FastQCFastQC Report
Mon 16 Jan 2017
ERR1146674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155675
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC30250.035.50747394
CAACGCA50450.034.093855
GTACTAG29350.033.644581
GTATCAA59000.032.517121
ATCAACG54300.031.678523
TAGGCAT38950.029.8020135
CTGTGCG39500.029.624539
GGTATCA37950.028.8700851
TAGTACT40050.028.6332724
GCATCGT32050.028.5927438
CTAACGC14150.027.9001483
CTAGGCA42200.027.731324
ATACGGG19400.027.6176533
AACGCAG67350.027.4920986
GTACATG122050.026.7377991
TATCAAC70950.026.6958162
TACGGGC19900.026.687574
TCTAACG14700.026.5369682
GGCGTCG19700.026.4792678
TACATGG122850.026.0915412
Produced by FastQC (version 0.11.5)