##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146674_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3155675
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.397892051621284	31.0	31.0	34.0	28.0	34.0
2	31.491457136745705	33.0	31.0	34.0	28.0	34.0
3	31.57216855347905	33.0	31.0	34.0	28.0	34.0
4	34.97371117114405	37.0	35.0	37.0	32.0	37.0
5	35.142612277880325	37.0	35.0	37.0	32.0	37.0
6	35.17703027086122	37.0	35.0	37.0	32.0	37.0
7	35.139176245969566	37.0	35.0	37.0	32.0	37.0
8	35.11373509629477	37.0	35.0	37.0	32.0	37.0
9	36.60809620762594	39.0	37.0	39.0	32.0	39.0
10-11	36.4532599206192	39.0	37.0	39.0	31.5	39.0
12-13	36.401621523128966	39.0	37.0	39.0	32.0	39.0
14-15	37.59008278735928	40.0	37.5	41.0	31.0	41.0
16-17	37.61420868752327	40.0	37.5	41.0	31.0	41.0
18-19	37.57550001188335	40.0	37.0	41.0	31.0	41.0
20-21	37.64884818620422	40.0	37.0	41.0	31.0	41.0
22-23	37.59102901914804	40.0	37.0	41.0	31.0	41.0
24-25	37.55758672867136	40.0	37.0	41.0	31.0	41.0
26-27	37.60866597479145	40.0	37.0	41.0	31.0	41.0
28-29	37.59231178749396	40.0	37.0	41.0	31.0	41.0
30-31	37.45369041488746	40.0	37.0	41.0	31.0	41.0
32-33	37.45001402235655	40.0	37.0	41.0	31.0	41.0
34-35	37.38732268056755	40.0	37.0	41.0	30.0	41.0
36-37	37.26795772695223	40.0	36.0	41.0	30.0	41.0
38-39	37.16792334445087	40.0	36.0	41.0	30.0	41.0
40-41	37.086585120457585	40.0	36.0	41.0	30.0	41.0
42-43	36.91514794774494	40.0	35.5	41.0	30.0	41.0
44-45	36.7633994311835	39.0	35.0	41.0	30.0	41.0
46-47	36.76349671623345	39.0	35.0	41.0	30.0	41.0
48-49	36.63648537951468	39.0	35.0	41.0	29.5	41.0
50-51	36.46885404865837	39.0	35.0	41.0	29.0	41.0
52-53	36.26254240376464	39.0	35.0	41.0	29.0	41.0
54-55	36.049164758728324	38.0	35.0	40.0	28.5	41.0
56-57	35.82187012287387	38.0	34.0	40.0	28.0	41.0
58-59	35.538216546380724	37.5	34.0	40.0	28.0	41.0
60-61	35.284680456637645	37.0	34.0	40.0	28.0	41.0
62-63	34.98286388807466	36.5	34.0	40.0	27.0	41.0
64-65	34.8297527470351	36.0	33.5	39.5	27.0	41.0
66-67	34.56719988591981	35.5	33.0	39.0	27.0	41.0
68-69	34.43778066499243	35.0	33.0	39.0	27.0	41.0
70-71	34.323287410775826	35.0	33.0	39.0	27.0	41.0
72-73	34.08524863935608	35.0	33.0	37.5	27.0	40.0
74-75	33.82736514374896	35.0	33.0	37.0	27.0	40.0
76-77	33.550859324867105	35.0	33.0	37.0	26.0	39.0
78-79	33.288646961426636	35.0	33.0	36.0	26.0	39.0
80-81	33.07937255896124	35.0	33.0	36.0	26.0	38.5
82-83	32.827182932336186	35.0	33.0	35.5	26.0	37.0
84-85	32.63855672716613	35.0	33.0	35.0	26.0	37.0
86-87	32.460545208235956	35.0	32.5	35.0	25.0	36.5
88-89	32.319174661522496	35.0	32.0	35.0	25.0	36.0
90-91	32.17366886640735	35.0	32.0	35.0	25.0	36.0
92-93	32.02282586134504	35.0	32.0	35.0	25.0	36.0
94-95	31.936717501010087	35.0	32.0	35.0	25.0	35.5
96-97	31.82735532017714	35.0	32.0	35.0	24.5	35.0
98-99	31.729828325160227	35.0	32.0	35.0	24.0	35.0
100	30.776465257036925	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33023770142432696
1101	2	0.4209144440889716
1101	3	0.4382410890372377
1101	4	0.4963232543819629
1101	5	0.41257816349944676
1101	6	0.43210070282262336
1101	7	0.45580616159617904
1101	8	0.4808693376817601
1101	9	0.6134632053485447
1101	10-11	0.5952439326889021
1101	12-13	0.5029012253112981
1101	14-15	0.7280279187388459
1101	16-17	0.7754246742222932
1101	18-19	0.7503128686003393
1101	20-21	0.7053889475619854
1101	22-23	0.7494798627388732
1101	24-25	0.7533191983301393
1101	26-27	0.6662343341844661
1101	28-29	0.7172517348650587
1101	30-31	0.6957112003654089
1101	32-33	0.6822841031841094
1101	34-35	0.7301647269117382
