699997E-4	0.0
12-13	0.0	0.0	0.0	1.4999403773699997E-4	0.0
14-15	0.0	0.0	0.0	1.4999403773699997E-4	0.0
16-17	0.0	0.0	0.0	1.4999403773699997E-4	0.0
18-19	0.0	0.0	0.0	1.4999403773699997E-4	0.0
20-21	0.0	0.0	0.0	1.6249354088174997E-4	0.0
22-23	0.0	0.0	0.0	2.3749055975024993E-4	0.0
24-25	0.0	0.0	0.0	3.749850943424999E-4	0.0
26-27	0.0	0.0	0.0	7.249711823954998E-4	0.0
28-29	0.0	0.0	0.0	0.003249870817634999	0.0
30-31	0.0	0.0	0.0	0.010524581647879496	0.0
32-33	0.0	0.0	0.0	0.023011585289484743	0.0
34-35	0.0	0.0	0.0	0.04478571976763923	0.0
36-37	0.0	0.0	0.0	0.08200924013270472	0.0
38-39	0.0	0.0	0.0	0.14326930504512447	0.0
40-41	0.0	0.0	0.0	0.21077912152991918	0.0
42-43	0.0	0.0	0.0	0.2804138535493214	0.0
44-45	0.0	0.0	0.0	0.3667229227638201	0.0
46-47	0.0	0.0	0.0	0.4752936070791186	0.0
48-49	0.0	0.0	0.0	0.6034760118285298	0.0
50-51	0.0	0.0	0.0	0.742857971395637	0.0
52-53	0.0	0.0	0.0	0.8801275149312815	0.0
54-55	0.0	0.0	0.0	1.0316464920519408	0.0
56-57	0.0	0.0	0.0	1.2203014930156526	0.0
58-59	0.0	0.0	0.0	1.4521922753570546	0.0
60-61	0.0	0.0	0.0	1.6916827556104646	0.0
62-63	0.0	0.0	0.0	1.9455351649771921	0.0
64-65	0.0	0.0	0.0	2.2082872205829815	0.0
66-67	0.0	0.0	0.0	2.506712858163888	0.0
68-69	0.0	0.0	0.0	2.8476868044495234	0.0
70-71	0.0	0.0	0.0	3.2258717715970793	0.0
72-73	0.0	0.0	0.0	3.6186811574239925	0.0
74-75	0.0	0.0	0.0	3.999166033150182	0.0
76-77	0.0	0.0	0.0	4.438161083096947	0.0
78-79	0.0	0.0	0.0	4.917929512301886	0.0
80-81	0.0	0.0	0.0	5.433059035903323	0.0
82-83	0.0	0.0	0.0	5.9817872239578485	0.0
84-85	0.0	0.0	0.0	6.506766356037348	0.0
86-87	0.0	0.0	0.0	7.057894448695665	0.0
88	0.0	0.0	0.0	7.504401700032424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6985	0.0	41.015232	1
GTATCAA	12135	0.0	38.650013	1
GGGCACC	4115	0.0	38.287815	94
GTGGTAT	1770	0.0	34.28552	1
CAACGCA	14230	0.0	31.938858	5
ATCAACG	14490	0.0	31.392056	3
GTACTAG	4510	0.0	30.145021	1
TACCCCG	250	0.0	30.079996	5
TATCAAC	16075	0.0	29.176855	2
CTGTGCG	5680	0.0	29.129307	9
AACGCAG	16830	0.0	27.868725	6
TAGGCAT	5480	0.0	27.788317	5
TAGTACT	6145	0.0	27.40456	4
ACTGTGC	6445	0.0	26.835634	8
CAGTACG	780	0.0	26.515137	9
TATTCGC	340	0.0	26.267	9
TCAACGC	17865	0.0	25.909185	4
GTACTGT	6650	0.0	25.866049	6
ACGCAGA	17810	0.0	25.438684	7
CTAGGCA	6130	0.0	25.246265	4
>>END_MODULE