##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146675_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4355798
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216734109341157	31.0	31.0	34.0	28.0	34.0
2	31.334083674219972	31.0	31.0	34.0	28.0	34.0
3	31.471306061484025	33.0	31.0	34.0	28.0	34.0
4	34.889082781157434	37.0	35.0	37.0	32.0	37.0
5	35.04656506109787	37.0	35.0	37.0	32.0	37.0
6	35.12463112384918	37.0	35.0	37.0	32.0	37.0
7	35.055770033412934	37.0	35.0	37.0	32.0	37.0
8	35.02841385206568	37.0	35.0	37.0	32.0	37.0
9	36.52484825972187	39.0	37.0	39.0	32.0	39.0
10-11	36.36391621007218	39.0	37.0	39.0	31.0	39.0
12-13	36.29866134747296	39.0	37.0	39.0	31.0	39.0
14-15	37.46781267175383	40.0	37.0	41.0	31.0	41.0
16-17	37.480049350314225	40.0	37.0	41.0	31.0	41.0
18-19	37.43852974816555	40.0	37.0	41.0	31.0	41.0
20-21	37.496012211769234	40.0	37.0	41.0	31.0	41.0
22-23	37.435770322682544	40.0	37.0	41.0	31.0	41.0
24-25	37.39402619221553	40.0	37.0	41.0	30.5	41.0
26-27	37.43550653634535	40.0	37.0	41.0	31.0	41.0
28-29	37.403213142574565	40.0	37.0	41.0	30.5	41.0
30-31	37.25643865946033	40.0	36.0	41.0	30.0	41.0
32-33	37.23977787767018	40.0	36.0	41.0	30.0	41.0
34-35	37.16946458490499	40.0	36.0	41.0	30.0	41.0
36-37	37.05492908532489	40.0	36.0	41.0	30.0	41.0
38-39	36.95635472535687	40.0	36.0	41.0	30.0	41.0
40-41	36.8715051754007	40.0	35.5	41.0	30.0	41.0
42-43	36.7022350439575	39.0	35.0	41.0	29.5	41.0
44-45	36.573604423345614	39.0	35.0	41.0	29.0	41.0
46-47	36.57740969622559	39.0	35.0	41.0	29.0	41.0
48-49	36.44641670711084	39.0	35.0	41.0	29.0	41.0
50-51	36.284876158168956	39.0	35.0	41.0	28.5	41.0
52-53	36.084695617198044	39.0	35.0	40.5	28.0	41.0
54-55	35.870893117633095	38.0	34.0	40.0	28.0	41.0
56-57	35.64490708705959	38.0	34.0	40.0	28.0	41.0
58-59	35.37077982036816	37.5	34.0	40.0	27.5	41.0
60-61	35.11168252063112	37.0	34.0	40.0	27.0	41.0
62-63	34.80647461154075	36.5	33.0	40.0	26.5	41.0
64-65	34.65384838782698	36.0	33.0	39.0	26.5	41.0
66-67	34.38378398171817	35.5	33.0	39.0	26.0	41.0
68-69	34.25266828259713	35.0	33.0	39.0	26.0	41.0
70-71	34.12487539596648	35.0	33.0	38.5	26.0	41.0
72-73	33.87702930668502	35.0	33.0	37.5	26.0	40.0
74-75	33.61118662986668	35.0	33.0	37.0	26.0	39.5
76-77	33.33745871594597	35.0	33.0	36.5	26.0	39.0
78-79	33.07729812080358	35.0	33.0	36.0	26.0	39.0
80-81	32.86964420755967	35.0	33.0	36.0	26.0	38.0
82-83	32.625789120615785	35.0	32.0	35.0	25.0	37.0
84-85	32.44088936171971	35.0	32.0	35.0	25.0	37.0
86-87	32.26313823551965	35.0	32.0	35.0	25.0	36.0
88-89	32.12677711409023	35.0	32.0	35.0	25.0	36.0
90-91	31.98586022584151	35.0	32.0	35.0	25.0	36.0
92-93	31.840510625148365	35.0	32.0	35.0	24.0	36.0
94-95	31.756379657642526	35.0	32.0	35.0	24.0	35.0
96-97	31.651325887931442	35.0	32.0	35.0	24.0	35.0
98-99	31.551303113688927	35.0	31.0	35.0	24.0	35.0
100	30.58867307437122	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28721852140730064
1101	2	0.4155191508030711
1101	3	0.46053261516994226
1101	4	0.5336365354519401
1101	5	0.44942237105825456
1101	6	0.4658715589201634
1101	7	0.44228523870394554
1101	8	0.4412104972091768
1101	9	0.5969361749063751
1101	10-11	0.6673454683464612
1101	12-13	0.5361982324788457