1101	36-37	0.6842310429449654
1101	38-39	0.7008910784907343
1101	40-41	0.6625969210487241
1101	42-43	0.6809432571790026
1101	44-45	0.6732621650833508
1101	46-47	0.6347975304191849
1101	48-49	0.6330211762864693
1101	50-51	0.6087925439559996
1101	52-53	0.560708947515991
1101	54-55	0.6039519739829871
1101	56-57	0.5775904481925309
1101	58-59	0.5693734297786719
1101	60-61	0.5861994302494793
1101	62-63	0.5357165200975302
1101	64-65	0.5166752235269954
1101	66-67	0.538165424927513
1101	68-69	0.6028949814010076
1101	70-71	0.5834606503317943
1101	72-73	0.5430354039567362
1101	74-75	0.4962032701707315
1101	76-77	0.5673228999982811
1101	78-79	0.5862144618482716
1101	80-81	0.5501799448494822
1101	82-83	0.5467643565633722
1101	84-85	0.46782455464334305
1101	86-87	0.5031210145335905
1101	88-89	0.586040459434237
1101	90-91	0.5734668704225854
1101	92-93	0.5717294738023497
1101	94-95	0.5799476593529214
1101	96-97	0.5680130255753539
1101	98-99	0.5220841467004895
1101	100	0.6191782615540937
1102	1	0.4172898911898777
1102	2	0.5117518468185231
1102	3	0.48757315562244585
1102	4	0.5253477170096801
1102	5	0.3796671223635357
1102	6	0.44287917478521877
1102	7	0.45124663920903885
1102	8	0.4271854902619623
1102	9	0.5487478575259814
1102	10-11	0.6772429729923104
1102	12-13	0.5038694975105429
1102	14-15	0.7487112111533847
1102	16-17	0.6981659184361675
1102	18-19	0.6959325666620586
1102	20-21	0.6396436602889537
1102	22-23	0.6685894839863167
1102	24-25	0.695219686657687
1102	26-27	0.6906563964144325
1102	28-29	0.6540108124771535
1102	30-31	0.7927638911773727
1102	32-33	0.7005396330021583
1102	34-35	0.6969931416394246
1102	36-37	0.7056172456683427
1102	38-39	0.740868704841148
1102	40-41	0.6123281867305153
1102	42-43	0.7196561267489088
1102	44-45	0.7102176143485508
1102	46-47	0.6842010924737849
1102	48-49	0.605772826848991
1102	50-51	0.6033742087405258
1102	52-53	0.5424547666690671
1102	54-55	0.5215252134793218
1102	56-57	0.5871815365038842
1102	58-59	0.4814156949709769
1102	60-61	0.45856016825231194
1102	62-63	0.5244035665059883
1102	64-65	0.5338509983181936
1102	66-67	0.5950392036268752
1102	68-69	0.4775404910615748
1102	70-71	0.5182300918962923
1102	72-73	0.4802849289261957
1102	74-75	0.4113852656458903
1102	76-77	0.47881815740123557
1102	78-79	0.45337616879778153
1102	80-81	0.512479189040242
1102	82-83	0.48068279006102443
1102	84-85	0.5404847642349111
1102	86-87	0.4773923774183899
1102	88-89	0.46679671191538574
1102	90-91	0.4671338364323603
1102	92-93	0.5353886735926778
1102	94-95	0.5610246778954853
1102	96-97	0.4956717512987012
1102	98-99	0.4249954190885994
1102	100	0.5168179475136192
1103	1	0.39160840097281735
1103	2	0.3656390255714719
1103	3	0.4109988990920108
1103	4	0.49050345659846784
1103	5	0.39577044890237545
1103	6	0.3556968424779683
1103	7	0.29153114135853997
1103	8	0.3838986634160477
1103	9	0.4140041958088645
1103	10-11	0.6239584444686201
1103	12-13	0.5836874967645258
1103	14-15	0.7271285525597335
1103	16-17	0.6392265753041926
1103	18-19	0.6592212962173605
1103	20-21	0.529555815399327
1103	22-23	0.6664803965709538
1103	24-25	0.7443047848638642
1103	26-27	0.580585412167359
1103	28-29	0.6216102149133746
1103	30-31	0.6189754084091419
1103	32-33	0.6326879802824052
1103	34-35	0.6713124049706352
1103	36-37	0.6478251797780743
1103	38-39	0.6380412033434695
1103	40-41	0.6206246589942879
1103	42-43	0.5777819203294356
1103	44-45	0.5572975206071433
1103	46-47	0.5851314420071532
1103	48-49	0.6065304254413633
1103	50-51	0.5218962368403481
1103	52-53	0.5095026659648738
