##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4355798
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.129190563933406	33.0	31.0	34.0	30.0	34.0
2	32.194427519366144	33.0	31.0	34.0	30.0	34.0
3	32.34691301111759	34.0	31.0	34.0	30.0	34.0
4	35.95343356142778	37.0	35.0	37.0	35.0	37.0
5	35.858970732802575	37.0	35.0	37.0	35.0	37.0
6	35.906932782466036	37.0	35.0	37.0	35.0	37.0
7	35.82603486203906	37.0	35.0	37.0	35.0	37.0
8	35.832248189654344	37.0	35.0	37.0	35.0	37.0
9	37.54340857863473	39.0	37.0	39.0	35.0	39.0
10-11	37.49008057765764	39.0	37.0	39.0	34.5	39.0
12-13	37.462233211916626	39.0	37.0	39.0	34.0	39.0
14-15	38.701940953184696	40.0	38.0	41.0	34.0	41.0
16-17	38.69522553617041	40.0	38.0	41.0	34.0	41.0
18-19	38.75072661771735	40.0	38.0	41.0	34.0	41.0
20-21	38.7488511863957	40.0	38.0	41.0	34.0	41.0
22-23	38.71388686986862	40.0	38.0	41.0	34.0	41.0
24-25	38.652955325292865	40.0	38.0	41.0	34.0	41.0
26-27	38.51772729130231	40.0	38.0	41.0	34.0	41.0
28-29	38.45324748760159	40.0	38.0	41.0	34.0	41.0
30-31	38.32371048887023	40.0	38.0	41.0	34.0	41.0
32-33	38.18923788476876	40.0	37.0	41.0	33.5	41.0
34-35	38.09019874199859	40.0	37.0	41.0	33.0	41.0
36-37	38.07806032327486	40.0	37.0	41.0	33.0	41.0
38-39	37.9898138067927	40.0	37.0	41.0	33.0	41.0
40-41	37.883041293466775	40.0	37.0	41.0	33.0	41.0
42-43	37.746197252489665	40.0	37.0	41.0	33.0	41.0
44-45	37.56822469728853	40.0	36.5	41.0	32.0	41.0
46-47	37.340750420474045	40.0	36.0	41.0	31.5	41.0
48-49	37.10419629193089	40.0	36.0	41.0	31.0	41.0
50-51	36.909764754931246	39.0	35.0	41.0	31.0	41.0
52-53	36.74126440206823	39.0	35.0	41.0	31.0	41.0
54-55	36.562240259993686	39.0	35.0	41.0	30.5	41.0
56-57	36.3602610818959	38.5	35.0	41.0	30.0	41.0
58-59	36.1071896584736	38.0	35.0	40.0	30.0	41.0
60-61	35.92775388114876	37.5	35.0	40.0	30.0	41.0
62-63	35.71598246750653	37.0	35.0	40.0	30.0	41.0
64-65	35.466164064541104	36.5	34.5	40.0	29.5	41.0
66-67	35.19720852528056	36.0	34.0	39.0	29.0	41.0
68-69	34.92405387026671	35.5	34.0	39.0	29.0	41.0
70-71	34.665043351413445	35.0	34.0	39.0	29.0	41.0
72-73	34.39799458101592	35.0	34.0	37.5	29.0	40.0
74-75	34.123442822646965	35.0	34.0	37.0	29.0	39.5
76-77	33.86358883033603	35.0	34.0	36.5	29.0	39.0
78-79	33.62198292941959	35.0	34.0	36.0	29.0	39.0
80-81	33.47440113154926	35.0	34.0	36.0	29.0	38.0
82-83	33.31689945217845	35.0	33.0	35.5	29.0	37.0
84-85	33.1825301586529	35.0	33.0	35.0	29.0	37.0
86-87	33.03743584987183	35.0	33.0	35.0	29.0	36.5
88-89	32.93007033384009	35.0	33.0	35.0	29.0	36.0
90-91	32.86851640043914	35.0	33.0	35.0	29.0	36.0
92-93	32.80948003098399	35.0	33.0	35.0	29.0	36.0
94-95	32.7567708603567	35.0	33.0	35.0	29.0	35.0
96-97	32.6663668746806	35.0	33.0	35.0	28.5	35.0
98-99	32.589542260683345	35.0	33.0	35.0	27.0	35.0
100	31.366473376405427	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39115832949769924
1101	2	0.4235527429740955
1101	3	0.3717039180398487
1101	4	0.2949414680934197
1101	5	0.24057919558310203
1101	6	0.2768697399711968
1101	7	0.30164025082188317
1101	8	0.28535902574023453
1101	9	0.4046871213247343
1101	10-11	0.38442292747048157
1101	12-13	0.3782877813841239