1101	14-15	0.73608767708469
1101	16-17	0.678377168245504
1101	18-19	0.7443248728970175
1101	20-21	0.6988126949934141
1101	22-23	0.667660652562958
1101	24-25	0.7284681802672566
1101	26-27	0.6849723370717342
1101	28-29	0.6703908074136393
1101	30-31	0.7689883324958942
1101	32-33	0.787385209206704
1101	34-35	0.7752928309955607
1101	36-37	0.7632438439905869
1101	38-39	0.7551557075300224
1101	40-41	0.707792226509298
1101	42-43	0.7278509877509762
1101	44-45	0.7026742223106908
1101	46-47	0.7690331016730241
1101	48-49	0.7118631760038525
1101	50-51	0.7078965287312045
1101	52-53	0.6273990307522581
1101	54-55	0.6219162328256189
1101	56-57	0.5481882684900228
1101	58-59	0.5475520499255069
1101	60-61	0.6264986165582513
1101	62-63	0.5550861532154201
1101	64-65	0.5604774404730506
1101	66-67	0.5902679846498344
1101	68-69	0.5600329227804863
1101	70-71	0.6114211117592703
1101	72-73	0.6232591129924074
1101	74-75	0.5021022054635793
1101	76-77	0.5755151351777101
1101	78-79	0.5753028053073876
1101	80-81	0.5031303072761304
1101	82-83	0.5402260954692153
1101	84-85	0.5285888104081948
1101	86-87	0.5152956940528455
1101	88-89	0.5145714807858539
1101	90-91	0.5466201738237473
1101	92-93	0.4544015919473381
1101	94-95	0.49098154675750294
1101	96-97	0.4644196857677798
1101	98-99	0.4067486244988956
1101	100	0.5055616046352007
1102	1	0.3299354668840273
1102	2	0.36180581984854143
1102	3	0.38003838291415803
1102	4	0.5326261191743598
1102	5	0.3588895741916218
1102	6	0.40810321920485393
1102	7	0.40563997073981994
1102	8	0.4764940066827492
1102	9	0.5364027775754678
1102	10-11	0.6655746222650265
1102	12-13	0.5385167647980751
1102	14-15	0.7109700691200658
1102	16-17	0.690546005260174
1102	18-19	0.7697858850635555
1102	20-21	0.67540651977599
1102	22-23	0.7264911639009028
1102	24-25	0.7322015900067882
1102	26-27	0.6880510435226839
1102	28-29	0.6860298336174111
1102	30-31	0.7069525935247754
1102	32-33	0.6121744951904091
1102	34-35	0.6351382121761731
1102	36-37	0.6868057162333159
1102	38-39	0.6000239002655121
1102	40-41	0.5614542866783552
1102	42-43	0.6990271686920408
1102	44-45	0.6324991487465113
1102	46-47	0.5895911720954459
1102	48-49	0.5471616337749197
1102	50-51	0.5407227830550525
1102	52-53	0.4767627309874456
1102	54-55	0.44995227490213807
1102	56-57	0.5224738000136497
1102	58-59	0.4701786247988977
1102	60-61	0.5235430028479726
1102	62-63	0.5285931143884639
1102	64-65	0.48825146244069373
1102	66-67	0.5249578161209882
1102	68-69	0.39737217988297147
1102	70-71	0.44993520667188136
1102	72-73	0.4586748025248184
1102	74-75	0.48858332217712785
1102	76-77	0.41549385400318783
1102	78-79	0.4260324521861847
1102	80-81	0.42605492280976165
1102	82-83	0.38576722943892605
1102	84-85	0.354492279031696
1102	86-87	0.3975118270035267
1102	88-89	0.40103237992095586
1102	90-91	0.38854783263497694
1102	92-93	0.26693433319920246
1102	94-95	0.32796643010599524
1102	96-97	0.36579429360934057
1102	98-99	0.3263007297738696
1102	100	0.41002525538686996
1103	1	0.24011402633481183
1103	2	0.33813043593576353
1103	3	0.34132213778670817
1103	4	0.4489496076688013
1103	5	0.28166532256121
1103	6	0.3478337416439743
1103	7	0.35942192975924314
1103	8	0.349549901485382
1103	9	0.4495441678697887
