##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146673_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4050050 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09947778422489 33.0 31.0 34.0 30.0 34.0 2 32.15575783015024 33.0 31.0 34.0 30.0 34.0 3 32.32812631942816 34.0 31.0 34.0 30.0 34.0 4 35.9371585535981 37.0 35.0 37.0 35.0 37.0 5 35.83893433414402 37.0 35.0 37.0 35.0 37.0 6 35.87938148911742 37.0 35.0 37.0 35.0 37.0 7 35.804818952852436 37.0 35.0 37.0 35.0 37.0 8 35.81068110270244 37.0 35.0 37.0 35.0 37.0 9 37.52566931272453 39.0 37.0 39.0 35.0 39.0 10-11 37.46904519697288 39.0 37.0 39.0 34.0 39.0 12-13 37.43675300304935 39.0 37.0 39.0 34.0 39.0 14-15 38.67571955901779 40.0 38.0 41.0 34.0 41.0 16-17 38.658969889260625 40.0 38.0 41.0 34.0 41.0 18-19 38.690428513228234 40.0 38.0 41.0 34.0 41.0 20-21 38.677802125899674 40.0 38.0 41.0 34.0 41.0 22-23 38.63522931815656 40.0 38.0 41.0 34.0 41.0 24-25 38.56216651646276 40.0 38.0 41.0 34.0 41.0 26-27 38.42894816113382 40.0 38.0 41.0 34.0 41.0 28-29 38.370301971580595 40.0 38.0 41.0 34.0 41.0 30-31 38.268472241083444 40.0 38.0 41.0 34.0 41.0 32-33 38.162462932556394 40.0 37.0 41.0 33.0 41.0 34-35 38.07803385143393 40.0 37.0 41.0 33.0 41.0 36-37 38.08025888569277 40.0 37.0 41.0 33.0 41.0 38-39 37.99608066567079 40.0 37.0 41.0 33.0 41.0 40-41 37.88804595005 40.0 37.0 41.0 33.0 41.0 42-43 37.76210059135072 40.0 37.0 41.0 33.0 41.0 44-45 37.57526141652572 40.0 36.5 41.0 32.0 41.0 46-47 37.37589437167443 40.0 36.0 41.0 32.0 41.0 48-49 37.143941556277085 39.0 35.5 41.0 31.0 41.0 50-51 36.91779508894952 39.0 35.0 41.0 31.0 41.0 52-53 36.71514203528351 39.0 35.0 41.0 31.0 41.0 54-55 36.4924124393526 38.5 35.0 40.5 31.0 41.0 56-57 36.24677547190775 38.0 35.0 40.0 30.5 41.0 58-59 35.94372946013012 37.0 35.0 40.0 30.0 41.0 60-61 35.744721052826506 37.0 35.0 40.0 30.0 41.0 62-63 35.49761015296107 36.0 34.5 39.0 30.0 41.0 64-65 35.22099702472809 36.0 34.0 39.0 30.0 41.0 66-67 34.94005296230911 35.0 34.0 39.0 29.5 41.0 68-69 34.65652374662041 35.0 34.0 37.5 29.0 40.5 70-71 34.4283907606079 35.0 34.0 37.0 29.0 40.0 72-73 34.17592449475932 35.0 34.0 36.5 29.0 39.0 74-75 33.92135825483636 35.0 34.0 36.0 29.0 39.0 76-77 33.697333736620536 35.0 34.0 36.0 29.0 38.5 78-79 33.48778076813866 35.0 33.0 35.0 29.0 37.0 80-81 33.378893100085186 35.0 33.0 35.0 29.0 37.0 82-83 33.25695028456438 35.0 33.0 35.0 29.0 36.5 84-85 33.171409735682275 35.0 33.0 35.0 29.0 36.0 86-87 33.069945432772435 35.0 33.0 35.0 29.0 36.0 88-89 32.99952728978654 35.0 33.0 35.0 29.0 36.0 90-91 32.95564894260565 35.0 33.0 35.0 29.0 35.5 92-93 32.91237243984642 35.0 33.0 35.0 29.0 35.0 94-95 32.888840261231344 35.0 33.0 35.0 29.0 35.0 96-97 32.81518376316342 35.0 33.0 35.0 29.0 35.0 98-99 32.742694534635376 35.0 33.0 35.0 29.0 35.0 100 31.455645979679264 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37854424419384003 1101 2 0.4311872357810884 1101 3 0.33251157322002456 1101 4 0.26029633890719595 1101 5 0.23131232369090782 1101 6 0.2660375891502298 1101 7 0.2782631975262646 1101 8 0.309491294449856 1101 9 0.381221140615402 1101 10-11 0.37412270137877357 1101 12-13 0.40749785269109395 1101 14-15 0.4915226619373385 1101 16-17 0.5470025908396181 1101 18-19 0.5007855578473084 1101 20-21 0.5149000479233408 1101 22-23 0.4594856181713496 1101 24-25 0.5252864424898362 1101 26-27 0.5360269654039271 1101 28-29 0.5397754784301654 