##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146673_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4050050
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46298638288416	31.0	31.0	34.0	28.0	34.0
2	31.608689028530513	33.0	31.0	34.0	28.0	34.0
3	31.7341255046234	33.0	31.0	34.0	28.0	34.0
4	35.18357353612918	37.0	35.0	37.0	32.0	37.0
5	35.32663053542549	37.0	35.0	37.0	32.0	37.0
6	35.43425365118949	37.0	35.0	37.0	33.0	37.0
7	35.400108146813004	37.0	35.0	37.0	32.0	37.0
8	35.37660646164862	37.0	35.0	37.0	32.0	37.0
9	36.95636325477463	39.0	37.0	39.0	33.0	39.0
10-11	36.80765076974358	39.0	37.0	39.0	32.0	39.0
12-13	36.751760965914	39.0	37.0	39.0	32.0	39.0
14-15	37.95934260070864	40.0	38.0	41.0	32.0	41.0
16-17	37.962750830236665	40.0	38.0	41.0	32.0	41.0
18-19	37.895494253157366	40.0	38.0	41.0	32.0	41.0
20-21	37.9360860977025	40.0	38.0	41.0	32.0	41.0
22-23	37.852918729398404	40.0	38.0	41.0	32.0	41.0
24-25	37.78050604313527	40.0	37.0	41.0	32.0	41.0
26-27	37.79390489006309	40.0	37.0	41.0	32.0	41.0
28-29	37.752721571338625	40.0	37.0	41.0	32.0	41.0
30-31	37.600716410908504	40.0	37.0	41.0	31.0	41.0
32-33	37.588163356007954	40.0	37.0	41.0	31.0	41.0
34-35	37.517798669152235	40.0	37.0	41.0	31.0	41.0
36-37	37.391919359020264	40.0	37.0	41.0	31.0	41.0
38-39	37.28450056172146	40.0	36.0	41.0	30.0	41.0
40-41	37.19139529141616	40.0	36.0	41.0	30.0	41.0
42-43	37.013265762151086	39.5	36.0	41.0	30.0	41.0
44-45	36.84594622288614	39.0	35.0	41.0	30.0	41.0
46-47	36.825576844730314	39.0	35.0	41.0	30.0	41.0
48-49	36.653239836545225	39.0	35.0	41.0	30.0	41.0
50-51	36.44915791163072	39.0	35.0	41.0	29.5	41.0
52-53	36.20452772187998	38.5	35.0	40.0	29.0	41.0
54-55	35.94220861470846	38.0	35.0	40.0	29.0	41.0
56-57	35.66826718188664	37.5	34.0	40.0	28.0	41.0
58-59	35.323434278589154	37.0	34.0	40.0	28.0	41.0
60-61	35.037952988234714	36.5	34.0	39.5	28.0	41.0
62-63	34.68307292502562	36.0	33.5	39.0	27.0	41.0
64-65	34.505094134640316	35.0	33.5	39.0	27.0	41.0
66-67	34.22386291527265	35.0	33.0	38.5	27.0	40.5
68-69	34.064693769212724	35.0	33.0	37.5	27.0	40.0
70-71	33.96101813557857	35.0	33.0	37.0	27.0	40.0
72-73	33.715795854372175	35.0	33.0	36.5	27.0	39.0
74-75	33.467844100690115	35.0	33.0	36.0	26.5	39.0
76-77	33.219612350464814	35.0	33.0	36.0	26.0	39.0
78-79	32.98241404427105	35.0	33.0	35.0	26.0	37.0
80-81	32.82215194874137	35.0	33.0	35.0	26.0	37.0
82-83	32.60091356896828	35.0	33.0	35.0	26.0	36.5
84-85	32.456093875384255	35.0	33.0	35.0	25.0	36.0
86-87	32.3212492438365	35.0	33.0	35.0	25.0	36.0
88-89	32.22672855890668	35.0	33.0	35.0	25.0	36.0
90-91	32.10089640868631	35.0	32.0	35.0	25.0	35.0
92-93	31.961702448117926	35.0	32.0	35.0	25.0	35.0
94-95	31.905148084591545	35.0	32.0	35.0	25.0	35.0
96-97	31.7976060789373	35.0	32.0	35.0	24.0	35.0
98-99	31.70263144899446	35.0	32.0	35.0	24.0	35.0
100	30.71165615239318	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3370239023234589
1101	2	0.3987516742913364
1101	3	0.4714163950060808
1101	4	0.47085130802375375
1101	5	0.39240514562128226
1101	6	0.348017749541242
1101	7	0.3771307591026698
1101	8	0.33612908983039347
1101	9	0.5138556550960445