1103	54-55	0.6138135888669325
1103	56-57	0.5441763483240507
1103	58-59	0.6504417526701687
1103	60-61	0.6150446395063298
1103	62-63	0.5443779647035001
1103	64-65	0.5393292942441335
1103	66-67	0.5456167041826134
1103	68-69	0.5334167546678259
1103	70-71	0.49181097211602065
1103	72-73	0.44168498393521816
1103	74-75	0.4651217347447627
1103	76-77	0.4047519920953562
1103	78-79	0.4625557054409484
1103	80-81	0.4629566560630778
1103	82-83	0.5325640233181232
1103	84-85	0.4660338183434476
1103	86-87	0.4162148075362069
1103	88-89	0.4640504994373771
1103	90-91	0.48373186741073226
1103	92-93	0.46631420041710925
1103	94-95	0.4304770555286659
1103	96-97	0.46022615936769284
1103	98-99	0.44836172643833194
1103	100	0.5303612821907784
1104	1	0.2656226970858562
1104	2	0.2677130575686455
1104	3	0.32870482705142834
1104	4	0.4469682813461233
1104	5	0.36864352792257193
1104	6	0.34545483744182803
1104	7	0.33580913223164544
1104	8	0.3627832213279589
1104	9	0.36336348486427283
1104	10-11	0.5321064605187971
1104	12-13	0.42208070987526725
1104	14-15	0.4907494366757561
1104	16-17	0.5071999515235603
1104	18-19	0.4370319229424595
1104	20-21	0.48834770980839437
1104	22-23	0.521063403516294
1104	24-25	0.48143936323862846
1104	26-27	0.445201876122546
1104	28-29	0.40603118970403784
1104	30-31	0.3946546387575438
1104	32-33	0.41514898645007037
1104	34-35	0.5172311665818299
1104	36-37	0.43854345272333006
1104	38-39	0.5014276623420031
1104	40-41	0.44987555229626963
1104	42-43	0.5034122403321604
1104	44-45	0.4554186879219131
1104	46-47	0.44165862607830775
1104	48-49	0.4746262826093002
1104	50-51	0.3613652998161996
1104	52-53	0.40511289407886153
1104	54-55	0.4444406211391936
1104	56-57	0.4449132529572566
1104	58-59	0.48423041130347855
1104	60-61	0.3776981633937382
1104	62-63	0.4720149442061867
1104	64-65	0.4411425044855193
1104	66-67	0.35622859484730185
1104	68-69	0.34145563392635836
1104	70-71	0.4088733225069703
1104	72-73	0.3886326629907444
1104	74-75	0.25864578986633546
1104	76-77	0.29857071701012217
1104	78-79	0.33860118003081396
1104	80-81	0.3858371135634897
1104	82-83	0.441753138824744
1104	84-85	0.3276717962880298
1104	86-87	0.31655079499561367
1104	88-89	0.3431232245457423
1104	90-91	0.3581125125861391
1104	92-93	0.2673369379854904
1104	94-95	0.39828821911909884
1104	96-97	0.40126303317444467
1104	98-99	0.3954513694584314
1104	100	0.4235797251281923
1105	1	0.022477702397026178
1105	2	0.20504590527419353
1105	3	0.20849470703936035
1105	4	0.23886742599824373
1105	5	0.07522803123652011
1105	6	0.1108935740545931
1105	7	0.1041372483296712
1105	8	0.18974251357440863
1105	9	0.28816733855801147
1105	10-11	0.32350787197046316
1105	12-13	0.16450233437077344
1105	14-15	0.27945870531775086
1105	16-17	0.23057329799335946
1105	18-19	0.23097103945357134
1105	20-21	0.26580659406489104
1105	22-23	0.314265897822672
1105	24-25	0.28010856582431387
1105	26-27	0.255957420259179
1105	28-29	0.2999256418652223
1105	30-31	0.37929258079646644
1105	32-33	0.3177097910222457
1105	34-35	0.3573876540940901
1105	36-37	0.33451604518234745
1105	38-39	0.3164626359656424
1105	40-41	0.31930881999576144
1105	42-43	0.31358119002712925
1105	44-45	0.34351321317422645
1105	46-47	0.31587269470468016
1105	48-49	0.1886983267172866
1105	50-51	0.3403124180285104
1105	52-53	0.42088525724500414
1105	54-55	0.29742988462855635
1105	56-57	0.3693642635785821
1105	58-59	0.39580917656264347
1105	60-61	0.3243967213235237
1105	62-63	0.26383455473992967
1105	64-65	0.2969218124994413
1105	66-67	0.2729233641571298
1105	68-69	0.14871873283403403