1101	14-15	0.4960373760203538
1101	16-17	0.5798590843378122
1101	18-19	0.49821942655180607
1101	20-21	0.50020973791149
1101	22-23	0.5156128886693665
1101	24-25	0.4490466455313751
1101	26-27	0.49687629581559634
1101	28-29	0.48362375139057434
1101	30-31	0.5556590409345503
1101	32-33	0.6480522728572211
1101	34-35	0.627916506391692
1101	36-37	0.5850053195790252
1101	38-39	0.5948711139972431
1101	40-41	0.545431860261381
1101	42-43	0.49766147928396975
1101	44-45	0.5050365044161396
1101	46-47	0.508378532819755
1101	48-49	0.5568694994417456
1101	50-51	0.6003382323890776
1101	52-53	0.5544699274669114
1101	54-55	0.5530127111556453
1101	56-57	0.5744571794103663
1101	58-59	0.557127653478048
1101	60-61	0.5601942427202005
1101	62-63	0.5596794292693446
1101	64-65	0.6081738507688925
1101	66-67	0.5121386432252422
1101	68-69	0.49674119396677696
1101	70-71	0.5322164896004651
1101	72-73	0.5229068215690234
1101	74-75	0.49527306612106514
1101	76-77	0.4713226273634561
1101	78-79	0.4754955094641957
1101	80-81	0.5078553328330813
1101	82-83	0.5081598048448228
1101	84-85	0.4807355574167289
1101	86-87	0.42641341586927695
1101	88-89	0.4582589665215906
1101	90-91	0.45326460409513913
1101	92-93	0.4340956923117645
1101	94-95	0.47919511039879836
1101	96-97	0.4814232630376267
1101	98-99	0.4739391433079376
1101	100	0.809834092627046
1102	1	0.31332208910725967
1102	2	0.3806978806428276
1102	3	0.3080008193276669
1102	4	0.21287872381391537
1102	5	0.22403937557336207
1102	6	0.21209328093676305
1102	7	0.2917081188683781
1102	8	0.2783288455683248
1102	9	0.3141203078892758
1102	10-11	0.27787032336699724
1102	12-13	0.3469464927351993
1102	14-15	0.4705859454714556
1102	16-17	0.4718314589879711
1102	18-19	0.48622820862178884
1102	20-21	0.45450883077214144
1102	22-23	0.45658885787494796
1102	24-25	0.4977564728036654
1102	26-27	0.5137755177979884
1102	28-29	0.538944286085254
1102	30-31	0.5807999759377722
1102	32-33	0.5444777986567004
1102	34-35	0.542700898567233
1102	36-37	0.5264592982781835
1102	38-39	0.5400750846001117
1102	40-41	0.5097440113936997
1102	42-43	0.49104978569761926
1102	44-45	0.518369002716156
1102	46-47	0.4897715792486892
1102	48-49	0.5569551739566947
1102	50-51	0.5287624285960604
1102	52-53	0.567836953680029
1102	54-55	0.4788883796956682
1102	56-57	0.48869476193829087
1102	58-59	0.48033675904878237
1102	60-61	0.4488824966212377
1102	62-63	0.4811582067046061
1102	64-65	0.49790676883873175
1102	66-67	0.4644454901104069
1102	68-69	0.4705179866411697
1102	70-71	0.49084772764140183
1102	72-73	0.46801776403933815
1102	74-75	0.45651282955664385
1102	76-77	0.4345072400306407
1102	78-79	0.4427580318105484
1102	80-81	0.4233218947894315
1102	82-83	0.44194056360277045
1102	84-85	0.4274348447635248
1102	86-87	0.42221356275656774
1102	88-89	0.4009573380264513
1102	90-91	0.388630749237727
1102	92-93	0.34648558101938676
1102	94-95	0.3691111871162249
1102	96-97	0.36954167244916647
1102	98-99	0.3857706805972825
1102	100	0.5946739622469188
1103	1	0.286712468240367
1103	2	0.3220003777046472
1103	3	0.26208994279684106
1103	4	0.24405611749919132
1103	5	0.17932055286008364