1103	10-11	0.5461813361102656
1103	12-13	0.4832463215100731
1103	14-15	0.6172707338436183
1103	16-17	0.5961627935193974
1103	18-19	0.6003240805338521
1103	20-21	0.5543900253088694
1103	22-23	0.6143183220870938
1103	24-25	0.5383246921438243
1103	26-27	0.5044449732895302
1103	28-29	0.4743527166724846
1103	30-31	0.5142077569798573
1103	32-33	0.5365022880278403
1103	34-35	0.595810894477431
1103	36-37	0.6409167070160962
1103	38-39	0.6529217043736466
1103	40-41	0.6149826419050584
1103	42-43	0.6256719579037409
1103	44-45	0.5163547274048312
1103	46-47	0.5551397424176159
1103	48-49	0.597847131272708
1103	50-51	0.6269524579218455
1103	52-53	0.6571068873258099
1103	54-55	0.5942754038218894
1103	56-57	0.565170193328278
1103	58-59	0.5635720197197784
1103	60-61	0.6428650895049941
1103	62-63	0.5799395395556886
1103	64-65	0.5846761305198314
1103	66-67	0.5495062881828616
1103	68-69	0.5455579677168885
1103	70-71	0.4542508636256457
1103	72-73	0.45391640377692255
1103	74-75	0.4900524610038204
1103	76-77	0.5017270941928587
1103	78-79	0.4935302415671572
1103	80-81	0.4398362505293463
1103	82-83	0.4695101747939816
1103	84-85	0.47332646313821414
1103	86-87	0.49544005115946277
1103	88-89	0.5613716760111771
1103	90-91	0.582752276710103
1103	92-93	0.5243030355495506
1103	94-95	0.5389548692851278
1103	96-97	0.5326941997367669
1103	98-99	0.45863377295330565
1103	100	0.6477275654736481
1104	1	0.19890387624108996
1104	2	0.2474008098186431
1104	3	0.23010205283616614
1104	4	0.3974196433851418
1104	5	0.3263060513300857
1104	6	0.3462879527360272
1104	7	0.36014164760783984
1104	8	0.4103471728287289
1104	9	0.4511517058186314
1104	10-11	0.49862086789209314
1104	12-13	0.39181855761933804
1104	14-15	0.493640948270027
1104	16-17	0.48623103789927313
1104	18-19	0.4277218847251234
1104	20-21	0.423976532277031
1104	22-23	0.4594154321953141
1104	24-25	0.46189908007193026
1104	26-27	0.4400054482923039
1104	28-29	0.40490646529310936
1104	30-31	0.31031301988682003
1104	32-33	0.4019336965330851
1104	34-35	0.47654023820844316
1104	36-37	0.3842395705176287
1104	38-39	0.36651627832451794
1104	40-41	0.41197132408976245
1104	42-43	0.4397219069642233
1104	44-45	0.3958142504730233
1104	46-47	0.34829472370861936
1104	48-49	0.30952378322228924
1104	50-51	0.2078900361968934
1104	52-53	0.26469933072847596
1104	54-55	0.3938699803399004
1104	56-57	0.35766083378731395
1104	58-59	0.3193370972127596
1104	60-61	0.37562036334067983
1104	62-63	0.40574284781899905
1104	64-65	0.37792682970050606
1104	66-67	0.3287024564050327
1104	68-69	0.30451200268435485
1104	70-71	0.36796560982837434
1104	72-73	0.3531800407339958
1104	74-75	0.26995450282043265
1104	76-77	0.25398197708879877
1104	78-79	0.23528018921570748
1104	80-81	0.24372026520168788
1104	82-83	0.27454354907330725
1104	84-85	0.35823357415226553
1104	86-87	0.24266560761596878
1104	88-89	0.22881609637335032
1104	90-91	0.23786035495160007
1104	92-93	0.14763231600039717
1104	94-95	0.30309348575087824
1104	96-97	0.2486448984223486
1104	98-99	0.2738684371870477
1104	100	0.38413000950710696
1105	1	0.05761178751859575
1105	2	0.22832907717755901
1105	3	0.2191129746517504
1105	4	0.2947315309557723
1105	5	0.15697075800535742
1105	6	0.18823850833868505