1101	10-11	0.5443393383757922
1101	12-13	0.46462522816237595
1101	14-15	0.6839701959770039
1101	16-17	0.6777521219352138
1101	18-19	0.6743445887595385
1101	20-21	0.6700744755993782
1101	22-23	0.6361872006439668
1101	24-25	0.6753608199849452
1101	26-27	0.6817934102006404
1101	28-29	0.6626504625804799
1101	30-31	0.6831731228806461
1101	32-33	0.6312986176889623
1101	34-35	0.673583675326519
1101	36-37	0.6740143830226444
1101	38-39	0.628791510633171
1101	40-41	0.6350780771870603
1101	42-43	0.6400159364253781
1101	44-45	0.5827291805214614
1101	46-47	0.6455829875860459
1101	48-49	0.6183408326869753
1101	50-51	0.63730144319738
1101	52-53	0.6100521562192682
1101	54-55	0.5564829735977312
1101	56-57	0.5444930640869217
1101	58-59	0.5869601916466891
1101	60-61	0.5526188900935693
1101	62-63	0.5457226038786303
1101	64-65	0.5221145753945748
1101	66-67	0.5297930750943252
1101	68-69	0.5054669624001349
1101	70-71	0.5274443748138253
1101	72-73	0.5324470193754394
1101	74-75	0.43749175866526
1101	76-77	0.5232265988882787
1101	78-79	0.5083527805998642
1101	80-81	0.427625280808833
1101	82-83	0.39354337069590173
1101	84-85	0.440091347211812
1101	86-87	0.4561120230905473
1101	88-89	0.4207333512855058
1101	90-91	0.4120091064904159
1101	92-93	0.4807652951297676
1101	94-95	0.4589754858347739
1101	96-97	0.4838171159663496
1101	98-99	0.48275550799074196
1101	100	0.4734580579578953
1102	1	0.31861198436628513
1102	2	0.4291566489933878
1102	3	0.46436590313858517
1102	4	0.5680593053467291
1102	5	0.4706006766299069
1102	6	0.456339332524891
1102	7	0.4325289193916575
1102	8	0.415597359147732
1102	9	0.5424493102908059
1102	10-11	0.6665565419257007
1102	12-13	0.5670204294842804
1102	14-15	0.7707518028381699
1102	16-17	0.7578313614873338
1102	18-19	0.758189454358785
1102	20-21	0.7025037061144985
1102	22-23	0.7271480227329548
1102	24-25	0.7898102413577917
1102	26-27	0.7095455869204841
1102	28-29	0.7489523105048832
1102	30-31	0.7684574909329598
1102	32-33	0.660409037242438
1102	34-35	0.7027527056016964
1102	36-37	0.7559787836938909
1102	38-39	0.7935174234316946
1102	40-41	0.7810952671081353
1102	42-43	0.7525576182947304
1102	44-45	0.7579358354953101
1102	46-47	0.6726734949506934
1102	48-49	0.6718189700416204
1102	50-51	0.7171006177057038
1102	52-53	0.6128309334066842
1102	54-55	0.6424564689155332
1102	56-57	0.656666581495152
1102	58-59	0.5870293652727412
1102	60-61	0.5740928374867522
1102	62-63	0.6505141037106412
1102	64-65	0.6115760996840081
1102	66-67	0.5803744099656356
1102	68-69	0.5262526145857294
1102	70-71	0.5167128447501881
1102	72-73	0.5627134916959449
1102	74-75	0.5632465197851104
1102	76-77	0.5657209657153501
1102	78-79	0.5359994693028085
1102	80-81	0.578357333799687
1102	82-83	0.5344314455407186
1102	84-85	0.5743113383530769
1102	86-87	0.5590524620741419
1102	88-89	0.5308535613679908
1102	90-91	0.5715308215653678
1102	92-93	0.5557301286967551
1102	94-95	0.5860741191613563
1102	96-97	0.5979898710403297
1102	98-99	0.5602114805643161
1102	100	0.4942303408065811
1103	1	0.2509485476767921
1103	2	0.33770857253616526
1103	3	0.32469231579009517
1103	4	0.46014951933965875