1101 30-31 0.576889848229392 1101 32-33 0.661010590732559 1101 34-35 0.6472121997361029 1101 36-37 0.5257679816392908 1101 38-39 0.5682717920077778 1101 40-41 0.5980530818364258 1101 42-43 0.5886846360413287 1101 44-45 0.5484391146115613 1101 46-47 0.5716921322356114 1101 48-49 0.48961491793722445 1101 50-51 0.5491380040073608 1101 52-53 0.5242128822116072 1101 54-55 0.5403006913800965 1101 56-57 0.4910640028051887 1101 58-59 0.5322413915515511 1101 60-61 0.5457080121931028 1101 62-63 0.4938503952385531 1101 64-65 0.5476231297004901 1101 66-67 0.415967486906041 1101 68-69 0.47482465791610196 1101 70-71 0.47445875593579245 1101 72-73 0.5087679301087178 1101 74-75 0.45755323358156375 1101 76-77 0.4526379630231716 1101 78-79 0.4425809947539534 1101 80-81 0.4661243797192398 1101 82-83 0.46664038183713075 1101 84-85 0.4060789349133245 1101 86-87 0.4263809002178647 1101 88-89 0.4447799686713694 1101 90-91 0.3878334525354461 1101 92-93 0.4398015672406217 1101 94-95 0.41725706752478686 1101 96-97 0.4522045112809394 1101 98-99 0.43733291015706044 1101 100 0.7278710629109426 1102 1 0.35355252528522385 1102 2 0.4125007951318054 1102 3 0.3471183635344417 1102 4 0.3030356551146909 1102 5 0.2599533356718666 1102 6 0.24697686347841596 1102 7 0.3386168125385538 1102 8 0.32976894003855506 1102 9 0.3899955436039946 1102 10-11 0.32987169111946457 1102 12-13 0.3527530411784312 1102 14-15 0.48025868533920857 1102 16-17 0.45827174029933104 1102 18-19 0.49936620811831745 1102 20-21 0.5143408204070141 1102 22-23 0.5720940480064769 1102 24-25 0.5149735926729591 1102 26-27 0.5548237876987017 1102 28-29 0.5661774367390464 1102 30-31 0.562368421413133 1102 32-33 0.5639019608650742 1102 34-35 0.5941916942194752 1102 36-37 0.5966588059418356 1102 38-39 0.6085072626975645 1102 40-41 0.5704048136337576 1102 42-43 0.5549390053324217 1102 44-45 0.5258842394067003 1102 46-47 0.5673967532651147 1102 48-49 0.5200415402470213 1102 50-51 0.5563318495769636 1102 52-53 0.5478579647889816 1102 54-55 0.5738471358923789 1102 56-57 0.5253917496736591 1102 58-59 0.501515186094295 1102 60-61 0.47651578470813405 1102 62-63 0.533464191762441 1102 64-65 0.5231239185477818 1102 66-67 0.5430120505726279 1102 68-69 0.49991234553527875 1102 70-71 0.43300171144602473 1102 72-73 0.4863109133730106 1102 74-75 0.4348055042816412 1102 76-77 0.39643579506955007 1102 78-79 0.41396036113989965 1102 80-81 0.37488755415716213 1102 82-83 0.3386434926618236 1102 84-85 0.3217587809256486 1102 86-87 0.35459500727139215 1102 88-89 0.35181858326509285 1102 90-91 0.4172258974047054 1102 92-93 0.41034096413798693 1102 94-95 0.40947891988081864 1102 96-97 0.37940425515377996 1102 98-99 0.3683153443150502 1102 100 0.696056074451441 1103 1 0.2836432641744153 1103 2 0.37051125841937704 1103 3 0.26631611709387215 1103 4 0.24167907802455346 1103 5 0.21815530616443368 1103 6 0.2294364132710669 1103 7 0.253300991068123 1103 8 0.2086690259003916 1103 9 0.2566549329968737 1103 10-11 0.319083660064436 1103 12-13 0.2572545595807725 1103 14-15 0.36170691333691707 1103 16-17 0.39544992493092224 1103 18-19 0.36267336102899606 1103 20-21 0.42225699553852536 1103 22-23 0.3703750682325264 1103 24-25 0.3983013806237494 1103 26-27 0.4579772202738397 1103 28-29 0.4440333781362824 1103 30-31 0.3977989112919005 1103 32-33 0.47810426285939656 1103 34-35 0.49917322959434784 1103 36-37 0.49556648215011023 1103 38-39 