1105	7	0.2554054909302792
1105	8	0.20616618398426567
1105	9	0.30608978617937055
1105	10-11	0.31501462505317335
1105	12-13	0.2455947614975713
1105	14-15	0.42007468795099356
1105	16-17	0.3502099590234806
1105	18-19	0.3643578660579152
1105	20-21	0.3493086600471571
1105	22-23	0.35637694276925913
1105	24-25	0.3456245339789774
1105	26-27	0.3123017635519787
1105	28-29	0.41152476539463834
1105	30-31	0.33675089044260176
1105	32-33	0.3100777841641644
1105	34-35	0.3409993055873599
1105	36-37	0.36472084732753984
1105	38-39	0.28707921600701525
1105	40-41	0.3298968205013324
1105	42-43	0.3163741889464262
1105	44-45	0.339038773302633
1105	46-47	0.32674290351473445
1105	48-49	0.1714073045104243
1105	50-51	0.28934986833117904
1105	52-53	0.3328870969253117
1105	54-55	0.3521045939183054
1105	56-57	0.31592208063751315
1105	58-59	0.4008995897391401
1105	60-61	0.4456716432596295
1105	62-63	0.3805852087444066
1105	64-65	0.40263265434269613
1105	66-67	0.29795373262793134
1105	68-69	0.2857352063517027
1105	70-71	0.2850990381718219
1105	72-73	0.26778820008140514
1105	74-75	0.18047071813467142
1105	76-77	0.17034392379616747
1105	78-79	0.22123344836722936
1105	80-81	0.21607832616905398
1105	82-83	0.2997003728436596
1105	84-85	0.17447520913077597
1105	86-87	0.12850290332790593
1105	88-89	0.15357003755403298
1105	90-91	0.12128197214560288
1105	92-93	0.2056589454674551
1105	94-95	0.24907081540121112
1105	96-97	0.15485365761626468
1105	98-99	0.09660098097975833
1105	100	0.2743472554746127
1106	1	0.002950280908400771
1106	2	0.18344853879963807
1106	3	0.15221460160312006
1106	4	0.2541374929860609
1106	5	0.12338871355851211
1106	6	0.1512884896199509
1106	7	0.16822354030264108
1106	8	0.13567485098471366
1106	9	0.2939171949198922
1106	10-11	0.2650663383813665
1106	12-13	0.20669377684694012
1106	14-15	0.3376656091383481
1106	16-17	0.26397357521270237
1106	18-19	0.26907237149988816
1106	20-21	0.22847696178416044
1106	22-23	0.3112878596264963
1106	24-25	0.34445612398904046
1106	26-27	0.3597345903517919
1106	28-29	0.30588104383922143
1106	30-31	0.4081404296008486
1106	32-33	0.2674264217558928
1106	34-35	0.3184204951968752
1106	36-37	0.30631225565309705
1106	38-39	0.29536372275703116
1106	40-41	0.29268645347879385
1106	42-43	0.253754039467168
1106	44-45	0.39266533676816806
1106	46-47	0.36051594673020304
1106	48-49	0.29389231824501394
1106	50-51	0.2881807141023316
1106	52-53	0.2668649862637551
1106	54-55	0.3375164512228963
1106	56-57	0.28192937417887975
1106	58-59	0.27715949601014245
1106	60-61	0.4082633086858962
1106	62-63	0.3242166210392341
1106	64-65	0.2470255358102449
1106	66-67	0.3349762582590685
1106	68-69	0.2970587563262157
1106	70-71	0.26758558548963407
1106	72-73	0.20882528573202563
1106	74-75	0.19065849186799966
1106	76-77	0.19333373448884572
1106	78-79	0.17325121537984955
1106	80-81	0.1716809116099327
1106	82-83	0.14735543389380013
1106	84-85	0.12483711221733529
1106	86-87	0.16206805580666384
1106	88-89	0.14946948411091654
1106	90-91	0.1692822472884714
1106	92-93	0.16300388441731428
1106	94-95	0.08905428427623718
1106	96-97	0.1340094426078977
1106	98-99	0.10274728932921562
1106	100	0.09282869235187619
1107	1	0.09433491731872934
1107	2	0.14988650845512552