1103	5	0.30935530139013423
1103	6	0.33936465011044703
1103	7	0.3343062616801973
1103	8	0.37370415888581476
1103	9	0.45482790860016564
1103	10-11	0.524872961564725
1103	12-13	0.4406976142817385
1103	14-15	0.5598850789746805
1103	16-17	0.5889168026766356
1103	18-19	0.6352330027641386
1103	20-21	0.5151661125507445
1103	22-23	0.5935973925576761
1103	24-25	0.5599067299038865
1103	26-27	0.5215477792061591
1103	28-29	0.5655241006165497
1103	30-31	0.5364975806626902
1103	32-33	0.517482172678335
1103	34-35	0.5395485863427751
1103	36-37	0.5210964873526436
1103	38-39	0.6306746560648833
1103	40-41	0.5478715064261905
1103	42-43	0.5014978757070594
1103	44-45	0.4774667338783658
1103	46-47	0.49276092714881514
1103	48-49	0.5002806098717443
1103	50-51	0.4639753727707685
1103	52-53	0.47526378653986967
1103	54-55	0.5178726362757331
1103	56-57	0.5031876283035075
1103	58-59	0.5241171668299813
1103	60-61	0.5285310252890412
1103	62-63	0.48273937358037244
1103	64-65	0.48572500280845077
1103	66-67	0.5454550972244832
1103	68-69	0.49563996582904224
1103	70-71	0.5055220934713134
1103	72-73	0.5257760538247709
1103	74-75	0.5664590670263294
1103	76-77	0.4740079432032118
1103	78-79	0.392512196372067
1103	80-81	0.3518562115119579
1103	82-83	0.39429620093217466
1103	84-85	0.41922516788212505
1103	86-87	0.35522995605858654
1103	88-89	0.49093294082692296
1103	90-91	0.4492164880781573
1103	92-93	0.37645174116743974
1103	94-95	0.40699911783469034
1103	96-97	0.3915531251485227
1103	98-99	0.4255304999275289
1103	100	0.49623328413354884
1104	1	0.16893173067317235
1104	2	0.2411811773796586
1104	3	0.16667761652790247
1104	4	0.318793563881151
1104	5	0.20913763625605952
1104	6	0.22380175995341745
1104	7	0.21068956770891134
1104	8	0.2685214756867538
1104	9	0.25559840074242146
1104	10-11	0.3926092183232015
1104	12-13	0.30934002830019125
1104	14-15	0.37004820235896574
1104	16-17	0.4355518134311964
1104	18-19	0.39884507009936243
1104	20-21	0.36019776666790904
1104	22-23	0.4129829330706869
1104	24-25	0.4594500326758606
1104	26-27	0.41961583017324955
1104	28-29	0.37170918932174146
1104	30-31	0.37979537706571875
1104	32-33	0.43993241264738714
1104	34-35	0.4662506251642995
1104	36-37	0.44384127689455255
1104	38-39	0.3944632915992514
1104	40-41	0.4334577634646877
1104	42-43	0.47906738725232856
1104	44-45	0.48952759654353173
1104	46-47	0.43495665043979415
1104	48-49	0.3355868509625637
1104	50-51	0.3204042375950209
1104	52-53	0.4010999196198384
1104	54-55	0.4200668582793057
1104	56-57	0.3964641331341241
1104	58-59	0.410907334668309
1104	60-61	0.35947351715042686
1104	62-63	0.39910595328581167
1104	64-65	0.40561925789634756
1104	66-67	0.3770551292749289
1104	68-69	0.3985453877381815
1104	70-71	0.40630732386185286
1104	72-73	0.4108029089916698
1104	74-75	0.3295281292623429
1104	76-77	0.34869513909558947
1104	78-79	0.3032749300678077
1104	80-81	0.26088185660244534
1104	82-83	0.30204825567122384
1104	84-85	0.3679324560401369
1104	86-87	0.28044096555698417
1104	88-89	0.31064722451036175
1104	90-91	0.3345755057752555
1104	92-93	0.3334301644150379
1104	94-95	0.3551576391376763