0.44660958338742773 1103 40-41 0.4796023107400629 1103 42-43 0.4956747971075828 1103 44-45 0.5290226261766477 1103 46-47 0.5538690312372125 1103 48-49 0.5025319532298127 1103 50-51 0.5275914057864881 1103 52-53 0.4130586521277664 1103 54-55 0.44241080076965034 1103 56-57 0.38768301237114144 1103 58-59 0.4136750776246032 1103 60-61 0.36169591916062416 1103 62-63 0.4126589211732963 1103 64-65 0.4630153717088916 1103 66-67 0.48087913380355474 1103 68-69 0.4615125697485851 1103 70-71 0.47553512862065617 1103 72-73 0.49224530533597033 1103 74-75 0.42117694954710316 1103 76-77 0.43255344332998646 1103 78-79 0.35164980062218376 1103 80-81 0.3086086451731447 1103 82-83 0.32807445980984085 1103 84-85 0.3193668559351863 1103 86-87 0.35189687891293886 1103 88-89 0.4111013732397524 1103 90-91 0.3561337251534695 1103 92-93 0.305894183490679 1103 94-95 0.3535038895338687 1103 96-97 0.39526869813870036 1103 98-99 0.32832973622027595 1103 100 0.5563149599971879 1104 1 0.2287805253523345 1104 2 0.24629200325608736 1104 3 0.1898129773138919 1104 4 0.11260583975408878 1104 5 0.09966872879385846 1104 6 0.18223083253131023 1104 7 0.21041721182155726 1104 8 0.1936680523252008 1104 9 0.1952303620269049 1104 10-11 0.2360923793779719 1104 12-13 0.24951119055399573 1104 14-15 0.2741629301392763 1104 16-17 0.21601869854065825 1104 18-19 0.2829554307464548 1104 20-21 0.34179692956803365 1104 22-23 0.35633404625428255 1104 24-25 0.33206235111450866 1104 26-27 0.29227970426908456 1104 28-29 0.31578512098664646 1104 30-31 0.35680506872417794 1104 32-33 0.3679161754564646 1104 34-35 0.39984265946269204 1104 36-37 0.3009896190352208 1104 38-39 0.30328781915717684 1104 40-41 0.41951710567970935 1104 42-43 0.42951648602790726 1104 44-45 0.36141487272724504 1104 46-47 0.3750157574183248 1104 48-49 0.4210362789622266 1104 50-51 0.41309446737891165 1104 52-53 0.4056968513410695 1104 54-55 0.3899649300423391 1104 56-57 0.3722049707994728 1104 58-59 0.35418828744600006 1104 60-61 0.3599165058818343 1104 62-63 0.3543938386197212 1104 64-65 0.3134396792535341 1104 66-67 0.2910636618414344 1104 68-69 0.2754397889722995 1104 70-71 0.2661322446841794 1104 72-73 0.2720518912705643 1104 74-75 0.3173256358021632 1104 76-77 0.25013926979839596 1104 78-79 0.24906900788212027 1104 80-81 0.23278611952956396 1104 82-83 0.2003715501554666 1104 84-85 0.22885597235539024 1104 86-87 0.27958374719295875 1104 88-89 0.28357258169210553 1104 90-91 0.27407234877662034 1104 92-93 0.29304282946729643 1104 94-95 0.29730388488138715 1104 96-97 0.302200806562233 1104 98-99 0.2718666562591707 1104 100 0.45886519927698544 1105 1 0.19350455667898103 1105 2 0.17176836821547425 1105 3 0.13284370756961295 1105 4 0.17318127105365022 1105 5 0.15326972229968305 1105 6 0.17747736399491032 1105 7 0.2035184859524719 1105 8 0.15892289401234194 1105 9 0.2259811349087002 1105 10-11 0.22456075556649324 1105 12-13 0.22929918166081364 1105 14-15 0.2706648841787853 1105 16-17 0.29712591179401926 1105 18-19 0.28422077597733164 1105 20-21 0.25315116008164296 1105 22-23 0.2398188981844953 1105 24-25 0.31591421207744474 1105 26-27 0.3255884032333043 1105 28-29 0.25942728317451014 1105 30-31 0.33484967002465993 1105 32-33 0.2987075692070533 1105 34-35 0.23449003827968085 1105 36-37 0.3241292504292588 1105 38-39 0.3105451559786019 1105 40-41 0.23397730808119377 1105 42-43 0.3014883449649659 1105 44-45 0.30266288432020616 1105 