FastQCFastQC Report
Mon 16 Jan 2017
ERR1146673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4050050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.10380118763965876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC49350.040.9845694
GTATCAA105550.034.6319471
GGTATCA67000.033.703931
CTGTGCG68450.031.7265749
GTACTAG52500.031.6322121
TAGTACT68300.031.5451134
GTGGTAT16600.031.4578481
CAACGCA107600.031.1002165
TAGGCAT64850.030.22195
ATCAACG112650.029.816343
ACTGTGC76850.029.4769658
CTAACGC24100.029.2793563
TGAACCG5200.028.9229345
GTACTGT76550.028.6094576
TACTGTG83450.027.877117
TATCAAC133700.027.4107042
GAACCGT5500.027.343636
CTAGTAC82800.027.3276353
TAACGCC25850.027.2971944
CTAGGCA75750.026.9522114
Produced by FastQC (version 0.11.5)