1104	96-97	0.3777784901422869
1104	98-99	0.36961096354747625
1104	100	0.4816017629567817
1105	1	0.16961014052636614
1105	2	0.28410187986228763
1105	3	0.24222250982318627
1105	4	0.34841532467694947
1105	5	0.2158887626324386
1105	6	0.23590419482824387
1105	7	0.3152617927505901
1105	8	0.2959689347118726
1105	9	0.396473020761583
1105	10-11	0.40199377598468544
1105	12-13	0.2931504673466634
1105	14-15	0.5005317354029657
1105	16-17	0.4639950263868755
1105	18-19	0.48363991920781757
1105	20-21	0.38733697738273065
1105	22-23	0.41870502327795833
1105	24-25	0.4010437867780041
1105	26-27	0.3242771007072065
1105	28-29	0.4127099614283267
1105	30-31	0.4227084049948928
1105	32-33	0.38841788991158666
1105	34-35	0.4448456237327534
1105	36-37	0.4634075380071536
1105	38-39	0.4703471055784121
1105	40-41	0.34035612757031686
1105	42-43	0.3547867280572845
1105	44-45	0.3381587611221306
1105	46-47	0.39815900128213144
1105	48-49	0.26812380510025235
1105	50-51	0.39585879338566343
1105	52-53	0.37013707582827493
1105	54-55	0.3933508591351398
1105	56-57	0.40976405675034755
1105	58-59	0.44029953070689487
1105	60-61	0.43447039048049874
1105	62-63	0.41130379035374887
1105	64-65	0.3772917317279294
1105	66-67	0.3763351166235438
1105	68-69	0.36586677736467266
1105	70-71	0.39472546660957164
1105	72-73	0.3407777767671263
1105	74-75	0.3434768239680892
1105	76-77	0.27969694998011363
1105	78-79	0.3719882304565445
1105	80-81	0.37192646927264406
1105	82-83	0.36202713902111583
1105	84-85	0.30942928337457687
1105	86-87	0.28114428540859393
1105	88-89	0.303700900905703
1105	90-91	0.3281476150526288
1105	92-93	0.28168863510650866
1105	94-95	0.29339148924150393
1105	96-97	0.25707311340699235
1105	98-99	0.22879329543341242
1105	100	0.4371995357341838
1106	1	0.0653086135207559
1106	2	0.15994726739970844
1106	3	0.12920346627711154
1106	4	0.23387657093490333
1106	5	0.09655541252804056
1106	6	0.16295760228969414
1106	7	0.14658804869383601
1106	8	0.12535964186389492
1106	9	0.2534361160296328
1106	10-11	0.2459275135007175
1106	12-13	0.1745586080223731
1106	14-15	0.33955339523541994
1106	16-17	0.23577623943812398
1106	18-19	0.23263478726144626
1106	20-21	0.15007615375564853
1106	22-23	0.24998436216251463
1106	24-25	0.2775427427791044
1106	26-27	0.318489573349396
1106	28-29	0.28199526242704565
1106	30-31	0.33592857555663613
1106	32-33	0.2134337385694849
1106	34-35	0.2576816530150339
1106	36-37	0.2557925094544089
1106	38-39	0.2499619580349588
1106	40-41	0.2902069489332959
1106	42-43	0.20287218502691218
1106	44-45	0.25510688821326966
1106	46-47	0.28003367110175503
1106	48-49	0.23400692753742902
1106	50-51	0.2736346983943392
1106	52-53	0.2018186687441741
1106	54-55	0.2523650977706424
1106	56-57	0.23878043369684576
1106	58-59	0.19550895940051305
1106	60-61	0.211629204095253
1106	62-63	0.26043286886749684
1106	64-65	0.21571315508834488
1106	66-67	0.1686032356645697
1106	68-69	0.1944338574381277
1106	70-71	0.23120656142479845
1106	72-73	0.1755938820223406
1106	74-75	0.21562658120689093
1106	76-77	0.2152244838786359
1106	78-79	0.1767403121651796
1106	80-81	0.1895238882054997
1106	82-83	0.1947508440851351
