FastQCFastQC Report
Mon 16 Jan 2017
ERR1146675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4355798
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC42600.034.8312394
CAACGCA62850.033.125165
GTATCAA71300.032.8408051
GTACTAG37300.032.522571
CTGTGCG52050.030.9676349
TAGTACT52500.030.6154964
ATCAACG68150.030.4814723
GGTATCA45200.030.2711241
TAGGCAT45600.030.1968985
TCTAACG18150.029.519752
CTAACGC18800.028.4987953
CTAGTAC59800.028.0573433
GTACTGT58700.028.0204146
GTACATG161400.027.9084661
AACGCAG79900.027.5848526
TACATGG162700.027.2400952
TAACGCC19250.027.0998424
CTAGGCA51850.027.0110844
ACATGGG158650.026.839083
GCATCGT41500.026.8373648
Produced by FastQC (version 0.11.5)