1103	6	0.224432055253871
1103	7	0.22500527764356093
1103	8	0.24008250999528968
1103	9	0.23585614665263677
1103	10-11	0.2633296531823248
1103	12-13	0.24210674758067796
1103	14-15	0.3621642868100423
1103	16-17	0.3872447562066412
1103	18-19	0.34816160335157065
1103	20-21	0.3694732419569391
1103	22-23	0.33293922985459545
1103	24-25	0.3265306059393822
1103	26-27	0.3793681222344887
1103	28-29	0.3972144304090435
1103	30-31	0.3681087979774489
1103	32-33	0.39859242844569565
1103	34-35	0.3985963595927089
1103	36-37	0.32815164295476507
1103	38-39	0.32923868267261724
1103	40-41	0.32014096113163504
1103	42-43	0.32425790885319117
1103	44-45	0.38683734904287803
1103	46-47	0.42713935033670936
1103	48-49	0.4015899905324858
1103	50-51	0.3951667857438679
1103	52-53	0.3786936280333748
1103	54-55	0.3393523266041143
1103	56-57	0.32644875494541026
1103	58-59	0.41738892775623526
1103	60-61	0.38837134959174335
1103	62-63	0.35965164672444416
1103	64-65	0.33991609353662966
1103	66-67	0.3645256528298404
1103	68-69	0.31521995013103066
1103	70-71	0.31197986454428417
1103	72-73	0.28789047440682225
1103	74-75	0.2611309970253757
1103	76-77	0.30415144172466313
1103	78-79	0.28605826484480446
1103	80-81	0.24303535406866672
1103	82-83	0.22246367300356695
1103	84-85	0.25771429286866265
1103	86-87	0.2442787446548209
1103	88-89	0.30047902857135256
1103	90-91	0.30934603809804884
1103	92-93	0.34014770858635046
1103	94-95	0.32655836935361293
1103	96-97	0.3628271474236229
1103	98-99	0.30808498111206717
1103	100	0.6211859408047822
1104	1	0.2381521193944991
1104	2	0.2594650565197796
1104	3	0.1912623947114369
1104	4	0.168799439526083
1104	5	0.15384396211860718
1104	6	0.16125625394661114
1104	7	0.2115444909892048
1104	8	0.15656470885158313
1104	9	0.18271385394145767
1104	10-11	0.2410870595832506
1104	12-13	0.30147186062863796
1104	14-15	0.3486389662192977
1104	16-17	0.3124807067641342
1104	18-19	0.29550282809314155
1104	20-21	0.35147003861656145
1104	22-23	0.3409311120790477
1104	24-25	0.33060557275901203
1104	26-27	0.39163064743378584
1104	28-29	0.4664049985845722
1104	30-31	0.37417248961474314
1104	32-33	0.35322469296593795
1104	34-35	0.41265307950200025
1104	36-37	0.3846582613334135
1104	38-39	0.42922962202084847
1104	40-41	0.4685950530347469
1104	42-43	0.47317237950942825
1104	44-45	0.47507457391353114
1104	46-47	0.4922318817558349
1104	48-49	0.44105569583947357
1104	50-51	0.43925118866043533
1104	52-53	0.3729930386348741
1104	54-55	0.4236126748632003
1104	56-57	0.39944610335815867
1104	58-59	0.3963195773946637
1104	60-61	0.4067891585468999
1104	62-63	0.4111735199125377
1104	64-65	0.3185658927674382
1104	66-67	0.33410198748012476
1104	68-69	0.42383741844418665
1104	70-71	0.3893891292108549
1104	72-73	0.3263313152499734
1104	74-75	0.3857847989876291
1104	76-77	0.3163569281384042
1104	78-79	0.3079415610828704
1104	80-81	0.27908343103758426
1104	82-83	0.23640579962035702
1104	84-85	0.23850807197597135
1104	86-87	0.2549320242944546
1104	88-89	0.24927427819517334
1104	90-91	0.26713741701509974
1104	92-93	0.2950509904263967
1104	94-95	0.27634442290868577
1104	96-97	0.26327854804039674