1107	3	0.13747621996236603
1107	4	0.23832175554565538
1107	5	0.19334367953500475
1107	6	0.22876920743098594
1107	7	0.16328699132400715
1107	8	0.2269908893132282
1107	9	0.2591371738907853
1107	10-11	0.38682466019718476
1107	12-13	0.2586230648332375
1107	14-15	0.365434297817238
1107	16-17	0.2675918044034802
1107	18-19	0.2274627854028921
1107	20-21	0.23140703891291992
1107	22-23	0.3047852927091981
1107	24-25	0.3963758783408977
1107	26-27	0.2650330867929327
1107	28-29	0.2798560293458934
1107	30-31	0.4188980614680986
1107	32-33	0.35868372628332423
1107	34-35	0.26984031237152806
1107	36-37	0.30513810866539615
1107	38-39	0.3154002261064761
1107	40-41	0.24404585055631856
1107	42-43	0.3094473826758133
1107	44-45	0.292386425608413
1107	46-47	0.31841064470686575
1107	48-49	0.2554599673720048
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2216	3	-0.697087705158637
2216	4	-0.5890503492569152
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2216	80-81	-1.0907952934899647
2216	82-83	-1.029497352085933
2216	84-85	-1.0693946242760646
2216	86-87	-1.0236864712936864
2216	88-89	-1.0912655277476269
2216	90-91	-1.220286456530122
2216	92-93	-1.1727062379861053
2216	94-95	-1.1211912384230729
2216	96-97	-1.1530724736830251
2216	98-99	-1.197073504518869
2216	100	-0.838137213555818
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	11.0
3	19.0
4	33.0
5	27.0
6	45.0
7	149.0
8	473.0
9	768.0
10	1255.0
11	2406.0
12	4475.0
13	6703.0
14	8957.0
15	11790.0
16	14083.0
17	16426.0
18	19146.0
19	21791.0
20	24736.0
21	27925.0
22	31534.0
23	35709.0
24	41455.0
25	46763.0
26	52706.0
27	58207.0
28	67029.0
29	80233.0
30	93856.0
31	108499.0
32	133867.0
33	173089.0
34	242362.0
35	346195.0
36	558178.0
37	953284.0
38	882733.0
39	288352.0
40	528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6905949151534	19.140983123024878	11.715081908541492	24.453340053280233
2	16.130974192535206	19.399956468672595	39.39270583935103	25.07636349944116
3	19.258387129880635	25.018109482526846	28.530143707210247	27.19335968038227
4	12.48857736670074	15.736577315270189	38.69951425682396	33.07533106120511
5	13.626429787096288	38.09532993038207	34.6670092117537	13.611231070767932
6	31.872264643077465	37.01991852961171	17.425516444799943	13.68230038251089
7	28.344897711329892	31.46423782204763	22.280997511112293	17.909866955510186
8	26.794505640832554	34.775216151560926	19.59425952880567	18.836018678800848
9	26.305582818212603	15.264562563493294	20.395796385258652	38.03405823303545
10-11	25.876703110970507	25.597051641049738	27.427378661773243	21.09886658620651
12-13	25.922276457891797	23.27835548338078	27.469919597721145	23.329448461006276
14-15	23.155145220499612	24.84425328126471	24.634360242344098	27.366241255891584
16-17	22.801555544790787	27.608142113925588	25.734157863598494	23.85614447768513
18-19	22.291924755701224	27.946404875231075	27.194154736792804	22.567515632274894
20-21	23.633903570663165	27.10181771973407	26.564747204247617	22.69953150535515
22-23	23.815803958468237	26.776579604364393	26.337080142675756	23.070536294491614
24-25	23.36549356751313	26.80580332877881	27.00386375086768	22.824839352840385
26-27	23.338570604844595	27.03871612882563	26.17368815761727	23.449025108712508
