FastQCFastQC Report
Mon 16 Jan 2017
ERR1146673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4050050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC49550.040.12898694
GTACTAG50250.034.438731
CAACGCA62700.033.500265
CTGTGCG66900.031.6066519
TAGTACT66900.031.1910044
GTATCAA81550.030.5623871
ATCAACG70250.029.9715753
TAGGCAT64450.029.5284275
ACTGTGC75000.028.88238
GCATCGT54800.028.8105038
CTAACGC28150.027.5475793
GTACTGT77150.027.5296066
CTAGGCA74400.027.1623944
TACTGTG82800.026.9559947
CTAGTAC82250.026.7987673
TCTAACG29800.026.4957542
TAACGCC29800.026.3373894
ACGCAGA79800.026.2617747
TATCAAC94300.026.0160922
AACGCAG87100.025.7873786
Produced by FastQC (version 0.11.5)