FastQCFastQC Report
Mon 16 Jan 2017
ERR1146674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155675
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.2713207158531851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72250.22895260126597322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.1498253147107988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.048.1872331
GTATCAA100700.040.173121
GGGCACC29400.039.34682594
CTGTGCG36450.033.9146549
GTGGTAT16800.033.879941
TAGTACT37450.033.78464
CAACGCA119950.032.8351145
ATCAACG121000.032.49673
CTAGTAC43200.030.4854493
TATCAAC133900.030.2123432
GTACTGT43100.029.9867616
AACGCAG136700.029.7043046
TAGGCAT37750.029.5070785
ACTGTGC48300.029.1918778
TACTGTG49650.028.397697
CTAGGCA42650.028.3421274
TCAACGC142550.027.9469574
CCTAGTA49850.026.7047082
ACGCAGA146850.026.6595067
CTAACGC12600.026.5036773
Produced by FastQC (version 0.11.5)