1106	84-85	0.20080421733849363
1106	86-87	0.21945099467377105
1106	88-89	0.18609178415392336
1106	90-91	0.19399790812702378
1106	92-93	0.21551671490324864
1106	94-95	0.2063524210419594
1106	96-97	0.21254240977689065
1106	98-99	0.18297230979782952
1106	100	0.2929546110782013
1107	1	0.1473202283563566
1107	2	0.1958098575610343
1107	3	0.138955133875303
1107	4	0.24429757402975838
1107	5	0.12332270073014229
1107	6	0.1797771610369665
1107	7	0.13989368439980865
1107	8	0.12416066178928276
1107	9	0.22191837773057443
1107	10-11	0.29664455233586295
1107	12-13	0.18663728005024893
1107	14-15	0.32692002207570425
1107	16-17	0.16764133777815715
1107	18-19	0.17355476399645653
1107	20-21	0.16159505278126574
1107	22-23	0.324112183390433
1107	24-25	0.3328538616564316
1107	26-27	0.21951248886743002
1107	28-29	0.2805476093026087
1107	30-31	0.3197663665425807
1107	32-33	0.23708056480240458
1107	34-35	0.291019372717237
1107	36-37	0.29754158560437816
1107	38-39	0.23638525482643047
1107	40-41	0.25409145916346176
1107	42-43	0.22962959443662356
1107	44-45	0.29727626092887505
1107	46-47	0.20718590417565252
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2216	56-57	-1.2721269365420085
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2216	66-67	-1.1256553659971473
2216	68-69	-1.1602881503501123
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2216	72-73	-1.0286785005964987
2216	74-75	-1.0619627805803944
2216	76-77	-1.1624035903898573
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2216	80-81	-1.0086637729773749
2216	82-83	-1.0019005548821482
2216	84-85	-1.0775121042324152
2216	86-87	-0.9920304868077139
2216	88-89	-1.0268827868351593
2216	90-91	-1.162894343086677
2216	92-93	-1.1684441952117517
2216	94-95	-1.1307774802040491
2216	96-97	-1.2194922541907687
2216	98-99	-1.136448432544018
2216	100	-0.6945474903748021
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9.0
3	21.0
4	36.0
5	29.0
6	43.0
7	153.0
8	461.0
9	658.0
10	1045.0
11	1644.0
12	2893.0
13	4454.0
14	6385.0
15	8673.0
16	10739.0
17	12410.0
18	14021.0
19	15809.0
20	18307.0
21	20748.0
22	24154.0
23	28314.0
24	32742.0
25	37958.0
26	43290.0
27	49844.0
28	58191.0
29	70482.0
30	83826.0
31	100897.0
32	125283.0
33	164322.0
34	234607.0
35	344990.0
36	584070.0
37	1017243.0
38	758652.0
39	172506.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.001597202166266	18.898175088439046	11.342012988601189	24.758214720793497
2	16.741087247523353	19.384003530111013	38.48112380797638	25.393785414389253
3	19.759885226916364	24.981616058354508	27.907490128971563	27.35100858575757
4	12.87742113276838	15.582269497591646	38.546700532934416	32.993608836705555
5	13.919684535810099	38.150178831355305	34.18062892833289	13.74950770450171
6	31.687855822381962	36.848464017695036	17.22473542259421	14.23894473732879
7	28.344248091553105	30.962728341408603	22.387266633926096	18.3057569331122
8	27.475574013762998	33.6844535418606	19.16012138301576	19.679851061360644
9	26.71495110651087	14.478615950309479	20.17766296924367	38.62876997393598
10-11	26.90719907101911	24.63917855413152	26.54274493556158	21.910877439287788