1104	98-99	0.25990086820970504
1104	100	0.4619731443497912
1105	1	0.14644955216451194
1105	2	0.17465602251398593
1105	3	0.09354261043006318
1105	4	0.12451782039708803
1105	5	0.10172129035574784
1105	6	0.08632476667948907
1105	7	0.1380244312437071
1105	8	0.09274541070895737
1105	9	0.16784311227549864
1105	10-11	0.16349109012128338
1105	12-13	0.13594451369385752
1105	14-15	0.2059867462002103
1105	16-17	0.22113395189241913
1105	18-19	0.2018156744615638
1105	20-21	0.2396197409761598
1105	22-23	0.19051542921283016
1105	24-25	0.19665794844686246
1105	26-27	0.23125921656397708
1105	28-29	0.2917246386114485
1105	30-31	0.2681894609877773
1105	32-33	0.2223695855740644
1105	34-35	0.19201931905794822
1105	36-37	0.2304390483085399
1105	38-39	0.24932791781757402
1105	40-41	0.2961557605883769
1105	42-43	0.30801436539487526
1105	44-45	0.23313575869191538
1105	46-47	0.2946520716238794
1105	48-49	0.3223221242198804
1105	50-51	0.2777038852520306
1105	52-53	0.25197586778406844
1105	54-55	0.29514572947119433
1105	56-57	0.2173949854129873
1105	58-59	0.21301988894958868
1105	60-61	0.2605072719981223
1105	62-63	0.29188540552644326
1105	64-65	0.27324734253056704
1105	66-67	0.22642774609353467
1105	68-69	0.2230351183078696
1105	70-71	0.2674612635251563
1105	72-73	0.19794576439781508
1105	74-75	0.3054547747024401
1105	76-77	0.22056119391888984
1105	78-79	0.21983584438695658
1105	80-81	0.10902115809981439
1105	82-83	0.16967613711283036
1105	84-85	0.18771964560011867
1105	86-87	0.2160494177776684
1105	88-89	0.21370167100440796
1105	90-91	0.20931393418758404
1105	92-93	0.17861861502119325
1105	94-95	0.19453387406761635
1105	96-97	0.20208602427155853
1105	98-99	0.19959722988735962
1105	100	0.3383867460160559
1106	1	0.15316276759236302
1106	2	0.22277476696038434
1106	3	0.1500403574158966
1106	4	0.1279635112537747
1106	5	0.12689686308142
1106	6	0.11452077665679639
1106	7	0.13105682142336406
1106	8	0.09121913456474573
1106	9	0.11875478239272752
1106	10-11	0.16262062377666808
1106	12-13	0.13392868621295406
1106	14-15	0.2483737008196698
1106	16-17	0.19845063231623072
1106	18-19	0.22519769906203635
1106	20-21	0.19930670957830188
1106	22-23	0.26065645121072123
1106	24-25	0.2639997976775845
1106	26-27	0.2502499609382127
1106	28-29	0.19141873386500663
1106	30-31	0.24398475920205698
1106	32-33	0.25512069854952557
1106	34-35	0.2431846393287671
1106	36-37	0.15985847429736566
1106	38-39	0.20338801172699306
1106	40-41	0.15534654736167397
1106	42-43	0.2211614360254046
1106	44-45	0.15074961596944547
1106	46-47	0.07886629064662998
1106	48-49	0.16043326840828342
1106	50-51	0.20058717956920447
1106	52-53	0.1921084776269737
1106	54-55	0.15720979716536476
1106	56-57	0.1656976245292796
1106	58-59	0.05617354496686744
1106	60-61	0.09107223954479338
1106	62-63	0.1513353904161363
1106	64-65	0.12209742575000604
1106	66-67	0.0857063756114087
1106	68-69	0.10575413879924866
1106	70-71	0.14552987333197365
1106	72-73	0.11705369257200005
1106	74-75	0.0353051826806734
1106	76-77	0.12468565321804448
1106	78-79	0.12422498210724342
1106	80-81	0.06967552452697845
1106	82-83	0.11965144191249522
