FastQCFastQC Report
Mon 16 Jan 2017
ERR1146675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4355798
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103160.2368337558353257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85040.19523403059554184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54330.12473030200206713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73100.045.8800051
GTATCAA127750.040.5762561
GGGCACC41750.032.6729194
CAACGCA152750.032.4299745
ATCAACG153900.032.2113083
GTGGTAT22300.031.0072861
CTGTGCG48300.030.5590279
TATCAAC168350.030.4554272
AACGCAG173400.029.3800436
TAGTACT50850.028.9507474
GTACTAG33800.028.9466271
TCAACGC180300.027.8078774
CTAGTAC55350.027.7866693
GTACTGT55150.027.5251226
TCTAACG17350.026.8402772
ACGCAGA188450.026.2107127
TAACGCC18100.025.9853044
CTAACGC17950.025.9404263
ACTGTGC63850.025.7625798
TAGGCAT44500.025.453315
Produced by FastQC (version 0.11.5)