12-13	26.802146003761194	22.36142388240905	26.637653780907566	24.198776332922183
14-15	24.18283006732024	23.660897594964993	23.962264616812902	28.194007720901865
16-17	23.58794803393952	26.43754561696857	25.085554964064443	24.88895138502746
18-19	22.842412607485233	27.26557571343033	26.467743628047085	23.42426805103735
20-21	24.749901975599084	26.506847387118853	25.645269693028073	23.097980944253994
22-23	25.081892066299826	25.87300405642426	25.46671965437986	23.57838422289605
24-25	24.67578371045707	25.6837227286835	26.196352738039064	23.444140822820362
26-27	24.594962071271663	26.19201444184342	24.85436363405157	24.35865985283335
28-29	23.540940796125117	26.508856994104885	25.866924155872823	24.08327805389717
30-31	24.16506197225493	26.460105241285664	25.938170590343585	23.43666219611582
32-33	24.43335473356709	26.270482492962614	25.01829719986172	24.277865573608572
34-35	24.463680870935622	26.350666899594373	25.86316073431488	23.322491495155123
36-37	24.13674107757431	25.684461617194863	25.13837144626234	25.04042585896848
38-39	24.350751528756447	26.069759123863196	25.730172650183214	23.849316697197143
40-41	24.045077993106297	25.046267933994454	26.564893262129484	24.34376081076977
42-43	23.901402327453887	25.29900073997767	26.000899135227495	24.798697797340946
44-45	24.384772775823343	25.857349423774355	25.89670292490236	23.861174875499948
46-47	24.10236729557139	25.97117697002565	25.288040141635687	24.638415592767274
48-49	24.842571042456548	25.781341068990653	25.458777791168863	23.917310097383933
50-51	24.12644320962486	25.408429111015103	25.96733307987166	24.497794599488376
52-53	24.042644204445008	25.691462391494575	25.64315405986331	24.622739344197107
54-55	23.738113680684464	25.652692581864944	25.69846654148285	24.910727195967745
56-57	24.885909248956885	25.318577431565302	25.16859254488557	24.626920774592236
58-59	23.520621578731348	25.901352875137494	26.216505955841278	24.36151959028988
60-61	23.65848068980709	26.189779432029326	25.57013891564206	24.58160096252152
62-63	24.15956384440399	25.708458984720327	25.10951796292144	25.02245920795424
64-65	23.358723733579087	25.84138729077367	25.75776096279054	25.0421280128567
66-67	24.115326487976173	25.646580037832205	25.512216443673974	24.725877030517648
68-69	23.56313296054292	25.415401383469575	26.075492775230884	24.945972880756617
70-71	23.26130620755527	25.36683163451514	25.827930023078377	25.543932134851214
72-73	23.890515969569496	25.18929551707274	26.429379163053735	24.49080935030403
74-75	24.09235907865637	25.30608233380186	26.020690128616312	24.580868458925455
76-77	23.86156657447649	25.28523804820229	25.454452291584357	25.398743085736864
78-79	23.90656018206526	25.383803136012833	25.317067111622883	25.392569570299024
80-81	23.3443051812565	25.868760335109105	26.049950779168295	24.736983704466102
82-83	24.156227502599837	24.98091559935389	27.079884838003903	23.782972060042372
84-85	24.005482022915846	25.11990073093639	26.280842552350848	24.59377469379692
86-87	22.830461192828714	25.800272665471667	26.627356283294535	24.74190985840508