1106	84-85	0.1101313405253066
1106	86-87	0.0442026227410679
1106	88-89	0.06610608140932328
1106	90-91	0.09395588761176299
1106	92-93	0.07738759875167744
1106	94-95	0.09353298549145705
1106	96-97	-0.003990054987632163
1106	98-99	-0.011935766959773275
1106	100	0.22922868320229384
1107	1	0.11780464460517237
1107	2	0.13446943936707356
1107	3	0.14197880430418763
1107	4	0.13752915225567364
1107	5	-0.004910868310354033
1107	6	0.04036968894074988
1107	7	0.08771032836803982
1107	8	0.07543236173857792
1107	9	0.07141961507468864
1107	10-11	0.14445759532124924
1107	12-13	0.030292315562874705
1107	14-15	0.1947792781611426
1107	16-17	0.176936007831209
1107	18-19	0.15609056525830312
1107	20-21	0.1051124835440902
1107	22-23	0.07331827501153043
1107	24-25	0.06622116756717134
1107	26-27	0.17865667570288934
1107	28-29	0.1277396326938529
1107	30-31	0.1063600669786382
1107	32-33	0.2159355212018852
1107	34-35	0.19207257669113176
1107	36-37	0.1444389502957506
1107	38-39	0.1484853760352678
1107	40-41	0.2163732150864135
1107	42-43	0.23385945457246038
1107	44-45	0.2267031337152332
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2216	68-69	-0.8786109155342601
2216	70-71	-0.9145249166690874
2216	72-73	-0.8288090865395361
2216	74-75	-0.7895627444707216
2216	76-77	-0.8746219966322428
2216	78-79	-0.7995585989448202
2216	80-81	-0.8322285551026809
2216	82-83	-0.8044173044433052
2216	84-85	-0.8593780498230998
2216	86-87	-0.8087490446138048
2216	88-89	-0.744366486240402
2216	90-91	-0.7753779485598145
2216	92-93	-0.7793596534483243
2216	94-95	-0.8052447441005341
2216	96-97	-0.8171469280669719
2216	98-99	-0.8230897495838398
2216	100	-0.3297941022171962
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	11.0
13	43.0
14	199.0
15	628.0
16	1498.0
17	2996.0
18	5534.0
19	8351.0
20	12541.0
21	17764.0
22	24682.0
23	29787.0
24	31219.0
25	31693.0
26	34377.0
27	41085.0
28	52051.0
29	65469.0
30	81414.0
31	102024.0
32	128561.0
33	165774.0
34	230650.0
35	336937.0
36	551371.0
37	1037622.0
38	1024275.0
39	336415.0
40	822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.681638588382654	20.36862131806847	13.027647287592309	25.922092805956567
2	14.62794278296383	20.456063915714573	38.72660823614173	26.189385065179867
3	17.951842578558512	25.279156655106593	29.275898469120925	27.493102297213966
4	12.083729803023976	16.575186623194977	37.33764465638515	34.0034389173959
5	12.989688449424087	38.09442141279699	34.855603164752694	14.060286973026226
6	31.771299666008236	37.57733374106593	16.760013554353787	13.891353038572055
7	27.54641687847253	32.02300290853775	22.31421712260695	18.11636309038277
8	26.965621454438427	34.583169375623015	19.163491970931616	19.28771719900693
9	26.209433954467126	14.594432524189596	20.454093601218425	38.74203992012485
10-11	25.83693045453439	25.64592067859896	27.158364552258853	21.358784314607792
12-13	26.067260054456376	23.2042825854382	27.795618695159074	22.93283866494635
14-15	23.13138751910695	24.478686601227093	25.461060851227234	26.92886502843872
16-17	22.240907406633642	27.243871272267445	26.55574248392602	23.95947883717289