1105	70-71	0.2707289092749434
1105	72-73	0.23777176514293785
1105	74-75	0.22775151458973397
1105	76-77	0.23637426469484524
1105	78-79	0.2076750999268313
1105	80-81	0.22179560200158477
1105	82-83	0.23191283019267672
1105	84-85	0.19833051121865708
1105	86-87	0.07783869734686277
1105	88-89	0.14209252675744466
1105	90-91	0.26094786090875033
1105	92-93	0.17981545087431527
1105	94-95	0.1340907615913558
1105	96-97	0.1150036226477269
1105	98-99	0.07845029126066905
1105	100	0.18576208312451215
1106	1	0.09684496194080339
1106	2	0.20183529113720056
1106	3	0.13939736197307084
1106	4	0.31152977344617483
1106	5	0.14224000670906634
1106	6	0.15792664556899894
1106	7	0.13065906552131423
1106	8	0.19743921526347918
1106	9	0.2959096081070882
1106	10-11	0.25272901740311227
1106	12-13	0.1814601276097676
1106	14-15	0.3461229911917343
1106	16-17	0.2953371310388633
1106	18-19	0.25216009436079645
1106	20-21	0.2500331385209478
1106	22-23	0.24552759165341342
1106	24-25	0.32037393546243464
1106	26-27	0.3957208095727154
1106	28-29	0.2898548248027737
1106	30-31	0.3495869013856492
1106	32-33	0.29733468561456533
1106	34-35	0.3077518172420497
1106	36-37	0.3357265587969849
1106	38-39	0.27580552353096266
1106	40-41	0.3425885351471436
1106	42-43	0.2896998127240593
1106	44-45	0.2939530240897952
1106	46-47	0.36333644222938233
1106	48-49	0.2438693358986086
1106	50-51	0.33224515747779293
1106	52-53	0.1994495214955876
1106	54-55	0.2559635800130451
1106	56-57	0.3607474153828676
1106	58-59	0.2847560563844951
1106	60-61	0.29921892133639005
1106	62-63	0.29709224457230476
1106	64-65	0.23831010988792656
1106	66-67	0.19661070258962354
1106	68-69	0.29945533643785893
1106	70-71	0.2860527022002515
1106	72-73	0.1883790635439837
1106	74-75	0.21303719179213232
1106	76-77	0.229242787936073
1106	78-79	0.17947992254242706
1106	80-81	0.16725456421831808
1106	82-83	0.1955477152396412
1106	84-85	0.22934912526281437
1106	86-87	0.21693363004722244
1106	88-89	0.1875791496892063
1106	90-91	0.2073508202255283
1106	92-93	0.25957565385097325
1106	94-95	0.2549263157178636
1106	96-97	0.25782848522307944
1106	98-99	0.21249751680092643
1106	100	0.2027684522142792
1107	1	0.02926234668198191
1107	2	0.08282944171270756
1107	3	0.038945006479014666
1107	4	0.17840739677678386
1107	5	0.08723939870436936
1107	6	0.12835426156161844
1107	7	0.09634147741304844
1107	8	0.06870222970882622
1107	9	0.14568145958622125
1107	10-11	0.24077415651039047
1107	12-13	0.09923283868173627
1107	14-15	0.20552668534440954
1107	16-17	0.13917095706565874
1107	18-19	0.17415393045111216
1107	20-21	0.12613509461881733
1107	22-23	0.29054212086908393
1107	24-25	0.28881771599370865
1107	26-27	0.18561507965258528
1107	28-29	0.15750090317247611
1107	30-31	0.34112980764809464
1107	32-33	0.18305693229674347
1107	34-35	0.16927340140876623
1107	36-37	0.24493378357002626
1107	38-39	0.2422242081442505
1107	40-41	0.17121800869893633
1107	42-43	0.1945590491540159
1107	44-45	0.2445689747785167
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2208	98-99	0.20320533651238293
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2209	6	0.1399519241729763
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2215	26-27	-0.977313022931817
2215	28-29	-0.8147507453967293
2215	30-31	-3.61196637538751
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2216	2	-0.7265628010060112
2216	3	-0.6861011247676281
2216	4	-0.5700102798054161
2216	5	-0.49616819284717906
2216	6	-0.4113242227646836
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2216	14-15	-0.8488720076506837