88-89	24.079520281580727	24.484635809634717	26.370400934980758	25.065442973803798
90-91	23.520527553633993	24.90274069486384	25.98638493200615	25.590346819496013
92-93	23.75419199685111	24.883700716179867	26.489925996640228	24.872181290328793
94-95	22.905805073900705	24.945289488210864	27.127376195590593	25.02152924229784
96-97	23.180949417726023	24.77328848984107	26.7018673264784	25.343894765954506
98-99	22.68757908086917	25.091612810187147	26.604002022889865	25.616806086053813
100	23.410573042296924	24.481635950997514	25.910404710132013	26.197386296573548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	104.0
2	90.5
3	110.5
4	114.0
5	109.5
6	94.0
7	107.5
8	117.0
9	111.5
10	105.0
11	103.0
12	96.0
13	102.5
14	117.5
15	142.0
16	156.0
17	167.5
18	218.0
19	291.5
20	383.0
21	510.5
22	715.5
23	981.5
24	1283.5
25	1692.0
26	2276.0
27	2957.5
28	3960.5
29	6027.0
30	9478.0
31	13410.5
32	19511.0
33	27264.5
34	35370.0
35	43637.5
36	53295.5
37	69338.5
38	82587.5
39	90981.0
40	94545.5
41	95132.0
42	100534.5
43	111929.0
44	128695.0
45	156511.0
46	184927.0
47	206752.5
48	224093.5
49	226128.5
50	217755.5
51	224060.0
52	229549.5
53	220499.5
54	208949.0
55	180888.0
56	157933.5
57	130771.5
58	102461.0
59	98571.0
60	82971.5
61	57698.5
62	42669.0
63	34805.5
64	25637.0
65	13443.5
66	7712.5
67	5315.5
68	3642.5
69	2464.5
70	1698.5
71	1109.0
72	675.5
73	419.0
74	253.5
75	168.0
76	109.5
77	71.0
78	45.0
79	32.0
80	37.5
81	29.0
82	16.5
83	11.5
84	15.0
85	14.5
86	12.5
87	10.5
88	8.0
89	7.5
90	5.0
91	6.5
92	9.0
93	6.0
94	3.5
95	6.0
96	5.5
97	3.0
98	2.5
99	2.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05794990185306354
2	0.007876445969802841
3	0.007901137022999716
4	0.009777657065962148
5	0.003580202713546746
6	0.0024937963728842855
7	7.160405427093493E-4
8	0.0014814631918124467
9	5.432031703312305E-4
10-11	5.061665905359194E-4
12-13	2.592560585671782E-4
14-15	1.2345526598437056E-4
16-17	5.432031703312305E-4
18-19	0.0018518289897655586
20-21	9.382600214812162E-4
22-23	0.001345662399229639
24-25	0.0013086258194343278
26-27	0.003740694559326428
28-29	0.00423451562326391
30-31	0.0052715398575326235
32-33	0.006172763299218528
34-35	0.0037653856125233025
36-37	0.008703596251898125
38-39	0.006703620942951322
40-41	0.006641893309959137
42-43	0.014901050604313527
44-45	0.021110850483327365
46-47	0.0194565499191368
48-49	0.02276515104751793
50-51	0.019394822286144614
52-53	0.026147825335489684
54-55	0.019863952296885225
56-57	0.01585165615239318
58-59	0.018468907791261837
60-61	0.01693806249305564
62-63	0.01199985185368082
64-65	0.012049233960074568
66-67	0.014567721386155728
68-69	0.005728324341674794
70-71	0.00918507178923717
72-73	0.00897519783706374
74-75	0.01199985185368082
76-77	0.01111097393859335
78-79	0.01266651028999642
80-81	0.01095048209281367
82-83	0.006827076208935692
84-85	0.01111097393859335
86-87	0.009851730225552772
88-89	0.008419649140134072
90-91	0.013975136109430748
92-93	0.009110998629646547
94-95	0.008036937815582523
96-97	0.010123331810718386
98-99	0.009827039172355896