28-29	22.72701496248416	27.380868471978893	26.552732821244796	23.339383744292153
30-31	23.279715435833587	27.22435142287823	26.527278959622535	22.96865418166565
32-33	23.47752507357598	27.265398034660066	25.885761588453672	23.371315303310286
34-35	23.583917029155575	27.12172383895356	26.577545650184742	22.71681348170612
36-37	23.286429365259853	26.74725823991705	26.333237629692114	23.63307476513098
38-39	23.41822604193237	26.91501644917722	26.752131826684057	22.91462568220635
40-41	23.206516280081573	26.306478913983934	27.203908114986557	23.283096690947932
42-43	23.12847422613423	26.491468295402974	26.6949034331746	23.685154045288197
44-45	23.21776493310239	27.100999577720835	26.682210703754976	22.999024785421792
46-47	23.232786417722075	26.982179213150133	26.270709392896464	23.514324976231336
48-49	23.712077382765486	26.811399601572628	26.32332549881208	23.153197516849808
50-51	23.369540321007555	26.65029317407448	26.513508073983356	23.466658430934604
52-53	23.116189137738218	26.988789150138587	26.354797449198685	23.54022426292451
54-55	22.948342959068583	26.926708476157934	26.427557157395466	23.697391407378017
56-57	23.684363702659304	26.623576640550816	26.188241012843317	23.50381864394657
58-59	22.928274859346935	26.885653456384578	26.80076334510666	23.385308339161824
60-61	22.872686192410978	27.27574880513788	26.39977681364743	23.451788188803715
62-63	23.168930578287917	26.849535299330324	26.124148328123436	23.857385794258324
64-65	22.597213725257664	26.995278296269603	26.4352267638139	23.97228121465883
66-67	23.01238595426564	26.750742873687884	26.41387090948772	23.823000262558764
68-69	22.777090217939953	26.67083067343147	26.61965494610879	23.932424162519784
70-71	22.64714455141293	26.441930676753067	26.688140212812193	24.22278455902181
72-73	23.08742007299576	26.446940086789944	27.04117144038911	23.424468399825184
74-75	23.15216611399041	26.665132145782543	26.67608426241685	23.506617477810195
76-77	23.02534194159871	26.55472615397756	26.479416460081616	23.940515444342118
78-79	22.921074876239118	26.542385914455203	26.463642294795036	24.07289691451065
80-81	22.58262713761535	26.84634333774397	26.91616504556779	23.65486447907289
82-83	23.150657616280316	26.174655895254357	27.651533110177002	23.023153378288324
84-85	23.20530823742051	26.441872329717242	26.84143893111543	23.51138050174682
86-87	22.308833505169375	26.954399142308365	27.113614867389686	23.623152485132575
88-89	23.127316617563864	26.024994214195974	26.94056326179753	23.90712590644263
90-91	22.809274548375782	26.128014391817693	27.017147685467148	24.045563374339377
92-93	23.02109562426687	26.226537170760423	27.06649572386615	23.685871481106556
94-95	22.22459597889701	26.184236401062428	27.55192203690211	24.039245583138456
96-97	22.51882308561074	26.024670852865494	27.242359697324463	24.214146364199298
98-99	22.00240826068544	26.466908916247107	27.16585325589888	24.36482956716858
100	22.705324239780687	25.887530584858453	26.616273101206822	24.790872074154038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	614.0
1	522.0
2	480.5
3	555.5
4	519.0
5	442.0
6	417.5
7	396.5
8	409.0
9	421.5
10	414.0
11	402.0
12	399.5
13	438.0