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2216	18-19	-1.1655599032729995
2216	20-21	-1.2465737389640523
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2216	26-27	-1.4809123363761145
2216	28-29	-1.3098097553206998
2216	30-31	-1.4361814346657198
2216	32-33	-1.5913462429341934
2216	34-35	-1.2545650309386644
2216	36-37	-1.3430167423393655
2216	38-39	-1.2842197780323872
2216	40-41	-1.1725877485258351
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2216	54-55	-1.3038867711189681
2216	56-57	-1.1628059949081333
2216	58-59	-1.082724565818964
2216	60-61	-1.1513038880559918
2216	62-63	-1.2671677882963053
2216	64-65	-1.1788931815373829
2216	66-67	-1.1536402486212367
2216	68-69	-1.1984153805608884
2216	70-71	-1.1302204354728644
2216	72-73	-1.0978593603001414
2216	74-75	-1.0961632415714533
2216	76-77	-1.0510073465141403
2216	78-79	-0.8805196675758538
2216	80-81	-1.0190409202663844
2216	82-83	-0.9836569280446454
2216	84-85	-1.0390292655325375
2216	86-87	-1.03182880490845
2216	88-89	-1.0859957305968102
2216	90-91	-1.1440507275359053
2216	92-93	-1.152670508500023
2216	94-95	-1.247027303687883
2216	96-97	-1.2257624329990584
2216	98-99	-1.2280346883417224
2216	100	-0.8439466535637337
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	6.0
3	18.0
4	25.0
5	14.0
6	26.0
7	114.0
8	314.0
9	534.0
10	811.0
11	1412.0
12	2622.0
13	3949.0
14	5808.0
15	7611.0
16	9320.0
17	10926.0
18	12619.0
19	14199.0
20	16497.0
21	18609.0
22	21200.0
23	24410.0
24	28267.0
25	32654.0
26	36276.0
27	40042.0
28	45557.0
29	54940.0
30	65023.0
31	75530.0
32	93090.0
33	121305.0
34	172660.0
35	247659.0
36	404346.0
37	704524.0
38	649153.0
39	233144.0
40	460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10296962396727	19.76916840446165	11.59795440630508	24.529907565265997
2	16.04482424768693	19.755311074384355	39.49972032382223	24.700144354106488
3	19.036101431982797	25.59102368468173	28.724557625542126	26.648317257793348
4	12.167543793485281	15.84757832500575	39.372461873030744	32.61241600847822
5	13.157654560031942	38.35478217881397	35.186099500248766	13.30146376090532
6	30.985647691873787	37.859671250067336	17.658821329767175	13.495859728291698
7	27.77879007302786	31.704453447710375	22.844870066182985	17.671886413078784
8	26.831812718280435	34.77047855088253	19.732978919271613	18.66472981156542
9	26.34826915599509	14.995260891788936	20.790852744769158	37.86561720744682
10-11	25.834694776163268	25.719837644080425	27.582221719421884	20.863245860334427
12-13	25.690361400731256	23.52007468470998	27.70264660455194	23.086917310006825
14-15	22.998412381522783	25.0399598183587	24.86776183821502	27.093865961903496
16-17	22.38664457092263	27.760479937559822	26.096121556802448	23.756753934715093
18-19	21.88674784852855	28.185551479244563	27.57393053877339	22.353770133453498
20-21	23.27503528193782	27.334323831386325	26.835694625975016	22.55494626070084
22-23	23.511880146138203	27.120288955372963	26.551593447040744	22.81623745144809
24-25	23.057084345080828	27.054200617149466	27.378366494884155	22.51034854288555
26-27	23.02511011442901	27.291044026440776	26.383461555890975	23.300384303239245
28-29	22.359861178298548	27.799801524833086	26.733270829870527	23.10706646699784
30-31	23.00215922831237	27.53094532115174	26.77683954701047	22.69005590352542
32-33	23.278028785956696	27.537040916217002	26.08243645929627	23.102493838530034
34-35	23.320735535613444	27.39868892481821	26.884409132019556	22.396166407548794
36-37	22.994852045427752	27.080304161590757	26.604394079028264	23.320449713953227