100	0.2572313922050345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4050050.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.14140398546381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10872130723097	50.42905000120407
2	14.760200374873763	19.820411525515873
3	5.18270086553699	10.43921437646496
4	2.214712212535108	5.947955494934404
5	1.0938716208499204	3.67220382018593
6	0.5814265493874411	2.3422676904177844
7	0.33981330586891084	1.5970879714286366
8	0.2014633393308932	1.0821225163420864
9	0.1337144138732025	0.8079996132486176
>10	0.37873395055769327	3.637532221428242
>50	0.0042262696684579	0.18634784919023265
>100	4.157902866322063E-4	0.037806919639207
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	4.9382106393748225E-5	0.0
24-25	0.0	0.0	0.0	1.975284255749929E-4	0.0
26-27	0.0	0.0	0.0	6.049308033234158E-4	0.0
28-29	0.0	0.0	0.0	0.0036913124529326796	0.0
30-31	0.0	0.0	0.0	0.013901062949840125	0.0
32-33	0.0	0.0	0.0	0.031320600980234814	0.0
34-35	0.0	0.0	0.0	0.061098011135664994	0.0
36-37	0.0	0.0	0.0	0.11286280416291157	0.0
38-39	0.0	0.0	0.0	0.19230626782385402	0.0
40-41	0.0	0.0	0.0	0.28130516907198677	0.0
42-43	0.0	0.0	0.0	0.3755756101776522	0.0
44-45	0.0	0.0	0.0	0.48801866643621683	0.0
46-47	0.0	0.0	0.0	0.6270539869878149	0.0
48-49	0.0	0.0	0.0	0.791397637066209	0.0
50-51	0.0	0.0	0.0	0.9713460327650276	0.0
52-53	0.0	0.0	0.0	1.143553783286626	1.2345526598437056E-5
54-55	0.0	0.0	0.0	1.3371439858767176	2.4691053196874112E-5
56-57	0.0	0.0	0.0	1.5757459784447105	2.4691053196874112E-5
58-59	0.0	0.0	0.0	1.8580881717509659	2.4691053196874112E-5
60-61	0.0	0.0	0.0	2.150566042394538	2.4691053196874112E-5
62-63	0.0	0.0	0.0	2.466043629090999	2.4691053196874112E-5
64-65	0.0	0.0	0.0	2.792669226305848	2.4691053196874112E-5
66-67	0.0	0.0	0.0	3.166886828557672	2.4691053196874112E-5
68-69	0.0	0.0	0.0	3.589375439809385	2.4691053196874112E-5
70-71	0.0	0.0	0.0	4.053517857804225	2.4691053196874112E-5
72-73	0.0	0.0	0.0	4.5338576066962135	2.4691053196874112E-5
74-75	0.0	0.0	0.0	4.995703756743744	2.4691053196874112E-5
76-77	0.0	0.0	0.0	5.530376168195454	2.4691053196874112E-5
78-79	0.0	0.0	0.0	6.111368995444501	2.4691053196874112E-5
80-81	0.0	0.0	0.0	6.730163825137961	2.4691053196874112E-5
82-83	0.0	1.2345526598437056E-5	0.0	7.382205157961012	2.4691053196874112E-5
84-85	0.0	2.4691053196874112E-5	0.0	8.006827076208936	2.4691053196874112E-5
86-87	0.0	2.4691053196874112E-5	1.2345526598437056E-5	8.671769484327353	2.4691053196874112E-5
88	0.0	2.4691053196874112E-5	2.4691053196874112E-5	9.214256614115875	2.4691053196874112E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4955	0.0	40.128986	94
GTACTAG	5025	0.0	34.43873	1
CAACGCA	6270	0.0	33.50026	5
CTGTGCG	6690	0.0	31.606651	9
TAGTACT	6690	0.0	31.191004	4
GTATCAA	8155	0.0	30.562387	1
ATCAACG	7025	0.0	29.971575	3
TAGGCAT	6445	0.0	29.528427	5
ACTGTGC	7500	0.0	28.8823	8
GCATCGT	5480	0.0	28.810503	8
CTAACGC	2815	0.0	27.547579	3
GTACTGT	7715	0.0	27.529606	6
CTAGGCA	7440	0.0	27.162394	4
TACTGTG	8280	0.0	26.955994	7
CTAGTAC	8225	0.0	26.798767	3
TCTAACG	2980	0.0	26.495754	2
TAACGCC	2980	0.0	26.337389	4
ACGCAGA	7980	0.0	26.261774	7
TATCAAC	9430	0.0	26.016092	2
AACGCAG	8710	0.0	25.787378	6
>>END_MODULE