18-19	21.80092017634896	26.84888443454244	29.047105172866814	22.303090216241785
20-21	22.940882474347983	26.09115482398403	28.698966297335183	22.2689964043328
22-23	23.00872308587313	25.80771651945292	28.503640435116594	22.679919959557353
24-25	22.674478935891884	25.83130576762283	29.081387612556874	22.41282768392841
26-27	22.697781210239775	26.093829420005243	28.278021616245745	22.93036775350923
28-29	22.163941027568313	26.40287726841327	28.680944341312433	22.752237362705984
30-31	22.76980016061351	26.15738838210587	28.597549748633888	22.475261708646727
32-33	23.002134166919586	26.254879128921953	28.035138452242276	22.707848251916182
34-35	22.99547637424876	26.182814262736702	28.58087083009818	22.240838532916356
36-37	22.81057340124588	25.77468009306217	28.320493741904468	23.094252763787484
38-39	22.87167586743003	25.92077272637528	28.763650196818126	22.44390120937656
40-41	22.60161054300498	25.42588063082815	29.0962069407259	22.87630188544097
42-43	22.76080066155501	25.560241774297154	28.429291257308076	23.249666306839757
44-45	22.866085617377113	26.162611305666605	28.235285474670775	22.736017602285504
46-47	23.014749536135515	26.149399030900884	27.595850404449422	23.24000102851418
48-49	23.6179914679239	26.173791805772446	27.346596421597148	22.86162030470651
50-51	23.121882603371414	26.18742880179476	27.343565978036633	23.347122616797197
52-53	22.83478251287135	26.45811398967537	27.186350239382083	23.520753258071196
54-55	22.7396564303487	26.466608414807112	27.11901470178369	23.674720453060498
56-57	23.446404080262674	26.182159962422503	26.8577307763124	23.513705181002425
58-59	22.647583749292323	26.43279141962047	27.494445334701012	23.425179496386196
60-61	22.676040073483662	26.82162946950249	27.05289593319066	23.44943452382319
62-63	23.04839434702895	26.292484178559246	26.79826980039019	23.860851674021614
64-65	22.43063036009951	26.487535228922564	27.073960764002724	24.007873646975202
66-67	22.83849248047025	26.233076839545333	27.05277870311041	23.875651976874003
68-69	22.67234385065607	26.0769324013648	27.28265865405145	23.968065093927677
70-71	22.4947222420235	25.915212191067067	27.297812021935453	24.29225354497398
72-73	22.966768887105818	25.837653739597876	27.56094915207543	23.634628221220872
74-75	23.032924971910898	26.165427930047926	27.112261357432487	23.68938574060869
76-77	22.878322858969323	26.132253514772913	26.79933059269224	24.190093033565528
78-79	22.71424202867075	26.254500323476893	26.85256524751607	24.17869240033629
80-81	22.43677277963762	26.64429112644801	27.159489489641164	23.7594466042732
82-83	23.02440333550821	25.877072352758322	27.883352258300313	23.215172053433147
84-85	23.117520601276734	26.240840369548817	26.902716792651997	23.73892223652245
86-87	22.16732731866813	26.720752431586586	27.17896927267977	23.932950977065513
88-89	22.8136612395708	25.93465078040809	27.05074936900196	24.20093861101915
90-91	22.525723185510437	25.953545136849783	27.116581163772974	24.404150513866803
92-93	22.8046617405123	26.136221192993798	26.94393771244672	24.115179354047182
94-95	21.96341519969475	26.133179270480404	27.38646282495194	24.516942704872907