46-47 0.29987994107840166 1105 48-49 0.28368562267161934 1105 50-51 0.2890520035836843 1105 52-53 0.2868801382825481 1105 54-55 0.2805552556604596 1105 56-57 0.22870823752354852 1105 58-59 0.2674241789293603 1105 60-61 0.2809257639256444 1105 62-63 0.32018520136201545 1105 64-65 0.2998919218252425 1105 66-67 0.25362096258987776 1105 68-69 0.2876463083633425 1105 70-71 0.3273848455050654 1105 72-73 0.18732683667012395 1105 74-75 0.26790841090002004 1105 76-77 0.19738904059540374 1105 78-79 0.24746071088878807 1105 80-81 0.2628040629973114 1105 82-83 0.2645413856977967 1105 84-85 0.23974469081349525 1105 86-87 0.2509016669910267 1105 88-89 0.20788122587499913 1105 90-91 0.21512764380506155 1105 92-93 0.1728696326255914 1105 94-95 0.25215435770623174 1105 96-97 0.20364140035044187 1105 98-99 0.2171184332929741 1105 100 0.3562144550261799 1106 1 0.151516179028107 1106 2 0.20331801476524447 1106 3 0.1344577550149637 1106 4 0.17179011108283504 1106 5 0.12908341252131095 1106 6 0.1744756785027235 1106 7 0.15867634361642757 1106 8 0.10294568589179676 1106 9 0.15370395949172888 1106 10-11 0.1978457043728099 1106 12-13 0.14748501346379328 1106 14-15 0.21121153709317753 1106 16-17 0.18740540161817165 1106 18-19 0.22547256838507224 1106 20-21 0.19609335021522867 1106 22-23 0.16250273003777949 1106 24-25 0.14071836794773418 1106 26-27 0.25091506168214295 1106 28-29 0.2194265284765109 1106 30-31 0.23058650683380222 1106 32-33 0.2967606999458994 1106 34-35 0.28064208476907027 1106 36-37 0.21685169766328727 1106 38-39 0.2632462201027721 1106 40-41 0.21650177287453687 1106 42-43 0.24663613998899336 1106 44-45 0.23017867008660886 1106 46-47 0.28011804829582587 1106 48-49 0.30847028217704064 1106 50-51 0.331451062726849 1106 52-53 0.2864819093071773 1106 54-55 0.2738495124087166 1106 56-57 0.25340899976852427 1106 58-59 0.16848855466834323 1106 60-61 0.20005584870338566 1106 62-63 0.2772334517204982 1106 64-65 0.21013682132462463 1106 66-67 0.25885340957233893 1106 68-69 0.28097653904935527 1106 70-71 0.3382685968572403 1106 72-73 0.2610754216113804 1106 74-75 0.2054316178633684 1106 76-77 0.24710401631928391 1106 78-79 0.25490907696763543 1106 80-81 0.20453106357590656 1106 82-83 0.25013155952990473 1106 84-85 0.2428059974472916 1106 86-87 0.23016042777692292 1106 88-89 0.27679873738414784 1106 90-91 0.2540441490502161 1106 92-93 0.25879137044350387 1106 94-95 0.22446549453297848 1106 96-97 0.16756733932053436 1106 98-99 0.18932554757940778 1106 100 0.27854269834081435 1107 1 0.15210431413162695 1107 2 0.19792787816538748 1107 3 0.20171312873737435 1107 4 0.13922453949439273 1107 5 0.06849356156112663 1107 6 0.061641499647379305 1107 7 0.09731079927982478 1107 8 0.082086294036813 1107 9 0.11659916480785881 1107 10-11 0.18477932973821964 1107 12-13 0.06975366615043299 1107 14-15 0.2285331857790922 1107 16-17 0.18959734541882511 1107 18-19 0.17843639535101374 1107 20-21 0.1358471752508379 1107 22-23 0.15231368300690207 1107 24-25 0.17305049471421796 1107 26-27 0.23111518608175885 1107 28-29 0.21408748907867903 1107 30-31 0.20508298491184007 1107 32-33 0.22447781574225445 1107 34-35 0.1945761277437157 1107 36-37 0.15477166304336265 1107 38-39 0.16968296618248502 1107 40-41 0.20430511046806998 1107 42-43 0.25251100609539634 1107 44-45 0.27201647873578594 1107 46-47 0.30238981728096803 1107 48-49 0.35690168639123954 1107 50-51 0.31666875573233 1107 52-53 