14	489.5
15	598.5
16	737.0
17	862.0
18	1067.5
19	1364.0
20	1817.5
21	2387.5
22	3274.0
23	4719.0
24	6304.5
25	8311.5
26	11172.0
27	14575.5
28	19158.5
29	26386.5
30	36262.5
31	46122.5
32	58995.5
33	70638.0
34	78269.5
35	87833.5
36	98410.0
37	114343.0
38	122080.0
39	119312.0
40	116109.0
41	112146.5
42	113899.0
43	122611.0
44	134282.0
45	153741.5
46	172487.5
47	185296.0
48	194470.0
49	194179.0
50	188591.5
51	191894.5
52	194612.0
53	189041.0
54	180954.0
55	160626.5
56	143658.5
57	124442.0
58	104304.5
59	97269.0
60	82419.5
61	62581.5
62	49366.5
63	39790.0
64	29561.0
65	18995.0
66	13403.5
67	10226.0
68	7786.0
69	5966.5
70	4575.5
71	3499.5
72	2674.0
73	2273.0
74	1878.0
75	1490.5
76	1076.5
77	679.0
78	440.5
79	259.5
80	161.5
81	98.5
82	68.0
83	45.5
84	42.0
85	31.0
86	24.5
87	24.0
88	20.0
89	19.0
90	13.5
91	11.0
92	7.5
93	6.0
94	8.0
95	5.5
96	4.0
97	5.0
98	6.0
99	7.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05943801801644613
2	0.007208782409101616
3	0.00792047748770719
4	0.009412741362202747
5	0.0038339702621655093
6	0.002410580104954362
7	8.494425131743942E-4
8	0.0013315585341652665
9	5.280318325138126E-4
10-11	4.362002094679321E-4
12-13	3.0993172777984653E-4
14-15	1.836632460917609E-4
16-17	5.739476440367529E-4
18-19	0.0015152217802570276
20-21	8.838793718165994E-4
22-23	0.0010905005236698305
24-25	0.0012971216755230614
26-27	0.0036388280631930135
28-29	0.00392580188521139
30-31	0.005165528796330776
32-33	0.006118281885431786
34-35	0.003386291099816842
36-37	0.007771251100257633
38-39	0.005992013403743699
40-41	0.005636165864440913
42-43	0.013809180315524274
44-45	0.01972084104910283
46-47	0.018354845656295356
48-49	0.021270499688002063
50-51	0.018171182410203594
52-53	0.024978201468479483
54-55	0.018504072043744914
56-57	0.014980033509359251
58-59	0.01735617675567141
60-61	0.015909828692698788
62-63	0.010732820943487278
64-65	0.01112310534143227
66-67	0.013372980106056342
68-69	0.00499334450311975
70-71	0.008299282932771447
72-73	0.007794209006019104
74-75	0.010916484189579039
76-77	0.010308099686900081
78-79	0.012041421571891076
80-81	0.009619362514055978
82-83	0.005888702827817084
84-85	0.010422889215707432
86-87	0.0088732305768082
88-89	0.007116950786055736
90-91	0.012523537592881948
92-93	0.00792047748770719
94-95	0.0069218085870832394
96-97	0.008609214660551293
98-99	0.008551819896147618
100	0.2600671564659335
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4355798.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.38395678925852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75117471363599	52.48549015142115
2	14.222145817990889	19.451332101360457
3	4.663952496454226	9.568185779540405
4	1.9339385027365603	5.29001468016881
5	0.9029674770149188	3.087424446514699
6	0.4959909490008339	2.0350694174601807
7	0.2918542615141647	1.3970704445701918
8	0.18575083019800415	1.016190139666337
9	0.12557795982955464	0.7728766000800756
>10	0.41710153174102954	4.358623724373479
>50	0.007574087118135164	0.3427021302530408
>100	0.001971372765807638	0.19502038459117227
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.591581152294023E-5
2	0.0	0.0	0.0	0.0	4.591581152294023E-5
3	0.0	0.0	0.0	0.0	4.591581152294023E-5