96-97	22.27570011281515	26.008575236960024	27.04765005172416	24.668074598500663
98-99	21.762900850774074	26.485433897531518	26.987993933602983	24.763671318091426
100	22.34086005498706	26.00316469654199	26.37198875999765	25.283986488473296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	408.0
1	303.5
2	310.0
3	414.0
4	283.0
5	217.0
6	502.0
7	1128.5
8	1835.0
9	2061.0
10	1589.0
11	1094.5
12	1028.5
13	1117.0
14	1260.0
15	1590.0
16	1988.5
17	2456.5
18	3118.5
19	3851.5
20	4775.0
21	5966.0
22	7294.5
23	8892.0
24	10698.5
25	12581.5
26	15334.0
27	18503.0
28	22479.5
29	29140.0
30	38279.5
31	47263.5
32	59676.0
33	70710.5
34	77220.5
35	86313.0
36	96226.5
37	111437.0
38	119664.5
39	117177.0
40	113602.5
41	110064.0
42	112120.5
43	120121.0
44	131546.5
45	151337.0
46	170199.0
47	183819.0
48	194475.0
49	193592.0
50	186592.5
51	190030.5
52	192296.0
53	186019.5
54	180266.5
55	160288.5
56	142513.5
57	123920.0
58	103114.0
59	97425.0
60	82855.5
61	61660.5
62	47989.5
63	38165.5
64	27936.0
65	17594.5
66	12255.5
67	9287.0
68	7116.5
69	5360.5
70	4009.5
71	3066.5
72	2413.0
73	2016.5
74	1594.0
75	1244.5
76	855.5
77	487.0
78	281.5
79	145.5
80	82.0
81	31.5
82	15.0
83	4.5
84	1.5
85	1.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007162866597578675
3	0.0
4	0.08831906346437553
5	0.0045227074350096125
6	4.591581152294023E-5
7	2.2957905761470114E-5
8	0.0
9	0.0
10-11	0.0
12-13	4.13242303706462E-4
14-15	0.008735483142239379
16-17	0.0
18-19	5.739476440367528E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	4.591581152294023E-5
66-67	1.607053403302908E-4
68-69	0.0
70-71	4.247212565871971E-4
72-73	3.558475393027868E-4
74-75	3.673264921835218E-4
76-77	1.033105759266155E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06368523058231809
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4355798.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.24513485628255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66250844552019	48.713654322363
2	15.420757509775749	20.12258806622701
3	5.1227860648579	10.02710602924616
4	2.0830747518640904	5.436419724043596
5	1.0093836560162137	3.292868637925269
6	0.5610483112396237	2.1963403636631305
7	0.3351580124007408	1.5307200802074965
8	0.200751104984549	1.0478426333811708
9	0.13582237631279168	0.7975574333125963
>10	0.4522677880936151	4.559358641289443
>50	0.010904612746440175	0.47501489885804665
>100	0.0046143358063314545	0.5583138732149268
>500	6.315471040012653E-4	0.30244745930386097
>1k	1.4574163890283755E-4	0.17103400614725098
>5k	9.716109260189169E-5	0.4417533253658705
>10k+	4.8580546300945845E-5	0.32698050545126783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10316	0.2368337558353257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8504	0.19523403059554184	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5433	0.12473030200206713	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.183162304588045E-5
2	0.0	0.0	0.0	0.0	9.183162304588045E-5
3	0.0	0.0	0.0	0.0	9.183162304588045E-5
4	0.0	0.0	0.0	0.0	9.183162304588045E-5
5	0.0	0.0	0.0	0.0	1.1478952880735057E-4