0.3014621075643191 1107 54-55 0.36164544406211263 1107 56-57 0.25174826500337133 1107 58-59 0.22420119662078264 1107 60-61 0.20847290785123107 1107 62-63 0.2052136487159757 1107 64-65 0.17784497933988774 1107 66-67 0.20004729254334563 1107 68-69 0.16265246419258972 1107 70-71 0.16400268701793408 1107 72-73 0.1124202747353138 1107 74-75 0.1821098833846193 1107 76-77 0.20931890970276612 1107 78-79 0.19031772210954756 1107 80-81 0.0906426169687009 1107 82-83 0.13296265993653833 1107 84-85 0.1712009270908652 1107 86-87 0.1904411469731926 1107 88-89 0.13095107265244366 1107 90-91 0.12085451030112893 1107 92-93 0.103018900606628 1107 94-95 0.08090092848699726 1107 96-97 0.11246002888800177 1107 98-99 0.1713337685514702 1107 100 0.2758179684305304 1108 1 0.13920532442683964 1108 2 0.12181936383587555 1108 3 0.05139601475718081 1108 4 0.03639604501242388 1108 5 0.0693663768362569 1108 6 0.0742273603523671 1108 7 0.061228445974919055 1108 8 0.05746027430452472 1108 9 0.004601032646156966 1108 10-11 0.04074181664719134 1108 12-13 0.10269563316950325 1108 14-15 0.15263034582479662 1108 16-17 0.10559457611572753 1108 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-0.38399769691905306 2215 7 -0.4716114580081836 2215 8 -0.4855751653944651 2215 9 -0.5493279618938587 2215 10-11 -0.5705531964456583 2215 12-13 -0.5613537695464856 2215 14-15 -0.7015741286723056 2215 16-17 -0.7099328802511238 2215 18-19 -0.6476973424451273 2215 20-21 -0.6785360705200532 2215 22-23 -0.6467208158212401 2215 24-25 -0.692130233234046 2215 26-27 -0.7600078883553962 2215 28-29 -0.668150624426346 2215 30-31 -0.6788048503644433 2215 32-33 -0.6676878045828616 2215 34-35 -0.6947284821674558 2215 36-37 -0.7880351850179466 2215 38-39 -0.9721849152373423 2215 40-41 -0.8835237711377246 2215 42-43 -0.9237983671114307 2215 44-45 -0.9049252627296838 2215 46-47 -0.9126846057171818 2215 48-49 -0.9125070105275057 2215 50-51 -0.9389541525911724 2215 52-53 -0.947978257690508 2215 54-55 -0.9171090044605563 2215 56-57 -0.9964793314751859 2215 58-59 -0.9270387652198622 2215 60-61 -0.934300551908585 2215 62-63 -0.9442354769226995 2215 64-65 -0.8649020186418852 2215 66-67 -1.0308436497842024 2215 68-69 -0.9738594158738394 2215 70-71 -0.936557187308189 2215 72-73 -0.9490977648192285 2215 74-75 -0.8811607221382829 2215 76-77 -0.8617664624706833 2215 78-79 -0.9691846715298738 2215 80-81 -0.9770551308224142 2215 82-83 -0.9139649628106241 2215 84-85 -0.7976893492769221 2215 86-87 -0.7338664772676537 2215 88-89 -0.7960216199114925 2215 90-91 -0.8367742825819562 2215 92-93 -0.854441771679582 2215 94-95 -0.8158112733604668 2215 96-97 -0.7831162958685596 2215 98-99 -0.8805862318840454 2215 100 -0.451818160145109 2216 1 -0.34228399137543164 2216 2 -0.18203997561667862 2216 3 -0.2518964670471533 2216 4 -0.33317903901523493 2216 5 -0.34275128461213455 2216 6 -0.32421381537589866 2216 7 -0.42105547095250273 2216 8 -0.4061211546093517 2216 9 -0.49819386700544754 2216 10-11 -0.4949750459745701 2216 12-13 -0.5118449804176919 2216 14-15 -0.6790010098678749 2216 16-17 -0.6725517687682014 2216 18-19 -0.6414016703743002 2216 20-21 -0.6822893029335262 2216 22-23 -0.5928988333288885 2216 24-25 -0.6888078187299129 2216 26-27 -0.8227692571897549 2216 28-29 -0.6823834086800318 2216 30-31 -0.7085690807438922 2216 32-33 -0.6740942340370211 2216 34-35 -0.6798149169292458 2216 36-37 -0.8098581417113593 2216 38-39 -0.9472457772210845 2216 40-41 -1.0868183513256255 2216 42-43 -0.9997215130168016 2216 44-45 -1.053476428581618 2216 46-47 -0.9705751738749342 2216 48-49 -1.0169290510762394 2216 50-51 -0.9770114742272753 2216 52-53 -0.9764749351395565 2216 54-55 -0.8658111100788233 2216 56-57 -0.8074917944949362 2216 58-59 -0.9365994776282776 2216 60-61 -0.8540899320936148 2216 62-63 -0.9378592695324883 2216 64-65 -0.840127532886001 2216 66-67 -0.8949568249326632 2216 68-69 -0.8441993254054907 2216 70-71 -0.8411620952212857 2216 72-73 -0.8514576441083435 2216 74-75 -0.7271771573469152 2216 76-77 -0.8076520149937991 2216 78-79 -0.7510534456908928 2216 80-81 -0.7961743384449207 2216 82-83 -0.7772063712679582 2216 84-85 -0.7990198364149776 2216 86-87 -0.8452240380537432 2216 88-89 -0.7932946414663746 2216 90-91 -0.8659667156047774 2216 92-93 -0.8340102238016556 2216 94-95 -0.8105990929818176 2216 96-97 -0.798955113346743 2216 98-99 -0.8429525990248123 2216 100 -0.2422601424805535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 5.0 13 30.0 14 130.0 15 435.0 16 1036.0 17 2199.0 18 3874.0 19 5937.0 20 8720.0 21 12458.0 22 17351.0 23 20551.0 24 22812.0 25 25526.0 26 30967.0 27 39855.0 28 50745.0 29 64247.0 30 80810.0 31 100428.0 32 127713.0 33 164767.0 34 229866.0 35 339261.0 36 575966.0 37 1089554.0 38 843332.0 39 191269.0 40 205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.13986247083369 20.15301045666103 12.445204380192838 26.26192269231244 2 15.139736771731297 20.419018947388174 37.74259573267078 26.698648548209746 3 18.253231441587143 25.314724509573956 28.661004185133514 27.771039863705386 4 12.58523356319294 16.35041200752843 37.41330839672924 33.65104603254939 5 13.391837485539607 38.3221127670003 34.44103554324784 13.84501420421225 6 31.73355765994814 37.35010976415829 16.75387964633497 14.162452929558594 7 27.779670759457666 31.357406134445814 22.52679474416106 18.336128361935465 8 27.519042974778092 33.76442266144862 18.896877816323254 19.81965654745003 9 26.463204157973358 14.128640387155714 20.308144343896988 39.10001111097394 10-11 26.7985827335465 24.843940198269156 26.406735719312106 21.950741348872235 12-13 26.73809638740739 22.414548097840477 26.944736245295264 23.902619269456867 14-15 23.99476847101244 23.63287536671139 24.411690662204748 27.960665500071425 16-17 23.2298613597363 26.317897309909753 25.578276811397387 24.873964518956555 18-19 22.578836739425494 26.698171650847506 27.513865888469937 23.209125721257063 20-21 24.2456636337823 26.019555314131924 26.869544820434317 22.865236231651462 22-23 24.553425266354736 25.32916877569413 26.718991123566376 23.398414834384763 24-25 24.182812557869656 25.217528178664463 27.408723349094455 23.190935914371426 26-27 24.09598646930285 25.6864236243997 26.125158948654953 24.0924309576425 28-29 23.168788039653833 26.018580017530645 27.0205553017864 23.792076641029123 30-31 23.936136590906283 25.887038431624298 27.020876285477957 23.15594869199146 32-33 24.24593523536746 25.70834927963852 26.255885729805804 23.789829755188208 34-35 24.112665275737335 25.84100196293873 26.975284255749933 23.071048505574005 36-37 23.89637164973272 25.112751694423523 26.297428426809542 24.69344822903421 38-39 23.999222231824298 25.458895569190503 26.94524758953593 23.596634609449264 40-41 23.613387489043344 24.605264132541574 27.710707275218827 24.07064110319626 42-43 23.737472376884234 24.751015419562723 