38-39	23.139030889943346	27.2293584552398	27.04350793996374	22.588102714853115
40-41	22.81608110812531	26.449096757192976	27.625316056151632	23.109506078530078
42-43	22.78551320154633	26.6541530021306	27.054569138260675	23.5057646580624
44-45	22.84634068920204	27.5174485892399	26.97619011018669	22.660020611371372
46-47	22.939608612756988	27.27268981545971	26.590605939158074	23.197095632625228
48-49	23.440749791601295	27.10771122753969	26.593465630853785	22.858073350005228
50-51	23.085264856058902	26.884910510251625	26.75998618114741	23.269838452542064
52-53	22.686386209421354	27.3642095687433	26.59551825211991	23.353885969715435
54-55	22.59735291479522	27.249957449748706	26.69712113081928	23.45556850463679
56-57	23.49205051274823	26.894255790474368	26.347302162919128	23.266391533858275
58-59	22.6091221497739	27.175965433988697	27.096206248727267	23.118706167510137
60-61	22.598564487831997	27.730299423440275	26.55518440228697	23.11595168644076
62-63	22.88497518558715	27.176880595602952	26.270713605118463	23.667430613691433
64-65	22.22000375238327	27.353622190783334	26.67012747961543	23.75624657721796
66-67	22.651200992125652	27.032429013843775	26.632444289992645	23.683925704037925
68-69	22.389440841142804	27.021297464297845	26.83349936531785	23.755762329241502
70-71	22.336000501995624	26.782163689870654	26.91805745441316	23.963778353720567
72-73	22.798430829693174	26.711896699827015	27.338008772509443	23.151663697970367
74-75	22.913361396044895	26.971230121629734	26.924562816886056	23.19084566543932
76-77	22.653545888627622	26.81891383574857	26.810642174096927	23.71689810152688
78-79	22.561618835323554	26.76434975947654	26.7740001720911	23.900031233108805
80-81	22.194498699515712	27.10578782853193	27.322926078552612	23.376787393399745
82-83	22.852141136443187	26.325124929269993	28.12792199488795	22.694811939398864
84-85	22.979797299535026	26.740146205859233	27.1152715289023	23.16478496570344
86-87	21.909222588138206	27.257879707466522	27.51592944094718	23.3169682634481
88-89	22.82504630898751	26.277929984296932	27.2422502761109	23.654773430604656
90-91	22.540313635009475	26.35668692912787	27.31581549539499	23.787183940467663
92-93	22.80265968016272	26.456344755701068	27.307500086359425	23.433495477776788
94-95	21.83927953166706	26.421878354813384	27.93076100933386	23.808081104185696
96-97	22.142267221823257	26.29440581912958	27.557848706941083	24.005478252106084
98-99	21.63636170604867	26.80661527006194	27.405097901325554	24.151925122563835
100	22.306475426789998	26.21158286339384	26.77274606173812	24.70919564807804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	346.0
1	253.5
2	221.5
3	302.5
4	282.0
5	253.0
6	255.5
7	246.5
8	279.0
9	293.5
10	283.5
11	295.5
12	300.5
13	318.0
14	342.5
15	446.5
16	558.0
17	630.0
18	871.5
19	1193.0
20	1548.0
21	2091.5
22	2911.0
23	4028.0
24	5365.0
25	7108.5
26	9484.5
27	11882.5
28	15279.0
29	21593.0
30	29928.0
31	37652.5
32	48063.5
33	56685.0
34	61386.5
35	69433.0
36	77065.0
37	88006.5
38	93793.5
39	90020.0
40	85556.5
41	81101.5
42	81153.5
43	87011.5
44	95809.5
45	112012.5
46	127450.5
47	137033.5
48	143912.5
49	142956.0
50	136943.0
51	139020.5
52	140074.5
53	134405.5
54	127944.0
55	112331.0
56	99614.0
57	84485.5
58	69267.5
59	66022.5
60	56128.5
61	41371.0
62	32317.5
63	26223.0
64	19106.5
65	11394.0
66	7470.0
67	5208.5
68	3665.5
69	2499.0
70	1716.5
71	1222.5
72	804.0
73	468.5
74	286.5
75	169.5
76	94.5
77	61.5
78	34.0
79	20.5
80	21.5
81	23.0
82	19.5
83	15.5
84	14.0