4	0.0	0.0	0.0	0.0	6.887371728441034E-5
5	0.0	0.0	0.0	0.0	6.887371728441034E-5
6	0.0	0.0	0.0	0.0	9.183162304588045E-5
7	0.0	0.0	0.0	0.0	9.183162304588045E-5
8	0.0	0.0	0.0	0.0	9.183162304588045E-5
9	0.0	0.0	0.0	0.0	1.1478952880735057E-4
10-11	0.0	0.0	0.0	0.0	1.3774743456882067E-4
12-13	0.0	0.0	0.0	0.0	3.5584753930278674E-4
14-15	0.0	0.0	0.0	0.0	4.821160209908724E-4
16-17	0.0	0.0	0.0	1.1478952880735057E-5	5.739476440367529E-4
18-19	0.0	0.0	0.0	2.2957905761470114E-5	6.657792670826333E-4
20-21	0.0	0.0	0.0	6.887371728441034E-5	7.805687958899838E-4
22-23	0.0	0.0	0.0	1.836632460917609E-4	8.264846074129242E-4
24-25	0.0	0.0	0.0	3.443685864220517E-4	8.838793718165994E-4
26-27	0.0	0.0	0.0	7.002161257248385E-4	9.183162304588046E-4
28-29	0.0	0.0	0.0	0.002823822408660824	9.412741362202747E-4
30-31	0.0	0.0	0.0	0.010021125864881705	9.642320419817449E-4
32-33	0.0	0.0	0.0	0.02364664293431422	0.001033105759266155
34-35	0.0	0.0	0.0	0.04644384335545404	0.0010790215707890953
36-37	0.0	0.0	0.0	0.08511643561065045	0.0010790215707890953
38-39	0.0	0.0	0.0	0.14619594388904167	0.0011249373823120357
40-41	0.0	0.0	0.0	0.21303788651356192	0.0011478952880735057
42-43	0.0	0.0	0.0	0.28099328756751346	0.0011708531938349758
44-45	0.0	0.0	0.0	0.36453710663350325	0.001216769005357916
46-47	0.0	0.0	0.0	0.4711191841311282	0.0012282479582386512
48-49	0.0	0.0	0.0	0.5926927740909933	0.0012741637697615913
50-51	0.0	0.0	0.0	0.7210504252033726	0.0013545164399267368
52-53	0.0	0.0	0.0	0.850797029614321	0.0015496586388992328
54-55	0.0	0.0	0.0	0.9947430987387387	0.001630011309064378
56-57	0.0	0.0	0.0	1.172230668180664	0.0016529692148258483
58-59	0.0	0.0	0.0	1.3824332533326844	0.0016874060734680535
60-61	0.0	0.0	0.0	1.6013483637211827	0.0017218429321102587
62-63	0.0	0.0	0.0	1.8299976261525441	0.0018825482724405495
64-65	0.0	0.0	0.0	2.0695633727734846	0.0018825482724405495
66-67	0.0	0.0	0.0	2.3479394590841904	0.0019399430368442247
68-69	0.0	0.0	0.0	2.6682596392211027	0.0019973378012479
70-71	0.0	0.0	0.0	3.020812719047118	0.0020202957070093704
72-73	0.0	0.0	0.0	3.388977174790934	0.0021236062829359855
74-75	0.0	0.0	0.0	3.7479469892772803	0.002203958953101131
76-77	0.0	0.0	0.0	4.151099293401577	0.002226916858862601
78-79	0.0	0.0	0.0	4.595242938262977	0.002272832670385541
80-81	0.0	0.0	0.0	5.083465762186401	0.0023072695290277463
82-83	0.0	0.0	0.0	5.598090177735515	0.002433538010715832
84-85	0.0	0.0	0.0	6.093808757890058	0.002433538010715832
86-87	0.0	0.0	0.0	6.612554117523356	0.002490932775119507
88	0.0	0.0	0.0	7.039513769922297	0.0025483275395231826
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4260	0.0	34.83123	94
CAACGCA	6285	0.0	33.12516	5
GTATCAA	7130	0.0	32.840805	1
GTACTAG	3730	0.0	32.52257	1
CTGTGCG	5205	0.0	30.967634	9
TAGTACT	5250	0.0	30.615496	4
ATCAACG	6815	0.0	30.481472	3
GGTATCA	4520	0.0	30.271124	1
TAGGCAT	4560	0.0	30.196898	5
TCTAACG	1815	0.0	29.51975	2
CTAACGC	1880	0.0	28.498795	3
CTAGTAC	5980	0.0	28.057343	3
GTACTGT	5870	0.0	28.020414	6
GTACATG	16140	0.0	27.908466	1
AACGCAG	7990	0.0	27.584852	6
TACATGG	16270	0.0	27.240095	2
TAACGCC	1925	0.0	27.099842	4
CTAGGCA	5185	0.0	27.011084	4
ACATGGG	15865	0.0	26.83908	3
GCATCGT	4150	0.0	26.837364	8
>>END_MODULE