26.993975383019965 24.517536820533078 44-45 24.146887075468204 25.352464784385376 26.790113702299973 23.710534437846444 46-47 23.94833397118554 25.53922791076653 26.020728139158777 24.49170997888915 48-49 24.88901371588005 25.440488389032232 26.03341934050197 23.63707855458575 50-51 23.988963099220996 25.143171071962072 26.410785052036395 24.457080776780536 52-53 23.781916272638608 25.390785298946927 26.075455858569647 24.75184256984482 54-55 23.619338032863794 25.48161133813163 25.988666806582632 24.910383822421945 56-57 24.732608239404453 25.10450488265577 25.54215380057036 24.620733077369415 58-59 23.312147172522213 25.71185858345682 26.547798983691433 24.428195260329534 60-61 23.570375674374393 25.94556857322749 25.939716793619834 24.544338958778287 62-63 24.1271712694905 25.420821965160922 25.39763706620906 25.054369699139517 64-65 23.262797508966564 25.62632621950467 26.038136356969773 25.072739914558994 66-67 23.9580952959429 25.432436393014253 25.85977716552143 24.749691145521417 68-69 23.45220429377415 25.114726978679276 26.39660004197479 25.036468685571784 70-71 23.17352240819588 25.123714949694275 26.07941015832946 25.623352483780394 72-73 23.778066718896643 24.901439172037193 26.62079383472299 24.699700274343172 74-75 23.88541086681967 25.214670984324826 26.211724096211515 24.68819405264399 76-77 23.668919275104443 25.279111061838687 25.437973474618992 25.61399618843788 78-79 23.668920589736004 25.38564034834636 25.407911681079455 25.53752738083819 80-81 23.15426970037407 25.950975913877606 26.075097838298294 24.81965654745003 82-83 23.984617473858346 25.002098739521735 26.979963210330737 24.03332057628918 84-85 23.916198565449807 25.24597227194726 26.01398748163603 24.8238416809669 86-87 22.688510018394833 25.865767089295193 26.376908927050284 25.06881396525969 88-89 23.744151306773993 24.68468290514932 26.086517450401846 25.48464833767484 90-91 23.2408982605153 25.02387624844138 25.82417501018506 25.91105048085826 92-93 23.508635695855606 25.10388760632585 26.05775237342749 25.329724324391055 94-95 22.634214392414908 25.107541882198987 26.677362007876447 25.580881717509662 96-97 22.936556338810632 25.00924679942223 26.189442105653015 25.864754756114124 98-99 22.4391674176862 25.351699361736273 26.175294132171206 26.033839088406317 100 23.065761597993863 24.798848693353428 25.45314169595375 26.682248012698963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 120.0 1 85.0 2 66.0 3 82.0 4 57.0 5 39.0 6 94.0 7 227.0 8 343.0 9 355.5 10 283.5 11 214.5 12 216.0 13 255.0 14 306.0 15 388.5 16 500.0 17 673.5 18 941.5 19 1265.0 20 1601.5 21 1924.0 22 2392.5 23 2875.0 24 3341.0 25 3842.0 26 4432.5 27 5217.5 28 6292.5 29 8393.5 30 11996.5 31 15911.0 32 22127.5 33 30143.5 34 37933.0 35 45432.0 36 54246.0 37 69242.5 38 81692.0 39 90607.5 40 93626.0 41 93571.0 42 98558.5 43 108540.0 44 125761.0 45 154670.5 46 182581.5 47 204976.0 48 223942.5 49 225442.5 50 216656.0 51 221119.5 52 225622.0 53 218004.0 54 210037.0 55 181827.5 56 156367.5 57 130120.0 58 102513.0 59 99947.5 60 83658.0 61 56482.5 62 40720.0 63 32896.5 64 23868.5 65 12034.0 66 6769.5 67 4698.5 68 3234.0 69 2217.0 70 1491.0 71 920.0 72 510.0 73 300.0 74 156.0 75 73.5 76 25.0 77 6.5 78 2.0 79 0.5 80 1.5 81 2.5 82 1.