6	0.0	0.0	0.0	0.0	1.3774743456882067E-4
7	0.0	0.0	0.0	0.0	1.3774743456882067E-4
8	0.0	0.0	0.0	0.0	1.3774743456882067E-4
9	0.0	0.0	0.0	0.0	1.3774743456882067E-4
10-11	0.0	0.0	0.0	0.0	1.7218429321102586E-4
12-13	0.0	0.0	0.0	0.0	1.9514219897249596E-4
14-15	0.0	0.0	0.0	0.0	2.8697382201837643E-4
16-17	0.0	0.0	0.0	1.1478952880735057E-5	3.443685864220517E-4
18-19	0.0	0.0	0.0	2.2957905761470114E-5	3.673264921835218E-4
20-21	0.0	0.0	0.0	6.887371728441034E-5	4.591581152294023E-4
22-23	0.0	0.0	0.0	1.607053403302908E-4	4.821160209908724E-4
24-25	0.0	0.0	0.0	3.214106806605816E-4	4.821160209908724E-4
26-27	0.0	0.0	0.0	6.198634555596931E-4	5.739476440367529E-4
28-29	0.0	0.0	0.0	0.002812343455780089	6.313424084404282E-4
30-31	0.0	0.0	0.0	0.01003260481776244	6.428213613211632E-4
32-33	0.0	0.0	0.0	0.023715516651598628	6.428213613211632E-4
34-35	0.0	0.0	0.0	0.046719338224591685	7.346529843670436E-4
36-37	0.0	0.0	0.0	0.08549524105571471	7.346529843670436E-4
38-39	0.0	0.0	0.0	0.1464943966639408	7.576108901285138E-4
40-41	0.0	0.0	0.0	0.21409395017858956	7.576108901285138E-4
42-43	0.0	0.0	0.0	0.2824396356304861	7.576108901285138E-4
44-45	0.0	0.0	0.0	0.3667640234923658	7.690898430092488E-4
46-47	0.0	0.0	0.0	0.4740577960685964	7.805687958899838E-4
48-49	0.0	0.0	0.0	0.5960905441436908	7.805687958899838E-4
50-51	0.0	0.0	0.0	0.7248155217482537	8.264846074129242E-4
52-53	0.0	0.0	0.0	0.8557100214472756	9.412741362202746E-4
54-55	0.0	0.0	0.0	1.0005284909906291	9.87189947743215E-4
56-57	0.0	0.0	0.0	1.1797723402233071	0.001033105759266155
58-59	0.0	0.0	0.0	1.392029657940979	0.0010445847121468902
60-61	0.0	0.0	0.0	1.613940775031349	0.0010675426179083602
62-63	0.0	0.0	0.0	1.8452761124368027	0.0011478952880735057
64-65	0.0	0.0	0.0	2.0869195495291564	0.0011478952880735057
66-67	0.0	0.0	0.0	2.367729173850578	0.0011708531938349758
68-69	0.0	0.0	0.0	2.690689513150059	0.001193811099596446
70-71	0.0	0.0	0.0	3.046341910253873	0.001216769005357916
72-73	0.0	0.0	0.0	3.4179959676734324	0.0013200795812845314
74-75	0.0	0.0	0.0	3.78042094697688	0.0013774743456882069
76-77	0.0	0.0	0.0	4.187189121258607	0.001411911204330412
78-79	0.0	0.0	0.0	4.636383505387531	0.0014233901572111472
80-81	0.0	0.0	0.0	5.130070310882185	0.0014922638744955573
82-83	0.0	0.0	0.0	5.6500324395208406	0.001630011309064378
84-85	0.0	0.0	0.0	6.152259585958761	0.0016529692148258483
86-87	0.0	0.0	0.0	6.677593864545601	0.0016874060734680535
88	0.0	0.0	0.0	7.110614404065569	0.0017218429321102585
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7310	0.0	45.880005	1
GTATCAA	12775	0.0	40.576256	1
GGGCACC	4175	0.0	32.67291	94
CAACGCA	15275	0.0	32.429974	5
ATCAACG	15390	0.0	32.211308	3
GTGGTAT	2230	0.0	31.007286	1
CTGTGCG	4830	0.0	30.559027	9
TATCAAC	16835	0.0	30.455427	2
AACGCAG	17340	0.0	29.380043	6
TAGTACT	5085	0.0	28.950747	4
GTACTAG	3380	0.0	28.946627	1
TCAACGC	18030	0.0	27.807877	4
CTAGTAC	5535	0.0	27.786669	3
GTACTGT	5515	0.0	27.525122	6
TCTAACG	1735	0.0	26.840277	2
ACGCAGA	18845	0.0	26.210712	7
TAACGCC	1810	0.0	25.985304	4
CTAACGC	1795	0.0	25.940426	3
ACTGTGC	6385	0.0	25.762579	8
TAGGCAT	4450	0.0	25.45331	5
>>END_MODULE