85	12.0
86	10.0
87	9.0
88	8.5
89	6.5
90	5.5
91	5.5
92	6.0
93	5.5
94	3.5
95	2.5
96	2.5
97	2.0
98	1.5
99	1.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05621618195790124
2	0.00760534592440603
3	0.008239124751439866
4	0.009411615581452462
5	0.0033273388419276383
6	0.0026935600148938026
7	9.506682405507538E-4
8	0.001394313419474439
9	5.704009443304524E-4
10-11	4.911785909512227E-4
12-13	2.852004721652262E-4
14-15	1.5844470675845898E-4
16-17	4.59489649599531E-4
18-19	0.001394313419474439
20-21	7.446901217647571E-4
22-23	0.0010140461232541374
24-25	0.0011883353006884422
26-27	0.0029787604870590285
28-29	0.0036917616674720937
30-31	0.004737496732077923
32-33	0.005640631560601139
34-35	0.003073827311114104
36-37	0.007827168513867873
38-39	0.005767387326007906
40-41	0.005814920738035444
42-43	0.014497690668398996
44-45	0.019662988108724758
46-47	0.017888407393030017
48-49	0.021073145998875043
50-51	0.017904251863705863
52-53	0.024321262487423452
54-55	0.018442963866684627
56-57	0.01494133584732268
58-59	0.017508140096809715
60-61	0.01567018149841159
62-63	0.011138662885119666
64-65	0.010996062649037052
66-67	0.013610400310551626
68-69	0.00502269720424315
70-71	0.008524325223605092
72-73	0.007906390867247102
74-75	0.011439707827960737
76-77	0.010251372527272295
78-79	0.011709063829450118
80-81	0.00988694970172784
82-83	0.006005054386145595
84-85	0.010441506175382446
86-87	0.00873030334239109
88-89	0.00760534592440603
90-91	0.013150910660952093
92-93	0.008175746868736481
94-95	0.007209234157509883
96-97	0.009158104050638929
98-99	0.008825370166446165
100	0.2625111901574148
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3155675.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.00172605620644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97093201211473	56.07072481127473
2	13.302374983729623	18.889831689834082
3	4.0456334300023595	8.617408695625752
4	1.6060106437709702	4.561181110895127
5	0.7878246866278703	2.7968456290134367
6	0.4100267621927198	1.746756470695247
7	0.24907997741428434	1.2379575825718572
8	0.15303468032414305	0.8692581159579145
9	0.10160467516184632	0.649269658068413
>10	0.363682685672876	4.007254606218115
>50	0.008319790218905429	0.379665684985998
>100	0.0014168783591487647	0.14241192501431216
>500	5.8794410493245305E-5	0.03143401984490597
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.337788270338359E-5
2	0.0	0.0	0.0	0.0	6.337788270338359E-5
3	0.0	0.0	0.0	0.0	6.337788270338359E-5
4	0.0	0.0	0.0	0.0	6.337788270338359E-5
5	0.0	0.0	0.0	0.0	6.337788270338359E-5
6	0.0	0.0	0.0	0.0	6.337788270338359E-5
7	0.0	0.0	0.0	0.0	6.337788270338359E-5
8	0.0	0.0	0.0	0.0	6.337788270338359E-5
9	0.0	0.0	0.0	0.0	6.337788270338359E-5
10-11	6.337788270338359E-5	0.0	0.0	3.168894135169179E-5	9.506682405507538E-5
12-13	6.337788270338359E-5	0.0	0.0	6.337788270338359E-5	2.2182258946184256E-4
14-15	7.922235337922949E-5	0.0	0.0	6.337788270338359E-5	2.6935600148938023E-4
16-17	1.2675576540676717E-4	3.168894135169179E-5	0.0	7.922235337922949E-5	3.9611176689614743E-4
18-19	1.9013364811015077E-4	3.168894135169179E-5	0.0	1.1091129473092128E-4	4.119562375719933E-4
20-21	1.9013364811015077E-4	3.168894135169179E-5	0.0	1.2675576540676717E-4	4.59489649599531E-4
22-23	2.2182258946184256E-4	3.168894135169179E-5	0.0	1.4260023608261306E-4	4.753341202753769E-4
24-25	3.01044942841072E-4	3.168894135169179E-5	0.0	2.5351153081353434E-4	4.753341202753769E-4
26-27	3.4857835486860975E-4	3.168894135169179E-5	0.0	6.1793435635799E-4	5.070230616270687E-4