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.006345600671596647 3 0.0 4 0.08696188935939063 5 0.004814755373390452 6 7.407315959062234E-5 7 4.938210639374823E-5 8 0.0 9 0.0 10-11 0.0 12-13 2.0987395217342996E-4 14-15 0.009222108369032481 16-17 0.0 18-19 7.407315959062234E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.2345526598437058E-5 60-61 0.0 62-63 0.0 64-65 9.876421278749646E-5 66-67 8.64186861890594E-5 68-69 0.0 70-71 3.456747447562376E-4 72-73 3.2098369155936343E-4 74-75 3.703657979531117E-4 76-77 1.1110973938593351E-4 78-79 1.2345526598437058E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.061110356662263436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4050050.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.33823131727772 #Duplication Level Percentage of deduplicated Percentage of total 1 73.70828578939313 48.15969026907383 2 15.62408190818568 20.416997556742594 3 5.416441722858624 10.617021666140733 4 2.334547350131466 6.101407791361099 5 1.1723421924506445 3.829938267667236 6 0.6074978421844934 2.3815700722438504 7 0.3464283743697943 1.584451208160947 8 0.22185720472544518 1.1596605889404623 9 0.144220141661989 0.8480780078869438 >10 0.4162999570615626 3.929382367295776 >50 0.005384940034031892 0.23711502238587023 >100 0.0022413545576774013 0.29522097773146316 >500 1.5909530860809876E-4 0.05668743310365253 >1k 2.1212707678727933E-4 0.38277877126547744 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4204 0.10380118763965876 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.172763299218528E-5 0.0 24-25 0.0 0.0 0.0 2.22219478771867E-4 0.0 26-27 0.0 0.0 0.0 6.296218565202899E-4 0.0 28-29 0.0 0.0 0.0 0.0037530400859248653 0.0 30-31 0.0 0.0 0.0 0.01417266453500574 0.0 32-33 0.0 0.0 0.0 0.03166627572499105 0.0 34-35 0.0 0.0 0.0 0.06165355983259466 0.0 36-37 0.0 0.0 0.0 0.11376402760459747 0.0 38-39 0.0 0.0 0.0 0.19338032863791804 0.0 40-41 0.0 0.0 0.0 0.282922433056382 0.0 42-43 0.0 0.0 0.0 0.3775385489068036 0.0 44-45 0.0 0.0 0.0 0.490537153862298 0.0 46-47 0.0 0.0 0.0 0.6301403686374243 0.0 48-49 0.0 0.0 0.0 0.7950272218861496 0.0 50-51 0.0 0.0 0.0 0.9755805483882914 0.0 52-53 0.0 0.0 0.0 1.1486895223515758 0.0 54-55 0.0 0.0 0.0 1.3433784768089283 0.0 56-57 0.0 0.0 0.0 1.5835977333613167 0.0 58-59 0.0 0.0 0.0 1.868026320662708 0.0 60-61 0.0 0.0 0.0 2.1626893495142037 0.0 62-63 0.0 0.0 0.0 2.4807594967963356 0.0 64-65 0.0 0.0 0.0 2.809693707485093 0.0 66-67 0.0 0.0 0.0 3.1860964679448402 0.0 68-69 0.0 0.0 0.0 3.61121467636202 0.0 70-71 0.0 0.0 0.0 4.0784681670596665 0.0 72-73 0.0 0.0 0.0 4.562844903149344 0.0 74-75 0.0 0.0 0.0 5.028184837224232 0.0 76-77 0.0 0.0 0.0 5.567301638251379 0.0 78-79 0.0 0.0 0.0 6.154084517475093 0.0 80-81 0.0 0.0 0.0 6.778212614659078 0.0 82-83 0.0 0.0 0.0 7.437439043962421 0.0 84-85 0.0 0.0 0.0 8.068468290514932 0.0 86-87 0.0 0.0 0.0 8.740879742225404 0.0 88 0.0 0.0 0.0 9.289366797940767 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4935 0.0 40.98456 94 GTATCAA 10555 0.0 34.631947 1 GGTATCA 6700 0.0 33.70393 1 CTGTGCG 6845 0.0 31.726574 9 GTACTAG 5250 0.0 31.632212 1 TAGTACT 6830 0.0 31.545113 4 GTGGTAT 1660 0.0 31.457848 1 CAACGCA 10760 0.0 31.100216 5 TAGGCAT 6485 0.0 30.2219 5 ATCAACG 11265 0.0 29.81634 3 ACTGTGC 7685 0.0 29.476965 8 CTAACGC 2410 0.0 29.279356 3 TGAACCG 520 0.0 28.922934 5 GTACTGT 7655 0.0 28.609457 6 TACTGTG 8345 0.0 27.87711 7 TATCAAC 13370 0.0 27.410704 2 GAACCGT 550 0.0 27.34363 6 CTAGTAC 8280 0.0 27.327635 3 TAACGCC 2585 0.0 27.297194 4 CTAGGCA 7575 0.0 26.952211 4 >>END_MODULE