28-29	3.4857835486860975E-4	3.168894135169179E-5	0.0	0.0023766706013768845	5.070230616270687E-4
30-31	3.8026729622030154E-4	3.168894135169179E-5	0.0	0.009506682405507538	5.070230616270687E-4
32-33	3.8026729622030154E-4	3.168894135169179E-5	0.0	0.02325968295214178	5.070230616270687E-4
34-35	3.9611176689614743E-4	3.168894135169179E-5	0.0	0.045774675782518794	5.704009443304523E-4
36-37	4.119562375719933E-4	3.168894135169179E-5	0.0	0.08627314282998091	5.704009443304523E-4
38-39	4.119562375719933E-4	3.168894135169179E-5	0.0	0.1490014022356548	5.704009443304523E-4
40-41	4.119562375719933E-4	3.168894135169179E-5	0.0	0.2186536953266734	5.704009443304523E-4
42-43	4.119562375719933E-4	3.168894135169179E-5	0.0	0.28927250112891856	5.862454150062982E-4
44-45	4.119562375719933E-4	3.168894135169179E-5	0.0	0.37459497571834866	6.337788270338358E-4
46-47	4.119562375719933E-4	3.168894135169179E-5	0.0	0.48387428996965787	6.813122390613736E-4
48-49	4.119562375719933E-4	3.168894135169179E-5	0.0	0.6135137490394289	7.288456510889113E-4
50-51	4.119562375719933E-4	3.168894135169179E-5	0.0	0.7543869378183748	7.922235337922949E-4
52-53	4.911785909512227E-4	3.168894135169179E-5	0.0	0.8919644766967447	8.872903578473702E-4
54-55	5.070230616270687E-4	3.168894135169179E-5	0.0	1.041520435406054	9.18979299199062E-4
56-57	5.070230616270687E-4	3.168894135169179E-5	0.0	1.2259025406608728	9.506682405507538E-4
58-59	5.070230616270687E-4	3.168894135169179E-5	0.0	1.4498641336639546	9.982016525782915E-4
60-61	5.070230616270687E-4	3.168894135169179E-5	0.0	1.6857249241445966	0.0010140461232541374
62-63	5.070230616270687E-4	3.168894135169179E-5	0.0	1.9334056897494196	0.0010457350646058293
64-65	5.070230616270687E-4	3.168894135169179E-5	0.0	2.192050829061928	0.001061579535281675
66-67	5.070230616270687E-4	3.168894135169179E-5	0.0	2.4898793443558036	0.0011091129473092126
68-69	5.070230616270687E-4	3.168894135169179E-5	0.0	2.8272715029272657	0.0011408018886609045
70-71	5.070230616270687E-4	3.168894135169179E-5	0.0	3.196130780260958	0.0011566463593367503
72-73	5.070230616270687E-4	3.168894135169179E-5	0.0	3.5832270433425495	0.00123586871271598
74-75	5.070230616270687E-4	3.168894135169179E-5	0.0	3.959105421185642	0.0012992465954193635
76-77	5.070230616270687E-4	3.168894135169179E-5	0.0	4.390502824276898	0.0012992465954193635
78-79	5.070230616270687E-4	3.168894135169179E-5	0.0	4.862636361475754	0.0013309355367710552
80-81	5.070230616270687E-4	3.168894135169179E-5	0.0	5.380211840572936	0.0013467800074469013
82-83	5.070230616270687E-4	3.168894135169179E-5	0.0	5.918131620017904	0.0014101578901502849
84-85	5.070230616270687E-4	3.168894135169179E-5	0.0	6.437244012770643	0.0014260023608261307
86-87	5.070230616270687E-4	3.168894135169179E-5	0.0	6.9812956023671635	0.0015527581262328978
88	5.070230616270687E-4	3.168894135169179E-5	0.0	7.424560513994629	0.001679513891639665
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3025	0.0	35.507473	94
CAACGCA	5045	0.0	34.09385	5
GTACTAG	2935	0.0	33.64458	1
GTATCAA	5900	0.0	32.51712	1
ATCAACG	5430	0.0	31.67852	3
TAGGCAT	3895	0.0	29.802013	5
CTGTGCG	3950	0.0	29.62453	9
GGTATCA	3795	0.0	28.870085	1
TAGTACT	4005	0.0	28.633272	4
GCATCGT	3205	0.0	28.592743	8
CTAACGC	1415	0.0	27.900148	3
CTAGGCA	4220	0.0	27.73132	4
ATACGGG	1940	0.0	27.617653	3
AACGCAG	6735	0.0	27.492098	6
GTACATG	12205	0.0	26.737799	1
TATCAAC	7095	0.0	26.695816	2
TACGGGC	1990	0.0	26.68757	4
TCTAACG	1470	0.0	26.536968	2
GGCGTCG	1970	0.0	26.479267	8
TACATGG	12285	0.0	26.091541	2
>>END_MODULE